Click here to switch to the map view.

The map label for this gene is tuf

Identifier: 78358029

GI number: 78358029

Start: 2969589

End: 2970782

Strand: Direct

Name: tuf

Synonym: Dde_2989

Alternate gene names: 78358029

Gene position: 2969589-2970782 (Clockwise)

Preceding gene: 78358028

Following gene: 304570604

Centisome position: 79.61

GC content: 56.87

Gene sequence:

>1194_bases
ATGGGTAAGGAAAAATTTGAACGTACTAAGCCGCATGTTAACATCGGCACCATCGGTCACATCGACCACGGCAAAACCAC
TCTGACCGCAGCAATCACCAAGATTGCCAGCCTCAAGATGGGTTCCAAAGCAGTTGCCTACGACGAAATCGACAAGGCTC
CTGAAGAAAAGGAACGCGGTATCACCATTGCTACCGCTCACGTGGAATACGAGACCACTCTGCGTCACTATGCCCACGTG
GACTGCCCCGGTCACGCTGACTACATCAAGAACATGATCACCGGTGCTGCTCAGATGGACGGCGCAATCATCGTTGTTGC
CGCCACCGACGGTCCCATGCCCCAGACCCGTGAGCACATCCTGCTTGCCCGTCAGGTCGGCGTGCCCAGCATCGTCGTGT
TCCTGAACAAGTGCGACATGGTTGACGACGAAGAACTGCTCGAACTGGTTGAGCTTGAAGTTCGCGAACTGCTGTCCAGC
TACGACTTCCCCGGCGACGACACCCCGGTTATCCGTGGTTCCGCCCTGAAGGCTCTGGAATGCGACAGCGCAGACGATGA
CGCTGCAAAGCCCATCCTTGAGCTGCTGGATGCATGCGACAGCTACATCCCCGAGCCCGAGCGCGACACCGACAAGCCCT
TCCTGATGCCCATCGAAGACGTGTTCTCGATCTCCGGCCGCGGTACCGTTGTTACCGGCCGTGTTGAGCGTGGCATTATC
AAGGTCGGCGAGCAGATCGAAATCGTTGGTATCAAAGATACCATGACCACCACCTGCACCGGTGTTGAAATGTTCCGCAA
GCTGCTTGACCAGGGTCAGGCCGGCGACAACGTGGGCGTGCTGCTGCGCGGTGTGAAGCGTGATGATGTTGAGCGTGGTC
AGGTTCTGGCTGCTCCCAAAAGCATCACTCCTCACAAGAAGTTCAAGGCAGAAGTGTACGTGCTCTCCAAGGAAGAAGGC
GGCCGTCATACTCCGTTCTTCTCCGGCTACCGTCCCCAGTTCTACTTCCGTACCACTGATATCACCGGTATCATCGGTCT
GGAAGAAGGCGTGGAAATGGTTATGCCCGGTGACAACGCCACCTTCATCGTGGAACTCATTCACCCCGTCGCCATGGAAC
AGGGCCTGCGCTTCGCTATCCGCGAAGGTGGCCGTACCGTAGGCGCAGGTGTTGTTTCCGAGATTCTGGAGTAA

Upstream 100 bases:

>100_bases
CGCGTCCTTGGTAAGGACGAGGTCAGCAGTTCAATTCTGCTCGTGGGCTCCATATTTTTTTTAGTTCCTAACTGAAAAAA
AACGCCAGGGAGAATTACTC

Downstream 100 bases:

>100_bases
TATCATGCGCGTGAATATTCAGCTGGCCTGCACCGAGTGCAAGCGGCGCAATTACGCGACTAATAAGAACAAGAAGAACA
CTACCGGCCGTCTGGAAGTG

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu

Number of amino acids: Translated: 397; Mature: 396

Protein sequence:

>397_residues
MGKEKFERTKPHVNIGTIGHIDHGKTTLTAAITKIASLKMGSKAVAYDEIDKAPEEKERGITIATAHVEYETTLRHYAHV
DCPGHADYIKNMITGAAQMDGAIIVVAATDGPMPQTREHILLARQVGVPSIVVFLNKCDMVDDEELLELVELEVRELLSS
YDFPGDDTPVIRGSALKALECDSADDDAAKPILELLDACDSYIPEPERDTDKPFLMPIEDVFSISGRGTVVTGRVERGII
KVGEQIEIVGIKDTMTTTCTGVEMFRKLLDQGQAGDNVGVLLRGVKRDDVERGQVLAAPKSITPHKKFKAEVYVLSKEEG
GRHTPFFSGYRPQFYFRTTDITGIIGLEEGVEMVMPGDNATFIVELIHPVAMEQGLRFAIREGGRTVGAGVVSEILE

Sequences:

>Translated_397_residues
MGKEKFERTKPHVNIGTIGHIDHGKTTLTAAITKIASLKMGSKAVAYDEIDKAPEEKERGITIATAHVEYETTLRHYAHV
DCPGHADYIKNMITGAAQMDGAIIVVAATDGPMPQTREHILLARQVGVPSIVVFLNKCDMVDDEELLELVELEVRELLSS
YDFPGDDTPVIRGSALKALECDSADDDAAKPILELLDACDSYIPEPERDTDKPFLMPIEDVFSISGRGTVVTGRVERGII
KVGEQIEIVGIKDTMTTTCTGVEMFRKLLDQGQAGDNVGVLLRGVKRDDVERGQVLAAPKSITPHKKFKAEVYVLSKEEG
GRHTPFFSGYRPQFYFRTTDITGIIGLEEGVEMVMPGDNATFIVELIHPVAMEQGLRFAIREGGRTVGAGVVSEILE
>Mature_396_residues
GKEKFERTKPHVNIGTIGHIDHGKTTLTAAITKIASLKMGSKAVAYDEIDKAPEEKERGITIATAHVEYETTLRHYAHVD
CPGHADYIKNMITGAAQMDGAIIVVAATDGPMPQTREHILLARQVGVPSIVVFLNKCDMVDDEELLELVELEVRELLSSY
DFPGDDTPVIRGSALKALECDSADDDAAKPILELLDACDSYIPEPERDTDKPFLMPIEDVFSISGRGTVVTGRVERGIIK
VGEQIEIVGIKDTMTTTCTGVEMFRKLLDQGQAGDNVGVLLRGVKRDDVERGQVLAAPKSITPHKKFKAEVYVLSKEEGG
RHTPFFSGYRPQFYFRTTDITGIIGLEEGVEMVMPGDNATFIVELIHPVAMEQGLRFAIREGGRTVGAGVVSEILE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily

Homologues:

Organism=Homo sapiens, GI34147630, Length=395, Percent_Identity=56.7088607594937, Blast_Score=465, Evalue=1e-131,
Organism=Homo sapiens, GI4503471, Length=458, Percent_Identity=32.0960698689956, Blast_Score=175, Evalue=7e-44,
Organism=Homo sapiens, GI5729864, Length=441, Percent_Identity=30.6122448979592, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI223555963, Length=441, Percent_Identity=30.6122448979592, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI4503475, Length=463, Percent_Identity=31.1015118790497, Blast_Score=174, Evalue=2e-43,
Organism=Homo sapiens, GI54607086, Length=319, Percent_Identity=30.0940438871473, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI194018522, Length=337, Percent_Identity=27.0029673590504, Blast_Score=115, Evalue=8e-26,
Organism=Homo sapiens, GI194018520, Length=337, Percent_Identity=27.0029673590504, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI194097354, Length=337, Percent_Identity=27.0029673590504, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI46094014, Length=329, Percent_Identity=28.2674772036474, Blast_Score=113, Evalue=4e-25,
Organism=Homo sapiens, GI4503507, Length=278, Percent_Identity=28.0575539568345, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI82546879, Length=439, Percent_Identity=24.8291571753986, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI157426893, Length=301, Percent_Identity=24.5847176079734, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1790412, Length=396, Percent_Identity=78.2828282828283, Blast_Score=618, Evalue=1e-178,
Organism=Escherichia coli, GI1789737, Length=396, Percent_Identity=78.2828282828283, Blast_Score=617, Evalue=1e-178,
Organism=Escherichia coli, GI2367247, Length=340, Percent_Identity=29.4117647058824, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI48994988, Length=239, Percent_Identity=33.4728033472803, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1789108, Length=333, Percent_Identity=28.2282282282282, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1788922, Length=333, Percent_Identity=26.1261261261261, Blast_Score=62, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI17556456, Length=405, Percent_Identity=53.8271604938272, Blast_Score=392, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI25141371, Length=388, Percent_Identity=47.1649484536082, Blast_Score=359, Evalue=1e-99,
Organism=Caenorhabditis elegans, GI17552884, Length=459, Percent_Identity=32.244008714597, Blast_Score=171, Evalue=5e-43,
Organism=Caenorhabditis elegans, GI17569207, Length=459, Percent_Identity=32.244008714597, Blast_Score=171, Evalue=5e-43,
Organism=Caenorhabditis elegans, GI32566303, Length=440, Percent_Identity=30.4545454545455, Blast_Score=151, Evalue=5e-37,
Organism=Caenorhabditis elegans, GI115532067, Length=435, Percent_Identity=27.1264367816092, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI115532065, Length=435, Percent_Identity=27.1264367816092, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17506081, Length=275, Percent_Identity=30.5454545454545, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI32566629, Length=454, Percent_Identity=24.6696035242291, Blast_Score=122, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI32566301, Length=154, Percent_Identity=40.9090909090909, Blast_Score=95, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17509919, Length=282, Percent_Identity=25.886524822695, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17557151, Length=239, Percent_Identity=28.0334728033473, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6324761, Length=397, Percent_Identity=64.9874055415617, Blast_Score=522, Evalue=1e-149,
Organism=Saccharomyces cerevisiae, GI6325337, Length=451, Percent_Identity=31.4855875831486, Blast_Score=169, Evalue=7e-43,
Organism=Saccharomyces cerevisiae, GI6319594, Length=451, Percent_Identity=31.4855875831486, Blast_Score=169, Evalue=7e-43,
Organism=Saccharomyces cerevisiae, GI6322937, Length=458, Percent_Identity=24.235807860262, Blast_Score=98, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6320377, Length=342, Percent_Identity=29.2397660818713, Blast_Score=90, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6320863, Length=283, Percent_Identity=25.0883392226148, Blast_Score=84, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6323320, Length=257, Percent_Identity=26.4591439688716, Blast_Score=70, Evalue=8e-13,
Organism=Drosophila melanogaster, GI281363316, Length=394, Percent_Identity=57.3604060913706, Blast_Score=469, Evalue=1e-132,
Organism=Drosophila melanogaster, GI17864358, Length=394, Percent_Identity=57.3604060913706, Blast_Score=469, Evalue=1e-132,
Organism=Drosophila melanogaster, GI19921738, Length=389, Percent_Identity=51.6709511568123, Blast_Score=407, Evalue=1e-114,
Organism=Drosophila melanogaster, GI45553807, Length=456, Percent_Identity=30.2631578947368, Blast_Score=171, Evalue=6e-43,
Organism=Drosophila melanogaster, GI45553816, Length=456, Percent_Identity=30.2631578947368, Blast_Score=171, Evalue=6e-43,
Organism=Drosophila melanogaster, GI24651721, Length=456, Percent_Identity=30.2631578947368, Blast_Score=171, Evalue=6e-43,
Organism=Drosophila melanogaster, GI17864154, Length=456, Percent_Identity=30.2631578947368, Blast_Score=171, Evalue=6e-43,
Organism=Drosophila melanogaster, GI24652838, Length=458, Percent_Identity=31.6593886462882, Blast_Score=167, Evalue=8e-42,
Organism=Drosophila melanogaster, GI17137572, Length=458, Percent_Identity=31.6593886462882, Blast_Score=167, Evalue=8e-42,
Organism=Drosophila melanogaster, GI45550900, Length=438, Percent_Identity=27.3972602739726, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI19922690, Length=306, Percent_Identity=30.3921568627451, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI17137380, Length=348, Percent_Identity=28.448275862069, Blast_Score=118, Evalue=6e-27,
Organism=Drosophila melanogaster, GI24647046, Length=269, Percent_Identity=26.7657992565056, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24647048, Length=269, Percent_Identity=26.7657992565056, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): EFTU_DESDG (Q30X13)

Other databases:

- EMBL:   CP000112
- RefSeq:   YP_389478.1
- HSSP:   P0A6N1
- ProteinModelPortal:   Q30X13
- SMR:   Q30X13
- STRING:   Q30X13
- GeneID:   3757998
- GenomeReviews:   CP000112_GR
- KEGG:   dde:Dde_2989
- NMPDR:   fig|207559.3.peg.3078
- eggNOG:   COG0050
- HOGENOM:   HBG307581
- OMA:   RDKPFLM
- ProtClustDB:   PRK12735
- BioCyc:   DDES207559:DDE_2989-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00118_B
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000
- PANTHER:   PTHR23115:SF31
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00485
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3; SSF50465 Elong_init_C; SSF50447 Translat_factor

EC number: 3.6.5.3

Molecular weight: Translated: 43435; Mature: 43304

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKEKFERTKPHVNIGTIGHIDHGKTTLTAAITKIASLKMGSKAVAYDEIDKAPEEKERG
CCCHHHHHCCCCEEECEEECCCCCCHHHHHHHHHHHHHCCCCCEEEHHHHHCCCCHHHCC
ITIATAHVEYETTLRHYAHVDCPGHADYIKNMITGAAQMDGAIIVVAATDGPMPQTREHI
EEEEEEEEEHHHHHHHHEECCCCCHHHHHHHHHHCHHHCCCEEEEEEECCCCCCCCHHHH
LLARQVGVPSIVVFLNKCDMVDDEELLELVELEVRELLSSYDFPGDDTPVIRGSALKALE
HHHHHCCCCEEEEEEHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCEEEEE
CDSADDDAAKPILELLDACDSYIPEPERDTDKPFLMPIEDVFSISGRGTVVTGRVERGII
CCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHCCCCCCEEEECHHHHCHH
KVGEQIEIVGIKDTMTTTCTGVEMFRKLLDQGQAGDNVGVLLRGVKRDDVERGQVLAAPK
CCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCEEEECCC
SITPHKKFKAEVYVLSKEEGGRHTPFFSGYRPQFYFRTTDITGIIGLEEGVEMVMPGDNA
CCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCEEEECCCCC
TFIVELIHPVAMEQGLRFAIREGGRTVGAGVVSEILE
EEHHHHHHHHHHHHCCCHHHHCCCCEEHHHHHHHHHC
>Mature Secondary Structure 
GKEKFERTKPHVNIGTIGHIDHGKTTLTAAITKIASLKMGSKAVAYDEIDKAPEEKERG
CCHHHHHCCCCEEECEEECCCCCCHHHHHHHHHHHHHCCCCCEEEHHHHHCCCCHHHCC
ITIATAHVEYETTLRHYAHVDCPGHADYIKNMITGAAQMDGAIIVVAATDGPMPQTREHI
EEEEEEEEEHHHHHHHHEECCCCCHHHHHHHHHHCHHHCCCEEEEEEECCCCCCCCHHHH
LLARQVGVPSIVVFLNKCDMVDDEELLELVELEVRELLSSYDFPGDDTPVIRGSALKALE
HHHHHCCCCEEEEEEHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCEEEEE
CDSADDDAAKPILELLDACDSYIPEPERDTDKPFLMPIEDVFSISGRGTVVTGRVERGII
CCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHCCCCCCEEEECHHHHCHH
KVGEQIEIVGIKDTMTTTCTGVEMFRKLLDQGQAGDNVGVLLRGVKRDDVERGQVLAAPK
CCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCEEEECCC
SITPHKKFKAEVYVLSKEEGGRHTPFFSGYRPQFYFRTTDITGIIGLEEGVEMVMPGDNA
CCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCEEEECCCCC
TFIVELIHPVAMEQGLRFAIREGGRTVGAGVVSEILE
EEHHHHHHHHHHHHCCCHHHHCCCCEEHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA