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The map label for this gene is rlmN

Identifier: 78358007

GI number: 78358007

Start: 2950993

End: 2952069

Strand: Direct

Name: rlmN

Synonym: Dde_2967

Alternate gene names: 78358007

Gene position: 2950993-2952069 (Clockwise)

Preceding gene: 78358006

Following gene: 78358015

Centisome position: 79.11

GC content: 56.55

Gene sequence:

>1077_bases
ATGGTCGATATTCTGAACCTGACATTTGAAGAGCTTGAAACTTTTCTGGTGGAAAAGCTCGGCGAAAAAAAATTCCGCGC
CCGCCAGATATGGCAGTGGCTGTGGAACAAATATGTGCGCGATTTTGACCAGATGACCAATGTATCGAAACAGACCCGCG
CACAGCTGAAAGAACACGCCCGCATTTTCTGGCCAGAGGTGGTCACAACCAGCAAAAGTCAGGACGGCACCACAAAATTT
CTGCTGCGTCTTGCCGACGGCGCACTGGTGGAAACAGTGCTCATACCCGGTTCACAGGGCAGGATTACCCAGTGTCTTTC
CTGTCAGGTGGGCTGCGCCATGGGGTGCACCTTTTGCGCCACCGGCACGCTGGGTTTTGAACGCAACATGACCATGTCTG
AAATACTGGGGCAGGTGCTGGTGGCACGCGAATATCTTAACGACGTCGCCGAACGGCCCATCCTGCGCAATCTTGTCTTT
ATGGGCATGGGTGAACCGCTGCTCAATCTGGACGAAATCATGCGCAGCCTGCACACGCTTAACAGCGAGCTGGGACTGCA
GTTTTCTCCGCGGCGCATCACGGTTTCCACCTGCGGGGTAAACCCCGAAGGGCTGCGCAGGCTCGGCGAAAGCGGGCTTG
CCTATCTGGCTGTATCACTGCACGCGCCCACGCAGGAACTGCGGCGCACCATCATGCCCAAAGCCGCCCGCTGGGAACTG
AATGACTTCATTGAAGCCCTGCAGAGCTACCCGCTGAAAACGCGTGAACGCATAACCTTTGAATATCTGCTGCTGGGCGG
GGTCAACGACTCGCTGGAGCACGCAAAACAGCTTGTCCGGCTTGTTTCACGCACCAAGGCAAAGCTCAATCTCATTGTGT
ACAACCCGTCGGGCGACGAGGCAGACCCTTATGCAGCCCCCACGGAAGAACGCATTCTGGCTTTTGAGCAATACCTGTGG
TCAAAGCATGTCACGGCCATCATACGCAAAAGCAAAGGGGCGGACATCAAAGCCGCCTGCGGTCAGCTGAAAGCCGCCGA
AACCGGCGGCACCCCCTGCTGCGCGCAGAACGACTGA

Upstream 100 bases:

>100_bases
CCGTTTTTACCGCGGGCTGCTGCCTTTCCCGTCGTGCCGCAGCGCGCCGGACCGCGGCAGCGCCTGTGCGTCAGCCATTC
CACTCACCGGAGAAACAGTC

Downstream 100 bases:

>100_bases
CCCGATGCCGCCCTCTGCGGCACAGCCATAAAAAAGACTCCGTACCGGCCACCCTGCGGTGCTCCGGTACGGAGTCTTCA
TATTTCACAGACAGCCGCAG

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MVDILNLTFEELETFLVEKLGEKKFRARQIWQWLWNKYVRDFDQMTNVSKQTRAQLKEHARIFWPEVVTTSKSQDGTTKF
LLRLADGALVETVLIPGSQGRITQCLSCQVGCAMGCTFCATGTLGFERNMTMSEILGQVLVAREYLNDVAERPILRNLVF
MGMGEPLLNLDEIMRSLHTLNSELGLQFSPRRITVSTCGVNPEGLRRLGESGLAYLAVSLHAPTQELRRTIMPKAARWEL
NDFIEALQSYPLKTRERITFEYLLLGGVNDSLEHAKQLVRLVSRTKAKLNLIVYNPSGDEADPYAAPTEERILAFEQYLW
SKHVTAIIRKSKGADIKAACGQLKAAETGGTPCCAQND

Sequences:

>Translated_358_residues
MVDILNLTFEELETFLVEKLGEKKFRARQIWQWLWNKYVRDFDQMTNVSKQTRAQLKEHARIFWPEVVTTSKSQDGTTKF
LLRLADGALVETVLIPGSQGRITQCLSCQVGCAMGCTFCATGTLGFERNMTMSEILGQVLVAREYLNDVAERPILRNLVF
MGMGEPLLNLDEIMRSLHTLNSELGLQFSPRRITVSTCGVNPEGLRRLGESGLAYLAVSLHAPTQELRRTIMPKAARWEL
NDFIEALQSYPLKTRERITFEYLLLGGVNDSLEHAKQLVRLVSRTKAKLNLIVYNPSGDEADPYAAPTEERILAFEQYLW
SKHVTAIIRKSKGADIKAACGQLKAAETGGTPCCAQND
>Mature_358_residues
MVDILNLTFEELETFLVEKLGEKKFRARQIWQWLWNKYVRDFDQMTNVSKQTRAQLKEHARIFWPEVVTTSKSQDGTTKF
LLRLADGALVETVLIPGSQGRITQCLSCQVGCAMGCTFCATGTLGFERNMTMSEILGQVLVAREYLNDVAERPILRNLVF
MGMGEPLLNLDEIMRSLHTLNSELGLQFSPRRITVSTCGVNPEGLRRLGESGLAYLAVSLHAPTQELRRTIMPKAARWEL
NDFIEALQSYPLKTRERITFEYLLLGGVNDSLEHAKQLVRLVSRTKAKLNLIVYNPSGDEADPYAAPTEERILAFEQYLW
SKHVTAIIRKSKGADIKAACGQLKAAETGGTPCCAQND

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=348, Percent_Identity=42.2413793103448, Blast_Score=265, Evalue=3e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_DESDG (Q30X35)

Other databases:

- EMBL:   CP000112
- RefSeq:   YP_389456.1
- ProteinModelPortal:   Q30X35
- STRING:   Q30X35
- GeneID:   3757972
- GenomeReviews:   CP000112_GR
- KEGG:   dde:Dde_2967
- NMPDR:   fig|207559.3.peg.2989
- eggNOG:   COG0820
- HOGENOM:   HBG335345
- OMA:   TFCSTAQ
- ProtClustDB:   PRK14460
- BioCyc:   DDES207559:DDE_2967-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- SMART:   SM00729
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 40277; Mature: 40277

Theoretical pI: Translated: 7.93; Mature: 7.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDILNLTFEELETFLVEKLGEKKFRARQIWQWLWNKYVRDFDQMTNVSKQTRAQLKEHA
CCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
RIFWPEVVTTSKSQDGTTKFLLRLADGALVETVLIPGSQGRITQCLSCQVGCAMGCTFCA
HEECHHHHCCCCCCCCHHHHHHHHHCCHHEEEEECCCCCCCHHHHHHHHHHHHHCHHHHH
TGTLGFERNMTMSEILGQVLVAREYLNDVAERPILRNLVFMGMGEPLLNLDEIMRSLHTL
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCHHHHHHHHHHH
NSELGLQFSPRRITVSTCGVNPEGLRRLGESGLAYLAVSLHAPTQELRRTIMPKAARWEL
HHHHCCEECCCEEEEEECCCCHHHHHHHHCCCCEEEEEEECCCHHHHHHHHCCCHHCCCH
NDFIEALQSYPLKTRERITFEYLLLGGVNDSLEHAKQLVRLVSRTKAKLNLIVYNPSGDE
HHHHHHHHHCCCCHHHHHEEEEEEECCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCC
ADPYAAPTEERILAFEQYLWSKHVTAIIRKSKGADIKAACGQLKAAETGGTPCCAQND
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MVDILNLTFEELETFLVEKLGEKKFRARQIWQWLWNKYVRDFDQMTNVSKQTRAQLKEHA
CCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
RIFWPEVVTTSKSQDGTTKFLLRLADGALVETVLIPGSQGRITQCLSCQVGCAMGCTFCA
HEECHHHHCCCCCCCCHHHHHHHHHCCHHEEEEECCCCCCCHHHHHHHHHHHHHCHHHHH
TGTLGFERNMTMSEILGQVLVAREYLNDVAERPILRNLVFMGMGEPLLNLDEIMRSLHTL
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCHHHHHHHHHHH
NSELGLQFSPRRITVSTCGVNPEGLRRLGESGLAYLAVSLHAPTQELRRTIMPKAARWEL
HHHHCCEECCCEEEEEECCCCHHHHHHHHCCCCEEEEEEECCCHHHHHHHHCCCHHCCCH
NDFIEALQSYPLKTRERITFEYLLLGGVNDSLEHAKQLVRLVSRTKAKLNLIVYNPSGDE
HHHHHHHHHCCCCHHHHHEEEEEEECCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCC
ADPYAAPTEERILAFEQYLWSKHVTAIIRKSKGADIKAACGQLKAAETGGTPCCAQND
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA