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The map label for this gene is hsdS [H]

Identifier: 78357539

GI number: 78357539

Start: 2518688

End: 2520385

Strand: Reverse

Name: hsdS [H]

Synonym: Dde_2496

Alternate gene names: 78357539

Gene position: 2520385-2518688 (Counterclockwise)

Preceding gene: 78357540

Following gene: 78357538

Centisome position: 67.57

GC content: 49.35

Gene sequence:

>1698_bases
ATGAATAAGCAAACCGCCCATCTGCTGGAACAGCACTTCGACACCGCCTTTGCCACGCCCGATGGCGTCACCAAGCTGCG
CGAATTGATCCTGACCCTCGCCATGCAGGGTAAGCTGGTGGCGCAGGACCCGAATGATCCCCCTGCTTCGGAGTTGTTGA
AGGAGATCGAGGCCGACAAAAAACGCCAAGTCAAAGCAGGTAAAATCAAGAAACCAAAATCTCTTCCTGCTATTGATCAA
TCGGAAGCCGCCTATGTTTTACCGCAAAGCTGGACATGGACGCGCCTCGGCACCATCGGCAATATTTTTAACGGCAACAG
CATCAATGCCAGAGAGAAGGAAACCAAATACGCTGGAGCCAATGGTTTGACCTACATTGCAACCAAGGATGTTGGCTATG
GGCTTGACGCGCTGGATTATAAAAACGGGATATATATTCCTGAGTCCGAGGACAAATTCAAAATTGCCCACCAGGGTGCG
GTGCTGATCTGCGCTGAAGGCGGCAGCGCTGGCAAAAAATGCGGCATCACAGAGCAGGATATCTGTTTTGGCAACAAGCT
GTTTGCTAATGAACTTTTTGGTGGGATTCCCTCGAAATTCATCCTCTATCTGTATCTCTCTCCCGTATTCAGAGAATCCT
TCAACGCAGCCATGACTGGCATCATCGGCGGCGTATCTATTGCCAAATTTCTGGAGCTGCCGGTTCCTCTTCCTCCGTTA
AAGGAACAACACCGCATCGTCGACAAAATCGATCAGTTGATGGCCCGTTGCGATGAGCTGGAAAATCTGCGCACGGAGCG
GGAGGAGAAGCGGCTGGCTGTCCACGCCGCCGCCATCAAACAACTGCTCGACGCGCCGGACGGCTCAGCCTGGGACTTTA
TTGAGCAGCACTTCGGCGAACTCTACACCGTCAAGGAAAACGTCACCGAACTGCGCAAGGGCATCCTTCAGCTCGCCGTC
ATGGGGCGCCTTTCTGAGCAAAAAACGAATGATGAATCAGTTTCTACCCTTCTGACTAATGTCCACGCTGAGAGACAAAG
GCTGAAAATAAGGAAAACAACGGATTTAATAAATTCACCAAGGCCTCTTGGTTATGAAATACCCGAACAGTGGAAATGGG
TATGCCTTGATGACGTTTTAATTTATGGACCTACAAATGGTTTTTCACCAAGGGCTGTTGACTACGAAACCAATATCCGC
TCCTTAACTCTAAGCGCAACTACATCAGGTACATTCAAAGGAGAGTATTCAAAATTCATTGACGCTGATATTTCAAATGA
CTCCGATCTCTGGCTACGTGACGGCGATATTTTGGTGCAGCGTGGAAATACAATCGAGTACGTCGGAGTATCCGCCGTTT
ACCGTGGCAATCCCGGCGTCTATGTCTATCCCGACTTGATGATGAAACTTCGTGTCTCTAGCCATATGGACACGGATTAT
GTTTATTACGCAATGAGTTCAGTTCCGGCCAGGGAGTATTTGCGGGCACATGCATCCGGTACATCTGGAACAATGCCCAA
AATTAACCAAAAGACGCTAAAAAGCCTCCCCATCCCAGTGCCTCCTTTAGAAGAACAGCACCGCATTGTTGTCAAGATAA
AGAGACTAATGGATTTGTGTGAAATTCTTGACCAGCAGATTGACGACGCCACTGGTAAACAGACCGAACTGCTCAACGCC
GTGATGGCGCAGGCATAG

Upstream 100 bases:

>100_bases
GAGTTTGAGAAATACCGTATCGTTCAGGACCGGCTGTTTGAGAGCGATTTCGACCGGATGCTGAAACAGATCGAAGCCGT
TCAGAAACCGGGAGGCGATG

Downstream 100 bases:

>100_bases
GGAGATGCCATGCGCCTGACATCGCTCTATATCGGCCAGTACAAGAACCTTCGGGATTTTTCCCTGAGCTTCGACGGCGA
CAGCTTTATCGATGTTTTTG

Product: subunit S of type I restriction-modification system

Products: NA

Alternate protein names: S.EcoAI; Type I restriction enzyme EcoAI specificity protein; S protein [H]

Number of amino acids: Translated: 565; Mature: 565

Protein sequence:

>565_residues
MNKQTAHLLEQHFDTAFATPDGVTKLRELILTLAMQGKLVAQDPNDPPASELLKEIEADKKRQVKAGKIKKPKSLPAIDQ
SEAAYVLPQSWTWTRLGTIGNIFNGNSINAREKETKYAGANGLTYIATKDVGYGLDALDYKNGIYIPESEDKFKIAHQGA
VLICAEGGSAGKKCGITEQDICFGNKLFANELFGGIPSKFILYLYLSPVFRESFNAAMTGIIGGVSIAKFLELPVPLPPL
KEQHRIVDKIDQLMARCDELENLRTEREEKRLAVHAAAIKQLLDAPDGSAWDFIEQHFGELYTVKENVTELRKGILQLAV
MGRLSEQKTNDESVSTLLTNVHAERQRLKIRKTTDLINSPRPLGYEIPEQWKWVCLDDVLIYGPTNGFSPRAVDYETNIR
SLTLSATTSGTFKGEYSKFIDADISNDSDLWLRDGDILVQRGNTIEYVGVSAVYRGNPGVYVYPDLMMKLRVSSHMDTDY
VYYAMSSVPAREYLRAHASGTSGTMPKINQKTLKSLPIPVPPLEEQHRIVVKIKRLMDLCEILDQQIDDATGKQTELLNA
VMAQA

Sequences:

>Translated_565_residues
MNKQTAHLLEQHFDTAFATPDGVTKLRELILTLAMQGKLVAQDPNDPPASELLKEIEADKKRQVKAGKIKKPKSLPAIDQ
SEAAYVLPQSWTWTRLGTIGNIFNGNSINAREKETKYAGANGLTYIATKDVGYGLDALDYKNGIYIPESEDKFKIAHQGA
VLICAEGGSAGKKCGITEQDICFGNKLFANELFGGIPSKFILYLYLSPVFRESFNAAMTGIIGGVSIAKFLELPVPLPPL
KEQHRIVDKIDQLMARCDELENLRTEREEKRLAVHAAAIKQLLDAPDGSAWDFIEQHFGELYTVKENVTELRKGILQLAV
MGRLSEQKTNDESVSTLLTNVHAERQRLKIRKTTDLINSPRPLGYEIPEQWKWVCLDDVLIYGPTNGFSPRAVDYETNIR
SLTLSATTSGTFKGEYSKFIDADISNDSDLWLRDGDILVQRGNTIEYVGVSAVYRGNPGVYVYPDLMMKLRVSSHMDTDY
VYYAMSSVPAREYLRAHASGTSGTMPKINQKTLKSLPIPVPPLEEQHRIVVKIKRLMDLCEILDQQIDDATGKQTELLNA
VMAQA
>Mature_565_residues
MNKQTAHLLEQHFDTAFATPDGVTKLRELILTLAMQGKLVAQDPNDPPASELLKEIEADKKRQVKAGKIKKPKSLPAIDQ
SEAAYVLPQSWTWTRLGTIGNIFNGNSINAREKETKYAGANGLTYIATKDVGYGLDALDYKNGIYIPESEDKFKIAHQGA
VLICAEGGSAGKKCGITEQDICFGNKLFANELFGGIPSKFILYLYLSPVFRESFNAAMTGIIGGVSIAKFLELPVPLPPL
KEQHRIVDKIDQLMARCDELENLRTEREEKRLAVHAAAIKQLLDAPDGSAWDFIEQHFGELYTVKENVTELRKGILQLAV
MGRLSEQKTNDESVSTLLTNVHAERQRLKIRKTTDLINSPRPLGYEIPEQWKWVCLDDVLIYGPTNGFSPRAVDYETNIR
SLTLSATTSGTFKGEYSKFIDADISNDSDLWLRDGDILVQRGNTIEYVGVSAVYRGNPGVYVYPDLMMKLRVSSHMDTDY
VYYAMSSVPAREYLRAHASGTSGTMPKINQKTLKSLPIPVPPLEEQHRIVVKIKRLMDLCEILDQQIDDATGKQTELLNA
VMAQA

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0732

COG function: function code V; Restriction endonuclease S subunits

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type-I restriction system S methylase family [H]

Homologues:

Organism=Escherichia coli, GI1790807, Length=497, Percent_Identity=21.9315895372233, Blast_Score=89, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000055 [H]

Pfam domain/function: PF01420 Methylase_S [H]

EC number: NA

Molecular weight: Translated: 62974; Mature: 62974

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKQTAHLLEQHFDTAFATPDGVTKLRELILTLAMQGKLVAQDPNDPPASELLKEIEADK
CCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHH
KRQVKAGKIKKPKSLPAIDQSEAAYVLPQSWTWTRLGTIGNIFNGNSINAREKETKYAGA
HHCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCHHHCCCCCCCCCCCCCCCHHHHHHCCC
NGLTYIATKDVGYGLDALDYKNGIYIPESEDKFKIAHQGAVLICAEGGSAGKKCGITEQD
CCEEEEEECCCCCCCCCEECCCCEECCCCCCCEEEEECCEEEEEECCCCCCCCCCCCCHH
ICFGNKLFANELFGGIPSKFILYLYLSPVFRESFNAAMTGIIGGVSIAKFLELPVPLPPL
HHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
KEQHRIVDKIDQLMARCDELENLRTEREEKRLAVHAAAIKQLLDAPDGSAWDFIEQHFGE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCC
LYTVKENVTELRKGILQLAVMGRLSEQKTNDESVSTLLTNVHAERQRLKIRKTTDLINSP
EEEHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RPLGYEIPEQWKWVCLDDVLIYGPTNGFSPRAVDYETNIRSLTLSATTSGTFKGEYSKFI
CCCCCCCCCCCCEEEEEEEEEECCCCCCCCCEEEECCCCEEEEEEECCCCCCCCCHHHHH
DADISNDSDLWLRDGDILVQRGNTIEYVGVSAVYRGNPGVYVYPDLMMKLRVSSHMDTDY
CCCCCCCCCEEEECCCEEEECCCEEEEEEEEEEEECCCCEEECHHHHHHHHHHHCCCCCE
VYYAMSSVPAREYLRAHASGTSGTMPKINQKTLKSLPIPVPPLEEQHRIVVKIKRLMDLC
EEEEHHCCCHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHH
EILDQQIDDATGKQTELLNAVMAQA
HHHHHHHCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MNKQTAHLLEQHFDTAFATPDGVTKLRELILTLAMQGKLVAQDPNDPPASELLKEIEADK
CCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHH
KRQVKAGKIKKPKSLPAIDQSEAAYVLPQSWTWTRLGTIGNIFNGNSINAREKETKYAGA
HHCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCHHHCCCCCCCCCCCCCCCHHHHHHCCC
NGLTYIATKDVGYGLDALDYKNGIYIPESEDKFKIAHQGAVLICAEGGSAGKKCGITEQD
CCEEEEEECCCCCCCCCEECCCCEECCCCCCCEEEEECCEEEEEECCCCCCCCCCCCCHH
ICFGNKLFANELFGGIPSKFILYLYLSPVFRESFNAAMTGIIGGVSIAKFLELPVPLPPL
HHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
KEQHRIVDKIDQLMARCDELENLRTEREEKRLAVHAAAIKQLLDAPDGSAWDFIEQHFGE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCC
LYTVKENVTELRKGILQLAVMGRLSEQKTNDESVSTLLTNVHAERQRLKIRKTTDLINSP
EEEHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RPLGYEIPEQWKWVCLDDVLIYGPTNGFSPRAVDYETNIRSLTLSATTSGTFKGEYSKFI
CCCCCCCCCCCCEEEEEEEEEECCCCCCCCCEEEECCCCEEEEEEECCCCCCCCCHHHHH
DADISNDSDLWLRDGDILVQRGNTIEYVGVSAVYRGNPGVYVYPDLMMKLRVSSHMDTDY
CCCCCCCCCEEEECCCEEEECCCEEEEEEEEEEEECCCCEEECHHHHHHHHHHHCCCCCE
VYYAMSSVPAREYLRAHASGTSGTMPKINQKTLKSLPIPVPPLEEQHRIVVKIKRLMDLC
EEEEHHCCCHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHH
EILDQQIDDATGKQTELLNAVMAQA
HHHHHHHCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2642743 [H]