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The map label for this gene is dosC [H]

Identifier: 78358230

GI number: 78358230

Start: 3177669

End: 3180227

Strand: Direct

Name: dosC [H]

Synonym: Dde_3190

Alternate gene names: 78358230

Gene position: 3177669-3180227 (Clockwise)

Preceding gene: 78358229

Following gene: 78358231

Centisome position: 85.19

GC content: 59.55

Gene sequence:

>2559_bases
ATGACCACGCAAGAAGCAGCTGACCGCAGTAAGACCATGGAGTGGTACTCCGAGTGTCTTCAGCTCACGTTTCCCCCTGT
GATGGTCAAACTGCTGCAGGCGCTTGCCCAGCCGGAGCCCGATTTTTCTGTCATCGCCGAGCTGCTGGCCATGGACCCGG
TTCTTGCGGCCACCATTCTGGAACTGGTCAACTCTCCCTACTACGGGCAGCGCAATCCCGTGTCCGACATGCAGCGGGCC
GTAGTGGTACTCGGCTCGCGGGAAATTCTCAAAATAGCCCTGTCCATAGCCTACCAGCAGCGCGTGACCGGCGCACTGCA
CCGCAAACCAACCGAACTTTTTCCCGACTGGCGCATGGCACTGTGGAGCGCCATTGCCGCAGAAAAACTGTCGGCCCAGC
TGGCACCCGGCGACGAGGCTCTGGCCTATCTTGCCGCACTGCTCAAAGATCTGGGAATTTTTATCAGACAGTGCACCCGC
AGTTCAGACCCCGTGCTTGCCCGCCGCCGTTATCTGACGGAATATTCCCGCGCCCACGCCACATCCGAACAGGTCCACTG
GGGCAAAGAGCATCAGGAGCTTTCCGCAGAACTGTTCAGCACATGGAACATGCCGCAACGCTTTGTCGACGCGGTGCGCC
ACCATCATGCCCCGACGCTTAACGGGCTGGACCCCTTCACGTTGTGCGTCGCGCTTGCCACCCGCTGGGCCGAACTGCTG
CAGACCGGACAGGGCGATCATGCGGAACTCATCCGCCATGAAATGCTGATGGCCGGGCTGCTGCAGCTTGACGGGCCGGG
TATGGAAGCGCTGCGCGCCGACTGTGCCACCCGCTTCCGCGTTCTGCTCAGCTCTCTGGGCATAGAAGACGCCCCGCTTT
CCGACAGACTCTACACCCAGACGCTTGAAGACATTCAGCGCTTTCATTTTCTGAGCACAGAACTGCAGCGCGCCGCCTCG
TACGAAGAAGCCGTCAAAATGACCGGCAGACAGCTGCACTGGCTGTGGAACATGAAAAAATGGGAAGTGGCTCTGCGCGA
TCCTTCCGTAAACAGATATGTGCTTTTCCGTCCTGTGCCCGAACAGGGTGTGCAGCCCGTTGCCAGCAGTGCCGAGGTTG
ACGCCCTGCCGTGGAGCAGAGGCGGTGTGCGCCTGCCGCTGGCTGCCGAGGGTGACGTGTACGGCAGCATGAACGTGCCG
CGCAGCAAAACCAGACTGCAGGAAGAAGGCAGCCTGGCCGTATACGCCAGATTTCTCTCAAAAGGGCTGGAAATATTCTA
TACGGAACAGAGCGGACTGCGCAGTAAAGCCCGTCTGCTTGATTCGCTGCCCAACATGGTGGCGCAGGTGGACGCCACAG
GCATCATACGCGAAGCCAACACGGCCTTTCTTGCACTTGCCGGGCAGGACAGCGACCCGCGCGGGCATGCTTTAAGCGAC
GTGCTGCGCAATGCATACGGCATTGAGACCGAATCAGTATGGCGGCAGATCACGGCGGAAACACAAAACAATGCCGCAAA
CAAAATTTTTACATACTGCACCACGCAGGGAGCAGCCCGTACCTGCGCTCTGCTCAGCATCTTCCGCAACCTGCCTGAGC
AGGGGGCATTTCTGGTACGTATCGAAGACATAACACAGGTTTCCGCACTGGAAGCCGAAGTGCTGGAACAAAATGACTTT
CTGAGCAGCCTCATGGAGGCCATGCAGGAGGTGGTGTTCACCGTCGACGCCACCGGCAGAGTGCTGTGGACCAGCTCGGA
CATGCTGCTGCAGGCCGGTGCCATGCTGTTTGACATCGCCGCCGCCACAACGCCCGGTGCCGCGTGGGGGGCCGATCATC
TGCCTGTGCAGCGGCAGGCGGACACTGCGGAACAGCAGACGGCACACCCGCAGGAAAAACAGACGCCCGTACCGGTGGAA
GTCGCCATAAAACTGCCCGACGGCAGCAGCGCACTGTACGAGCTTTCAGTCTCGCCGCTCAAAACGCGGGCGGAAACAGC
GCGCTGGATCATCGTGGGGCGCGATCTGACCAGCATCCGGCGCATGGAAGAAAAAATGCGCATGCAGGCAGTGTTTGACG
GCCTCACAGGGCTTTTCAATCATACGCATTTTCACATGCTGCTCGACAGAGAAATAGCCCGCTGCCATCGCACACGGCAT
CCTCTGGCACTTATCTTCCTTGATCTGGACGGGTTCAAAGCCATCAACGACACGCAAGGGCACCGCGAAGGCGACAGAAT
ACTGCGTCAGGTTTCCAACGCCCTGCGCTCCTGCATACGCAAAGGAACTGATTTTGCCTGCCGCTACGGCGGCGACGAAT
TTACCGTCATCGTGACGGAATTCAAAGAAGACGCGCTACCCGCCATGTCCGCCCGCATACTGGACGCCATTGAATCGGGC
ACATCAGGTGCTGTAAGCGCCAGTGTGGGGGTTGCCCTTCTGCAATCGCAGGATTCTGCGGAAAGTCTTGTCCAGCGGGC
CGACACCGCAAGCTACGCCGCAAAAAAAGCCGGAGGACGGCAGGTTGCTCAAGCCCCCCTGCATCCCGGTTCCGCATGA

Upstream 100 bases:

>100_bases
AAGCCGCAGACGCACTGCACATGCTGGACGAAAGCAGGGAAAAAACCGTGCTCAGGCAAACGGTGGATTATGTGAAAAAC
CGCAGCAGGTAGGAAGGCCC

Downstream 100 bases:

>100_bases
CAGAACACAGCGCCGCCCACCCCCCGCACGGGGCTGCGCCTCTTTTTTTATCCGCAGGGCCGCAGTGCCCCGCGGCGCAA
CATTCTGTTTTTACCCTTTG

Product: diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 852; Mature: 851

Protein sequence:

>852_residues
MTTQEAADRSKTMEWYSECLQLTFPPVMVKLLQALAQPEPDFSVIAELLAMDPVLAATILELVNSPYYGQRNPVSDMQRA
VVVLGSREILKIALSIAYQQRVTGALHRKPTELFPDWRMALWSAIAAEKLSAQLAPGDEALAYLAALLKDLGIFIRQCTR
SSDPVLARRRYLTEYSRAHATSEQVHWGKEHQELSAELFSTWNMPQRFVDAVRHHHAPTLNGLDPFTLCVALATRWAELL
QTGQGDHAELIRHEMLMAGLLQLDGPGMEALRADCATRFRVLLSSLGIEDAPLSDRLYTQTLEDIQRFHFLSTELQRAAS
YEEAVKMTGRQLHWLWNMKKWEVALRDPSVNRYVLFRPVPEQGVQPVASSAEVDALPWSRGGVRLPLAAEGDVYGSMNVP
RSKTRLQEEGSLAVYARFLSKGLEIFYTEQSGLRSKARLLDSLPNMVAQVDATGIIREANTAFLALAGQDSDPRGHALSD
VLRNAYGIETESVWRQITAETQNNAANKIFTYCTTQGAARTCALLSIFRNLPEQGAFLVRIEDITQVSALEAEVLEQNDF
LSSLMEAMQEVVFTVDATGRVLWTSSDMLLQAGAMLFDIAAATTPGAAWGADHLPVQRQADTAEQQTAHPQEKQTPVPVE
VAIKLPDGSSALYELSVSPLKTRAETARWIIVGRDLTSIRRMEEKMRMQAVFDGLTGLFNHTHFHMLLDREIARCHRTRH
PLALIFLDLDGFKAINDTQGHREGDRILRQVSNALRSCIRKGTDFACRYGGDEFTVIVTEFKEDALPAMSARILDAIESG
TSGAVSASVGVALLQSQDSAESLVQRADTASYAAKKAGGRQVAQAPLHPGSA

Sequences:

>Translated_852_residues
MTTQEAADRSKTMEWYSECLQLTFPPVMVKLLQALAQPEPDFSVIAELLAMDPVLAATILELVNSPYYGQRNPVSDMQRA
VVVLGSREILKIALSIAYQQRVTGALHRKPTELFPDWRMALWSAIAAEKLSAQLAPGDEALAYLAALLKDLGIFIRQCTR
SSDPVLARRRYLTEYSRAHATSEQVHWGKEHQELSAELFSTWNMPQRFVDAVRHHHAPTLNGLDPFTLCVALATRWAELL
QTGQGDHAELIRHEMLMAGLLQLDGPGMEALRADCATRFRVLLSSLGIEDAPLSDRLYTQTLEDIQRFHFLSTELQRAAS
YEEAVKMTGRQLHWLWNMKKWEVALRDPSVNRYVLFRPVPEQGVQPVASSAEVDALPWSRGGVRLPLAAEGDVYGSMNVP
RSKTRLQEEGSLAVYARFLSKGLEIFYTEQSGLRSKARLLDSLPNMVAQVDATGIIREANTAFLALAGQDSDPRGHALSD
VLRNAYGIETESVWRQITAETQNNAANKIFTYCTTQGAARTCALLSIFRNLPEQGAFLVRIEDITQVSALEAEVLEQNDF
LSSLMEAMQEVVFTVDATGRVLWTSSDMLLQAGAMLFDIAAATTPGAAWGADHLPVQRQADTAEQQTAHPQEKQTPVPVE
VAIKLPDGSSALYELSVSPLKTRAETARWIIVGRDLTSIRRMEEKMRMQAVFDGLTGLFNHTHFHMLLDREIARCHRTRH
PLALIFLDLDGFKAINDTQGHREGDRILRQVSNALRSCIRKGTDFACRYGGDEFTVIVTEFKEDALPAMSARILDAIESG
TSGAVSASVGVALLQSQDSAESLVQRADTASYAAKKAGGRQVAQAPLHPGSA
>Mature_851_residues
TTQEAADRSKTMEWYSECLQLTFPPVMVKLLQALAQPEPDFSVIAELLAMDPVLAATILELVNSPYYGQRNPVSDMQRAV
VVLGSREILKIALSIAYQQRVTGALHRKPTELFPDWRMALWSAIAAEKLSAQLAPGDEALAYLAALLKDLGIFIRQCTRS
SDPVLARRRYLTEYSRAHATSEQVHWGKEHQELSAELFSTWNMPQRFVDAVRHHHAPTLNGLDPFTLCVALATRWAELLQ
TGQGDHAELIRHEMLMAGLLQLDGPGMEALRADCATRFRVLLSSLGIEDAPLSDRLYTQTLEDIQRFHFLSTELQRAASY
EEAVKMTGRQLHWLWNMKKWEVALRDPSVNRYVLFRPVPEQGVQPVASSAEVDALPWSRGGVRLPLAAEGDVYGSMNVPR
SKTRLQEEGSLAVYARFLSKGLEIFYTEQSGLRSKARLLDSLPNMVAQVDATGIIREANTAFLALAGQDSDPRGHALSDV
LRNAYGIETESVWRQITAETQNNAANKIFTYCTTQGAARTCALLSIFRNLPEQGAFLVRIEDITQVSALEAEVLEQNDFL
SSLMEAMQEVVFTVDATGRVLWTSSDMLLQAGAMLFDIAAATTPGAAWGADHLPVQRQADTAEQQTAHPQEKQTPVPVEV
AIKLPDGSSALYELSVSPLKTRAETARWIIVGRDLTSIRRMEEKMRMQAVFDGLTGLFNHTHFHMLLDREIARCHRTRHP
LALIFLDLDGFKAINDTQGHREGDRILRQVSNALRSCIRKGTDFACRYGGDEFTVIVTEFKEDALPAMSARILDAIESGT
SGAVSASVGVALLQSQDSAESLVQRADTASYAAKKAGGRQVAQAPLHPGSA

Specific function: Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=190, Percent_Identity=37.3684210526316, Blast_Score=118, Evalue=1e-27,
Organism=Escherichia coli, GI1787541, Length=178, Percent_Identity=38.2022471910112, Blast_Score=107, Evalue=4e-24,
Organism=Escherichia coli, GI1788381, Length=324, Percent_Identity=25.9259259259259, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI87081881, Length=211, Percent_Identity=30.8056872037915, Blast_Score=100, Evalue=6e-22,
Organism=Escherichia coli, GI1786584, Length=171, Percent_Identity=37.4269005847953, Blast_Score=94, Evalue=5e-20,
Organism=Escherichia coli, GI87082007, Length=190, Percent_Identity=34.7368421052632, Blast_Score=81, Evalue=3e-16,
Organism=Escherichia coli, GI1787262, Length=160, Percent_Identity=31.875, Blast_Score=80, Evalue=6e-16,
Organism=Escherichia coli, GI87081974, Length=177, Percent_Identity=27.1186440677966, Blast_Score=77, Evalue=5e-15,
Organism=Escherichia coli, GI87081977, Length=228, Percent_Identity=30.2631578947368, Blast_Score=66, Evalue=9e-12,
Organism=Escherichia coli, GI1788956, Length=173, Percent_Identity=31.7919075144509, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1787816, Length=123, Percent_Identity=32.520325203252, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR012292
- InterPro:   IPR009050 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 94304; Mature: 94172

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS50113 PAC ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTQEAADRSKTMEWYSECLQLTFPPVMVKLLQALAQPEPDFSVIAELLAMDPVLAATIL
CCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCHHHHHHHH
ELVNSPYYGQRNPVSDMQRAVVVLGSREILKIALSIAYQQRVTGALHRKPTELFPDWRMA
HHHCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHCCHHHHH
LWSAIAAEKLSAQLAPGDEALAYLAALLKDLGIFIRQCTRSSDPVLARRRYLTEYSRAHA
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHC
TSEQVHWGKEHQELSAELFSTWNMPQRFVDAVRHHHAPTLNGLDPFTLCVALATRWAELL
CHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
QTGQGDHAELIRHEMLMAGLLQLDGPGMEALRADCATRFRVLLSSLGIEDAPLSDRLYTQ
HCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
TLEDIQRFHFLSTELQRAASYEEAVKMTGRQLHWLWNMKKWEVALRDPSVNRYVLFRPVP
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEEECCCCCEEEEEECCC
EQGVQPVASSAEVDALPWSRGGVRLPLAAEGDVYGSMNVPRSKTRLQEEGSLAVYARFLS
HHCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHH
KGLEIFYTEQSGLRSKARLLDSLPNMVAQVDATGIIREANTAFLALAGQDSDPRGHALSD
CCCEEEEECCCCHHHHHHHHHHHHHHHHHHCHHHHEEECCCEEEEEECCCCCCCCHHHHH
VLRNAYGIETESVWRQITAETQNNAANKIFTYCTTQGAARTCALLSIFRNLPEQGAFLVR
HHHHHCCCCHHHHHHHHHHHHCCCCHHHEEEEECCCCHHHHHHHHHHHHCCCCCCEEEEE
IEDITQVSALEAEVLEQNDFLSSLMEAMQEVVFTVDATGRVLWTSSDMLLQAGAMLFDIA
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEECCHHHHHHHHHHHHHH
AATTPGAAWGADHLPVQRQADTAEQQTAHPQEKQTPVPVEVAIKLPDGSSALYELSVSPL
HCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCEEEEEECCCCCCCEEEEECCHH
KTRAETARWIIVGRDLTSIRRMEEKMRMQAVFDGLTGLFNHTHFHMLLDREIARCHRTRH
HHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCC
PLALIFLDLDGFKAINDTQGHREGDRILRQVSNALRSCIRKGTDFACRYGGDEFTVIVTE
CEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEEEE
FKEDALPAMSARILDAIESGTSGAVSASVGVALLQSQDSAESLVQRADTASYAAKKAGGR
CCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCC
QVAQAPLHPGSA
HHHHCCCCCCCC
>Mature Secondary Structure 
TTQEAADRSKTMEWYSECLQLTFPPVMVKLLQALAQPEPDFSVIAELLAMDPVLAATIL
CCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCHHHHHHHH
ELVNSPYYGQRNPVSDMQRAVVVLGSREILKIALSIAYQQRVTGALHRKPTELFPDWRMA
HHHCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHCCHHHHH
LWSAIAAEKLSAQLAPGDEALAYLAALLKDLGIFIRQCTRSSDPVLARRRYLTEYSRAHA
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHC
TSEQVHWGKEHQELSAELFSTWNMPQRFVDAVRHHHAPTLNGLDPFTLCVALATRWAELL
CHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
QTGQGDHAELIRHEMLMAGLLQLDGPGMEALRADCATRFRVLLSSLGIEDAPLSDRLYTQ
HCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
TLEDIQRFHFLSTELQRAASYEEAVKMTGRQLHWLWNMKKWEVALRDPSVNRYVLFRPVP
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEEECCCCCEEEEEECCC
EQGVQPVASSAEVDALPWSRGGVRLPLAAEGDVYGSMNVPRSKTRLQEEGSLAVYARFLS
HHCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHH
KGLEIFYTEQSGLRSKARLLDSLPNMVAQVDATGIIREANTAFLALAGQDSDPRGHALSD
CCCEEEEECCCCHHHHHHHHHHHHHHHHHHCHHHHEEECCCEEEEEECCCCCCCCHHHHH
VLRNAYGIETESVWRQITAETQNNAANKIFTYCTTQGAARTCALLSIFRNLPEQGAFLVR
HHHHHCCCCHHHHHHHHHHHHCCCCHHHEEEEECCCCHHHHHHHHHHHHCCCCCCEEEEE
IEDITQVSALEAEVLEQNDFLSSLMEAMQEVVFTVDATGRVLWTSSDMLLQAGAMLFDIA
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEECCHHHHHHHHHHHHHH
AATTPGAAWGADHLPVQRQADTAEQQTAHPQEKQTPVPVEVAIKLPDGSSALYELSVSPL
HCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCEEEEEECCCCCCCEEEEECCHH
KTRAETARWIIVGRDLTSIRRMEEKMRMQAVFDGLTGLFNHTHFHMLLDREIARCHRTRH
HHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCC
PLALIFLDLDGFKAINDTQGHREGDRILRQVSNALRSCIRKGTDFACRYGGDEFTVIVTE
CEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEEEE
FKEDALPAMSARILDAIESGTSGAVSASVGVALLQSQDSAESLVQRADTASYAAKKAGGR
CCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCC
QVAQAPLHPGSA
HHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]