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The map label for this gene is rlmI [H]

Identifier: 78358490

GI number: 78358490

Start: 3412093

End: 3413283

Strand: Direct

Name: rlmI [H]

Synonym: Dde_3451

Alternate gene names: 78358490

Gene position: 3412093-3413283 (Clockwise)

Preceding gene: 78358489

Following gene: 78358491

Centisome position: 91.47

GC content: 59.45

Gene sequence:

>1191_bases
ATGAGACGACTGTACCTGAAGAAGGGCGAGGAGCGCCGCCTGCGCATCGGCCACCAATGGATTTTCAGCAACGAGGTGGA
CACAGGCAAAAGCCCTCTGCACGACTTCACCCCCGGAGAAAATGTAACCGTTTGCAACTCCGGCGGTTCTCCCGTTGCCT
CGGCGTATGTCAACCCGTCATCGGTCATTGCGGCCCGCGTTGTTTCGCAGCAGCCCGACACGCCGTTTGATGCCGGACTG
CTGCACAGACGCATAACGGAAGCTCTGGCCATGCGGCAGATGATTTTCGACAGACCATATTACCGCATGGTATTTGCCGA
AGGCGACTGGCTGCCCGGTCTCATCGTGGACCGGTACGGCGATTTTCTGGTGGCGCAGCTGACAACGGCAGGAGCCGAAG
CCGCCAAACCGCAGATAACCGCGGTACTGAAAGATGTGGTCAGACCGCGCGGACTCCTGTACCGCAACGACAGTGCCAAT
CGCGGAATGGAAGGTCTGAAAAGCTATGTGGAACAAGCCTTCGGACACATTCCCGACACGCTGGAAATAGAAGAAAACGG
AGTACGTTTTTACGCGCCGCTGATGGAAGGGCAGAAAACAGGCTGGTTTTATGACCAGCGCATCAACCGCAGAGACTTTG
CCTCGTTCGCACAGGGCAAAAAGGTGCTGGATGCATTCTGCTATGCAGGCGGGTTCGGTGTGACCGCAGCGGCTGCCGGT
GCCGCACAGACCACGTTTCTGGACGCCTCACCGCTTGCGCTGCAGACAGTGGAAGCCAACGCAGCCCTTAACCGCGTCAG
CGGCAGTGTAAAGACAGTGGCCGGAGACGCGCTGGAAACACTTGCCAGACTGCGCGATCAGGGCCGCCGCTACGATGTCA
TCTGTGTTGACCCGCCGGCGTTCATCAAACGTCGCAAGCACCAGAAGCAGGGGCTAAAGGCGTACCAGCGCGTAAACGAG
CTGGCCATGGAACTGGCAGAAGACGGCGGCATCCTGATGAGCTGCTCCTGTTCGCAGCATCTTTCCGCCGACGAGCTGCG
ACGCGTGCTCAACCGCGCCGCCCGCCGGGCAGGCTGCCGCATTCAGACGCTCACTCAGGGACATCAGGGCCCGGACCACC
CTGTTCATCCGGCCATGCCGGAAACGAATTATCTGAAAACATTCACCGTGCGGGTGAACCGCACAAGCTGA

Upstream 100 bases:

>100_bases
GACAACTGGGGTCAGGCGCACTAACAGGCCTGACGGCACAATAAAAAAGAGACCCGCAACACCCCGCGACCGCACCGCGG
CGCGAAGGACCCGACAGATA

Downstream 100 bases:

>100_bases
TTCAGTACCTACCTGAACCGGAGAATCTCATGCTGAACAAGCCCAGAATGCTCACCCCCGGCCCCACGCCCATACCCGCC
GCAGTGCGGCTGGCCATGGC

Product: SAM-dependent methyltransferase

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MRRLYLKKGEERRLRIGHQWIFSNEVDTGKSPLHDFTPGENVTVCNSGGSPVASAYVNPSSVIAARVVSQQPDTPFDAGL
LHRRITEALAMRQMIFDRPYYRMVFAEGDWLPGLIVDRYGDFLVAQLTTAGAEAAKPQITAVLKDVVRPRGLLYRNDSAN
RGMEGLKSYVEQAFGHIPDTLEIEENGVRFYAPLMEGQKTGWFYDQRINRRDFASFAQGKKVLDAFCYAGGFGVTAAAAG
AAQTTFLDASPLALQTVEANAALNRVSGSVKTVAGDALETLARLRDQGRRYDVICVDPPAFIKRRKHQKQGLKAYQRVNE
LAMELAEDGGILMSCSCSQHLSADELRRVLNRAARRAGCRIQTLTQGHQGPDHPVHPAMPETNYLKTFTVRVNRTS

Sequences:

>Translated_396_residues
MRRLYLKKGEERRLRIGHQWIFSNEVDTGKSPLHDFTPGENVTVCNSGGSPVASAYVNPSSVIAARVVSQQPDTPFDAGL
LHRRITEALAMRQMIFDRPYYRMVFAEGDWLPGLIVDRYGDFLVAQLTTAGAEAAKPQITAVLKDVVRPRGLLYRNDSAN
RGMEGLKSYVEQAFGHIPDTLEIEENGVRFYAPLMEGQKTGWFYDQRINRRDFASFAQGKKVLDAFCYAGGFGVTAAAAG
AAQTTFLDASPLALQTVEANAALNRVSGSVKTVAGDALETLARLRDQGRRYDVICVDPPAFIKRRKHQKQGLKAYQRVNE
LAMELAEDGGILMSCSCSQHLSADELRRVLNRAARRAGCRIQTLTQGHQGPDHPVHPAMPETNYLKTFTVRVNRTS
>Mature_396_residues
MRRLYLKKGEERRLRIGHQWIFSNEVDTGKSPLHDFTPGENVTVCNSGGSPVASAYVNPSSVIAARVVSQQPDTPFDAGL
LHRRITEALAMRQMIFDRPYYRMVFAEGDWLPGLIVDRYGDFLVAQLTTAGAEAAKPQITAVLKDVVRPRGLLYRNDSAN
RGMEGLKSYVEQAFGHIPDTLEIEENGVRFYAPLMEGQKTGWFYDQRINRRDFASFAQGKKVLDAFCYAGGFGVTAAAAG
AAQTTFLDASPLALQTVEANAALNRVSGSVKTVAGDALETLARLRDQGRRYDVICVDPPAFIKRRKHQKQGLKAYQRVNE
LAMELAEDGGILMSCSCSQHLSADELRRVLNRAARRAGCRIQTLTQGHQGPDHPVHPAMPETNYLKTFTVRVNRTS

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=397, Percent_Identity=34.5088161209068, Blast_Score=235, Evalue=4e-63,
Organism=Escherichia coli, GI1787181, Length=202, Percent_Identity=28.2178217821782, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 43890; Mature: 43890

Theoretical pI: Translated: 9.41; Mature: 9.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRLYLKKGEERRLRIGHQWIFSNEVDTGKSPLHDFTPGENVTVCNSGGSPVASAYVNPS
CCCCCCCCCCCHHHEECEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCCCCEEECCHH
SVIAARVVSQQPDTPFDAGLLHRRITEALAMRQMIFDRPYYRMVFAEGDWLPGLIVDRYG
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHCC
DFLVAQLTTAGAEAAKPQITAVLKDVVRPRGLLYRNDSANRGMEGLKSYVEQAFGHIPDT
CCEEHHHHHCCCHHCCHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCE
LEIEENGVRFYAPLMEGQKTGWFYDQRINRRDFASFAQGKKVLDAFCYAGGFGVTAAAAG
EEEECCCEEEEEECCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCC
AAQTTFLDASPLALQTVEANAALNRVSGSVKTVAGDALETLARLRDQGRRYDVICVDPPA
CCCEEEECCCCCEEEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCHH
FIKRRKHQKQGLKAYQRVNELAMELAEDGGILMSCSCSQHLSADELRRVLNRAARRAGCR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHCCCHHHHHHHHHHHHHHHCCE
IQTLTQGHQGPDHPVHPAMPETNYLKTFTVRVNRTS
EEEHHCCCCCCCCCCCCCCCCCCCEEEEEEEEECCC
>Mature Secondary Structure
MRRLYLKKGEERRLRIGHQWIFSNEVDTGKSPLHDFTPGENVTVCNSGGSPVASAYVNPS
CCCCCCCCCCCHHHEECEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCCCCEEECCHH
SVIAARVVSQQPDTPFDAGLLHRRITEALAMRQMIFDRPYYRMVFAEGDWLPGLIVDRYG
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHCC
DFLVAQLTTAGAEAAKPQITAVLKDVVRPRGLLYRNDSANRGMEGLKSYVEQAFGHIPDT
CCEEHHHHHCCCHHCCHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCE
LEIEENGVRFYAPLMEGQKTGWFYDQRINRRDFASFAQGKKVLDAFCYAGGFGVTAAAAG
EEEECCCEEEEEECCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCC
AAQTTFLDASPLALQTVEANAALNRVSGSVKTVAGDALETLARLRDQGRRYDVICVDPPA
CCCEEEECCCCCEEEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCHH
FIKRRKHQKQGLKAYQRVNELAMELAEDGGILMSCSCSQHLSADELRRVLNRAARRAGCR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHCCCHHHHHHHHHHHHHHHCCE
IQTLTQGHQGPDHPVHPAMPETNYLKTFTVRVNRTS
EEEHHCCCCCCCCCCCCCCCCCCCEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA