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The map label for this gene is ansB [H]

Identifier: 78358591

GI number: 78358591

Start: 3512532

End: 3514004

Strand: Direct

Name: ansB [H]

Synonym: Dde_3552

Alternate gene names: 78358591

Gene position: 3512532-3514004 (Clockwise)

Preceding gene: 78358590

Following gene: 78358592

Centisome position: 94.16

GC content: 64.15

Gene sequence:

>1473_bases
ATGCGAACTGAACATGACGCGCTGGGCAGCAGGGAACTGCCCGCCGATGCGCTGTACGGCATACACACGCTGCGTGCCGC
AGAAAATTTTCCCGTAAGCCCCTACCGCCTTGCCCCCCCGCTCATCCGTGCTCTTGCTCAGGTCAAGCTGGCCTGCGCAC
GCACCAACGCCCGTCTGGGGTATCTGGACAACCGGCTGGCAGAAGCCATCGAAACCGCCTGCCTGGAAATCATCGGCGGC
AGCCATCAGGACAGCTTCATCACAGACCCGTTTCAGGGCGGGGCCGGCACCTCGGCCAACATGAACATGAACGAGGTGGT
GGCCAACCGCGCCTGCCAGCTGCTCGGAGGCGTTCCCGGACAGCATTCCCTGGTCAGCCCCCTGAATCATGTCAACATGC
ACCAGTCCACCAATGACGTTTTTCCCACCGCACTGCGCGTGGCCATGCTGCACGAACTGACTGCGCTGGAAAAAGAGACC
GCCGCCCTGCAGGCGCAGCTGCAGCAGCTTGAAGCCGCACATCAGGACACGCTGCGTCTGGGCCGGACCCAGCTGCAGGA
CGCTGTTCCCATGACGTTCGGCATGACATTCGGGGCATGGGCAGAAGCAGTGGCCCGCGACAGATGGCGGGTTTTCAAAT
CCAGAGAACGCATACGGCAGGTCAGTCTGGGCGGCACGGCCATCGGCACCGGTCTGGGAGCCCCCCGCGACTACATACTG
AAAGCTGCAGACACCCTGCGCAGCGTTACGGGACTCAAACTTTCCAGAGCGGAAAATCTGGTGGATGCCACCCAGAACAC
CGACCAGCTGGTCGAGGTTTCCGCGATTCTCAAGGCGCTGGCAGCCAATCTGCTTAAAATCAGCACGGACCTGCGGCTGC
TGGGCAGCGGCCCCTGTGCCGGACTGGGCGAGCTGAAACTGCCCGCCATGCAGGCCGGTTCCACCATCATGCCCGGCAAA
GTGAACCCTGTCATCGCCGAAGCCGTTTCACAGGCGGCGCTGCGGGTGCTGGCCAACGACGGTCTTGCCGCGCATGCTTC
GGCCATGGGCACGCTGGAACTGAACCAGTTCATGCCGCTGCTGGCGCACACCCTGCTCGAGTCGCTGCACCTGATGCGCA
GCAGCGTGACGCTGCTCAACAACCGCTGCATGCAGGGCCTTGAGCCGCAGGCGGAACAGGCACAAAGCCATGTGGGACGT
TCCGCCGCACTGGCGGCGGTGCTGGTGCCGCTGGCCGGCCACACTGCCGTGGAGCAGATGCTGCACACGGCCCGGACCAC
CGGCACTCCGTTTGCCCGGGTGGCGGCGCAGGCTCTCTGCGTGCCGGAAGACAACATCCGCAGCCTGCTGGCACCGGAGC
GTATGCGCAAGCTGGGCTTCACACCGCAGGACCTGCCGCAGCAATGCTCCGGCCGCACAGGACACACGGTACCGGACAAG
GCTCTGCCGGACGAATCCAACAACGGAGAATGA

Upstream 100 bases:

>100_bases
CCGCCCGTGCCGTTCTGATAACCGCGCCGTTCTGTTGAACCGCATGGCCGCCCGTGTTACGGTACCGACATCCTGAAAAA
GTGAACAGGCACGGAGGACT

Downstream 100 bases:

>100_bases
TCCATGTATGTAGGCATCCATACGGCAAGCCTGCCGTTTGTCTTTGCAGAAGCCCGCAGGCGCATCATCATGCACGCCGC
ACTTTACTCCAACTTCGCCC

Product: aspartate ammonia-lyase

Products: NA

Alternate protein names: Aspartase [H]

Number of amino acids: Translated: 490; Mature: 490

Protein sequence:

>490_residues
MRTEHDALGSRELPADALYGIHTLRAAENFPVSPYRLAPPLIRALAQVKLACARTNARLGYLDNRLAEAIETACLEIIGG
SHQDSFITDPFQGGAGTSANMNMNEVVANRACQLLGGVPGQHSLVSPLNHVNMHQSTNDVFPTALRVAMLHELTALEKET
AALQAQLQQLEAAHQDTLRLGRTQLQDAVPMTFGMTFGAWAEAVARDRWRVFKSRERIRQVSLGGTAIGTGLGAPRDYIL
KAADTLRSVTGLKLSRAENLVDATQNTDQLVEVSAILKALAANLLKISTDLRLLGSGPCAGLGELKLPAMQAGSTIMPGK
VNPVIAEAVSQAALRVLANDGLAAHASAMGTLELNQFMPLLAHTLLESLHLMRSSVTLLNNRCMQGLEPQAEQAQSHVGR
SAALAAVLVPLAGHTAVEQMLHTARTTGTPFARVAAQALCVPEDNIRSLLAPERMRKLGFTPQDLPQQCSGRTGHTVPDK
ALPDESNNGE

Sequences:

>Translated_490_residues
MRTEHDALGSRELPADALYGIHTLRAAENFPVSPYRLAPPLIRALAQVKLACARTNARLGYLDNRLAEAIETACLEIIGG
SHQDSFITDPFQGGAGTSANMNMNEVVANRACQLLGGVPGQHSLVSPLNHVNMHQSTNDVFPTALRVAMLHELTALEKET
AALQAQLQQLEAAHQDTLRLGRTQLQDAVPMTFGMTFGAWAEAVARDRWRVFKSRERIRQVSLGGTAIGTGLGAPRDYIL
KAADTLRSVTGLKLSRAENLVDATQNTDQLVEVSAILKALAANLLKISTDLRLLGSGPCAGLGELKLPAMQAGSTIMPGK
VNPVIAEAVSQAALRVLANDGLAAHASAMGTLELNQFMPLLAHTLLESLHLMRSSVTLLNNRCMQGLEPQAEQAQSHVGR
SAALAAVLVPLAGHTAVEQMLHTARTTGTPFARVAAQALCVPEDNIRSLLAPERMRKLGFTPQDLPQQCSGRTGHTVPDK
ALPDESNNGE
>Mature_490_residues
MRTEHDALGSRELPADALYGIHTLRAAENFPVSPYRLAPPLIRALAQVKLACARTNARLGYLDNRLAEAIETACLEIIGG
SHQDSFITDPFQGGAGTSANMNMNEVVANRACQLLGGVPGQHSLVSPLNHVNMHQSTNDVFPTALRVAMLHELTALEKET
AALQAQLQQLEAAHQDTLRLGRTQLQDAVPMTFGMTFGAWAEAVARDRWRVFKSRERIRQVSLGGTAIGTGLGAPRDYIL
KAADTLRSVTGLKLSRAENLVDATQNTDQLVEVSAILKALAANLLKISTDLRLLGSGPCAGLGELKLPAMQAGSTIMPGK
VNPVIAEAVSQAALRVLANDGLAAHASAMGTLELNQFMPLLAHTLLESLHLMRSSVTLLNNRCMQGLEPQAEQAQSHVGR
SAALAAVLVPLAGHTAVEQMLHTARTTGTPFARVAAQALCVPEDNIRSLLAPERMRKLGFTPQDLPQQCSGRTGHTVPDK
ALPDESNNGE

Specific function: Unknown

COG id: COG1027

COG function: function code E; Aspartate ammonia-lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Aspartase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=462, Percent_Identity=35.0649350649351, Blast_Score=267, Evalue=1e-71,
Organism=Escherichia coli, GI87082375, Length=417, Percent_Identity=41.726618705036, Blast_Score=318, Evalue=5e-88,
Organism=Escherichia coli, GI1787896, Length=456, Percent_Identity=37.719298245614, Blast_Score=299, Evalue=3e-82,
Organism=Caenorhabditis elegans, GI17553882, Length=462, Percent_Identity=37.012987012987, Blast_Score=296, Evalue=2e-80,
Organism=Caenorhabditis elegans, GI32565146, Length=328, Percent_Identity=35.3658536585366, Blast_Score=198, Evalue=6e-51,
Organism=Saccharomyces cerevisiae, GI6324993, Length=465, Percent_Identity=35.4838709677419, Blast_Score=281, Evalue=2e-76,
Organism=Drosophila melanogaster, GI24662684, Length=459, Percent_Identity=37.6906318082789, Blast_Score=293, Evalue=2e-79,
Organism=Drosophila melanogaster, GI24640179, Length=459, Percent_Identity=35.2941176470588, Blast_Score=291, Evalue=1e-78,
Organism=Drosophila melanogaster, GI24640177, Length=459, Percent_Identity=35.2941176470588, Blast_Score=290, Evalue=2e-78,
Organism=Drosophila melanogaster, GI78710009, Length=460, Percent_Identity=35, Blast_Score=285, Evalue=4e-77,
Organism=Drosophila melanogaster, GI24583245, Length=459, Percent_Identity=33.9869281045752, Blast_Score=266, Evalue=3e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004708
- InterPro:   IPR003031
- InterPro:   IPR005677
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.3.1.1 [H]

Molecular weight: Translated: 52339; Mature: 52339

Theoretical pI: Translated: 7.23; Mature: 7.23

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTEHDALGSRELPADALYGIHTLRAAENFPVSPYRLAPPLIRALAQVKLACARTNARLG
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHCCHHHHHHHHHHHHHHHHCCCCHH
YLDNRLAEAIETACLEIIGGSHQDSFITDPFQGGAGTSANMNMNEVVANRACQLLGGVPG
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCC
QHSLVSPLNHVNMHQSTNDVFPTALRVAMLHELTALEKETAALQAQLQQLEAAHQDTLRL
CHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GRTQLQDAVPMTFGMTFGAWAEAVARDRWRVFKSRERIRQVSLGGTAIGTGLGAPRDYIL
HHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCHHHHH
KAADTLRSVTGLKLSRAENLVDATQNTDQLVEVSAILKALAANLLKISTDLRLLGSGPCA
HHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCHHEECCCCCC
GLGELKLPAMQAGSTIMPGKVNPVIAEAVSQAALRVLANDGLAAHASAMGTLELNQFMPL
CCCCCCCCHHHCCCEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
LAHTLLESLHLMRSSVTLLNNRCMQGLEPQAEQAQSHVGRSAALAAVLVPLAGHTAVEQM
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCHHHHHHHHHHHHCCHHHHHHH
LHTARTTGTPFARVAAQALCVPEDNIRSLLAPERMRKLGFTPQDLPQQCSGRTGHTVPDK
HHHHHCCCCHHHHHHHHHHCCCHHHHHHHHCHHHHHHCCCCCCHHHHHHCCCCCCCCCCC
ALPDESNNGE
CCCCCCCCCC
>Mature Secondary Structure
MRTEHDALGSRELPADALYGIHTLRAAENFPVSPYRLAPPLIRALAQVKLACARTNARLG
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHCCHHHHHHHHHHHHHHHHCCCCHH
YLDNRLAEAIETACLEIIGGSHQDSFITDPFQGGAGTSANMNMNEVVANRACQLLGGVPG
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCC
QHSLVSPLNHVNMHQSTNDVFPTALRVAMLHELTALEKETAALQAQLQQLEAAHQDTLRL
CHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GRTQLQDAVPMTFGMTFGAWAEAVARDRWRVFKSRERIRQVSLGGTAIGTGLGAPRDYIL
HHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCHHHHH
KAADTLRSVTGLKLSRAENLVDATQNTDQLVEVSAILKALAANLLKISTDLRLLGSGPCA
HHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCHHEECCCCCC
GLGELKLPAMQAGSTIMPGKVNPVIAEAVSQAALRVLANDGLAAHASAMGTLELNQFMPL
CCCCCCCCHHHCCCEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
LAHTLLESLHLMRSSVTLLNNRCMQGLEPQAEQAQSHVGRSAALAAVLVPLAGHTAVEQM
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCHHHHHHHHHHHHCCHHHHHHH
LHTARTTGTPFARVAAQALCVPEDNIRSLLAPERMRKLGFTPQDLPQQCSGRTGHTVPDK
HHHHHCCCCHHHHHHHHHHCCCHHHHHHHHCHHHHHHCCCCCCHHHHHHCCCCCCCCCCC
ALPDESNNGE
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1711029; 8969508; 9384377 [H]