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The map label for this gene is cbiH [H]

Identifier: 78358221

GI number: 78358221

Start: 3167659

End: 3169626

Strand: Direct

Name: cbiH [H]

Synonym: Dde_3181

Alternate gene names: 78358221

Gene position: 3167659-3169626 (Clockwise)

Preceding gene: 78358220

Following gene: 78358222

Centisome position: 84.92

GC content: 61.79

Gene sequence:

>1968_bases
ATGCTGCCGGAGCGCCTGAACGGTGCTCCGGCTTTTTTCACCTTTGCGCACGGCAACATCATGAACAGCACACCCCGTTC
CCTGCACGACGTCTCCGGCACAGATTCCGGCGGCACACATACGTCACGGCTGTGTGTTTTTGCACTGACTCCTCAGGGAA
TACAGCTGGCCGCGCGGCTCTGCCCGCATCTGCAGGCAGACCTGTTTCTGCCGCACCGGCTTGCCGGTCTTGCAGACAGC
ACAACCGCCCCCCGCACAGAGCGTTTTCTCCGGCTTGCCGATGCCGTGCGGCATGCGTTTTCAATTTATGACGGTCTGAT
TTTTATCGCGGCATGCGGCATAGCCGTGCGCAGCATAGCGCCGCTGCTGCAGGGCAAAGACCGCGACCCCGCCGTGGTGG
TGCTGGACCAGCAGGGGCTCCACGCCGTCAGCCTGCTGTCGGGACATCTCGGCGGAGCAAACGCCCTTGCCTGCCGCATA
GCCGCCATGACCGGCGGCACACCTGTCATCACCACCGCCACTGATACCGAAGGACTGCCGTCACTCGATCTGCTGGCGGC
GGAAAACAACATGGCCATAGCCCGGCTGACCGCAGTACGGCACGTCAACGCCGCACTGCTGGAAGGGCGGCAGGTGGCCG
TTGCCGACCGCCACGACCTGCTGAAGCTGAAAAATCACCCCTGCGGCCTGTTCATTCACACCGACGAACACAGTCTGGAC
AACCGGTACGCGGCACTACCCTCCGTACTGGTGACAATCCATGCATCGGCTGCCCGGCGCGCTCCTGCAGACAGGCTGGT
ACTGCATCCGCGCATTCTTCATGCAGGTCTGGGCTGCCGCAAAAACGTGCCCGCGCCAAGAGCGGCTGAACACCTGCTGC
GGCTGCTGGAAGCGCATGACATCACTCCGCTGGCACTGGCATCGCTGGCAAGCGTGGAAGCTAAACGGGCTGAACCGGCC
ATGCACGAGGCCTCCCGCAGGCTGGGGGTGCCCGTTATCTTTTTTTCCTCTGCCGCACTGGCCGCAATTGCGGTACCCAA
CCCGTCGTCCAGACCGCTGAAAGCCGTGGGCACCCCTTCCGTGGCGGAGGCTGCCGCCCTGCTGGCAGCGCGGACAAACA
ATGAATGCCGCTGCACACAGCATGCCGCGGCACATCTTATCATACCCAAAGCAGCCGACGGCGACGTGACCGCTGCCGTG
GCACAGGAGCAATACACCATGCACCCCGCACCTCTTTACATCGTCGGTCTGGGCCCCGGAGACCCGCAGCTGCTCACCCC
GCAGGCTGCGGACGCATTGCGGGCTTCAGAAGTCATAGCAGGGTACACCCGCTATGTTGAACTGGTGCCGGACGATATTC
TTGCCGGACGCGAAATCATTGCCACGGGCATGATGGGCGAAATGGAACGCTGCGCCAAGGCCATTGAAACTGCTCTTTCC
GGCAGGGCCACGGCAGTGGTGTGCTCCGGCGATCCCGGCATTTATGCCATGGCCGGACTTATCTATGAACTGATCGAAGA
AATGGACGCCTATCTGCCCGTGCACGTCATCGCCGGAGTGCCTGCACTTTCTGCGGCTGCGGCGCTGCTGGGCGCACCGC
TGATGCACGATTTCGCCTGCATCAGCCTGAGTGACCTGCTCACCCCCTGGGAAGTTATCGAAAAGCGCCTGCAAAACGCA
CTGGAAGCTGATTTCGTTGTGGTGCTGTACAACCCGCGCTCAAAAAAACGCGACTGGCAGCTGGAGCATGCGCTGGAAAC
GGCCCGCCGCTACAGACAGCCCGAAACCCCTGTCGGACTGGTACGGCAGGCCAACAGGCCGGAACAGGCTGTTTCGGTTG
CCCCTCTGTCGGTTTTCGAACCGGCGCAGGTGGACATGCTCTCCATTGTGATCATAGGCAACAGCACCACCAGAATAATA
CGGGGCAACATGGTGACACCCCGCGGATACATGAAAAAATACCGCTGA

Upstream 100 bases:

>100_bases
CCCACACAGAAGACGACTTTGCGGCCATGCTCGCAGCAGTGCGCAACGTCACCTTCTAGGGCAAATCCTGCGGGTATGGT
CCCGCCCCCGCAACAATGCT

Downstream 100 bases:

>100_bases
GCCGGACACGGCGCAGGCATCTGCGCTTTGCACGCCGCGCAATGCAGCCTGCGCGGCCCGCCGCAGGTACGCGCAGGCTG
CCCCCCCGCGGGCACAGCGC

Product: precorrin-3 methyltransferase

Products: S-adenosyl-L-homocysteine; Precorrin 4

Alternate protein names: Cobalt-precorrin-3 methylase; Cobalt-precorrin-3 methyltransferase [H]

Number of amino acids: Translated: 655; Mature: 655

Protein sequence:

>655_residues
MLPERLNGAPAFFTFAHGNIMNSTPRSLHDVSGTDSGGTHTSRLCVFALTPQGIQLAARLCPHLQADLFLPHRLAGLADS
TTAPRTERFLRLADAVRHAFSIYDGLIFIAACGIAVRSIAPLLQGKDRDPAVVVLDQQGLHAVSLLSGHLGGANALACRI
AAMTGGTPVITTATDTEGLPSLDLLAAENNMAIARLTAVRHVNAALLEGRQVAVADRHDLLKLKNHPCGLFIHTDEHSLD
NRYAALPSVLVTIHASAARRAPADRLVLHPRILHAGLGCRKNVPAPRAAEHLLRLLEAHDITPLALASLASVEAKRAEPA
MHEASRRLGVPVIFFSSAALAAIAVPNPSSRPLKAVGTPSVAEAAALLAARTNNECRCTQHAAAHLIIPKAADGDVTAAV
AQEQYTMHPAPLYIVGLGPGDPQLLTPQAADALRASEVIAGYTRYVELVPDDILAGREIIATGMMGEMERCAKAIETALS
GRATAVVCSGDPGIYAMAGLIYELIEEMDAYLPVHVIAGVPALSAAAALLGAPLMHDFACISLSDLLTPWEVIEKRLQNA
LEADFVVVLYNPRSKKRDWQLEHALETARRYRQPETPVGLVRQANRPEQAVSVAPLSVFEPAQVDMLSIVIIGNSTTRII
RGNMVTPRGYMKKYR

Sequences:

>Translated_655_residues
MLPERLNGAPAFFTFAHGNIMNSTPRSLHDVSGTDSGGTHTSRLCVFALTPQGIQLAARLCPHLQADLFLPHRLAGLADS
TTAPRTERFLRLADAVRHAFSIYDGLIFIAACGIAVRSIAPLLQGKDRDPAVVVLDQQGLHAVSLLSGHLGGANALACRI
AAMTGGTPVITTATDTEGLPSLDLLAAENNMAIARLTAVRHVNAALLEGRQVAVADRHDLLKLKNHPCGLFIHTDEHSLD
NRYAALPSVLVTIHASAARRAPADRLVLHPRILHAGLGCRKNVPAPRAAEHLLRLLEAHDITPLALASLASVEAKRAEPA
MHEASRRLGVPVIFFSSAALAAIAVPNPSSRPLKAVGTPSVAEAAALLAARTNNECRCTQHAAAHLIIPKAADGDVTAAV
AQEQYTMHPAPLYIVGLGPGDPQLLTPQAADALRASEVIAGYTRYVELVPDDILAGREIIATGMMGEMERCAKAIETALS
GRATAVVCSGDPGIYAMAGLIYELIEEMDAYLPVHVIAGVPALSAAAALLGAPLMHDFACISLSDLLTPWEVIEKRLQNA
LEADFVVVLYNPRSKKRDWQLEHALETARRYRQPETPVGLVRQANRPEQAVSVAPLSVFEPAQVDMLSIVIIGNSTTRII
RGNMVTPRGYMKKYR
>Mature_655_residues
MLPERLNGAPAFFTFAHGNIMNSTPRSLHDVSGTDSGGTHTSRLCVFALTPQGIQLAARLCPHLQADLFLPHRLAGLADS
TTAPRTERFLRLADAVRHAFSIYDGLIFIAACGIAVRSIAPLLQGKDRDPAVVVLDQQGLHAVSLLSGHLGGANALACRI
AAMTGGTPVITTATDTEGLPSLDLLAAENNMAIARLTAVRHVNAALLEGRQVAVADRHDLLKLKNHPCGLFIHTDEHSLD
NRYAALPSVLVTIHASAARRAPADRLVLHPRILHAGLGCRKNVPAPRAAEHLLRLLEAHDITPLALASLASVEAKRAEPA
MHEASRRLGVPVIFFSSAALAAIAVPNPSSRPLKAVGTPSVAEAAALLAARTNNECRCTQHAAAHLIIPKAADGDVTAAV
AQEQYTMHPAPLYIVGLGPGDPQLLTPQAADALRASEVIAGYTRYVELVPDDILAGREIIATGMMGEMERCAKAIETALS
GRATAVVCSGDPGIYAMAGLIYELIEEMDAYLPVHVIAGVPALSAAAALLGAPLMHDFACISLSDLLTPWEVIEKRLQNA
LEADFVVVLYNPRSKKRDWQLEHALETARRYRQPETPVGLVRQANRPEQAVSVAPLSVFEPAQVDMLSIVIIGNSTTRII
RGNMVTPRGYMKKYR

Specific function: Methyltransferase that catalyzes the methylation of C-17 in cobalt-precorrin-3B to form cobalt-precorrin-4 [H]

COG id: COG1010

COG function: function code H; Precorrin-3B methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006363 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: 2.1.1.131

Molecular weight: Translated: 69891; Mature: 69891

Theoretical pI: Translated: 7.53; Mature: 7.53

Prosite motif: PS00699 NITROGENASE_1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPERLNGAPAFFTFAHGNIMNSTPRSLHDVSGTDSGGTHTSRLCVFALTPQGIQLAARL
CCCCCCCCCCEEEEEECCCCCCCCCCHHHCCCCCCCCCCCCCEEEEEEECCCHHHHHHHH
CPHLQADLFLPHRLAGLADSTTAPRTERFLRLADAVRHAFSIYDGLIFIAACGIAVRSIA
CCCCHHHCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PLLQGKDRDPAVVVLDQQGLHAVSLLSGHLGGANALACRIAAMTGGTPVITTATDTEGLP
HHHCCCCCCCEEEEECCCCCHHHHHHHCCCCCCCHHEEEEEEECCCCEEEEECCCCCCCC
SLDLLAAENNMAIARLTAVRHVNAALLEGRQVAVADRHDLLKLKNHPCGLFIHTDEHSLD
CEEEEECCCCCEEEHHHHHHHHHHHHHCCCEEEEECCHHHHHHCCCCCEEEEECCCCCCC
NRYAALPSVLVTIHASAARRAPADRLVLHPRILHAGLGCRKNVPAPRAAEHLLRLLEAHD
CHHHHHHHHHEEEEHHHHHCCCCCCEEECHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCC
ITPLALASLASVEAKRAEPAMHEASRRLGVPVIFFSSAALAAIAVPNPSSRPLKAVGTPS
CCHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEEECCCCEEEEECCCCCCCCEEECCCCC
VAEAAALLAARTNNECRCTQHAAAHLIIPKAADGDVTAAVAQEQYTMHPAPLYIVGLGPG
HHHHHHHHHHCCCCCCEEHHHCCEEEEEECCCCCCEEHHHHHHHCCCCCCCEEEEEECCC
DPQLLTPQAADALRASEVIAGYTRYVELVPDDILAGREIIATGMMGEMERCAKAIETALS
CCCEECCHHHHHHHHHHHHHHHHHHHEECCHHHHCCCHHHHHCCHHHHHHHHHHHHHHHC
GRATAVVCSGDPGIYAMAGLIYELIEEMDAYLPVHVIAGVPALSAAAALLGAPLMHDFAC
CCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHCCHHHHHHHH
ISLSDLLTPWEVIEKRLQNALEADFVVVLYNPRSKKRDWQLEHALETARRYRQPETPVGL
HHHHHHCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCHHH
VRQANRPEQAVSVAPLSVFEPAQVDMLSIVIIGNSTTRIIRGNMVTPRGYMKKYR
HHCCCCCHHHHEECCCCCCCCCCCCEEEEEEECCCCEEEEECCCCCCHHHHHCCC
>Mature Secondary Structure
MLPERLNGAPAFFTFAHGNIMNSTPRSLHDVSGTDSGGTHTSRLCVFALTPQGIQLAARL
CCCCCCCCCCEEEEEECCCCCCCCCCHHHCCCCCCCCCCCCCEEEEEEECCCHHHHHHHH
CPHLQADLFLPHRLAGLADSTTAPRTERFLRLADAVRHAFSIYDGLIFIAACGIAVRSIA
CCCCHHHCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PLLQGKDRDPAVVVLDQQGLHAVSLLSGHLGGANALACRIAAMTGGTPVITTATDTEGLP
HHHCCCCCCCEEEEECCCCCHHHHHHHCCCCCCCHHEEEEEEECCCCEEEEECCCCCCCC
SLDLLAAENNMAIARLTAVRHVNAALLEGRQVAVADRHDLLKLKNHPCGLFIHTDEHSLD
CEEEEECCCCCEEEHHHHHHHHHHHHHCCCEEEEECCHHHHHHCCCCCEEEEECCCCCCC
NRYAALPSVLVTIHASAARRAPADRLVLHPRILHAGLGCRKNVPAPRAAEHLLRLLEAHD
CHHHHHHHHHEEEEHHHHHCCCCCCEEECHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCC
ITPLALASLASVEAKRAEPAMHEASRRLGVPVIFFSSAALAAIAVPNPSSRPLKAVGTPS
CCHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEEECCCCEEEEECCCCCCCCEEECCCCC
VAEAAALLAARTNNECRCTQHAAAHLIIPKAADGDVTAAVAQEQYTMHPAPLYIVGLGPG
HHHHHHHHHHCCCCCCEEHHHCCEEEEEECCCCCCEEHHHHHHHCCCCCCCEEEEEECCC
DPQLLTPQAADALRASEVIAGYTRYVELVPDDILAGREIIATGMMGEMERCAKAIETALS
CCCEECCHHHHHHHHHHHHHHHHHHHEECCHHHHCCCHHHHHCCHHHHHHHHHHHHHHHC
GRATAVVCSGDPGIYAMAGLIYELIEEMDAYLPVHVIAGVPALSAAAALLGAPLMHDFAC
CCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHCCHHHHHHHH
ISLSDLLTPWEVIEKRLQNALEADFVVVLYNPRSKKRDWQLEHALETARRYRQPETPVGL
HHHHHHCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCHHH
VRQANRPEQAVSVAPLSVFEPAQVDMLSIVIIGNSTTRIIRGNMVTPRGYMKKYR
HHCCCCCHHHHEECCCCCCCCCCCCEEEEEEECCCCEEEEECCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 3B

Specific reaction: S-adenosyl-L-methionine + precorrin-3B = S-adenosyl-L-homocysteine + precorrin-4

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8501034; 11677609 [H]