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The map label for this gene is mleN [H]

Identifier: 78357519

GI number: 78357519

Start: 2495094

End: 2496419

Strand: Reverse

Name: mleN [H]

Synonym: Dde_2476

Alternate gene names: 78357519

Gene position: 2496419-2495094 (Counterclockwise)

Preceding gene: 78357520

Following gene: 78357517

Centisome position: 66.92

GC content: 58.14

Gene sequence:

>1326_bases
TTGAACAGTAAACCGTCTTTTTCGGCCCTGCTGCCGCTTGTGTTTTTTCTTGCGCTGTTTATCGGCACCGGTGCCGTGCT
GACGGCCAACGGCACGAAAATGGCTTTTTACCAGCTGTCCGCCGCCGTAGCCATACTGCCTGCCATCGCCTTCAGCCTGC
TGCTGGGCAAACTGCGCGGCGGCCAGCGTCTGGACAAGCAGCTCGGGGTTTTCATCAGCGGCGTGGGTGACGGCAACATC
ATAACCATGTGCCTCATCTATCTGCTTGCCGGTGCCTTTGCTTCGGTGGCTACGGCCATCGGCGGCGTCAGTTCCACGGT
CAATCTCGGGCTTGATCTTGTGCCGCCGGCCTTTGTGCTGCCCGGCCTGTTTCTTATCGGGGCTTTTGTCTCCACTGCCA
TGGGCACATCCATGGGCACCATAGCCGCCATTGCCCCTATTGCCGTGGGCGTGGGCGAACAGACAACCATCGGCCTGCCG
CTGCTTATGGGCGCTGTGCTGGGCGGCGCCATGTTCGGCGACAACCTTTCCATGATTTCTGATACCACCATCGCCGCCAC
GCGTACGCAGGGCTGCGAGATGAAAGATAAATTCCGTGTAAACCTGTACATAGCCCTGCCCGCGGCCGTGCTGGCAGTGG
CCGGATTCGCCCTGCTGGGTGCCGACGGCGCGGTGACTGTTTCCGGTGATTACAGCGTTATCAAAGCGCTTCCCTATATG
GTTATTCTGGGTATGGCCGTGGCCGGGGTAAACGTCTTTGTGGTGCTGGCGTGCGGCATCGTGTTTACGGGTGCTGTGGG
GCTGCTGACAATGCCGGAATATTCGCTGATAGGATTTTCGCAGGACATTTTTAACGGCTTCAAGGGAATGCAGGAAATCA
TGGTTCTTTCCATGCTGATCGGCGGTCTGGGCGAACTGATCAGAGTGCAGGGCGGTATCAAGTGGCTGCTGGGCCGTATA
GATGCGCTTACTGCACGCTCGCGTAGCGACAATCAGGCCCGTACCGGCGAGTTGAGCATTGCGGGGCTTGTCTCGGTGGC
GGATGCCTGCACGGCAAACAACACCGTGGCCATCATTCTTTCCGGCGGTCTGGCAAAAGAGATTGCCGACAAGAACGGCA
TAGACCCCCGCCGCAGCGCAAGCATACTTGATATATTCTCCTGTGTTTTTCAGGGAATCATGCCGTACGCGGCGCAGGTG
CTGCTTGCCGGTTCCATAGCGGGCATATCGCCGCTGGAAGTTGTCGGCGGTAACTACTACTGCTTTATTCTTGCCGTTGT
GGGGCTGCTGGCCATCATGACGGGCAGGCCCCGGATTAAATCTTAA

Upstream 100 bases:

>100_bases
GCGTGTGTCATCATCGTGCCAGCGCTCCGGTTGCGCCGACCGGCGAGCAGCAGTGAAGGGGCTGCCGCGCTGCCATTCCT
GAACGGGGGAGGATACCACC

Downstream 100 bases:

>100_bases
TATCAGGAATTGTTCCTGAATATGTATGTGCCACCCCCCGCCGGGGGTGACATTTTTTTATGGAAAATATAAAAAAATAA
TTTTGAAAAACAGAGTGTTA

Product: Na+/H+ antiporter family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 441; Mature: 441

Protein sequence:

>441_residues
MNSKPSFSALLPLVFFLALFIGTGAVLTANGTKMAFYQLSAAVAILPAIAFSLLLGKLRGGQRLDKQLGVFISGVGDGNI
ITMCLIYLLAGAFASVATAIGGVSSTVNLGLDLVPPAFVLPGLFLIGAFVSTAMGTSMGTIAAIAPIAVGVGEQTTIGLP
LLMGAVLGGAMFGDNLSMISDTTIAATRTQGCEMKDKFRVNLYIALPAAVLAVAGFALLGADGAVTVSGDYSVIKALPYM
VILGMAVAGVNVFVVLACGIVFTGAVGLLTMPEYSLIGFSQDIFNGFKGMQEIMVLSMLIGGLGELIRVQGGIKWLLGRI
DALTARSRSDNQARTGELSIAGLVSVADACTANNTVAIILSGGLAKEIADKNGIDPRRSASILDIFSCVFQGIMPYAAQV
LLAGSIAGISPLEVVGGNYYCFILAVVGLLAIMTGRPRIKS

Sequences:

>Translated_441_residues
MNSKPSFSALLPLVFFLALFIGTGAVLTANGTKMAFYQLSAAVAILPAIAFSLLLGKLRGGQRLDKQLGVFISGVGDGNI
ITMCLIYLLAGAFASVATAIGGVSSTVNLGLDLVPPAFVLPGLFLIGAFVSTAMGTSMGTIAAIAPIAVGVGEQTTIGLP
LLMGAVLGGAMFGDNLSMISDTTIAATRTQGCEMKDKFRVNLYIALPAAVLAVAGFALLGADGAVTVSGDYSVIKALPYM
VILGMAVAGVNVFVVLACGIVFTGAVGLLTMPEYSLIGFSQDIFNGFKGMQEIMVLSMLIGGLGELIRVQGGIKWLLGRI
DALTARSRSDNQARTGELSIAGLVSVADACTANNTVAIILSGGLAKEIADKNGIDPRRSASILDIFSCVFQGIMPYAAQV
LLAGSIAGISPLEVVGGNYYCFILAVVGLLAIMTGRPRIKS
>Mature_441_residues
MNSKPSFSALLPLVFFLALFIGTGAVLTANGTKMAFYQLSAAVAILPAIAFSLLLGKLRGGQRLDKQLGVFISGVGDGNI
ITMCLIYLLAGAFASVATAIGGVSSTVNLGLDLVPPAFVLPGLFLIGAFVSTAMGTSMGTIAAIAPIAVGVGEQTTIGLP
LLMGAVLGGAMFGDNLSMISDTTIAATRTQGCEMKDKFRVNLYIALPAAVLAVAGFALLGADGAVTVSGDYSVIKALPYM
VILGMAVAGVNVFVVLACGIVFTGAVGLLTMPEYSLIGFSQDIFNGFKGMQEIMVLSMLIGGLGELIRVQGGIKWLLGRI
DALTARSRSDNQARTGELSIAGLVSVADACTANNTVAIILSGGLAKEIADKNGIDPRRSASILDIFSCVFQGIMPYAAQV
LLAGSIAGISPLEVVGGNYYCFILAVVGLLAIMTGRPRIKS

Specific function: Couples proton uptake and Na(+) efflux to the substrate- product malate/lactate antiport, in an electroneutral malate- 2H(+)/Na(+)-lactate exchange. Plays a role in supporting growth to high density on malate at reduced protonmotive force [H]

COG id: COG1757

COG function: function code C; Na+/H+ antiporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nhaC Na(+)/H(+) antiporter family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004770
- InterPro:   IPR018461 [H]

Pfam domain/function: PF03553 Na_H_antiporter [H]

EC number: NA

Molecular weight: Translated: 45193; Mature: 45193

Theoretical pI: Translated: 8.08; Mature: 8.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSKPSFSALLPLVFFLALFIGTGAVLTANGTKMAFYQLSAAVAILPAIAFSLLLGKLRG
CCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCEEHHHHHHHHHHHHHHHHHHHHHHHHCC
GQRLDKQLGVFISGVGDGNIITMCLIYLLAGAFASVATAIGGVSSTVNLGLDLVPPAFVL
CHHHHHHHCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCHHHHH
PGLFLIGAFVSTAMGTSMGTIAAIAPIAVGVGEQTTIGLPLLMGAVLGGAMFGDNLSMIS
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHC
DTTIAATRTQGCEMKDKFRVNLYIALPAAVLAVAGFALLGADGAVTVSGDYSVIKALPYM
CCEEEEECCCCCCCCCCEEEEEEEECHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHH
VILGMAVAGVNVFVVLACGIVFTGAVGLLTMPEYSLIGFSQDIFNGFKGMQEIMVLSMLI
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHH
GGLGELIRVQGGIKWLLGRIDALTARSRSDNQARTGELSIAGLVSVADACTANNTVAIIL
HHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEE
SGGLAKEIADKNGIDPRRSASILDIFSCVFQGIMPYAAQVLLAGSIAGISPLEVVGGNYY
ECCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCHH
CFILAVVGLLAIMTGRPRIKS
HHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MNSKPSFSALLPLVFFLALFIGTGAVLTANGTKMAFYQLSAAVAILPAIAFSLLLGKLRG
CCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCEEHHHHHHHHHHHHHHHHHHHHHHHHCC
GQRLDKQLGVFISGVGDGNIITMCLIYLLAGAFASVATAIGGVSSTVNLGLDLVPPAFVL
CHHHHHHHCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCHHHHH
PGLFLIGAFVSTAMGTSMGTIAAIAPIAVGVGEQTTIGLPLLMGAVLGGAMFGDNLSMIS
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHC
DTTIAATRTQGCEMKDKFRVNLYIALPAAVLAVAGFALLGADGAVTVSGDYSVIKALPYM
CCEEEEECCCCCCCCCCEEEEEEEECHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHH
VILGMAVAGVNVFVVLACGIVFTGAVGLLTMPEYSLIGFSQDIFNGFKGMQEIMVLSMLI
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHH
GGLGELIRVQGGIKWLLGRIDALTARSRSDNQARTGELSIAGLVSVADACTANNTVAIIL
HHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEE
SGGLAKEIADKNGIDPRRSASILDIFSCVFQGIMPYAAQVLLAGSIAGISPLEVVGGNYY
ECCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCHH
CFILAVVGLLAIMTGRPRIKS
HHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 10903309 [H]