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The map label for this gene is ptxB [H]

Identifier: 78358769

GI number: 78358769

Start: 3687543

End: 3688568

Strand: Reverse

Name: ptxB [H]

Synonym: Dde_3730

Alternate gene names: 78358769

Gene position: 3688568-3687543 (Counterclockwise)

Preceding gene: 78358770

Following gene: 78358768

Centisome position: 98.88

GC content: 57.41

Gene sequence:

>1026_bases
ATGTTGAGGAAGTGGACTTGTCTGCTGGCTATCGTGGCCTTCATGGGTCTGGCAACGTCGGCCTTTGCCGCCGACTGCTC
ATTCCGCGGCGCGCTGGACGAAGCGTACTGCGATGAAAACAAAGACCTCGTGGCAGATCCGCCCAAGGATCCCGCCGAAT
TCAAGGACCCCAGCACGCTGGTGTTCACCTATACCCCTGTGGAAGATCCCGCAGTGTATCAGGACGCCTTTGCCGACTTT
CAGGCATATCTTGAAAAGAAAACCGGCAAGCGTGTCATATACTACACGGTGCAGTCCAATGCTGCAGAAGTGGAAGCCAT
GCGCTCCGGCCGTCTGCATATCGCCGGTTTTTCCACGGGTCCCACCTGCTACGCAGTCAACCTTGCCGGCTACATACCCA
TCGCCGTCAAAGGCAATGAAGGCGAATTTCAGGGCTACCGCCTGCTGCTGATCACCCGCAAGGACAGCGATATCAAATCC
GTGGCCGACCTTAAGGGCAAACGTGTAGCGCACACTTCCGCTTCGTCCAACTCCGGCAACCTTGCTCCCCGCGCACTGTT
CCCCAAGCTGGGCATCACCCCCGACAAAGATTACACCGTTGTCTACTCCGGCAAGCATGACCAGTCCATTCTGGGCGTGG
CCTTCGGCGACTACGACGCTGCCCCTGTGGCAGGCGACGTGTTCAAACGTATGGCCAAAGCCGGTCGCATCAACGAAGAA
GACTTCCGTATCGTCTGGCAGAGCGAACAGTTCCCCACTTCCTCCTTCGGGTACGCACACGACCTGAAGCCCGAACTGGT
GGAGCAGATCCGTGAGGCATTCCTTGAATACCGCTTCACCCCTGCCATGCAGAAAACTTTCGGCGGCGCGGACCGTTTCT
TCCCCGTCACGTATCAGAAAGACTGGGCAATCATCCGCGATATAGCCGATGCCAACGGCGAACACTTCAATCAGGCCGGT
CTTGAGGCCATTGCCAAGAAAGAAGCCGAAGCCAAGGCTAAAAAGGCCGCCAAGGCCAAGCAGTAG

Upstream 100 bases:

>100_bases
CGTTATTATTTACCGTACTGCAGGTTTTTCAGGTGACGGTCCGCACCGGGAGGATTCGGGCGACCGTCCACACGGATCGC
ATCATCTCAAGGAGTGCACT

Downstream 100 bases:

>100_bases
GGCTGCCCGGCAGTACCATTTGATAACGGCTTACACAGTGGGCGTCGGTGTTTCCGACGCCCACTGTCCATGTTTTCAGG
GTTTTACACAAAGGAAAGTC

Product: phosphonate-binding periplasmic protein

Products: 3-phospho-serine [Cytoplasm]; phosphate [Cytoplasm]; alkylphosphonate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MLRKWTCLLAIVAFMGLATSAFAADCSFRGALDEAYCDENKDLVADPPKDPAEFKDPSTLVFTYTPVEDPAVYQDAFADF
QAYLEKKTGKRVIYYTVQSNAAEVEAMRSGRLHIAGFSTGPTCYAVNLAGYIPIAVKGNEGEFQGYRLLLITRKDSDIKS
VADLKGKRVAHTSASSNSGNLAPRALFPKLGITPDKDYTVVYSGKHDQSILGVAFGDYDAAPVAGDVFKRMAKAGRINEE
DFRIVWQSEQFPTSSFGYAHDLKPELVEQIREAFLEYRFTPAMQKTFGGADRFFPVTYQKDWAIIRDIADANGEHFNQAG
LEAIAKKEAEAKAKKAAKAKQ

Sequences:

>Translated_341_residues
MLRKWTCLLAIVAFMGLATSAFAADCSFRGALDEAYCDENKDLVADPPKDPAEFKDPSTLVFTYTPVEDPAVYQDAFADF
QAYLEKKTGKRVIYYTVQSNAAEVEAMRSGRLHIAGFSTGPTCYAVNLAGYIPIAVKGNEGEFQGYRLLLITRKDSDIKS
VADLKGKRVAHTSASSNSGNLAPRALFPKLGITPDKDYTVVYSGKHDQSILGVAFGDYDAAPVAGDVFKRMAKAGRINEE
DFRIVWQSEQFPTSSFGYAHDLKPELVEQIREAFLEYRFTPAMQKTFGGADRFFPVTYQKDWAIIRDIADANGEHFNQAG
LEAIAKKEAEAKAKKAAKAKQ
>Mature_341_residues
MLRKWTCLLAIVAFMGLATSAFAADCSFRGALDEAYCDENKDLVADPPKDPAEFKDPSTLVFTYTPVEDPAVYQDAFADF
QAYLEKKTGKRVIYYTVQSNAAEVEAMRSGRLHIAGFSTGPTCYAVNLAGYIPIAVKGNEGEFQGYRLLLITRKDSDIKS
VADLKGKRVAHTSASSNSGNLAPRALFPKLGITPDKDYTVVYSGKHDQSILGVAFGDYDAAPVAGDVFKRMAKAGRINEE
DFRIVWQSEQFPTSSFGYAHDLKPELVEQIREAFLEYRFTPAMQKTFGGADRFFPVTYQKDWAIIRDIADANGEHFNQAG
LEAIAKKEAEAKAKKAAKAKQ

Specific function: Probably forms part of a binding-protein-dependent phosphite transporter. Required for oxidation of phosphite to phosphate [H]

COG id: COG3221

COG function: function code P; ABC-type phosphate/phosphonate transport system, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015168
- InterPro:   IPR005770 [H]

Pfam domain/function: PF09084 NMT1 [H]

EC number: NA

Molecular weight: Translated: 37613; Mature: 37613

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRKWTCLLAIVAFMGLATSAFAADCSFRGALDEAYCDENKDLVADPPKDPAEFKDPSTL
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCEECCCCCCCCCCCCCCEE
VFTYTPVEDPAVYQDAFADFQAYLEKKTGKRVIYYTVQSNAAEVEAMRSGRLHIAGFSTG
EEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHCCCEEEEEECCC
PTCYAVNLAGYIPIAVKGNEGEFQGYRLLLITRKDSDIKSVADLKGKRVAHTSASSNSGN
CEEEEEEECCEEEEEEECCCCCCCCEEEEEEEECCCCHHHHHHCCCCEEEECCCCCCCCC
LAPRALFPKLGITPDKDYTVVYSGKHDQSILGVAFGDYDAAPVAGDVFKRMAKAGRINEE
CCHHHHCCCCCCCCCCCEEEEECCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCCCC
DFRIVWQSEQFPTSSFGYAHDLKPELVEQIREAFLEYRFTPAMQKTFGGADRFFPVTYQK
CEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHCCCCCCEEEEEECC
DWAIIRDIADANGEHFNQAGLEAIAKKEAEAKAKKAAKAKQ
CHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLRKWTCLLAIVAFMGLATSAFAADCSFRGALDEAYCDENKDLVADPPKDPAEFKDPSTL
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCEECCCCCCCCCCCCCCEE
VFTYTPVEDPAVYQDAFADFQAYLEKKTGKRVIYYTVQSNAAEVEAMRSGRLHIAGFSTG
EEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHCCCEEEEEECCC
PTCYAVNLAGYIPIAVKGNEGEFQGYRLLLITRKDSDIKSVADLKGKRVAHTSASSNSGN
CEEEEEEECCEEEEEEECCCCCCCCEEEEEEEECCCCHHHHHHCCCCEEEECCCCCCCCC
LAPRALFPKLGITPDKDYTVVYSGKHDQSILGVAFGDYDAAPVAGDVFKRMAKAGRINEE
CCHHHHCCCCCCCCCCCEEEEECCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCCCC
DFRIVWQSEQFPTSSFGYAHDLKPELVEQIREAFLEYRFTPAMQKTFGGADRFFPVTYQK
CEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHCCCCCCEEEEEECC
DWAIIRDIADANGEHFNQAGLEAIAKKEAEAKAKKAAKAKQ
CHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; 3-phospho-serine [Periplasm]; phosphite [Periplasm]; H2O; phosphate [Periplasm]; alkylphosphonate [Periplasm] [C]

Specific reaction: H2O + ATP + 3-phospho-serine [Periplasm] = ADP + phosphate + 3-phospho-serine [Cytoplasm] ATP + phosphite [Periplasm] + H2O = phosphite [Cytoplasm] + ADP + phosphate phosphate [Periplasm] + ATP + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] A

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9791102 [H]