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The map label for this gene is ntrX [H]

Identifier: 78358043

GI number: 78358043

Start: 2988666

End: 2990063

Strand: Direct

Name: ntrX [H]

Synonym: Dde_3003

Alternate gene names: 78358043

Gene position: 2988666-2990063 (Clockwise)

Preceding gene: 78358042

Following gene: 78358044

Centisome position: 80.12

GC content: 57.8

Gene sequence:

>1398_bases
ATGAACGCGCGCGTTCTTATTGTGGATGACGAAGACGGCATCCGCTTTTCCCTGCGGGGTATTCTGGAAGATGAAGGCTA
TGAGGTAGCGGAGGCGGAAACCGGTGAAGAAGGCCTGAAACTGGCCACGGAAACGCCCCCCGACCTTATATTTCTGGATA
TATGGCTGCCGGGCATGGACGGACTGGAAGTGCTGGACAATCTGCGTACGGCCGATGCCTCTTTGCCGGTGATCATGATT
TCCGGTCACGGCAACATCGAAACCGCGGTCAACGCCATAAAAAAAGGCGCCTACGACTTCATCGAAAAACCCCTCTCGCT
GGAAAAAGTCGTCATTTCGGCCAACAAGGCGCTGGAGTATTACCGCCTGCGCAGAGAGAATCAGGCCCTGCGCTCGCGGC
TCACCCAGTCGACCACCGTGGAAATGACCGGAAACTCCGCTTCCATTGTGGAACTGCGCGGCCAGATAGGCCGGGTGGCC
CCCACGGACGCATGGGTGCTCATCACGGGAGAAAACGGCACGGGCAAGGAAATAGCGGCACGCTCCATTCACGCAAGCTC
GCGCAGAAAAGATAAACCGCTGGTGGCGGTCAACTGCGCCGCCATACCCGAAGAGCTTATCGAAAGCGAACTCTTCGGAC
ACGAAAAAGGCGCCTTCACCGGTGCCGAAACTGCCAAGACCGGCAAATTTGAAATGGCTCATCAGGGCACGCTGTTTCTG
GACGAAATTGCAGACATGAGCCTGAAAACGCAGGCAAAGATTCTGCGTATTCTGCAGGAGCAGAGCTTTGAGCGGGTGGG
CGGCCATAAAACGGTAAAGGTCGATGTGCGCGTTATCGCGGCAACCAATAAAAGTCTGGAAGATGAAATAGCCGCAGGCC
GGTTCCGCGAAGACCTGTATTACCGCCTGCGGGTCATCCCCCTTGTGGTGCCGCCGCTGCGCCAGCGCGGCGATGACGTT
ATGCTGCTCATCGGTGAATTCATGAACAGAATGACCGCGGAACACGGTTTCCGCCCTGTCACGTTTGCACCGGATGCGTG
TTCCGCCCTGCTGTGCTACCACTGGCCGGGCAACGTGCGTGAACTGAAAAATTTTGTGGAACGCATGCTCATCATGCACT
CCGGCCAGACGGTGACCGCCGCCATGCTGCCGCCCGAATACCGCTGCGCCGGTGCGGGCATAATGCCTCAGCCCTGTACC
GCAGGCCCGTCGGATGGTATGATCCCCGAAGGTGTCATGGATTTCAAGGCGGCGCGCACTGCCTTCGAGGCCCGCTTTCT
GGAGGCGAAACTGCAGGAGTTCGGCGGCAACATAACCCGCCTGGCCGAAGCCATCGGCCTTGAACGCAGCTACCTGTACC
GCAAACTGAAATCATACAACATTCAGACTCCGGAGTAG

Upstream 100 bases:

>100_bases
GTGCTGACGCCGTGCGTGCCTGCCTGGAACTTTTTGACGAGGGCTTTGCCTTTGACACTCCCGCGGAGCATCCCGCGGAC
AATGACGGAGACCTGACACC

Downstream 100 bases:

>100_bases
TGCACGCGGGTGCACCGCCCGCCGGAAGCAGGAGGCAGCATGTACAAGCTGGTACTTGTCCGCCACGGACAAAGCACATG
GAATCTGGAAAACCGGTTCA

Product: two component Fis family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 465; Mature: 465

Protein sequence:

>465_residues
MNARVLIVDDEDGIRFSLRGILEDEGYEVAEAETGEEGLKLATETPPDLIFLDIWLPGMDGLEVLDNLRTADASLPVIMI
SGHGNIETAVNAIKKGAYDFIEKPLSLEKVVISANKALEYYRLRRENQALRSRLTQSTTVEMTGNSASIVELRGQIGRVA
PTDAWVLITGENGTGKEIAARSIHASSRRKDKPLVAVNCAAIPEELIESELFGHEKGAFTGAETAKTGKFEMAHQGTLFL
DEIADMSLKTQAKILRILQEQSFERVGGHKTVKVDVRVIAATNKSLEDEIAAGRFREDLYYRLRVIPLVVPPLRQRGDDV
MLLIGEFMNRMTAEHGFRPVTFAPDACSALLCYHWPGNVRELKNFVERMLIMHSGQTVTAAMLPPEYRCAGAGIMPQPCT
AGPSDGMIPEGVMDFKAARTAFEARFLEAKLQEFGGNITRLAEAIGLERSYLYRKLKSYNIQTPE

Sequences:

>Translated_465_residues
MNARVLIVDDEDGIRFSLRGILEDEGYEVAEAETGEEGLKLATETPPDLIFLDIWLPGMDGLEVLDNLRTADASLPVIMI
SGHGNIETAVNAIKKGAYDFIEKPLSLEKVVISANKALEYYRLRRENQALRSRLTQSTTVEMTGNSASIVELRGQIGRVA
PTDAWVLITGENGTGKEIAARSIHASSRRKDKPLVAVNCAAIPEELIESELFGHEKGAFTGAETAKTGKFEMAHQGTLFL
DEIADMSLKTQAKILRILQEQSFERVGGHKTVKVDVRVIAATNKSLEDEIAAGRFREDLYYRLRVIPLVVPPLRQRGDDV
MLLIGEFMNRMTAEHGFRPVTFAPDACSALLCYHWPGNVRELKNFVERMLIMHSGQTVTAAMLPPEYRCAGAGIMPQPCT
AGPSDGMIPEGVMDFKAARTAFEARFLEAKLQEFGGNITRLAEAIGLERSYLYRKLKSYNIQTPE
>Mature_465_residues
MNARVLIVDDEDGIRFSLRGILEDEGYEVAEAETGEEGLKLATETPPDLIFLDIWLPGMDGLEVLDNLRTADASLPVIMI
SGHGNIETAVNAIKKGAYDFIEKPLSLEKVVISANKALEYYRLRRENQALRSRLTQSTTVEMTGNSASIVELRGQIGRVA
PTDAWVLITGENGTGKEIAARSIHASSRRKDKPLVAVNCAAIPEELIESELFGHEKGAFTGAETAKTGKFEMAHQGTLFL
DEIADMSLKTQAKILRILQEQSFERVGGHKTVKVDVRVIAATNKSLEDEIAAGRFREDLYYRLRVIPLVVPPLRQRGDDV
MLLIGEFMNRMTAEHGFRPVTFAPDACSALLCYHWPGNVRELKNFVERMLIMHSGQTVTAAMLPPEYRCAGAGIMPQPCT
AGPSDGMIPEGVMDFKAARTAFEARFLEAKLQEFGGNITRLAEAIGLERSYLYRKLKSYNIQTPE

Specific function: Member of the two-component regulatory system ntrY/ntrX involved in the activation of nitrogen assimilatory genes such as glnA. NtrX is probably phosphorylated by ntrY and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=460, Percent_Identity=42.8260869565217, Blast_Score=384, Evalue=1e-108,
Organism=Escherichia coli, GI1790437, Length=457, Percent_Identity=40.9190371991247, Blast_Score=335, Evalue=5e-93,
Organism=Escherichia coli, GI1790299, Length=467, Percent_Identity=38.5438972162741, Blast_Score=318, Evalue=6e-88,
Organism=Escherichia coli, GI1788905, Length=459, Percent_Identity=36.6013071895425, Blast_Score=291, Evalue=7e-80,
Organism=Escherichia coli, GI87082152, Length=326, Percent_Identity=42.638036809816, Blast_Score=249, Evalue=3e-67,
Organism=Escherichia coli, GI87082117, Length=259, Percent_Identity=49.034749034749, Blast_Score=249, Evalue=3e-67,
Organism=Escherichia coli, GI1789233, Length=328, Percent_Identity=40.8536585365854, Blast_Score=245, Evalue=5e-66,
Organism=Escherichia coli, GI1789087, Length=437, Percent_Identity=35.2402745995423, Blast_Score=243, Evalue=2e-65,
Organism=Escherichia coli, GI1786524, Length=347, Percent_Identity=40.0576368876081, Blast_Score=219, Evalue=3e-58,
Organism=Escherichia coli, GI87081872, Length=324, Percent_Identity=38.8888888888889, Blast_Score=209, Evalue=3e-55,
Organism=Escherichia coli, GI1787583, Length=326, Percent_Identity=38.9570552147239, Blast_Score=199, Evalue=4e-52,
Organism=Escherichia coli, GI87081858, Length=315, Percent_Identity=26.3492063492064, Blast_Score=128, Evalue=8e-31,
Organism=Escherichia coli, GI1789828, Length=242, Percent_Identity=34.7107438016529, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1786911, Length=103, Percent_Identity=43.6893203883495, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1787375, Length=108, Percent_Identity=32.4074074074074, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1786599, Length=113, Percent_Identity=35.3982300884956, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI87082012, Length=131, Percent_Identity=32.0610687022901, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1789809, Length=105, Percent_Identity=32.3809523809524, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790863, Length=114, Percent_Identity=35.0877192982456, Blast_Score=66, Evalue=6e-12,
Organism=Escherichia coli, GI1786784, Length=108, Percent_Identity=33.3333333333333, Blast_Score=66, Evalue=6e-12,
Organism=Escherichia coli, GI1788394, Length=125, Percent_Identity=32.8, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 51430; Mature: 51430

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNARVLIVDDEDGIRFSLRGILEDEGYEVAEAETGEEGLKLATETPPDLIFLDIWLPGMD
CCCEEEEEECCCCCEEEEEEEECCCCCEEECCCCCCCCCEEECCCCCCEEEEEEECCCCC
GLEVLDNLRTADASLPVIMISGHGNIETAVNAIKKGAYDFIEKPLSLEKVVISANKALEY
HHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHCHHHHHHCCCCHHHHHHHHHHHHHH
YRLRRENQALRSRLTQSTTVEMTGNSASIVELRGQIGRVAPTDAWVLITGENGTGKEIAA
HHHHHHHHHHHHHHCCCCEEEECCCCCEEEEEHHHCCCCCCCCEEEEEECCCCCCHHHHH
RSIHASSRRKDKPLVAVNCAAIPEELIESELFGHEKGAFTGAETAKTGKFEMAHQGTLFL
HHHHHHCCCCCCCEEEEEHHHCHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCCCEEH
DEIADMSLKTQAKILRILQEQSFERVGGHKTVKVDVRVIAATNKSLEDEIAAGRFREDLY
HHHHCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEEECCCCHHHHHHHHHHHHHHH
YRLRVIPLVVPPLRQRGDDVMLLIGEFMNRMTAEHGFRPVTFAPDACSALLCYHWPGNVR
HEEEEEEEECCHHHHCCCCEEEEHHHHHHHHHHHHCCCCEEECCCHHCEEEEECCCCCHH
ELKNFVERMLIMHSGQTVTAAMLPPEYRCAGAGIMPQPCTAGPSDGMIPEGVMDFKAART
HHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
AFEARFLEAKLQEFGGNITRLAEAIGLERSYLYRKLKSYNIQTPE
HHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNARVLIVDDEDGIRFSLRGILEDEGYEVAEAETGEEGLKLATETPPDLIFLDIWLPGMD
CCCEEEEEECCCCCEEEEEEEECCCCCEEECCCCCCCCCEEECCCCCCEEEEEEECCCCC
GLEVLDNLRTADASLPVIMISGHGNIETAVNAIKKGAYDFIEKPLSLEKVVISANKALEY
HHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHCHHHHHHCCCCHHHHHHHHHHHHHH
YRLRRENQALRSRLTQSTTVEMTGNSASIVELRGQIGRVAPTDAWVLITGENGTGKEIAA
HHHHHHHHHHHHHHCCCCEEEECCCCCEEEEEHHHCCCCCCCCEEEEEECCCCCCHHHHH
RSIHASSRRKDKPLVAVNCAAIPEELIESELFGHEKGAFTGAETAKTGKFEMAHQGTLFL
HHHHHHCCCCCCCEEEEEHHHCHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCCCEEH
DEIADMSLKTQAKILRILQEQSFERVGGHKTVKVDVRVIAATNKSLEDEIAAGRFREDLY
HHHHCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEEECCCCHHHHHHHHHHHHHHH
YRLRVIPLVVPPLRQRGDDVMLLIGEFMNRMTAEHGFRPVTFAPDACSALLCYHWPGNVR
HEEEEEEEECCHHHHCCCCEEEEHHHHHHHHHHHHCCCCEEECCCHHCEEEEECCCCCHH
ELKNFVERMLIMHSGQTVTAAMLPPEYRCAGAGIMPQPCTAGPSDGMIPEGVMDFKAART
HHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
AFEARFLEAKLQEFGGNITRLAEAIGLERSYLYRKLKSYNIQTPE
HHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1661370 [H]