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The map label for this gene is hmeA [H]

Identifier: 78357974

GI number: 78357974

Start: 2915320

End: 2916087

Strand: Direct

Name: hmeA [H]

Synonym: Dde_2934

Alternate gene names: 78357974

Gene position: 2915320-2916087 (Clockwise)

Preceding gene: 78357973

Following gene: 78357975

Centisome position: 78.15

GC content: 56.9

Gene sequence:

>768_bases
ATGTCCTCTAAAAAGGAATTCAAAATCAAATGGGGTATGGCAATAGACCTCGATAAATGCACGGGCTGCGGCGCGTGCAT
GGTCGCCTGCCAGGCAGAAAACAACATTGCCCCTGCTCCGGACGCAACCAACAAGCTGAAAACGCTCAACTGGCTTGTGG
TGTACGAGCTGTCCAACAAGAAACCGTTTCCCGACCACGATGTGGCATACCTGCCGCGGCCCTGCCAGCAGTGCGGCAAC
CCGCCCTGCGTTTCGGTATGTCCTGTTATCGCCACCGACAAAAACGAAGAAGGCGGCATAGTCAGTCAGGTAACCCCCCG
CTGTATCGGCTGCCGGTACTGCATGGCCGCCTGCCCCTACCATGCCCGCTACTTCAACTGGTACGATCCCATCTGGCCGG
AAGGCATGGAAAAAACACTGACGCCCGATGTGTCTGTAAGACCCCGCGGCGTAGTGGAAAAATGCACCTTCTGCCATCAT
CGCATGATGGCTGCCAGAGACAAGGCAAGAGTGGAAGGGCGTGATCCCGATGCACTGGAAGATGGCGAATACATCACCTC
GTGCACCGAGGCATGCCCCAACGGTGCCATAGTGTTCGGCGATCTGCTGAACCCTGACCACAAGGTGTATGAACTGTCGC
GCAGCAAGTACGCCTTCCGGCTGCTTGAACGTCTGGGTGCCGACACGCAGGTGTACTACCTCAGCCGTCGCGAATGGGTC
CGCCGTCTTGGCGACAACTATCTTGAACACGAAAAGGTCAAGGGGTAG

Upstream 100 bases:

>100_bases
CGTCATGCATCTGCTTACAGCAGGATTCGAGCCGGTTACCGGACTTTCTGTCTGGAACCGCGCCGGCGTGAACATTGCCA
AAGCATAAGGGAAGTACGTC

Downstream 100 bases:

>100_bases
CTCTCATGGAAAAGAACTACGATCTTCCCGTTGATCACGAGCTGTTCCCCGAGGGAACCAAACGCTGTTCCTTCAGCAAG
TTCTCCGTATGGATGGCTGT

Product: molybdopterin oxidoreductase, iron-sulfur cluster-binding subunit

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: Hme subunit A [H]

Number of amino acids: Translated: 255; Mature: 254

Protein sequence:

>255_residues
MSSKKEFKIKWGMAIDLDKCTGCGACMVACQAENNIAPAPDATNKLKTLNWLVVYELSNKKPFPDHDVAYLPRPCQQCGN
PPCVSVCPVIATDKNEEGGIVSQVTPRCIGCRYCMAACPYHARYFNWYDPIWPEGMEKTLTPDVSVRPRGVVEKCTFCHH
RMMAARDKARVEGRDPDALEDGEYITSCTEACPNGAIVFGDLLNPDHKVYELSRSKYAFRLLERLGADTQVYYLSRREWV
RRLGDNYLEHEKVKG

Sequences:

>Translated_255_residues
MSSKKEFKIKWGMAIDLDKCTGCGACMVACQAENNIAPAPDATNKLKTLNWLVVYELSNKKPFPDHDVAYLPRPCQQCGN
PPCVSVCPVIATDKNEEGGIVSQVTPRCIGCRYCMAACPYHARYFNWYDPIWPEGMEKTLTPDVSVRPRGVVEKCTFCHH
RMMAARDKARVEGRDPDALEDGEYITSCTEACPNGAIVFGDLLNPDHKVYELSRSKYAFRLLERLGADTQVYYLSRREWV
RRLGDNYLEHEKVKG
>Mature_254_residues
SSKKEFKIKWGMAIDLDKCTGCGACMVACQAENNIAPAPDATNKLKTLNWLVVYELSNKKPFPDHDVAYLPRPCQQCGNP
PCVSVCPVIATDKNEEGGIVSQVTPRCIGCRYCMAACPYHARYFNWYDPIWPEGMEKTLTPDVSVRPRGVVEKCTFCHHR
MMAARDKARVEGRDPDALEDGEYITSCTEACPNGAIVFGDLLNPDHKVYELSRSKYAFRLLERLGADTQVYYLSRREWVR
RLGDNYLEHEKVKG

Specific function: Has menaquinol-oxidizing activity. HmeA, hmeB and hmeE subunits may together catalyze electron transfer from menaquinol to cytochrome c [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Extracellular side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Escherichia coli, GI2367345, Length=224, Percent_Identity=30.8035714285714, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI1789370, Length=248, Percent_Identity=28.6290322580645, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI226510944, Length=252, Percent_Identity=26.5873015873016, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1787740, Length=162, Percent_Identity=31.4814814814815, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI1787122, Length=200, Percent_Identity=28.5, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1787872, Length=200, Percent_Identity=28.5, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1787478, Length=104, Percent_Identity=37.5, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1790326, Length=205, Percent_Identity=28.2926829268293, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1787749, Length=208, Percent_Identity=26.9230769230769, Blast_Score=68, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006311 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 28839; Mature: 28708

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

6.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
9.0 %Cys+Met (Translated Protein)
6.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
8.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSKKEFKIKWGMAIDLDKCTGCGACMVACQAENNIAPAPDATNKLKTLNWLVVYELSNK
CCCCCCEEEEECEEEEHHHCCCCCEEEEEEECCCCCCCCCCCCCHHEEEEEEEEEEECCC
KPFPDHDVAYLPRPCQQCGNPPCVSVCPVIATDKNEEGGIVSQVTPRCIGCRYCMAACPY
CCCCCCCCCCCCCHHHHCCCCCCCEECCEEECCCCCCCCCHHHCCHHHHHHHHHHHHCCH
HARYFNWYDPIWPEGMEKTLTPDVSVRPRGVVEKCTFCHHRMMAARDKARVEGRDPDALE
HHHHCCCCCCCCCCCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
DGEYITSCTEACPNGAIVFGDLLNPDHKVYELSRSKYAFRLLERLGADTQVYYLSRREWV
CCHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHH
RRLGDNYLEHEKVKG
HHHCCHHHHHHHCCC
>Mature Secondary Structure 
SSKKEFKIKWGMAIDLDKCTGCGACMVACQAENNIAPAPDATNKLKTLNWLVVYELSNK
CCCCCEEEEECEEEEHHHCCCCCEEEEEEECCCCCCCCCCCCCHHEEEEEEEEEEECCC
KPFPDHDVAYLPRPCQQCGNPPCVSVCPVIATDKNEEGGIVSQVTPRCIGCRYCMAACPY
CCCCCCCCCCCCCHHHHCCCCCCCEECCEEECCCCCCCCCHHHCCHHHHHHHHHHHHCCH
HARYFNWYDPIWPEGMEKTLTPDVSVRPRGVVEKCTFCHHRMMAARDKARVEGRDPDALE
HHHHCCCCCCCCCCCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
DGEYITSCTEACPNGAIVFGDLLNPDHKVYELSRSKYAFRLLERLGADTQVYYLSRREWV
CCHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHH
RRLGDNYLEHEKVKG
HHHCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9389475; 11952791 [H]