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The map label for this gene is truB

Identifier: 78358205

GI number: 78358205

Start: 3152976

End: 3153905

Strand: Direct

Name: truB

Synonym: Dde_3165

Alternate gene names: 78358205

Gene position: 3152976-3153905 (Clockwise)

Preceding gene: 78358204

Following gene: 78358206

Centisome position: 84.52

GC content: 58.92

Gene sequence:

>930_bases
ATGGCTGATTCGAAACTTGCACAGCAGCACGGGGTACTGGTACTTAACAAACCTAAAGGCCCCACATCGGCTCACTGCAT
CGCCAGAATCAAACGGCTGGGGCAGAAAAAAATAGGCCACGCCGGCACGCTGGACCCCATGGCTCAGGGCGTGCTGCTGG
TGCTGCTGGGCCAGTGCACCAAGATTTCCGGCTATCTGATGGAAGGCGGAGAAAAAATATACTCCGGCACGCTGGAACTG
GGACGCACCACAGACACGTGGGACGACGAAGGCGAAACACTTTCCACAGCAGACTGGACGCACGTAACCGAAGAGGACGT
GGTCCGCGCTGTCGACTTGTGGACAGGTTCGTCGGAACAGCAGGTTCCGGCATATTCCGCGGCCAAGCACAAGGGGCAGC
CCCTGTACAAGCTGGCACGCGAAGGTAAGGAAACGCCTGTCAAAACAAGGCGTATAGAAATTTCACAGGCGGAAACGCTC
GCTGTTGAGCTGCCGTTTGTGCGCTTCCGGGTTCATTGCAGTTCGGGCACCTATATACGATCCCTGGCCCACAGCTTGGG
GAACCGTCTAGGGTGCGGCGCTGTGCTGACCGAACTCATCCGGGAGTACAGCCACCCGTTCTCGCTGGACGAAGCCCATG
ATCTGGACGACGTTCTGGCAGAACCGGCGGAACTTGCCGGCAGGGTCATACCGCTGGACAAGGCATTGCCGCACTGGCCC
AAGCTGCGCCTGAGCGCAGCTGACGAAGCCCGTGTGAAAAACGGCATGCCCCATCCGTATGATCCGGCAGAAATGGCCAG
CATGCCTTTTACCGAAGGTATCAGAGCCGTACTGCTCGACCCGGCCGGTGACCCGCTGGCTCTGGCGGAAACAGCCTACA
GAAATCAGGTGCCCGTCTGGACGGTTCTGCGGGGGCTGTGGAATACATAA

Upstream 100 bases:

>100_bases
GCCGGTGCCGAACTGGACATGCCGCTGGAATCCGCCGTGCAGGAAATACTTTCATCCGTCTCGCGCAATCTTGCACTGGA
CGCCGAGGGCGCTTAACTGT

Downstream 100 bases:

>100_bases
TATGTTACTCGGAGGTAACTTGCTGTGGTAATGACTCCTGAAAAGAAGATGTCCATCATTGAAGAATACGGTCAGCACGA
AGGCGATACCGGTTCTCCCG

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 309; Mature: 308

Protein sequence:

>309_residues
MADSKLAQQHGVLVLNKPKGPTSAHCIARIKRLGQKKIGHAGTLDPMAQGVLLVLLGQCTKISGYLMEGGEKIYSGTLEL
GRTTDTWDDEGETLSTADWTHVTEEDVVRAVDLWTGSSEQQVPAYSAAKHKGQPLYKLAREGKETPVKTRRIEISQAETL
AVELPFVRFRVHCSSGTYIRSLAHSLGNRLGCGAVLTELIREYSHPFSLDEAHDLDDVLAEPAELAGRVIPLDKALPHWP
KLRLSAADEARVKNGMPHPYDPAEMASMPFTEGIRAVLLDPAGDPLALAETAYRNQVPVWTVLRGLWNT

Sequences:

>Translated_309_residues
MADSKLAQQHGVLVLNKPKGPTSAHCIARIKRLGQKKIGHAGTLDPMAQGVLLVLLGQCTKISGYLMEGGEKIYSGTLEL
GRTTDTWDDEGETLSTADWTHVTEEDVVRAVDLWTGSSEQQVPAYSAAKHKGQPLYKLAREGKETPVKTRRIEISQAETL
AVELPFVRFRVHCSSGTYIRSLAHSLGNRLGCGAVLTELIREYSHPFSLDEAHDLDDVLAEPAELAGRVIPLDKALPHWP
KLRLSAADEARVKNGMPHPYDPAEMASMPFTEGIRAVLLDPAGDPLALAETAYRNQVPVWTVLRGLWNT
>Mature_308_residues
ADSKLAQQHGVLVLNKPKGPTSAHCIARIKRLGQKKIGHAGTLDPMAQGVLLVLLGQCTKISGYLMEGGEKIYSGTLELG
RTTDTWDDEGETLSTADWTHVTEEDVVRAVDLWTGSSEQQVPAYSAAKHKGQPLYKLAREGKETPVKTRRIEISQAETLA
VELPFVRFRVHCSSGTYIRSLAHSLGNRLGCGAVLTELIREYSHPFSLDEAHDLDDVLAEPAELAGRVIPLDKALPHWPK
LRLSAADEARVKNGMPHPYDPAEMASMPFTEGIRAVLLDPAGDPLALAETAYRNQVPVWTVLRGLWNT

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=258, Percent_Identity=34.4961240310078, Blast_Score=123, Evalue=2e-28,
Organism=Escherichia coli, GI2367200, Length=260, Percent_Identity=33.8461538461538, Blast_Score=139, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI17553978, Length=195, Percent_Identity=29.7435897435897, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324037, Length=128, Percent_Identity=35.15625, Blast_Score=83, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6323204, Length=220, Percent_Identity=30.4545454545455, Blast_Score=70, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_DESDG (Q30WI7)

Other databases:

- EMBL:   CP000112
- RefSeq:   YP_389654.1
- ProteinModelPortal:   Q30WI7
- SMR:   Q30WI7
- STRING:   Q30WI7
- GeneID:   3758140
- GenomeReviews:   CP000112_GR
- KEGG:   dde:Dde_3165
- NMPDR:   fig|207559.3.peg.2661
- eggNOG:   COG0130
- HOGENOM:   HBG397258
- OMA:   YPAHMSK
- ProtClustDB:   CLSK2522478
- BioCyc:   DDES207559:DDE_3165-MONOMER
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom

EC number: 4.2.1.70

Molecular weight: Translated: 33841; Mature: 33710

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: NA

Important sites: ACT_SITE 45-45

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADSKLAQQHGVLVLNKPKGPTSAHCIARIKRLGQKKIGHAGTLDPMAQGVLLVLLGQCT
CCCHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
KISGYLMEGGEKIYSGTLELGRTTDTWDDEGETLSTADWTHVTEEDVVRAVDLWTGSSEQ
HHHHHHHHCCCEEECCHHCCCCCCCCCCCCCCEECCCCCCCCCHHHHHHHHHHCCCCCCC
QVPAYSAAKHKGQPLYKLAREGKETPVKTRRIEISQAETLAVELPFVRFRVHCSSGTYIR
CCCCHHHHHHCCCHHHHHHHCCCCCCCCEEEEEECCCCEEEEEECEEEEEEEECCCHHHH
SLAHSLGNRLGCGAVLTELIREYSHPFSLDEAHDLDDVLAEPAELAGRVIPLDKALPHWP
HHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCHHHHCCCEEECHHCCCCCC
KLRLSAADEARVKNGMPHPYDPAEMASMPFTEGIRAVLLDPAGDPLALAETAYRNQVPVW
CEEECCCHHHHHHCCCCCCCCHHHHHCCCHHHCCEEEEECCCCCCHHHHHHHHCCCCCHH
TVLRGLWNT
HHHHHHCCC
>Mature Secondary Structure 
ADSKLAQQHGVLVLNKPKGPTSAHCIARIKRLGQKKIGHAGTLDPMAQGVLLVLLGQCT
CCHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
KISGYLMEGGEKIYSGTLELGRTTDTWDDEGETLSTADWTHVTEEDVVRAVDLWTGSSEQ
HHHHHHHHCCCEEECCHHCCCCCCCCCCCCCCEECCCCCCCCCHHHHHHHHHHCCCCCCC
QVPAYSAAKHKGQPLYKLAREGKETPVKTRRIEISQAETLAVELPFVRFRVHCSSGTYIR
CCCCHHHHHHCCCHHHHHHHCCCCCCCCEEEEEECCCCEEEEEECEEEEEEEECCCHHHH
SLAHSLGNRLGCGAVLTELIREYSHPFSLDEAHDLDDVLAEPAELAGRVIPLDKALPHWP
HHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCHHHHCCCEEECHHCCCCCC
KLRLSAADEARVKNGMPHPYDPAEMASMPFTEGIRAVLLDPAGDPLALAETAYRNQVPVW
CEEECCCHHHHHHCCCCCCCCHHHHHCCCHHHCCEEEEECCCCCCHHHHHHHHCCCCCHH
TVLRGLWNT
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA