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The map label for this gene is asnB [H]

Identifier: 78357932

GI number: 78357932

Start: 2878891

End: 2880747

Strand: Reverse

Name: asnB [H]

Synonym: Dde_2891

Alternate gene names: 78357932

Gene position: 2880747-2878891 (Counterclockwise)

Preceding gene: 78357933

Following gene: 78357931

Centisome position: 77.23

GC content: 45.88

Gene sequence:

>1857_bases
ATGTGTGGAATTTTTGGAATAATATCTCAACTTGTTCCCCCGTCGAAACGTGAACTGGAAACGTCATGTGAGCTGTTAGC
TCACCGCGGTCCAGACGGATATGGGTACTTTATAAAAGGCCCTGTAGGCTTTGCGCATCGTCGCTTGTCGATTATTGATA
TCGCACAGGGTTCTCAGCCTATGAGTACGCAAGAGGGCGAGCTGACCATTACTTACAATGGTGAAATATACAATTTCCGC
CACATCAAAGCTTCTCTCGTAGCGAAGGGATATAGCTTTCAAACAAACTCTGATACTGAAGTTATCCTTGCCGCTTACAA
AGAATGGGGAACAGAATGCGTCCAGCATCTTAGGGGCATGTTTGCTTTTGCAATTGCTGATCACATTCGACAAACTGTTT
TTTTAGCACGAGATCCGTTTGGCATAAAACCTCTTGTCTATCGTGAATCTTCTTTAGGCTTTGCCTTTTCCTCTGAACTG
CCCGCGTTGGTCCGATTGAAAAAATGGCCTTCGGTCACAGTTTCTCCCAAAGCTGTTTCTCTTTTTTTTCGTCATCAATA
TATTCCAGCGCCTTATACCATTTATAATGAGACTCAAAAGCTACCCCCAGCCCACGCCATGCTGGTGGACTTTAATGGGT
GCATTCAACAAAAATGGCGCTATTGGACCCCCGCATTTCACCCCAGCGTTACTTATTCATTGCAAGATGCAATCAAAGAC
GCCCATAATTCTGTGACAGAGTCGATAGACGCGCATACTCTGTCAGACGTTCCTATCGGTGTCTTTCTATCTGGGGGCAT
AGATTCAACATTAGTCGCAGCCAGACTTGCGCAAAGGCACTCTCGATCGATACCAGCCTTTACAATAGGATATAAAGAGA
GTGACTTTTCAGAACTCCCTTATGCTCAGCATGCAGCAGACAAGCTAGGGCTCAAGCTGTACGCTCAAACGCTTGATGAA
AAAGCGTTGAAGTTCGCGCCTGAAATTTTATCTCATTATGGAGAGCCCTTTGGAGACAGCTCCGTAATTCCAACGTGGCA
TGTATGTAAACTTGCAAGTGAGCATGTTCCAGTCTGTCTTTCTGGAGATGGCGCAGATGAGTTGTTTGGTGGGTATCAGA
AGTATCAAATCTGGTCCGAGAGCACACCGTGGCGACAGTTGCAACTGGCAGGGAGTGCAATTGCAAAAAGAAAATTGCGT
ACTGCATGGTGGATGTTGAGGCGGGCTTCACGTTCTTTTTGTGATGACAAGGCGTTTTGGCTGGACCACGTTCAGTATTA
TCCTCCACAAGGGATCCGGCAGCTAACGCATCCCGATATGCACCCATATGCCAACGCGTTTGCTCCTGCTTTTGATGAGC
CATTTCTTAACATCGATAAATTAGGGCGCTTCGAATACGCTCAACGGATGGATTTACTATCCTATCTGCCAGACTGTCTT
CTTGCAAAAGTAGACTTGGCAAGCATGGCGCATGGGCTTGAAGTAAGACCTGCCTTACTAGATAAGTATGTTTTTCAACT
AGCAGCTACTCTTCCTACGGATGTAAAACTACATCCACAAAAGGGAGGGAAAGTCGTGCTGAAGGAATTACTGTTGCGCC
AAGGATTTTCCGAAGAGTTTGTCTATCGCCCGAAACAAGGCTTTGCGCTTCCGCTTCGGCATTGGTTCATCTCTGGACAG
CCAGCAAATAATATGCTGTGTGAGTTGGTTTCCTCTTATAAACGTGAACTCTCGGAACACTTAACTGTCTCAGATATTGA
AAGAATGTTAACGGCCCATTCGCCTGCGTGCGATTATTCTGGGCCGCTTTGGCTTGCCTTTGCTTTTACCTGTTGGGTCG
CAAATACTTCGAAATAA

Upstream 100 bases:

>100_bases
GTGCTGGCCAAAGTGAAAAATTATAAATCTCCGATTCATTTCTTAGCACCTTTTATTTACGCAACAGGACGTGTAGAGAA
CTAAGGATGAGAAGATAAAA

Downstream 100 bases:

>100_bases
AATGCGCAGTAAACCACGTATTCTTATAGCCCTAAGCTCTGTCGGACTCGGAGGCGCTGAGCGTCAAGGGTTAATGTTCG
CAGAATATTTAAAATGCAAG

Product: asparagine synthase, glutamine-hydrolyzing

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 618; Mature: 618

Protein sequence:

>618_residues
MCGIFGIISQLVPPSKRELETSCELLAHRGPDGYGYFIKGPVGFAHRRLSIIDIAQGSQPMSTQEGELTITYNGEIYNFR
HIKASLVAKGYSFQTNSDTEVILAAYKEWGTECVQHLRGMFAFAIADHIRQTVFLARDPFGIKPLVYRESSLGFAFSSEL
PALVRLKKWPSVTVSPKAVSLFFRHQYIPAPYTIYNETQKLPPAHAMLVDFNGCIQQKWRYWTPAFHPSVTYSLQDAIKD
AHNSVTESIDAHTLSDVPIGVFLSGGIDSTLVAARLAQRHSRSIPAFTIGYKESDFSELPYAQHAADKLGLKLYAQTLDE
KALKFAPEILSHYGEPFGDSSVIPTWHVCKLASEHVPVCLSGDGADELFGGYQKYQIWSESTPWRQLQLAGSAIAKRKLR
TAWWMLRRASRSFCDDKAFWLDHVQYYPPQGIRQLTHPDMHPYANAFAPAFDEPFLNIDKLGRFEYAQRMDLLSYLPDCL
LAKVDLASMAHGLEVRPALLDKYVFQLAATLPTDVKLHPQKGGKVVLKELLLRQGFSEEFVYRPKQGFALPLRHWFISGQ
PANNMLCELVSSYKRELSEHLTVSDIERMLTAHSPACDYSGPLWLAFAFTCWVANTSK

Sequences:

>Translated_618_residues
MCGIFGIISQLVPPSKRELETSCELLAHRGPDGYGYFIKGPVGFAHRRLSIIDIAQGSQPMSTQEGELTITYNGEIYNFR
HIKASLVAKGYSFQTNSDTEVILAAYKEWGTECVQHLRGMFAFAIADHIRQTVFLARDPFGIKPLVYRESSLGFAFSSEL
PALVRLKKWPSVTVSPKAVSLFFRHQYIPAPYTIYNETQKLPPAHAMLVDFNGCIQQKWRYWTPAFHPSVTYSLQDAIKD
AHNSVTESIDAHTLSDVPIGVFLSGGIDSTLVAARLAQRHSRSIPAFTIGYKESDFSELPYAQHAADKLGLKLYAQTLDE
KALKFAPEILSHYGEPFGDSSVIPTWHVCKLASEHVPVCLSGDGADELFGGYQKYQIWSESTPWRQLQLAGSAIAKRKLR
TAWWMLRRASRSFCDDKAFWLDHVQYYPPQGIRQLTHPDMHPYANAFAPAFDEPFLNIDKLGRFEYAQRMDLLSYLPDCL
LAKVDLASMAHGLEVRPALLDKYVFQLAATLPTDVKLHPQKGGKVVLKELLLRQGFSEEFVYRPKQGFALPLRHWFISGQ
PANNMLCELVSSYKRELSEHLTVSDIERMLTAHSPACDYSGPLWLAFAFTCWVANTSK
>Mature_618_residues
MCGIFGIISQLVPPSKRELETSCELLAHRGPDGYGYFIKGPVGFAHRRLSIIDIAQGSQPMSTQEGELTITYNGEIYNFR
HIKASLVAKGYSFQTNSDTEVILAAYKEWGTECVQHLRGMFAFAIADHIRQTVFLARDPFGIKPLVYRESSLGFAFSSEL
PALVRLKKWPSVTVSPKAVSLFFRHQYIPAPYTIYNETQKLPPAHAMLVDFNGCIQQKWRYWTPAFHPSVTYSLQDAIKD
AHNSVTESIDAHTLSDVPIGVFLSGGIDSTLVAARLAQRHSRSIPAFTIGYKESDFSELPYAQHAADKLGLKLYAQTLDE
KALKFAPEILSHYGEPFGDSSVIPTWHVCKLASEHVPVCLSGDGADELFGGYQKYQIWSESTPWRQLQLAGSAIAKRKLR
TAWWMLRRASRSFCDDKAFWLDHVQYYPPQGIRQLTHPDMHPYANAFAPAFDEPFLNIDKLGRFEYAQRMDLLSYLPDCL
LAKVDLASMAHGLEVRPALLDKYVFQLAATLPTDVKLHPQKGGKVVLKELLLRQGFSEEFVYRPKQGFALPLRHWFISGQ
PANNMLCELVSSYKRELSEHLTVSDIERMLTAHSPACDYSGPLWLAFAFTCWVANTSK

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=398, Percent_Identity=27.6381909547739, Blast_Score=116, Evalue=8e-26,
Organism=Homo sapiens, GI168229252, Length=398, Percent_Identity=27.6381909547739, Blast_Score=116, Evalue=8e-26,
Organism=Homo sapiens, GI168229250, Length=398, Percent_Identity=27.6381909547739, Blast_Score=116, Evalue=8e-26,
Organism=Homo sapiens, GI296010848, Length=373, Percent_Identity=27.6139410187668, Blast_Score=113, Evalue=5e-25,
Organism=Escherichia coli, GI1786889, Length=395, Percent_Identity=31.8987341772152, Blast_Score=153, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI71993933, Length=391, Percent_Identity=28.1329923273657, Blast_Score=116, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI25147557, Length=391, Percent_Identity=28.1329923273657, Blast_Score=115, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI25147560, Length=304, Percent_Identity=28.9473684210526, Blast_Score=101, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6321563, Length=417, Percent_Identity=26.378896882494, Blast_Score=122, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6325403, Length=415, Percent_Identity=26.7469879518072, Blast_Score=119, Evalue=1e-27,
Organism=Drosophila melanogaster, GI45553209, Length=403, Percent_Identity=29.5285359801489, Blast_Score=144, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 69644; Mature: 69644

Theoretical pI: Translated: 7.80; Mature: 7.80

Prosite motif: PS00443 GATASE_TYPE_II ; PS00104 EPSP_SYNTHASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIFGIISQLVPPSKRELETSCELLAHRGPDGYGYFIKGPVGFAHRRLSIIDIAQGSQP
CCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHCEEEEEEECCCCCC
MSTQEGELTITYNGEIYNFRHIKASLVAKGYSFQTNSDTEVILAAYKEWGTECVQHLRGM
CCCCCCEEEEEECCEEEEEHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
FAFAIADHIRQTVFLARDPFGIKPLVYRESSLGFAFSSELPALVRLKKWPSVTVSPKAVS
HHHHHHHHHHHHHHEECCCCCCCCEEEECCCCCEEECCCCCHHHHHHCCCCCEECHHHHH
LFFRHQYIPAPYTIYNETQKLPPAHAMLVDFNGCIQQKWRYWTPAFHPSVTYSLQDAIKD
HHHHHHCCCCCEEEECCCCCCCCCCEEEEECCHHHHHHHHCCCCCCCCCEEHHHHHHHHH
AHNSVTESIDAHTLSDVPIGVFLSGGIDSTLVAARLAQRHSRSIPAFTIGYKESDFSELP
HHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCC
YAQHAADKLGLKLYAQTLDEKALKFAPEILSHYGEPFGDSSVIPTWHVCKLASEHVPVCL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCEEE
SGDGADELFGGYQKYQIWSESTPWRQLQLAGSAIAKRKLRTAWWMLRRASRSFCDDKAFW
CCCCHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
LDHVQYYPPQGIRQLTHPDMHPYANAFAPAFDEPFLNIDKLGRFEYAQRMDLLSYLPDCL
HHHHHCCCCHHHHHHCCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHH
LAKVDLASMAHGLEVRPALLDKYVFQLAATLPTDVKLHPQKGGKVVLKELLLRQGFSEEF
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHCCCCHHH
VYRPKQGFALPLRHWFISGQPANNMLCELVSSYKRELSEHLTVSDIERMLTAHSPACDYS
CCCCCCCCCCCHHHHEECCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCC
GPLWLAFAFTCWVANTSK
CCHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MCGIFGIISQLVPPSKRELETSCELLAHRGPDGYGYFIKGPVGFAHRRLSIIDIAQGSQP
CCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHCEEEEEEECCCCCC
MSTQEGELTITYNGEIYNFRHIKASLVAKGYSFQTNSDTEVILAAYKEWGTECVQHLRGM
CCCCCCEEEEEECCEEEEEHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
FAFAIADHIRQTVFLARDPFGIKPLVYRESSLGFAFSSELPALVRLKKWPSVTVSPKAVS
HHHHHHHHHHHHHHEECCCCCCCCEEEECCCCCEEECCCCCHHHHHHCCCCCEECHHHHH
LFFRHQYIPAPYTIYNETQKLPPAHAMLVDFNGCIQQKWRYWTPAFHPSVTYSLQDAIKD
HHHHHHCCCCCEEEECCCCCCCCCCEEEEECCHHHHHHHHCCCCCCCCCEEHHHHHHHHH
AHNSVTESIDAHTLSDVPIGVFLSGGIDSTLVAARLAQRHSRSIPAFTIGYKESDFSELP
HHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCC
YAQHAADKLGLKLYAQTLDEKALKFAPEILSHYGEPFGDSSVIPTWHVCKLASEHVPVCL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCEEE
SGDGADELFGGYQKYQIWSESTPWRQLQLAGSAIAKRKLRTAWWMLRRASRSFCDDKAFW
CCCCHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
LDHVQYYPPQGIRQLTHPDMHPYANAFAPAFDEPFLNIDKLGRFEYAQRMDLLSYLPDCL
HHHHHCCCCHHHHHHCCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHH
LAKVDLASMAHGLEVRPALLDKYVFQLAATLPTDVKLHPQKGGKVVLKELLLRQGFSEEF
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHCCCCHHH
VYRPKQGFALPLRHWFISGQPANNMLCELVSSYKRELSEHLTVSDIERMLTAHSPACDYS
CCCCCCCCCCCHHHHEECCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCC
GPLWLAFAFTCWVANTSK
CCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]