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The map label for this gene is dus1 [H]

Identifier: 78358129

GI number: 78358129

Start: 3079367

End: 3080497

Strand: Reverse

Name: dus1 [H]

Synonym: Dde_3089

Alternate gene names: 78358129

Gene position: 3080497-3079367 (Counterclockwise)

Preceding gene: 78358130

Following gene: 78358128

Centisome position: 82.58

GC content: 61.45

Gene sequence:

>1131_bases
ATGATGCAGCCGGAAGAATATACAGCAGCCGATGCCGTTGAATCCTGTCCGCAGCAGAGTCTTTCCGGACACGGGCCGGA
TCTGGCCGTGGCCGCTGACGGGGTTTTTGACGCCGCCCTTGCCGCCCGTCTGACTGAACCGCTGCAGATAGGCGGCAGAA
CGGTGAACGGCCGTCTGTTTCTGGCTCCCATGGCCGGTCTCGGGCATATTGCCTACCGGCAGGTGCTCGAAAGCTTCGGG
GGATGCGGGCTGGTGTTTACGGGCATGTGCAGCGCACGCGCGGTGCCTACGGAAAATCCGGCGCGGTCTCCGGTGTTCAG
CTGGCGTCCGGAAGAACTGCCCGCGCTGGTCTGCCAGCTTTTCGGTGCCGATCCGCAGGATATGGCTGCGGCTGCCCGCA
GGGTTGAGTCCGAAGGCTTTTTCGGGGTGGACATCAACATGGGCTGTTCTGTGGCGCCTATTGTGAAAAAAGGCTGCGGA
GCGGATCTGCTGCGTGATCCGGAGCGCGCTGTGCGCATGGTGGATGCCGTGCGCCGCGCTGTGTCTGTGCCTGTCTTTGT
CAAAATGCGCACAGGGTGGAGCCCTGATCCGGAACCGGCGGTGAATCTGGCCCGCAGGCTTCAGGACGCCGGTGCCGATG
CGCTGGTCTTTCACCCCCGTGTGGCGCCCGACAGGCGCACCCATCGCCCCAGAACGGCGCATATCGGTCTGGTGAAAGAC
GCGGTGGATATTCCTGTTATCGGCAACGGTGATGTTTTTGATGCAGCGGATTGCGCCGCCATGCTGGAACGCACAGGCTG
CGACGGTGTTTCGCTGGGCAGACTGGCCATAGCCCGCCCGTGGATTTTTGCACAGTGGACGCGCGGTTTTGTTCCGGAAA
AGCAGACATACCGCGACACGCTTTTTGCGCTGATGGATGCGCTGGAAGCGCATTATGATCCTGCCCGCGCCATCAAACTG
TATAAAAAGCTCATCATGTACTACGCGGCCAATTTCACGTTCGGAAACAGGCTGTTCGGCAGGCTTATCCGCGGTGACGA
CATGCAGGCCATGCGGCGCAATGCACAGGCGGAGTTCTGCCGGGTGCCGCAGGTCGGTGCACGTCCCAACCTGCTGATGT
TCTGCATGTGA

Upstream 100 bases:

>100_bases
GGTGCTGGAAGACGGAGTGTTCCGCGTATGCGGTCTGGACGGTTCCGTACTGCTTACCGATACGCTTGGCTAGCGGCGCC
GCGGGCGCAGAGGAAATCTT

Downstream 100 bases:

>100_bases
CGCTGCGCACGGCACCGGAGTGCCGTCTGATGTTCTGTTTTGCAAAAGACCCGCCCGCGGCGGGTCTTTTCTGTTTGCGC
GCAGTCTGTACTTATAACGT

Product: dihydrouridine synthase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MMQPEEYTAADAVESCPQQSLSGHGPDLAVAADGVFDAALAARLTEPLQIGGRTVNGRLFLAPMAGLGHIAYRQVLESFG
GCGLVFTGMCSARAVPTENPARSPVFSWRPEELPALVCQLFGADPQDMAAAARRVESEGFFGVDINMGCSVAPIVKKGCG
ADLLRDPERAVRMVDAVRRAVSVPVFVKMRTGWSPDPEPAVNLARRLQDAGADALVFHPRVAPDRRTHRPRTAHIGLVKD
AVDIPVIGNGDVFDAADCAAMLERTGCDGVSLGRLAIARPWIFAQWTRGFVPEKQTYRDTLFALMDALEAHYDPARAIKL
YKKLIMYYAANFTFGNRLFGRLIRGDDMQAMRRNAQAEFCRVPQVGARPNLLMFCM

Sequences:

>Translated_376_residues
MMQPEEYTAADAVESCPQQSLSGHGPDLAVAADGVFDAALAARLTEPLQIGGRTVNGRLFLAPMAGLGHIAYRQVLESFG
GCGLVFTGMCSARAVPTENPARSPVFSWRPEELPALVCQLFGADPQDMAAAARRVESEGFFGVDINMGCSVAPIVKKGCG
ADLLRDPERAVRMVDAVRRAVSVPVFVKMRTGWSPDPEPAVNLARRLQDAGADALVFHPRVAPDRRTHRPRTAHIGLVKD
AVDIPVIGNGDVFDAADCAAMLERTGCDGVSLGRLAIARPWIFAQWTRGFVPEKQTYRDTLFALMDALEAHYDPARAIKL
YKKLIMYYAANFTFGNRLFGRLIRGDDMQAMRRNAQAEFCRVPQVGARPNLLMFCM
>Mature_376_residues
MMQPEEYTAADAVESCPQQSLSGHGPDLAVAADGVFDAALAARLTEPLQIGGRTVNGRLFLAPMAGLGHIAYRQVLESFG
GCGLVFTGMCSARAVPTENPARSPVFSWRPEELPALVCQLFGADPQDMAAAARRVESEGFFGVDINMGCSVAPIVKKGCG
ADLLRDPERAVRMVDAVRRAVSVPVFVKMRTGWSPDPEPAVNLARRLQDAGADALVFHPRVAPDRRTHRPRTAHIGLVKD
AVDIPVIGNGDVFDAADCAAMLERTGCDGVSLGRLAIARPWIFAQWTRGFVPEKQTYRDTLFALMDALEAHYDPARAIKL
YKKLIMYYAANFTFGNRLFGRLIRGDDMQAMRRNAQAEFCRVPQVGARPNLLMFCM

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=244, Percent_Identity=30.327868852459, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI31742496, Length=225, Percent_Identity=30.2222222222222, Blast_Score=93, Evalue=4e-19,
Organism=Homo sapiens, GI239788462, Length=244, Percent_Identity=30.327868852459, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI8923374, Length=245, Percent_Identity=28.5714285714286, Blast_Score=83, Evalue=4e-16,
Organism=Homo sapiens, GI40807366, Length=242, Percent_Identity=28.5123966942149, Blast_Score=70, Evalue=4e-12,
Organism=Escherichia coli, GI1789660, Length=296, Percent_Identity=32.4324324324324, Blast_Score=134, Evalue=9e-33,
Organism=Escherichia coli, GI1788462, Length=238, Percent_Identity=32.3529411764706, Blast_Score=102, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI25144369, Length=222, Percent_Identity=29.7297297297297, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17543114, Length=287, Percent_Identity=25.0871080139373, Blast_Score=84, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI17507177, Length=234, Percent_Identity=28.6324786324786, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17510279, Length=221, Percent_Identity=27.1493212669683, Blast_Score=69, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6323437, Length=260, Percent_Identity=26.1538461538462, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6323560, Length=236, Percent_Identity=27.9661016949153, Blast_Score=73, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6323433, Length=294, Percent_Identity=23.8095238095238, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI19921524, Length=228, Percent_Identity=31.140350877193, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24585320, Length=251, Percent_Identity=29.4820717131474, Blast_Score=85, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24580595, Length=232, Percent_Identity=29.7413793103448, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI19920448, Length=232, Percent_Identity=29.7413793103448, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI45549423, Length=245, Percent_Identity=28.5714285714286, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 41011; Mature: 41011

Theoretical pI: Translated: 7.90; Mature: 7.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMQPEEYTAADAVESCPQQSLSGHGPDLAVAADGVFDAALAARLTEPLQIGGRTVNGRLF
CCCCCCCHHHHHHHHCHHHHHCCCCCCEEEECCCHHHHHHHHHHCCHHHHCCEEECCEEE
LAPMAGLGHIAYRQVLESFGGCGLVFTGMCSARAVPTENPARSPVFSWRPEELPALVCQL
EECCCHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
FGADPQDMAAAARRVESEGFFGVDINMGCSVAPIVKKGCGADLLRDPERAVRMVDAVRRA
HCCCHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHCCCCCHHHCCHHHHHHHHHHHHHH
VSVPVFVKMRTGWSPDPEPAVNLARRLQDAGADALVFHPRVAPDRRTHRPRTAHIGLVKD
HCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCEEEEEEHH
AVDIPVIGNGDVFDAADCAAMLERTGCDGVSLGRLAIARPWIFAQWTRGFVPEKQTYRDT
CCCCCEECCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHCCHHHHHHHCCCCCCHHHHHHH
LFALMDALEAHYDPARAIKLYKKLIMYYAANFTFGNRLFGRLIRGDDMQAMRRNAQAEFC
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHCCHHHHH
RVPQVGARPNLLMFCM
CCCCCCCCCCEEEEEC
>Mature Secondary Structure
MMQPEEYTAADAVESCPQQSLSGHGPDLAVAADGVFDAALAARLTEPLQIGGRTVNGRLF
CCCCCCCHHHHHHHHCHHHHHCCCCCCEEEECCCHHHHHHHHHHCCHHHHCCEEECCEEE
LAPMAGLGHIAYRQVLESFGGCGLVFTGMCSARAVPTENPARSPVFSWRPEELPALVCQL
EECCCHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
FGADPQDMAAAARRVESEGFFGVDINMGCSVAPIVKKGCGADLLRDPERAVRMVDAVRRA
HCCCHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHCCCCCHHHCCHHHHHHHHHHHHHH
VSVPVFVKMRTGWSPDPEPAVNLARRLQDAGADALVFHPRVAPDRRTHRPRTAHIGLVKD
HCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCEEEEEEHH
AVDIPVIGNGDVFDAADCAAMLERTGCDGVSLGRLAIARPWIFAQWTRGFVPEKQTYRDT
CCCCCEECCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHCCHHHHHHHCCCCCCHHHHHHH
LFALMDALEAHYDPARAIKLYKKLIMYYAANFTFGNRLFGRLIRGDDMQAMRRNAQAEFC
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHCCHHHHH
RVPQVGARPNLLMFCM
CCCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]