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The map label for this gene is nusA

Identifier: 78358201

GI number: 78358201

Start: 3147059

End: 3148348

Strand: Direct

Name: nusA

Synonym: Dde_3161

Alternate gene names: 78358201

Gene position: 3147059-3148348 (Clockwise)

Preceding gene: 78358200

Following gene: 78358202

Centisome position: 84.37

GC content: 58.06

Gene sequence:

>1290_bases
ATGAGCATGGAATTGAAAAAAGCCATAGACCAGATCAGCAAAGACCGGGGGCTGGACCGGGCACTGCTGATCGAAACGCT
GGAAGAAGCGGTACGTACCTCCGTCATCCGCAAATACGGCGATGAGCTGGACCTTGAAGTCAACTTCAATGAAGAAACAG
GCGAAATCGAGGTTTACCAGTTCAAAATCGTGACGGAAGAAGTGGAGGACGAGGCTTCGCAGATTTCTCTGGAAGACGCC
CGCGCACACGACCCCAGCGTCCAGCTTGACGACGAGATGGGCTTTCGCCTGAAAATCGAAGATCTGGGCCGTATTGCCGC
CCAGTCCGCCAAGCAGGTCATCATCCAGCGCATGCGGGATGCCGAGCAGGAAATCATATACGAAGAATTCAAGGACCGCC
TGAACGAAGTCGCCAGCGGCATCATTCAGCGCAGAGACAAAACCGGCTGGATCATCAATCTGGGCCGTACCGAAGCGCTG
CTGCCCAAAGACGAACAGATTCCGCGCGAACACTACAAGCGCGGCGACCGCGTGCAGGCCATCATCATAGAAGTGCGCAA
GGAAGGCCGCGGCCCGCAGGTTGTGGTATCCCGTTCGCACCCCGACTACATGGCCGCGCTGTTCAAACGCGAAGTGCCCG
AAGTGGATGACGAAACCGTTCAGGTGATGGGTGTTGCCCGCGATCCGGGCAGCCGCGCCAAAGTGGCTGTCACCTCGCGG
GACCGCGATGTCGATCCGGTGGGTGCCTGTGTGGGCATACGCGGTTCGCGCATTCAGAATATCGTGCAGGAACTGCGCGG
CGAACGCATCGACATAGTGGTGTGGAGTCCCGACATCGCAACCTATGCACGCAACGCCCTCAGCCCCGCCGTGATCAGCC
GCATCATTGTGGACGAAGAGGAAAACATGCTCGAAGTGGTTGTGCCTGACGATCAGCTGACCAATGCCATCGGCAGAAAG
GGTCAGAACGTCAAACTGGCATCCAAACTGCTGGGCTGGAAAATAGATATTTACACCGAGACCCGCTACAACGAAGCCAA
CGCCATAGGGCGCGGTCTCGAACAGCTGGCCAGCGTGGCCGAAGTGCCCATCGAGCAGTTTGTGGCCGCGGGCTTCTCGT
CCATAGAAGAGCTGCGCGACGCCACAGACGAAGAGCTGATGGCCGTGGAAGGCCTTACTCCCGGAAAGATCTCGGATCTG
CGGGCTGCCATCAACTTCCTTGCCCCTGTACAGCGCAACGCTGAAGACGGAGAGGAAGAAGAAGCCGCTGACGGGGAAAA
CGCCGAATAG

Upstream 100 bases:

>100_bases
GGACGATGTGAAAAAAGCCAGACTGATCTACGATTTTGACAGCGAAAAAGGCCAGAAGCGCTAACGGCCGCCCGGACATA
TCGAGTCGGAGGAATTCCCC

Downstream 100 bases:

>100_bases
TCCGGAAGCCGATGACTGACGGACAAAAACATATTCCGATACGCACCTGCGTCATCTGCAGGAGACGGTTTGCAAAGAGC
GATCTCCTGCGCTACGTCTG

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MSMELKKAIDQISKDRGLDRALLIETLEEAVRTSVIRKYGDELDLEVNFNEETGEIEVYQFKIVTEEVEDEASQISLEDA
RAHDPSVQLDDEMGFRLKIEDLGRIAAQSAKQVIIQRMRDAEQEIIYEEFKDRLNEVASGIIQRRDKTGWIINLGRTEAL
LPKDEQIPREHYKRGDRVQAIIIEVRKEGRGPQVVVSRSHPDYMAALFKREVPEVDDETVQVMGVARDPGSRAKVAVTSR
DRDVDPVGACVGIRGSRIQNIVQELRGERIDIVVWSPDIATYARNALSPAVISRIIVDEEENMLEVVVPDDQLTNAIGRK
GQNVKLASKLLGWKIDIYTETRYNEANAIGRGLEQLASVAEVPIEQFVAAGFSSIEELRDATDEELMAVEGLTPGKISDL
RAAINFLAPVQRNAEDGEEEEAADGENAE

Sequences:

>Translated_429_residues
MSMELKKAIDQISKDRGLDRALLIETLEEAVRTSVIRKYGDELDLEVNFNEETGEIEVYQFKIVTEEVEDEASQISLEDA
RAHDPSVQLDDEMGFRLKIEDLGRIAAQSAKQVIIQRMRDAEQEIIYEEFKDRLNEVASGIIQRRDKTGWIINLGRTEAL
LPKDEQIPREHYKRGDRVQAIIIEVRKEGRGPQVVVSRSHPDYMAALFKREVPEVDDETVQVMGVARDPGSRAKVAVTSR
DRDVDPVGACVGIRGSRIQNIVQELRGERIDIVVWSPDIATYARNALSPAVISRIIVDEEENMLEVVVPDDQLTNAIGRK
GQNVKLASKLLGWKIDIYTETRYNEANAIGRGLEQLASVAEVPIEQFVAAGFSSIEELRDATDEELMAVEGLTPGKISDL
RAAINFLAPVQRNAEDGEEEEAADGENAE
>Mature_428_residues
SMELKKAIDQISKDRGLDRALLIETLEEAVRTSVIRKYGDELDLEVNFNEETGEIEVYQFKIVTEEVEDEASQISLEDAR
AHDPSVQLDDEMGFRLKIEDLGRIAAQSAKQVIIQRMRDAEQEIIYEEFKDRLNEVASGIIQRRDKTGWIINLGRTEALL
PKDEQIPREHYKRGDRVQAIIIEVRKEGRGPQVVVSRSHPDYMAALFKREVPEVDDETVQVMGVARDPGSRAKVAVTSRD
RDVDPVGACVGIRGSRIQNIVQELRGERIDIVVWSPDIATYARNALSPAVISRIIVDEEENMLEVVVPDDQLTNAIGRKG
QNVKLASKLLGWKIDIYTETRYNEANAIGRGLEQLASVAEVPIEQFVAAGFSSIEELRDATDEELMAVEGLTPGKISDLR
AAINFLAPVQRNAEDGEEEEAADGENAE

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=438, Percent_Identity=36.3013698630137, Blast_Score=292, Evalue=4e-80,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 48048; Mature: 47916

Theoretical pI: Translated: 4.33; Mature: 4.33

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMELKKAIDQISKDRGLDRALLIETLEEAVRTSVIRKYGDELDLEVNFNEETGEIEVYQ
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEEEE
FKIVTEEVEDEASQISLEDARAHDPSVQLDDEMGFRLKIEDLGRIAAQSAKQVIIQRMRD
EEEEHHHHHHHHHHEEHHHHCCCCCCCEECCCCCCEEEHHHHHHHHHHHHHHHHHHHHHH
AEQEIIYEEFKDRLNEVASGIIQRRDKTGWIINLGRTEALLPKDEQIPREHYKRGDRVQA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCHHHHCCCCHHHH
IIIEVRKEGRGPQVVVSRSHPDYMAALFKREVPEVDDETVQVMGVARDPGSRAKVAVTSR
HHEEHHHCCCCCEEEEECCCCHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCEEEEECC
DRDVDPVGACVGIRGSRIQNIVQELRGERIDIVVWSPDIATYARNALSPAVISRIIVDEE
CCCCCHHHHHHCCCHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHCCCHHHHHHHHHCCC
ENMLEVVVPDDQLTNAIGRKGQNVKLASKLLGWKIDIYTETRYNEANAIGRGLEQLASVA
CCEEEEEECCHHHHHHHCCCCCCHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHHHH
EVPIEQFVAAGFSSIEELRDATDEELMAVEGLTPGKISDLRAAINFLAPVQRNAEDGEEE
HCCHHHHHHHHHHHHHHHHCCCCHHHHEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCHH
EAADGENAE
HCCCCCCCC
>Mature Secondary Structure 
SMELKKAIDQISKDRGLDRALLIETLEEAVRTSVIRKYGDELDLEVNFNEETGEIEVYQ
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEEEE
FKIVTEEVEDEASQISLEDARAHDPSVQLDDEMGFRLKIEDLGRIAAQSAKQVIIQRMRD
EEEEHHHHHHHHHHEEHHHHCCCCCCCEECCCCCCEEEHHHHHHHHHHHHHHHHHHHHHH
AEQEIIYEEFKDRLNEVASGIIQRRDKTGWIINLGRTEALLPKDEQIPREHYKRGDRVQA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCHHHHCCCCHHHH
IIIEVRKEGRGPQVVVSRSHPDYMAALFKREVPEVDDETVQVMGVARDPGSRAKVAVTSR
HHEEHHHCCCCCEEEEECCCCHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCEEEEECC
DRDVDPVGACVGIRGSRIQNIVQELRGERIDIVVWSPDIATYARNALSPAVISRIIVDEE
CCCCCHHHHHHCCCHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHCCCHHHHHHHHHCCC
ENMLEVVVPDDQLTNAIGRKGQNVKLASKLLGWKIDIYTETRYNEANAIGRGLEQLASVA
CCEEEEEECCHHHHHHHCCCCCCHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHHHH
EVPIEQFVAAGFSSIEELRDATDEELMAVEGLTPGKISDLRAAINFLAPVQRNAEDGEEE
HCCHHHHHHHHHHHHHHHHCCCCHHHHEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCHH
EAADGENAE
HCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]