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The map label for this gene is kdsB

Identifier: 78358686

GI number: 78358686

Start: 3610293

End: 3611042

Strand: Reverse

Name: kdsB

Synonym: Dde_3647

Alternate gene names: 78358686

Gene position: 3611042-3610293 (Counterclockwise)

Preceding gene: 78358690

Following gene: 78358685

Centisome position: 96.8

GC content: 61.47

Gene sequence:

>750_bases
ATGACCGCAACAGCATGTTACGGAATAATCCCGGCACGATACGATTCTTCCCGTTTTCCCGGCAAACCGTTGGCAGATAT
TCAGGGCCGCCCCATGTTCTGGCATGTGTGGCACAGGGCAAGCCTGTGTCCGCAGCTGCAGCAGGTGGTGCTGGCCACGG
ACGACGGACGGATTGCGGAAGCGGCGCACGCGCTGGACGTGCCGTATGTGATGACCCGTTCCGACCACCCCAGCGGCACC
GACCGCGTTTTTGAAGCGGCAACGCTGCTGCAGCTGGACGAGGACGCCGTGGTGGTTAATATTCAGGGTGACGAGCCCGC
ACTGGAGCCGCGCATGCTTTCCGAGCTGGTGCGGCCTTTTGCTGAAGATGCCGCCGTGCAGGTAACCACTCTGGCGCGGG
CCATCAGCCCGCAGCAGGCCGCCTGTCCCGATGTGGTCAAGGTGGTGTGCACGGCGGGGGGTGACGCTCTGTATTTTTCG
CGGGCAGCCATACCGTACTGCCGCGACGGGCAGTCCGGTGCGCCCTGCATGGGGCACGTGGGGCTGTATGCTTTCCGGTA
TCAGGCTCTGCGGCGTTTTACGCAGCTGGAACAGTCCGTGCTTGAGCGTACGGAAAAACTGGAGCAGCTGCGGCTTCTGG
AAAACAATATACCCATACGGGTGGTGGAAACGGCATACCGGACGCACGGTGTGGACCGCCCCGGGGATATCGACGTAATT
ATCAACATGATACGGGAGAACGAAGGATGA

Upstream 100 bases:

>100_bases
AGCTCTTTCCTCCTGCGGCGTTTGCAGGTATAGTCCATCGATTTGGCGCACTGTTACCGGGCGGCACCCGCCGCCACGCA
GCTATATTCCGGCAGGACTC

Downstream 100 bases:

>100_bases
GAGCGCTGCTGGCCCTTGAAGACGGCTTCGTACTGGAAGGCCGTTCCTTTACCGGCCCCGGAGAGACGGGTGGTGAGGTG
ATTTTCAACACCGGCATGAC

Product: 3-deoxy-manno-octulosonate cytidylyltransferase

Products: NA

Alternate protein names: CMP-2-keto-3-deoxyoctulosonic acid synthase; CKS; CMP-KDO synthase

Number of amino acids: Translated: 249; Mature: 248

Protein sequence:

>249_residues
MTATACYGIIPARYDSSRFPGKPLADIQGRPMFWHVWHRASLCPQLQQVVLATDDGRIAEAAHALDVPYVMTRSDHPSGT
DRVFEAATLLQLDEDAVVVNIQGDEPALEPRMLSELVRPFAEDAAVQVTTLARAISPQQAACPDVVKVVCTAGGDALYFS
RAAIPYCRDGQSGAPCMGHVGLYAFRYQALRRFTQLEQSVLERTEKLEQLRLLENNIPIRVVETAYRTHGVDRPGDIDVI
INMIRENEG

Sequences:

>Translated_249_residues
MTATACYGIIPARYDSSRFPGKPLADIQGRPMFWHVWHRASLCPQLQQVVLATDDGRIAEAAHALDVPYVMTRSDHPSGT
DRVFEAATLLQLDEDAVVVNIQGDEPALEPRMLSELVRPFAEDAAVQVTTLARAISPQQAACPDVVKVVCTAGGDALYFS
RAAIPYCRDGQSGAPCMGHVGLYAFRYQALRRFTQLEQSVLERTEKLEQLRLLENNIPIRVVETAYRTHGVDRPGDIDVI
INMIRENEG
>Mature_248_residues
TATACYGIIPARYDSSRFPGKPLADIQGRPMFWHVWHRASLCPQLQQVVLATDDGRIAEAAHALDVPYVMTRSDHPSGTD
RVFEAATLLQLDEDAVVVNIQGDEPALEPRMLSELVRPFAEDAAVQVTTLARAISPQQAACPDVVKVVCTAGGDALYFSR
AAIPYCRDGQSGAPCMGHVGLYAFRYQALRRFTQLEQSVLERTEKLEQLRLLENNIPIRVVETAYRTHGVDRPGDIDVII
NMIRENEG

Specific function: Activates KDO (a required 8-carbon sugar) for incorporation into bacterial lipopolysaccharide in Gram-negative bacteria

COG id: COG1212

COG function: function code M; CMP-2-keto-3-deoxyoctulosonic acid synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the kdsB family

Homologues:

Organism=Escherichia coli, GI1787147, Length=238, Percent_Identity=40.7563025210084, Blast_Score=152, Evalue=2e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KDSB_DESDG (Q30V56)

Other databases:

- EMBL:   CP000112
- RefSeq:   YP_390135.1
- ProteinModelPortal:   Q30V56
- SMR:   Q30V56
- STRING:   Q30V56
- GeneID:   3758634
- GenomeReviews:   CP000112_GR
- KEGG:   dde:Dde_3647
- eggNOG:   COG1212
- HOGENOM:   HBG637773
- OMA:   NTRQDAL
- ProtClustDB:   PRK05450
- BioCyc:   DDES207559:DDE_3647-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00057
- InterPro:   IPR003329
- InterPro:   IPR004528
- TIGRFAMs:   TIGR00466

Pfam domain/function: PF02348 CTP_transf_3

EC number: =2.7.7.38

Molecular weight: Translated: 27562; Mature: 27430

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTATACYGIIPARYDSSRFPGKPLADIQGRPMFWHVWHRASLCPQLQQVVLATDDGRIAE
CCCCCCCCCEECCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHCHHHHHHEEECCCCCHHH
AAHALDVPYVMTRSDHPSGTDRVFEAATLLQLDEDAVVVNIQGDEPALEPRMLSELVRPF
HHHHCCCCEEEECCCCCCCHHHHHHHHHHEEECCCEEEEEECCCCCCCCHHHHHHHHHHH
AEDAAVQVTTLARAISPQQAACPDVVKVVCTAGGDALYFSRAAIPYCRDGQSGAPCMGHV
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCHHHHH
GLYAFRYQALRRFTQLEQSVLERTEKLEQLRLLENNIPIRVVETAYRTHGVDRPGDIDVI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHCCCCCCCCHHHH
INMIRENEG
HHHHHCCCC
>Mature Secondary Structure 
TATACYGIIPARYDSSRFPGKPLADIQGRPMFWHVWHRASLCPQLQQVVLATDDGRIAE
CCCCCCCCEECCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHCHHHHHHEEECCCCCHHH
AAHALDVPYVMTRSDHPSGTDRVFEAATLLQLDEDAVVVNIQGDEPALEPRMLSELVRPF
HHHHCCCCEEEECCCCCCCHHHHHHHHHHEEECCCEEEEEECCCCCCCCHHHHHHHHHHH
AEDAAVQVTTLARAISPQQAACPDVVKVVCTAGGDALYFSRAAIPYCRDGQSGAPCMGHV
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCHHHHH
GLYAFRYQALRRFTQLEQSVLERTEKLEQLRLLENNIPIRVVETAYRTHGVDRPGDIDVI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHCCCCCCCCHHHH
INMIRENEG
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA