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The map label for this gene is folC [H]

Identifier: 78358098

GI number: 78358098

Start: 3048626

End: 3049933

Strand: Direct

Name: folC [H]

Synonym: Dde_3058

Alternate gene names: 78358098

Gene position: 3048626-3049933 (Clockwise)

Preceding gene: 78358097

Following gene: 78358099

Centisome position: 81.73

GC content: 60.7

Gene sequence:

>1308_bases
ATGAACGACAACGCTGCGGATTTTGATACCACCATGCCTCAGGGGCCTTTTACCAGCTACGCCGACGTGCTGGCGCATCT
GGACAATCTGGGGCTGTTTCACATGGAGCTTGATCTGGCGCGCATCAAACGCGTGCTGGAAGAGCTGGAGCTTACCCGTC
CGGAATACCCTGTGGTGCAGGTTGTGGGCACCAACGGTAAAGGTTCCACAGCCACCTTCATGGCTTCCATTGCCACCGGA
GCCGGATTCAGCACGGGCCTGTTCATCTCTCCTCATTTTATCACCCCGCGCGAACGCGTGCTGGTGGACGGCGAGATGCT
GCCCGAAGAAGACTGGCTGGTACTGGCCAACGATATTATGGCCGCCGGCGGTGAAGTGCTGACGTATTTTGAATTTCTGA
CCGTGCTGGCTGCACTGGCCTTTGCAGACTACGAAGTTGATCTGGCTGTTTTTGAAGCCGGTCTGGGGGGCACGTACGAT
GCCACCACAGCCATAGCGGCCGATACCGTGGTATTCACCCCCGTGGCGCTGGATCATCAGCAGATTCTCGGTGACACCGT
TGAAAAAATAGCCGCGGACAAAGCCGGTGCCATCCGCAGCAATGTGCCTGTCGTGTCGGCCCGCCAGACTGATGCCGTGC
TTGAAATACTGAAAAATGCCAGCAATACCGCCGGAGCCCCATTTACAGTGGCTGCCGGCCCCGATTCACTGCCGGAAGAG
GCCCGCCCGGTGCTGAACGGTGTCCACCAGCTGCACAACGCGGCGCTGGCCGTGGCGGCCCTGCAGACCGTCATGGATGA
AAAAGCCCTTCCGCGTCCCACTCTCATCCCCTGGTCCGATGTGGTAACGCTGGGCATAGCCGATGCGTGGATAGCGGGCC
GCATGCAGCACATCGCACCTGCCCCGGACCACCCGGCGCTCATTCTGGACGGCGGCCACAATCTGCACGGGCTCCGTGCG
CTGCAGGCAGCCCTCGCGGCGGAAAATATCCGCCCTGCTGCAATCATTTTCGGCGCCATGAAAGATAAAAACCTTGAAGG
AATACCGGACCTGCTGTCGGCCCTGACCGACGGGCCGGTGCTGCTGCCCGCCCTGACGCAGAACTCACGCGCCGCAACCC
CGCAGGAGCTGGCCGTGCGGCTGGGCGGCGCCCCGGACGGGCCGACAGAAACAATACCCTGCGCATCCGTGCGTGAAGCG
CTGGACAAGGCCGCCGCATACGCTGCGGAAAATCCTGTGCTTATCTGCGGCTCCTTGTATTTGCTGGCCGACTTCTTTAC
ACTCCATCCGGAATATCTGAGACCGTAA

Upstream 100 bases:

>100_bases
AGCGTGTTTATGACGTATTTATATTGACGGACGCGGCCGCATCCCATACGGGTGCTTTGCACAGGGCATGCGCCCGCATC
ACGCCACGGAGTGACAAGAC

Downstream 100 bases:

>100_bases
GACGGATGAGGTTCCGGCGGGTTAGCCGGTCTGCGGCCGGCAAGCCCCTTGCTGTGCATACGCGGCGACCGGCTGAACCC
GGACCATGCTGCGCCGTACT

Product: FolC bifunctional protein

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MNDNAADFDTTMPQGPFTSYADVLAHLDNLGLFHMELDLARIKRVLEELELTRPEYPVVQVVGTNGKGSTATFMASIATG
AGFSTGLFISPHFITPRERVLVDGEMLPEEDWLVLANDIMAAGGEVLTYFEFLTVLAALAFADYEVDLAVFEAGLGGTYD
ATTAIAADTVVFTPVALDHQQILGDTVEKIAADKAGAIRSNVPVVSARQTDAVLEILKNASNTAGAPFTVAAGPDSLPEE
ARPVLNGVHQLHNAALAVAALQTVMDEKALPRPTLIPWSDVVTLGIADAWIAGRMQHIAPAPDHPALILDGGHNLHGLRA
LQAALAAENIRPAAIIFGAMKDKNLEGIPDLLSALTDGPVLLPALTQNSRAATPQELAVRLGGAPDGPTETIPCASVREA
LDKAAAYAAENPVLICGSLYLLADFFTLHPEYLRP

Sequences:

>Translated_435_residues
MNDNAADFDTTMPQGPFTSYADVLAHLDNLGLFHMELDLARIKRVLEELELTRPEYPVVQVVGTNGKGSTATFMASIATG
AGFSTGLFISPHFITPRERVLVDGEMLPEEDWLVLANDIMAAGGEVLTYFEFLTVLAALAFADYEVDLAVFEAGLGGTYD
ATTAIAADTVVFTPVALDHQQILGDTVEKIAADKAGAIRSNVPVVSARQTDAVLEILKNASNTAGAPFTVAAGPDSLPEE
ARPVLNGVHQLHNAALAVAALQTVMDEKALPRPTLIPWSDVVTLGIADAWIAGRMQHIAPAPDHPALILDGGHNLHGLRA
LQAALAAENIRPAAIIFGAMKDKNLEGIPDLLSALTDGPVLLPALTQNSRAATPQELAVRLGGAPDGPTETIPCASVREA
LDKAAAYAAENPVLICGSLYLLADFFTLHPEYLRP
>Mature_435_residues
MNDNAADFDTTMPQGPFTSYADVLAHLDNLGLFHMELDLARIKRVLEELELTRPEYPVVQVVGTNGKGSTATFMASIATG
AGFSTGLFISPHFITPRERVLVDGEMLPEEDWLVLANDIMAAGGEVLTYFEFLTVLAALAFADYEVDLAVFEAGLGGTYD
ATTAIAADTVVFTPVALDHQQILGDTVEKIAADKAGAIRSNVPVVSARQTDAVLEILKNASNTAGAPFTVAAGPDSLPEE
ARPVLNGVHQLHNAALAVAALQTVMDEKALPRPTLIPWSDVVTLGIADAWIAGRMQHIAPAPDHPALILDGGHNLHGLRA
LQAALAAENIRPAAIIFGAMKDKNLEGIPDLLSALTDGPVLLPALTQNSRAATPQELAVRLGGAPDGPTETIPCASVREA
LDKAAAYAAENPVLICGSLYLLADFFTLHPEYLRP

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=418, Percent_Identity=29.4258373205742, Blast_Score=143, Evalue=3e-34,
Organism=Homo sapiens, GI66932990, Length=376, Percent_Identity=30.5851063829787, Blast_Score=143, Evalue=4e-34,
Organism=Escherichia coli, GI1788654, Length=186, Percent_Identity=39.7849462365591, Blast_Score=129, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI17553148, Length=315, Percent_Identity=30.1587301587302, Blast_Score=122, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI17553150, Length=315, Percent_Identity=30.1587301587302, Blast_Score=121, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI71984923, Length=315, Percent_Identity=30.1587301587302, Blast_Score=121, Evalue=6e-28,
Organism=Saccharomyces cerevisiae, GI6323760, Length=435, Percent_Identity=25.9770114942529, Blast_Score=124, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6324815, Length=283, Percent_Identity=30.0353356890459, Blast_Score=96, Evalue=8e-21,
Organism=Drosophila melanogaster, GI24641571, Length=401, Percent_Identity=26.1845386533666, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24581568, Length=195, Percent_Identity=31.2820512820513, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 45970; Mature: 45970

Theoretical pI: Translated: 4.30; Mature: 4.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDNAADFDTTMPQGPFTSYADVLAHLDNLGLFHMELDLARIKRVLEELELTRPEYPVVQ
CCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHCCCCCCCEEE
VVGTNGKGSTATFMASIATGAGFSTGLFISPHFITPRERVLVDGEMLPEEDWLVLANDIM
EEECCCCCCHHHHHHHHHCCCCCCCCEEECCCCCCCCHHEEECCCCCCCCCCEEEEHHHH
AAGGEVLTYFEFLTVLAALAFADYEVDLAVFEAGLGGTYDATTAIAADTVVFTPVALDHQ
HCCCHHHHHHHHHHHHHHHHHHCCCEEHEEEECCCCCCCCCHHHHHHCCEEEECEECCHH
QILGDTVEKIAADKAGAIRSNVPVVSARQTDAVLEILKNASNTAGAPFTVAAGPDSLPEE
HHHHHHHHHHHHHCCCCHHCCCCEEECCHHHHHHHHHHCCCCCCCCCEEEECCCCCCCHH
ARPVLNGVHQLHNAALAVAALQTVMDEKALPRPTLIPWSDVVTLGIADAWIAGRMQHIAP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
APDHPALILDGGHNLHGLRALQAALAAENIRPAAIIFGAMKDKNLEGIPDLLSALTDGPV
CCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHCCCCE
LLPALTQNSRAATPQELAVRLGGAPDGPTETIPCASVREALDKAAAYAAENPVLICGSLY
EEEEECCCCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEHHHH
LLADFFTLHPEYLRP
HHHHHHHCCHHHHCC
>Mature Secondary Structure
MNDNAADFDTTMPQGPFTSYADVLAHLDNLGLFHMELDLARIKRVLEELELTRPEYPVVQ
CCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHCCCCCCCEEE
VVGTNGKGSTATFMASIATGAGFSTGLFISPHFITPRERVLVDGEMLPEEDWLVLANDIM
EEECCCCCCHHHHHHHHHCCCCCCCCEEECCCCCCCCHHEEECCCCCCCCCCEEEEHHHH
AAGGEVLTYFEFLTVLAALAFADYEVDLAVFEAGLGGTYDATTAIAADTVVFTPVALDHQ
HCCCHHHHHHHHHHHHHHHHHHCCCEEHEEEECCCCCCCCCHHHHHHCCEEEECEECCHH
QILGDTVEKIAADKAGAIRSNVPVVSARQTDAVLEILKNASNTAGAPFTVAAGPDSLPEE
HHHHHHHHHHHHHCCCCHHCCCCEEECCHHHHHHHHHHCCCCCCCCCEEEECCCCCCCHH
ARPVLNGVHQLHNAALAVAALQTVMDEKALPRPTLIPWSDVVTLGIADAWIAGRMQHIAP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
APDHPALILDGGHNLHGLRALQAALAAENIRPAAIIFGAMKDKNLEGIPDLLSALTDGPV
CCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHCCCCE
LLPALTQNSRAATPQELAVRLGGAPDGPTETIPCASVREALDKAAAYAAENPVLICGSLY
EEEEECCCCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEHHHH
LLADFFTLHPEYLRP
HHHHHHHCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]