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The map label for this gene is leuA [H]

Identifier: 78358258

GI number: 78358258

Start: 3207778

End: 3209301

Strand: Direct

Name: leuA [H]

Synonym: Dde_3218

Alternate gene names: 78358258

Gene position: 3207778-3209301 (Clockwise)

Preceding gene: 78358257

Following gene: 78358259

Centisome position: 85.99

GC content: 58.79

Gene sequence:

>1524_bases
ATGGCTGACAGATTGTACATTTTCGACACCACGTTGCGCGACGGCGAACAGTCGCCCGGCGCCACCATGAACATTCATGA
AAAGGTGCAGCTGGCCCGCCAGCTGGAAATGGTCGGTGTGGACATTATGGAGGCCGGCTTTCCGGCTGCCAGCAAAGGCG
ACTTTGAAGCTGTGAAAACCATAGCCGCCACCGTGAAAAATGCACAGGTCGCCGGTCTGTGCCGCGCCGTGCAGCCCGAC
ATCGACCACGCATGGGCAGCCATCAGAAACGCAGCACATCCGCGTATTCACACCTTTATCGCCACCTCGCCGGTGCATAT
GCAGTACAAACTGCGCAAATCGCCCGAACAGGTGCTGGAAATGGCGCGCGACGCAGTGCGGTATGCCGCAGGCCTGTGCT
CCAATGTGGAATTCAGCGCCGAAGACGCATCGCGTTCCGACTGGGATTTTCTTACCCGCGTTGTGGAAACCGCCATTGAC
GCCGGTGCCACCACCATCAACATTCCCGACACCGTGGGCTATGCACAACCGGACGAATTCGGCGCACTGGTGCGTTATGT
CATGGAAAACGCGCGCAACAGCCACAAGGCCGTCTTTTCCGTCCACTGCCACAACGACCTCGGTCTGGCGGTGGCCAACA
CGCTGGCCGCGCTCAAAGCAGGTGCACGGCAGGCAGAGGTCACCGTATCAGGCATAGGCGAACGTGCCGGCAACGCCGCG
CTGGAAGAACTTGTCATGGCCCTGCGGACACGGCATGCTTACTACGACATGGAAACAGGAATAAAGTCAGAGCAGCTTTT
CCCTGCCTGCCGCCTGCTTTCCATGATCATCGGGCAGCCCATACCCGCCAACAAAGCCATTGTAGGGGCCAACGCCTTTG
CGCACGAGTCAGGCATCCATCAGGACGGCATGCTGAAAAACCGCGAGACATACGAAATAATGACGCCGGAATCCGTGGGT
CGCACCAAGACGGAAATCGTTCTGGGCAAGCACTCGGGCCGCAACGCCATCAACGGCAAGCTTGAAGAGCTCGGCTACCG
CCTGACTCCGGAACAGACCCAGCTGGTGTTCGATGCCGTAAAGAAGCTCGCCGACAGGAAAAAACAGATCTACGACGAAG
ATATGGAAGCGCTGGTGCTTGAAGAGGTGTTCCGCATACCCGACATGTTCCGGCTTGTGCACCTCAGCGTGCAGGCCAGC
GATACGGGAGTTCCCCCTTCCGCCGCCATTGTCATGGAAGTGGGCGGCGAACGCCGCCAGCACACCACCTTCGGCGTGGG
TCCCATTGACGCGGTATTCAATGCCATATCACAGATAGTGGGACGTGCCCCGCAGCTGCGGCATTATTCGGTCAACGCCA
TCACCGGCGGCACCGACGCGCAGGGCGAGGTAACAGTCAAGCTGCAGGAAAACGGCAGTGCCGCGGTAGGCCGCGGTGCC
GATGCTGATATCATCACAGCCAGTGCAAAAGCGTATCTCAATGCGCTGAACAGGCTTGCCAAAAAAGAACAGGAGGACAA
ATAG

Upstream 100 bases:

>100_bases
ATCATAAGCCCGCAGGCCACGTGATGCAGGGAAGACGAACCCGACTCCTCCAAGCCCTGAAATAGCGGCACATAAAGCCG
CATAGCCGTAGGAGTATCCC

Downstream 100 bases:

>100_bases
ATGGCTCATACGCTTGCGCAGAAGATACTGCAGAAGCACACAGACCAGCAGGTGGGCGAAGCAGGGCAGATAGTGCGCTG
CCGCGTTTCCATGGCGCTGG

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 507; Mature: 506

Protein sequence:

>507_residues
MADRLYIFDTTLRDGEQSPGATMNIHEKVQLARQLEMVGVDIMEAGFPAASKGDFEAVKTIAATVKNAQVAGLCRAVQPD
IDHAWAAIRNAAHPRIHTFIATSPVHMQYKLRKSPEQVLEMARDAVRYAAGLCSNVEFSAEDASRSDWDFLTRVVETAID
AGATTINIPDTVGYAQPDEFGALVRYVMENARNSHKAVFSVHCHNDLGLAVANTLAALKAGARQAEVTVSGIGERAGNAA
LEELVMALRTRHAYYDMETGIKSEQLFPACRLLSMIIGQPIPANKAIVGANAFAHESGIHQDGMLKNRETYEIMTPESVG
RTKTEIVLGKHSGRNAINGKLEELGYRLTPEQTQLVFDAVKKLADRKKQIYDEDMEALVLEEVFRIPDMFRLVHLSVQAS
DTGVPPSAAIVMEVGGERRQHTTFGVGPIDAVFNAISQIVGRAPQLRHYSVNAITGGTDAQGEVTVKLQENGSAAVGRGA
DADIITASAKAYLNALNRLAKKEQEDK

Sequences:

>Translated_507_residues
MADRLYIFDTTLRDGEQSPGATMNIHEKVQLARQLEMVGVDIMEAGFPAASKGDFEAVKTIAATVKNAQVAGLCRAVQPD
IDHAWAAIRNAAHPRIHTFIATSPVHMQYKLRKSPEQVLEMARDAVRYAAGLCSNVEFSAEDASRSDWDFLTRVVETAID
AGATTINIPDTVGYAQPDEFGALVRYVMENARNSHKAVFSVHCHNDLGLAVANTLAALKAGARQAEVTVSGIGERAGNAA
LEELVMALRTRHAYYDMETGIKSEQLFPACRLLSMIIGQPIPANKAIVGANAFAHESGIHQDGMLKNRETYEIMTPESVG
RTKTEIVLGKHSGRNAINGKLEELGYRLTPEQTQLVFDAVKKLADRKKQIYDEDMEALVLEEVFRIPDMFRLVHLSVQAS
DTGVPPSAAIVMEVGGERRQHTTFGVGPIDAVFNAISQIVGRAPQLRHYSVNAITGGTDAQGEVTVKLQENGSAAVGRGA
DADIITASAKAYLNALNRLAKKEQEDK
>Mature_506_residues
ADRLYIFDTTLRDGEQSPGATMNIHEKVQLARQLEMVGVDIMEAGFPAASKGDFEAVKTIAATVKNAQVAGLCRAVQPDI
DHAWAAIRNAAHPRIHTFIATSPVHMQYKLRKSPEQVLEMARDAVRYAAGLCSNVEFSAEDASRSDWDFLTRVVETAIDA
GATTINIPDTVGYAQPDEFGALVRYVMENARNSHKAVFSVHCHNDLGLAVANTLAALKAGARQAEVTVSGIGERAGNAAL
EELVMALRTRHAYYDMETGIKSEQLFPACRLLSMIIGQPIPANKAIVGANAFAHESGIHQDGMLKNRETYEIMTPESVGR
TKTEIVLGKHSGRNAINGKLEELGYRLTPEQTQLVFDAVKKLADRKKQIYDEDMEALVLEEVFRIPDMFRLVHLSVQASD
TGVPPSAAIVMEVGGERRQHTTFGVGPIDAVFNAISQIVGRAPQLRHYSVNAITGGTDAQGEVTVKLQENGSAAVGRGAD
ADIITASAKAYLNALNRLAKKEQEDK

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=512, Percent_Identity=47.265625, Blast_Score=457, Evalue=1e-130,
Organism=Saccharomyces cerevisiae, GI6320019, Length=377, Percent_Identity=31.0344827586207, Blast_Score=157, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6320071, Length=373, Percent_Identity=31.3672922252011, Blast_Score=157, Evalue=4e-39,
Organism=Saccharomyces cerevisiae, GI6324225, Length=533, Percent_Identity=24.015009380863, Blast_Score=116, Evalue=7e-27,
Organism=Saccharomyces cerevisiae, GI6324682, Length=545, Percent_Identity=24.5871559633028, Blast_Score=115, Evalue=1e-26,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 55058; Mature: 54927

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00104 EPSP_SYNTHASE_1 ; PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADRLYIFDTTLRDGEQSPGATMNIHEKVQLARQLEMVGVDIMEAGFPAASKGDFEAVKT
CCCEEEEEECCCCCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
IAATVKNAQVAGLCRAVQPDIDHAWAAIRNAAHPRIHTFIATSPVHMQYKLRKSPEQVLE
HHHHHHCHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEEECCCEEEEEECCCCHHHHHH
MARDAVRYAAGLCSNVEFSAEDASRSDWDFLTRVVETAIDAGATTINIPDTVGYAQPDEF
HHHHHHHHHHHHHCCCEECCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHH
GALVRYVMENARNSHKAVFSVHCHNDLGLAVANTLAALKAGARQAEVTVSGIGERAGNAA
HHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHH
LEELVMALRTRHAYYDMETGIKSEQLFPACRLLSMIIGQPIPANKAIVGANAFAHESGIH
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCEEECCCHHHHHCCCC
QDGMLKNRETYEIMTPESVGRTKTEIVLGKHSGRNAINGKLEELGYRLTPEQTQLVFDAV
CCCCCCCCCCEEEECCHHCCCCEEEEEEECCCCCCCCCCCHHHCCCCCCCHHHHHHHHHH
KKLADRKKQIYDEDMEALVLEEVFRIPDMFRLVHLSVQASDTGVPPSAAIVMEVGGERRQ
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHEEEECCCCCCCCCCEEEEECCCCCCC
HTTFGVGPIDAVFNAISQIVGRAPQLRHYSVNAITGGTDAQGEVTVKLQENGSAAVGRGA
CCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCEEEEEEECCCCEECCCCC
DADIITASAKAYLNALNRLAKKEQEDK
CCCEEEHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ADRLYIFDTTLRDGEQSPGATMNIHEKVQLARQLEMVGVDIMEAGFPAASKGDFEAVKT
CCEEEEEECCCCCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
IAATVKNAQVAGLCRAVQPDIDHAWAAIRNAAHPRIHTFIATSPVHMQYKLRKSPEQVLE
HHHHHHCHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEEECCCEEEEEECCCCHHHHHH
MARDAVRYAAGLCSNVEFSAEDASRSDWDFLTRVVETAIDAGATTINIPDTVGYAQPDEF
HHHHHHHHHHHHHCCCEECCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHH
GALVRYVMENARNSHKAVFSVHCHNDLGLAVANTLAALKAGARQAEVTVSGIGERAGNAA
HHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHH
LEELVMALRTRHAYYDMETGIKSEQLFPACRLLSMIIGQPIPANKAIVGANAFAHESGIH
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCEEECCCHHHHHCCCC
QDGMLKNRETYEIMTPESVGRTKTEIVLGKHSGRNAINGKLEELGYRLTPEQTQLVFDAV
CCCCCCCCCCEEEECCHHCCCCEEEEEEECCCCCCCCCCCHHHCCCCCCCHHHHHHHHHH
KKLADRKKQIYDEDMEALVLEEVFRIPDMFRLVHLSVQASDTGVPPSAAIVMEVGGERRQ
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHEEEECCCCCCCCCCEEEEECCCCCCC
HTTFGVGPIDAVFNAISQIVGRAPQLRHYSVNAITGGTDAQGEVTVKLQENGSAAVGRGA
CCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCEEEEEEECCCCEECCCCC
DADIITASAKAYLNALNRLAKKEQEDK
CCCEEEHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA