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The map label for this gene is dmlR [H]

Identifier: 78357533

GI number: 78357533

Start: 2509640

End: 2510533

Strand: Reverse

Name: dmlR [H]

Synonym: Dde_2490

Alternate gene names: 78357533

Gene position: 2510533-2509640 (Counterclockwise)

Preceding gene: 78357536

Following gene: 78357531

Centisome position: 67.3

GC content: 55.82

Gene sequence:

>894_bases
GTGGGCATAATGGTGCAGGACTTTTTGAACGACGTGCCGTTGCTGGTGGAAGTGGCCCGGCAGAAAAGTTTTTCCAAGGC
AGCTGAGGCGCTGGGCATAGGTGTTTCCACGCTTTCAAGGCGTATCAAATTGCTGGAAAAGCGTATGGGGGTTCTGCTTT
TTTACCGCGATACGCGTAATGTCGAATTGACGGACAACGGTGCGTACCTGCTGGACCGCTGCGGTTTTGTGCTGGAAGAA
GTGCAGAAGGCGTATGATTCTGTGGTGCTGAATATGCAAAAGCCTTCCGGACTGGTGCGGGTATGCATGTTTCTTGATCT
TTACGACGGACTGCTTAAAAAAGTGCTTCTTGATTTTGCAGCCAGATGGCCGGACATCAGTCTTGAGCTGACGTTTCAGG
ACCATCCCGTGGATATGCGCACCGCCCCCTTTGATGTGGCTTTTGTTACCGGAAACGCCATTGCGCCTGCTCTGGTCACA
CGCAAGCTGCTGACCATAGAACCTTTTCTGTATGCCTCGCCTTTATTGCTTGAACGCTATCCGATGCCCCGCGAACCCGA
CGACCTGCATCGCATGCCCTGCATTGTGCTGCAGCGTTTCGGCGGGCGGTGGCCCATGCACAATGGCAGCCGGCAGGTGA
CTGTGGATGTTGTGCCCCGATACAGTTTCAGCTCTGTAGAGATGTGCCGCGATTTTCTGCTGGCCGGTCACGGAGTGGCC
CTGCTGCGTAAAGAGCGCGCCCGGCCGGATGAAAAGGCGGGGCGGCTTGTACGCCTGCTTCCCGACTGGAGCGGCGGGTT
TGTTCATGATGTGAATCTGGTGATGGGATCAAGACAGCTCCCCATGCGGGTGCGCCTGTTTGTCGACCACGTACTGGCGC
AGTTTGCGCCATAG

Upstream 100 bases:

>100_bases
TGTGAAAGTTGGCTCCGGAGATGATTGCTTTTCAGTATGCCCTTTCATACCATGCTCATGGATAAAATAAACAACGCTTG
CAGAAAACTCTTTTCCGATG

Downstream 100 bases:

>100_bases
CCGCTGACAACCGGCAGATATGTCTGCCGGATTTGCAGCTGGTGTGACTGTCTTCCATAAAAATGCGGGGCAAAATGGCA
ATGTTGCTGTTTTACTCCGT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MGIMVQDFLNDVPLLVEVARQKSFSKAAEALGIGVSTLSRRIKLLEKRMGVLLFYRDTRNVELTDNGAYLLDRCGFVLEE
VQKAYDSVVLNMQKPSGLVRVCMFLDLYDGLLKKVLLDFAARWPDISLELTFQDHPVDMRTAPFDVAFVTGNAIAPALVT
RKLLTIEPFLYASPLLLERYPMPREPDDLHRMPCIVLQRFGGRWPMHNGSRQVTVDVVPRYSFSSVEMCRDFLLAGHGVA
LLRKERARPDEKAGRLVRLLPDWSGGFVHDVNLVMGSRQLPMRVRLFVDHVLAQFAP

Sequences:

>Translated_297_residues
MGIMVQDFLNDVPLLVEVARQKSFSKAAEALGIGVSTLSRRIKLLEKRMGVLLFYRDTRNVELTDNGAYLLDRCGFVLEE
VQKAYDSVVLNMQKPSGLVRVCMFLDLYDGLLKKVLLDFAARWPDISLELTFQDHPVDMRTAPFDVAFVTGNAIAPALVT
RKLLTIEPFLYASPLLLERYPMPREPDDLHRMPCIVLQRFGGRWPMHNGSRQVTVDVVPRYSFSSVEMCRDFLLAGHGVA
LLRKERARPDEKAGRLVRLLPDWSGGFVHDVNLVMGSRQLPMRVRLFVDHVLAQFAP
>Mature_296_residues
GIMVQDFLNDVPLLVEVARQKSFSKAAEALGIGVSTLSRRIKLLEKRMGVLLFYRDTRNVELTDNGAYLLDRCGFVLEEV
QKAYDSVVLNMQKPSGLVRVCMFLDLYDGLLKKVLLDFAARWPDISLELTFQDHPVDMRTAPFDVAFVTGNAIAPALVTR
KLLTIEPFLYASPLLLERYPMPREPDDLHRMPCIVLQRFGGRWPMHNGSRQVTVDVVPRYSFSSVEMCRDFLLAGHGVAL
LRKERARPDEKAGRLVRLLPDWSGGFVHDVNLVMGSRQLPMRVRLFVDHVLAQFAP

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=259, Percent_Identity=29.3436293436293, Blast_Score=117, Evalue=6e-28,
Organism=Escherichia coli, GI1787128, Length=292, Percent_Identity=28.7671232876712, Blast_Score=107, Evalue=6e-25,
Organism=Escherichia coli, GI1789440, Length=258, Percent_Identity=25.1937984496124, Blast_Score=107, Evalue=8e-25,
Organism=Escherichia coli, GI1786401, Length=290, Percent_Identity=27.5862068965517, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI145693193, Length=293, Percent_Identity=28.6689419795222, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1787589, Length=271, Percent_Identity=26.1992619926199, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1789173, Length=254, Percent_Identity=28.740157480315, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1788706, Length=167, Percent_Identity=29.940119760479, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789639, Length=181, Percent_Identity=25.414364640884, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1788748, Length=128, Percent_Identity=34.375, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33714; Mature: 33583

Theoretical pI: Translated: 8.88; Mature: 8.88

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIMVQDFLNDVPLLVEVARQKSFSKAAEALGIGVSTLSRRIKLLEKRMGVLLFYRDTRN
CCEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCEEEEEECCCC
VELTDNGAYLLDRCGFVLEEVQKAYDSVVLNMQKPSGLVRVCMFLDLYDGLLKKVLLDFA
EEEECCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ARWPDISLELTFQDHPVDMRTAPFDVAFVTGNAIAPALVTRKLLTIEPFLYASPLLLERY
HCCCCCEEEEEECCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHCCHHHHHCCHHHHCC
PMPREPDDLHRMPCIVLQRFGGRWPMHNGSRQVTVDVVPRYSFSSVEMCRDFLLAGHGVA
CCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCHH
LLRKERARPDEKAGRLVRLLPDWSGGFVHDVNLVMGSRQLPMRVRLFVDHVLAQFAP
HHHHHHCCCCHHHCCEEEECCCCCCCEEEEEHEEECCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GIMVQDFLNDVPLLVEVARQKSFSKAAEALGIGVSTLSRRIKLLEKRMGVLLFYRDTRN
CEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCEEEEEECCCC
VELTDNGAYLLDRCGFVLEEVQKAYDSVVLNMQKPSGLVRVCMFLDLYDGLLKKVLLDFA
EEEECCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ARWPDISLELTFQDHPVDMRTAPFDVAFVTGNAIAPALVTRKLLTIEPFLYASPLLLERY
HCCCCCEEEEEECCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHCCHHHHHCCHHHHCC
PMPREPDDLHRMPCIVLQRFGGRWPMHNGSRQVTVDVVPRYSFSSVEMCRDFLLAGHGVA
CCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCHH
LLRKERARPDEKAGRLVRLLPDWSGGFVHDVNLVMGSRQLPMRVRLFVDHVLAQFAP
HHHHHHCCCCHHHCCEEEECCCCCCCEEEEEHEEECCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]