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The map label for this gene is aegA [H]

Identifier: 78358674

GI number: 78358674

Start: 3598159

End: 3599589

Strand: Reverse

Name: aegA [H]

Synonym: Dde_3635

Alternate gene names: 78358674

Gene position: 3599589-3598159 (Counterclockwise)

Preceding gene: 78358675

Following gene: 78358666

Centisome position: 96.5

GC content: 60.73

Gene sequence:

>1431_bases
ATGAAAGCAAGTAAAAAGCCCGTACTGCCCCGTGTGCCCATGCCCAACCAGCCGCCCGCGGAACGCGTGCGTAATTTTGA
CGAAGTGGCGCTGGGGTATACGCCGGAAATGGCGCGGCAGGAGGCCGGGCGCTGTCTGCAGTGCAAAAAGCCCCGCTGCG
TCAAGGGCTGCCCTGTGGAAGTGCCCATACCGCAGTTTATCGCCGCGCTGGCGCAGGGCGATGTGGAACATGCCTACCGG
ATTATCAAAACCACCAACAGCCTGCCTGCCGTGTGCGGGCGGGTGTGCCCTCAGGAATCGCAGTGCGAGGGGGCGTGCAT
ACTGCAGGCAAAAGGGCAGCCGGTGGCCATCGGGCGTCTGGAACGTTTTGTGGCCGACGAATACATGCATCGTGACGCCT
GCGGTCTGATAACCGGTAAACCGGAATGCCCTTACATTGACCCGGAAAAAAAGGTCGCCTGTATCGGCAGCGGGCCTGCC
AGCCTCACTGCGGCGGGGTATCTGGCCGCGCACGGGTGCAAGGTTACTGTTTTCGAGGCCCTGCACGAACTGGGCGGCGT
GCTTGTGTACGGTATTCCCGAATTCCGTCTGCCCAAGGAAAAGATTGTCGCCAAGGAAGTGAACGCCCTGCGTGAACTGC
AGGTGGAATTTGTCCGCAACAGAGTGGGCGGCAAAACATTTTCCATAAGCGATCTTTTCGCACAGGGGTACAAGGCGGTC
TTTATCGGAGTCGGGGCGGGGCTGCCGCGTTTTCTCGGAGTACCGGGAGAAAATCTCAACGGCGTGTTTTCCGCCAATGA
GTATCTTACCCGTGTGAATCTGGGCCGGGCGTATTCTTTTCCGGAATATGACACCCCCATTATCCGCGGCCGCCGCGTGA
CTGTTTTCGGCGGGGGCAACGTGGCCATGGATGCGGCCCGTACAGCCATGAGGCTGGGCGCCGAATCTGTGCATATCGTC
TATCGCCGTACGCAGGCAGAAATGCCTGCACGCCACGAAGAAGTGGAACACGCCATTGAAGAAGGCGTGCAGGTGGCCGA
ACTGTGCGGTCCTCTGGAATTCCGTGCCGGTGAAAACGGCAGTCTGGCAGCCGTTGTGCTGCAGAAAATGGAGCTGGGGG
AACCCGATGAGTCCGGTCGCCGCCGGCCGGTGGCCGTGCAGGGTGCCACCGTGGAACTGCCCACAGACCTTGCCGTCATT
GCGGTCGGCACACGGTCCAACCCCGTACTGCTGGAAAATGAACCTGAATTGAAGCTGACGGGCAGGGGCTATGTGTCGGT
GGACGAGGAAACCGGCGAAACAAGCATGCCCGGCGTATATGCCGGTGGTGATATAGTGACCGGTGCCGCCACGGTTATTC
TGGCCATGGGGGCCGGTCGCAGGGCGGCCAAGCACATTGCCCGTTCACTGGGGTGCGCGGTGGAAGACTAG

Upstream 100 bases:

>100_bases
GACGGACACAAGGTGGATTTTGCGGAACTGCGGCAGCGGCTGGGCGCATTCCGCCCGCTGGAGCGTGAATCCTACGACCA
TCACTGCAGGTGCAAGTGTA

Downstream 100 bases:

>100_bases
CGTATCTGTCTGTATCTGCAGCATACTTTCAGCGCCCGCGGCAACGCCGCGGGCGCTTTTGCTGTCTCTGCCGTCCGTTG
ATCCGGCTGCCGCAGGTGGT

Product: putative oxidoreductase

Products: L-glutamine; 2-oxoglutarate; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MKASKKPVLPRVPMPNQPPAERVRNFDEVALGYTPEMARQEAGRCLQCKKPRCVKGCPVEVPIPQFIAALAQGDVEHAYR
IIKTTNSLPAVCGRVCPQESQCEGACILQAKGQPVAIGRLERFVADEYMHRDACGLITGKPECPYIDPEKKVACIGSGPA
SLTAAGYLAAHGCKVTVFEALHELGGVLVYGIPEFRLPKEKIVAKEVNALRELQVEFVRNRVGGKTFSISDLFAQGYKAV
FIGVGAGLPRFLGVPGENLNGVFSANEYLTRVNLGRAYSFPEYDTPIIRGRRVTVFGGGNVAMDAARTAMRLGAESVHIV
YRRTQAEMPARHEEVEHAIEEGVQVAELCGPLEFRAGENGSLAAVVLQKMELGEPDESGRRRPVAVQGATVELPTDLAVI
AVGTRSNPVLLENEPELKLTGRGYVSVDEETGETSMPGVYAGGDIVTGAATVILAMGAGRRAAKHIARSLGCAVED

Sequences:

>Translated_476_residues
MKASKKPVLPRVPMPNQPPAERVRNFDEVALGYTPEMARQEAGRCLQCKKPRCVKGCPVEVPIPQFIAALAQGDVEHAYR
IIKTTNSLPAVCGRVCPQESQCEGACILQAKGQPVAIGRLERFVADEYMHRDACGLITGKPECPYIDPEKKVACIGSGPA
SLTAAGYLAAHGCKVTVFEALHELGGVLVYGIPEFRLPKEKIVAKEVNALRELQVEFVRNRVGGKTFSISDLFAQGYKAV
FIGVGAGLPRFLGVPGENLNGVFSANEYLTRVNLGRAYSFPEYDTPIIRGRRVTVFGGGNVAMDAARTAMRLGAESVHIV
YRRTQAEMPARHEEVEHAIEEGVQVAELCGPLEFRAGENGSLAAVVLQKMELGEPDESGRRRPVAVQGATVELPTDLAVI
AVGTRSNPVLLENEPELKLTGRGYVSVDEETGETSMPGVYAGGDIVTGAATVILAMGAGRRAAKHIARSLGCAVED
>Mature_476_residues
MKASKKPVLPRVPMPNQPPAERVRNFDEVALGYTPEMARQEAGRCLQCKKPRCVKGCPVEVPIPQFIAALAQGDVEHAYR
IIKTTNSLPAVCGRVCPQESQCEGACILQAKGQPVAIGRLERFVADEYMHRDACGLITGKPECPYIDPEKKVACIGSGPA
SLTAAGYLAAHGCKVTVFEALHELGGVLVYGIPEFRLPKEKIVAKEVNALRELQVEFVRNRVGGKTFSISDLFAQGYKAV
FIGVGAGLPRFLGVPGENLNGVFSANEYLTRVNLGRAYSFPEYDTPIIRGRRVTVFGGGNVAMDAARTAMRLGAESVHIV
YRRTQAEMPARHEEVEHAIEEGVQVAELCGPLEFRAGENGSLAAVVLQKMELGEPDESGRRRPVAVQGATVELPTDLAVI
AVGTRSNPVLLENEPELKLTGRGYVSVDEETGETSMPGVYAGGDIVTGAATVILAMGAGRRAAKHIARSLGCAVED

Specific function: Unknown

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=486, Percent_Identity=31.4814814814815, Blast_Score=188, Evalue=1e-47,
Organism=Homo sapiens, GI237757300, Length=131, Percent_Identity=33.587786259542, Blast_Score=70, Evalue=4e-12,
Organism=Escherichia coli, GI1788811, Length=487, Percent_Identity=35.3182751540041, Blast_Score=274, Evalue=1e-74,
Organism=Escherichia coli, GI1789606, Length=469, Percent_Identity=35.8208955223881, Blast_Score=272, Evalue=4e-74,
Organism=Escherichia coli, GI87082180, Length=474, Percent_Identity=37.1308016877637, Blast_Score=270, Evalue=2e-73,
Organism=Escherichia coli, GI1789243, Length=425, Percent_Identity=33.6470588235294, Blast_Score=174, Evalue=1e-44,
Organism=Escherichia coli, GI1788468, Length=459, Percent_Identity=30.5010893246187, Blast_Score=166, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI71984108, Length=449, Percent_Identity=31.8485523385301, Blast_Score=171, Evalue=9e-43,
Organism=Caenorhabditis elegans, GI17570289, Length=481, Percent_Identity=27.6507276507277, Blast_Score=149, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6320030, Length=478, Percent_Identity=29.4979079497908, Blast_Score=162, Evalue=8e-41,
Organism=Drosophila melanogaster, GI28574881, Length=484, Percent_Identity=30.7851239669421, Blast_Score=187, Evalue=2e-47,
Organism=Drosophila melanogaster, GI24665539, Length=484, Percent_Identity=30.7851239669421, Blast_Score=187, Evalue=2e-47,
Organism=Drosophila melanogaster, GI24665547, Length=484, Percent_Identity=30.5785123966942, Blast_Score=187, Evalue=2e-47,
Organism=Drosophila melanogaster, GI24665543, Length=484, Percent_Identity=30.5785123966942, Blast_Score=187, Evalue=2e-47,
Organism=Drosophila melanogaster, GI24640763, Length=468, Percent_Identity=30.7692307692308, Blast_Score=167, Evalue=1e-41,
Organism=Drosophila melanogaster, GI18858217, Length=468, Percent_Identity=30.7692307692308, Blast_Score=167, Evalue=1e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006 [H]

Pfam domain/function: PF00037 Fer4; PF07992 Pyr_redox_2 [H]

EC number: 1.4.1.13

Molecular weight: Translated: 51058; Mature: 51058

Theoretical pI: Translated: 7.12; Mature: 7.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKASKKPVLPRVPMPNQPPAERVRNFDEVALGYTPEMARQEAGRCLQCKKPRCVKGCPVE
CCCCCCCCCCCCCCCCCCHHHHHCCHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCCC
VPIPQFIAALAQGDVEHAYRIIKTTNSLPAVCGRVCPQESQCEGACILQAKGQPVAIGRL
CCHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCEEEEEECCCEEEHHHH
ERFVADEYMHRDACGLITGKPECPYIDPEKKVACIGSGPASLTAAGYLAAHGCKVTVFEA
HHHHHHHHHHCCCCEEEECCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCEEHHHHH
LHELGGVLVYGIPEFRLPKEKIVAKEVNALRELQVEFVRNRVGGKTFSISDLFAQGYKAV
HHHHCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHCCCEEE
FIGVGAGLPRFLGVPGENLNGVFSANEYLTRVNLGRAYSFPEYDTPIIRGRRVTVFGGGN
EEECCCCCHHHHCCCCCCCCCEEECCHHHEEEECCCCCCCCCCCCCEECCCEEEEEECCC
VAMDAARTAMRLGAESVHIVYRRTQAEMPARHEEVEHAIEEGVQVAELCGPLEFRAGENG
EEHHHHHHHHHHCCCEEEEEEEEHHHCCCHHHHHHHHHHHHCCHHHHHCCCCEECCCCCC
SLAAVVLQKMELGEPDESGRRRPVAVQGATVELPTDLAVIAVGTRSNPVLLENEPELKLT
CCHHHHHHHHHCCCCCCCCCCCCEEECCCEEECCCCEEEEEECCCCCCEEEECCCCEEEE
GRGYVSVDEETGETSMPGVYAGGDIVTGAATVILAMGAGRRAAKHIARSLGCAVED
CCCEEEECCCCCCCCCCCEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKASKKPVLPRVPMPNQPPAERVRNFDEVALGYTPEMARQEAGRCLQCKKPRCVKGCPVE
CCCCCCCCCCCCCCCCCCHHHHHCCHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCCC
VPIPQFIAALAQGDVEHAYRIIKTTNSLPAVCGRVCPQESQCEGACILQAKGQPVAIGRL
CCHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCEEEEEECCCEEEHHHH
ERFVADEYMHRDACGLITGKPECPYIDPEKKVACIGSGPASLTAAGYLAAHGCKVTVFEA
HHHHHHHHHHCCCCEEEECCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCEEHHHHH
LHELGGVLVYGIPEFRLPKEKIVAKEVNALRELQVEFVRNRVGGKTFSISDLFAQGYKAV
HHHHCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHCCCEEE
FIGVGAGLPRFLGVPGENLNGVFSANEYLTRVNLGRAYSFPEYDTPIIRGRRVTVFGGGN
EEECCCCCHHHHCCCCCCCCCEEECCHHHEEEECCCCCCCCCCCCCEECCCEEEEEECCC
VAMDAARTAMRLGAESVHIVYRRTQAEMPARHEEVEHAIEEGVQVAELCGPLEFRAGENG
EEHHHHHHHHHHCCCEEEEEEEEHHHCCCHHHHHHHHHHHHCCHHHHHCCCCEECCCCCC
SLAAVVLQKMELGEPDESGRRRPVAVQGATVELPTDLAVIAVGTRSNPVLLENEPELKLT
CCHHHHHHHHHCCCCCCCCCCCCEEECCCEEECCCCEEEEEECCCCCCEEEECCCCEEEE
GRGYVSVDEETGETSMPGVYAGGDIVTGAATVILAMGAGRRAAKHIARSLGCAVED
CCCEEEECCCCCCCCCCCEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-glutamate; NADP+

Specific reaction: 2 L-glutamate + NADP+ = L-glutamine + 2-oxoglutarate + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8955321; 9205837; 9278503 [H]