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The map label for this gene is hemL

Identifier: 78358220

GI number: 78358220

Start: 3166349

End: 3167617

Strand: Direct

Name: hemL

Synonym: Dde_3180

Alternate gene names: 78358220

Gene position: 3166349-3167617 (Clockwise)

Preceding gene: 78358219

Following gene: 78358221

Centisome position: 84.88

GC content: 61.15

Gene sequence:

>1269_bases
ATGGAACGTTCCAGAAATCTTTTCGAACGCGCCCAGAAACTTATTCCCGGCGGGGTGAACAGCCCCGTGCGCGCCTGTCT
GAGCGTAGACAGCGCCCCCGTGTTTGTAGAACGCGGGCAGGGCAGCAAGCTGTACAGCGTTGACGGCAGAGAATACATCG
ACTTTGTGGAATCGTGGGGGCCGCTGCTGCTGGGCCATGCCCACCCCGCCGTCAACAAGGCCATCCACGAAGCCGTGGAC
AAGGGGTCGAGTTTCGGCGCCCCCTGCGAAGACGAAATCCGCCTTGCAGAAGCCGTGGTGGACGCCGTGCCCAGCATTGA
GATGGTGCGCATGGTCAGCTCCGGCACAGAAGCCACCATGTCTGCCCTGCGCCTTGCGCGCGGCTACACCGGCCGCAACA
AACTGATTAAATTTGAGGGCTGCTACCACGGCCATGGCGATGCTTTTCTGGCCAGCGCAGGTTCCGGAGTGGCAACGCTT
TCCATTCCGGGAACCCCCGGCGTGCCCGACACAGTGGTGGCGGACACCCTGCTGGCACCCTACAACAACCTTGAACGCGT
AAAAGCCCTGTTTGAAAACCACGGCGCAGACATCGCCGCAGTCATCGTGGAGCCCACGGCGGGCAATATGGGACTGGTAC
GGCCCGCCGCCGGTTTTCTGCAGGGATTGCGCGAACTGTGCGACCGCTACGGCGCGCTGCTTATCTTCGACGAGGTCATC
ACCGGCTTCCGGCTGTCCTACGGCGGGGCACAGCAGTATTTCGGCATCACCCCCGACCTGACCACACTGGGCAAAATCAT
CGGCGGCGGCATGCCCGTGGGCGCCTATGGCGGCAGACGCGAGATAATGGAGCGCATCGCTCCCTGCGGCGATGTGTATC
AGGCCGGCACTCTGGCAGGCAACCCTGTGGCCATGGCCGCAGGTATCGCCACGCTGGCGGAACTGAAAAAAGCCGACTAT
CCCGCACTGGAAAAACGTGTGGACGCGCTTGCTTCTGAAATAGAAGCCATTCTCAAGGCCAAGGGCGTACCCGTGACAGT
CAACCGTCTGGCCTCCATGTTCACCATCTTCTTCACCGAAGAACCGGTGACTGACTATGACAGCGCCAAAAAAGCCGACG
GCACGCTCTACACCCGCTTTTACCAGCAGATGCGTGATCAGGGCATCTACCTTGCCCCGTCCGGTTTTGAATGCGCCATG
GTGTCGCTGGCCCACACAGAAGACGACTTTGCGGCCATGCTCGCAGCAGTGCGCAACGTCACCTTCTAG

Upstream 100 bases:

>100_bases
ACAGCCTGAAAGAACTGAAAAAAACCTCCATGACCTATTTCTAGCGCGGAGGAGCAAATCCGCGGTTGCACAATCGCGGG
CATGACAAGGATACCGCATC

Downstream 100 bases:

>100_bases
GGCAAATCCTGCGGGTATGGTCCCGCCCCCGCAACAATGCTATGCTGCCGGAGCGCCTGAACGGTGCTCCGGCTTTTTTC
ACCTTTGCGCACGGCAACAT

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MERSRNLFERAQKLIPGGVNSPVRACLSVDSAPVFVERGQGSKLYSVDGREYIDFVESWGPLLLGHAHPAVNKAIHEAVD
KGSSFGAPCEDEIRLAEAVVDAVPSIEMVRMVSSGTEATMSALRLARGYTGRNKLIKFEGCYHGHGDAFLASAGSGVATL
SIPGTPGVPDTVVADTLLAPYNNLERVKALFENHGADIAAVIVEPTAGNMGLVRPAAGFLQGLRELCDRYGALLIFDEVI
TGFRLSYGGAQQYFGITPDLTTLGKIIGGGMPVGAYGGRREIMERIAPCGDVYQAGTLAGNPVAMAAGIATLAELKKADY
PALEKRVDALASEIEAILKAKGVPVTVNRLASMFTIFFTEEPVTDYDSAKKADGTLYTRFYQQMRDQGIYLAPSGFECAM
VSLAHTEDDFAAMLAAVRNVTF

Sequences:

>Translated_422_residues
MERSRNLFERAQKLIPGGVNSPVRACLSVDSAPVFVERGQGSKLYSVDGREYIDFVESWGPLLLGHAHPAVNKAIHEAVD
KGSSFGAPCEDEIRLAEAVVDAVPSIEMVRMVSSGTEATMSALRLARGYTGRNKLIKFEGCYHGHGDAFLASAGSGVATL
SIPGTPGVPDTVVADTLLAPYNNLERVKALFENHGADIAAVIVEPTAGNMGLVRPAAGFLQGLRELCDRYGALLIFDEVI
TGFRLSYGGAQQYFGITPDLTTLGKIIGGGMPVGAYGGRREIMERIAPCGDVYQAGTLAGNPVAMAAGIATLAELKKADY
PALEKRVDALASEIEAILKAKGVPVTVNRLASMFTIFFTEEPVTDYDSAKKADGTLYTRFYQQMRDQGIYLAPSGFECAM
VSLAHTEDDFAAMLAAVRNVTF
>Mature_422_residues
MERSRNLFERAQKLIPGGVNSPVRACLSVDSAPVFVERGQGSKLYSVDGREYIDFVESWGPLLLGHAHPAVNKAIHEAVD
KGSSFGAPCEDEIRLAEAVVDAVPSIEMVRMVSSGTEATMSALRLARGYTGRNKLIKFEGCYHGHGDAFLASAGSGVATL
SIPGTPGVPDTVVADTLLAPYNNLERVKALFENHGADIAAVIVEPTAGNMGLVRPAAGFLQGLRELCDRYGALLIFDEVI
TGFRLSYGGAQQYFGITPDLTTLGKIIGGGMPVGAYGGRREIMERIAPCGDVYQAGTLAGNPVAMAAGIATLAELKKADY
PALEKRVDALASEIEAILKAKGVPVTVNRLASMFTIFFTEEPVTDYDSAKKADGTLYTRFYQQMRDQGIYLAPSGFECAM
VSLAHTEDDFAAMLAAVRNVTF

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily

Homologues:

Organism=Homo sapiens, GI4557809, Length=375, Percent_Identity=29.3333333333333, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI13994255, Length=322, Percent_Identity=28.5714285714286, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI37574042, Length=330, Percent_Identity=29.3939393939394, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI24119277, Length=307, Percent_Identity=28.3387622149837, Blast_Score=108, Evalue=8e-24,
Organism=Homo sapiens, GI226442705, Length=331, Percent_Identity=28.7009063444109, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI226442709, Length=297, Percent_Identity=30.3030303030303, Blast_Score=102, Evalue=9e-22,
Organism=Homo sapiens, GI284507298, Length=295, Percent_Identity=28.8135593220339, Blast_Score=87, Evalue=4e-17,
Organism=Escherichia coli, GI1786349, Length=425, Percent_Identity=54.3529411764706, Blast_Score=473, Evalue=1e-134,
Organism=Escherichia coli, GI1789016, Length=416, Percent_Identity=31.25, Blast_Score=158, Evalue=8e-40,
Organism=Escherichia coli, GI1788044, Length=284, Percent_Identity=38.7323943661972, Blast_Score=152, Evalue=5e-38,
Organism=Escherichia coli, GI1789759, Length=281, Percent_Identity=34.8754448398576, Blast_Score=145, Evalue=5e-36,
Organism=Escherichia coli, GI145693181, Length=314, Percent_Identity=31.5286624203822, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1787560, Length=383, Percent_Identity=30.2872062663185, Blast_Score=118, Evalue=6e-28,
Organism=Escherichia coli, GI1786991, Length=393, Percent_Identity=28.7531806615776, Blast_Score=101, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI25144271, Length=360, Percent_Identity=26.6666666666667, Blast_Score=116, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI71992977, Length=311, Percent_Identity=29.5819935691318, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI25144274, Length=182, Percent_Identity=31.8681318681319, Blast_Score=82, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6323470, Length=385, Percent_Identity=25.974025974026, Blast_Score=101, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6324432, Length=325, Percent_Identity=26.7692307692308, Blast_Score=94, Evalue=5e-20,
Organism=Drosophila melanogaster, GI21357415, Length=333, Percent_Identity=28.5285285285285, Blast_Score=99, Evalue=7e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSA_DESDG (Q30WH2)

Other databases:

- EMBL:   CP000112
- RefSeq:   YP_389669.1
- ProteinModelPortal:   Q30WH2
- SMR:   Q30WH2
- STRING:   Q30WH2
- GeneID:   3758155
- GenomeReviews:   CP000112_GR
- KEGG:   dde:Dde_3180
- NMPDR:   fig|207559.3.peg.2675
- eggNOG:   COG0001
- HOGENOM:   HBG725944
- OMA:   HANEKIV
- ProtClustDB:   PRK00062
- BioCyc:   DDES207559:DDE_3180-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00375
- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11986
- TIGRFAMs:   TIGR00713

Pfam domain/function: PF00202 Aminotran_3; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =5.4.3.8

Molecular weight: Translated: 45014; Mature: 45014

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERSRNLFERAQKLIPGGVNSPVRACLSVDSAPVFVERGQGSKLYSVDGREYIDFVESWG
CCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEECCCCCEEEECCCHHHHHHHHCCC
PLLLGHAHPAVNKAIHEAVDKGSSFGAPCEDEIRLAEAVVDAVPSIEMVRMVSSGTEATM
CEEEECCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHH
SALRLARGYTGRNKLIKFEGCYHGHGDAFLASAGSGVATLSIPGTPGVPDTVVADTLLAP
HHHHHHHCCCCCCCEEEEECCCCCCCCEEEEECCCCEEEEECCCCCCCCHHHHHHHHHCC
YNNLERVKALFENHGADIAAVIVEPTAGNMGLVRPAAGFLQGLRELCDRYGALLIFDEVI
CCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCEEHHHHHH
TGFRLSYGGAQQYFGITPDLTTLGKIIGGGMPVGAYGGRREIMERIAPCGDVYQAGTLAG
HHHEECCCCCHHCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCHHHHCCCCCC
NPVAMAAGIATLAELKKADYPALEKRVDALASEIEAILKAKGVPVTVNRLASMFTIFFTE
CCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHEEEC
EPVTDYDSAKKADGTLYTRFYQQMRDQGIYLAPSGFECAMVSLAHTEDDFAAMLAAVRNV
CCCCCCCCCCCCCCHHHHHHHHHHHHCCEEECCCCCCEEEEEHHCCCHHHHHHHHHHHHC
TF
CC
>Mature Secondary Structure
MERSRNLFERAQKLIPGGVNSPVRACLSVDSAPVFVERGQGSKLYSVDGREYIDFVESWG
CCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEECCCCCEEEECCCHHHHHHHHCCC
PLLLGHAHPAVNKAIHEAVDKGSSFGAPCEDEIRLAEAVVDAVPSIEMVRMVSSGTEATM
CEEEECCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHH
SALRLARGYTGRNKLIKFEGCYHGHGDAFLASAGSGVATLSIPGTPGVPDTVVADTLLAP
HHHHHHHCCCCCCCEEEEECCCCCCCCEEEEECCCCEEEEECCCCCCCCHHHHHHHHHCC
YNNLERVKALFENHGADIAAVIVEPTAGNMGLVRPAAGFLQGLRELCDRYGALLIFDEVI
CCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCEEHHHHHH
TGFRLSYGGAQQYFGITPDLTTLGKIIGGGMPVGAYGGRREIMERIAPCGDVYQAGTLAG
HHHEECCCCCHHCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCHHHHCCCCCC
NPVAMAAGIATLAELKKADYPALEKRVDALASEIEAILKAKGVPVTVNRLASMFTIFFTE
CCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHEEEC
EPVTDYDSAKKADGTLYTRFYQQMRDQGIYLAPSGFECAMVSLAHTEDDFAAMLAAVRNV
CCCCCCCCCCCCCCHHHHHHHHHHHHCCEEECCCCCCEEEEEHHCCCHHHHHHHHHHHHC
TF
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA