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The map label for this gene is miaB

Identifier: 78357421

GI number: 78357421

Start: 2401832

End: 2403226

Strand: Direct

Name: miaB

Synonym: Dde_2378

Alternate gene names: 78357421

Gene position: 2401832-2403226 (Clockwise)

Preceding gene: 78357420

Following gene: 78357422

Centisome position: 64.39

GC content: 57.56

Gene sequence:

>1395_bases
ATGCATGAAAGAACTTTCCATATAATGACCTTTGGCTGCCAGATGAACGTCAACGATTCCGACTGGCTGGCGCGTGCCCT
GGAAGCCCGCGGCTTTACACAGGTTCCGGAACATGAGGCCGCCATATACATCATCAACACCTGTTCAGTCCGCGACAAAC
CGGAACAGAAAGTCTATTCACTGCTCGGACGCATACGCAGAGAAACAAAAAACAGACGGAATGTGACCGTATGTGTGGGC
GGCTGCGTGGCGCAGCAGATAGGCAAAGGCTTTTTCAAGCGGTTCAGTCAGGTCCGGCTGGTATTCGGCACCGACGGGGC
AGCCAGCGCGCCGCAGGCCATCGAACGGCTGGTGCAGGAACCGCACGCCCGCATCAGCCTGCTGGATTTTTCCGAAGAAT
TTCCCGAACGCGATGCAGGGTGGGAAAACGGCGAAGTACCTGTCTCCGCGTATGTCAATATTATGCAGGGGTGCAACAAC
TTCTGCGCATACTGCATTGTGCCCTACACCCGCGGCAGGCAGAAATCGCGCAGTTCGGCCGCCGTGCTGGACGAATGCAG
AACGCTGGTCGGCAACGGAGCGCGGGAAATCACCCTGCTGGGGCAGAACGTTAATTCCTACGGGCTTGATCCGCACGGCG
ACGGGACAACGTTTGCCCGGCTGCTGCATGACGTGGCTGCCATACCCGGTCTTGAAAGGCTGCGTTTCATGACTCCGCAT
CCCAAAGACATAGCCGGTGAAGTCATTGAAGCCTTCGGAGCGCTGAAAAATCTCTGCCCCAGAGTACACCTGCCGCTGCA
GTCCGGTTCTGACAGAGTGCTCAAAGCCATGGGCCGCAAATACGACATGGCCCGCTACATGGACATTGTTACCCGTCTGA
AAGCGGTCCGCCCCGACATTCAGATAACCAGCGACCTCATAGTCGGTTTTCCGGGCGAAACGGAAGCAGATTTTGAACAG
ACGCTGGAAGCCATGCGCACCGTGCCCTTTGTTCAGAGCTTTTCATTCATCTATTCCGACAGGCCCGGCACACGGGCTGA
GATGCTGCCCGGCAAGCTTTCGCGCGAAGAAAAAACCGCACGGCTTGTCAGGCTGCAGGAAGTGCAGAATGAATACAGTG
AAGCAGCGCTGCAGGCTATGGTCGGCAAAACAGTCATGGTGCTGTTTGAAAGCCCCAGCCCCAAAAGTGCAGCCGGTTCC
GGTACGGACGCACAGAATGCAGCGGAAGAATCGGGCCGGACGGCATCCTCATGGCAGGGACGGGACGAGCACGGCTTTAT
ACTGAACGTGCACCTGCCCGCACCCGCAGACCTGTACGGCAAAATAATGCCCGTCACAGTGACCGCCGCACGCAAACACT
CGCTGACGGGCGAACCCGCGGGGGAATCATGCTGA

Upstream 100 bases:

>100_bases
CGCAGCTGAGTCTGCTCCTTCCGTGTTGCCCTGTTTTCCACGGTGCGCCCGCTGTTGCCGCGCGCACTGTTTTTTATGAA
TCTATCGTACCCCGAATGCC

Downstream 100 bases:

>100_bases
TTGAAATGAAAGTTCTGGGCCTGTCGCTGGACGAGACCAGCAAAGCCCCCATACTCATCCTGCAGCAGAAGGACGGCAAG
GATGTCCTGCCCATCTGGGT

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MHERTFHIMTFGCQMNVNDSDWLARALEARGFTQVPEHEAAIYIINTCSVRDKPEQKVYSLLGRIRRETKNRRNVTVCVG
GCVAQQIGKGFFKRFSQVRLVFGTDGAASAPQAIERLVQEPHARISLLDFSEEFPERDAGWENGEVPVSAYVNIMQGCNN
FCAYCIVPYTRGRQKSRSSAAVLDECRTLVGNGAREITLLGQNVNSYGLDPHGDGTTFARLLHDVAAIPGLERLRFMTPH
PKDIAGEVIEAFGALKNLCPRVHLPLQSGSDRVLKAMGRKYDMARYMDIVTRLKAVRPDIQITSDLIVGFPGETEADFEQ
TLEAMRTVPFVQSFSFIYSDRPGTRAEMLPGKLSREEKTARLVRLQEVQNEYSEAALQAMVGKTVMVLFESPSPKSAAGS
GTDAQNAAEESGRTASSWQGRDEHGFILNVHLPAPADLYGKIMPVTVTAARKHSLTGEPAGESC

Sequences:

>Translated_464_residues
MHERTFHIMTFGCQMNVNDSDWLARALEARGFTQVPEHEAAIYIINTCSVRDKPEQKVYSLLGRIRRETKNRRNVTVCVG
GCVAQQIGKGFFKRFSQVRLVFGTDGAASAPQAIERLVQEPHARISLLDFSEEFPERDAGWENGEVPVSAYVNIMQGCNN
FCAYCIVPYTRGRQKSRSSAAVLDECRTLVGNGAREITLLGQNVNSYGLDPHGDGTTFARLLHDVAAIPGLERLRFMTPH
PKDIAGEVIEAFGALKNLCPRVHLPLQSGSDRVLKAMGRKYDMARYMDIVTRLKAVRPDIQITSDLIVGFPGETEADFEQ
TLEAMRTVPFVQSFSFIYSDRPGTRAEMLPGKLSREEKTARLVRLQEVQNEYSEAALQAMVGKTVMVLFESPSPKSAAGS
GTDAQNAAEESGRTASSWQGRDEHGFILNVHLPAPADLYGKIMPVTVTAARKHSLTGEPAGESC
>Mature_464_residues
MHERTFHIMTFGCQMNVNDSDWLARALEARGFTQVPEHEAAIYIINTCSVRDKPEQKVYSLLGRIRRETKNRRNVTVCVG
GCVAQQIGKGFFKRFSQVRLVFGTDGAASAPQAIERLVQEPHARISLLDFSEEFPERDAGWENGEVPVSAYVNIMQGCNN
FCAYCIVPYTRGRQKSRSSAAVLDECRTLVGNGAREITLLGQNVNSYGLDPHGDGTTFARLLHDVAAIPGLERLRFMTPH
PKDIAGEVIEAFGALKNLCPRVHLPLQSGSDRVLKAMGRKYDMARYMDIVTRLKAVRPDIQITSDLIVGFPGETEADFEQ
TLEAMRTVPFVQSFSFIYSDRPGTRAEMLPGKLSREEKTARLVRLQEVQNEYSEAALQAMVGKTVMVLFESPSPKSAAGS
GTDAQNAAEESGRTASSWQGRDEHGFILNVHLPAPADLYGKIMPVTVTAARKHSLTGEPAGESC

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=424, Percent_Identity=34.4339622641509, Blast_Score=247, Evalue=1e-65,
Organism=Homo sapiens, GI28872782, Length=423, Percent_Identity=34.51536643026, Blast_Score=247, Evalue=1e-65,
Organism=Homo sapiens, GI93277076, Length=472, Percent_Identity=26.4830508474576, Blast_Score=134, Evalue=2e-31,
Organism=Escherichia coli, GI1786882, Length=463, Percent_Identity=39.9568034557235, Blast_Score=303, Evalue=1e-83,
Organism=Escherichia coli, GI1787057, Length=447, Percent_Identity=27.9642058165548, Blast_Score=161, Evalue=9e-41,
Organism=Caenorhabditis elegans, GI17553146, Length=425, Percent_Identity=34.1176470588235, Blast_Score=230, Evalue=1e-60,
Organism=Caenorhabditis elegans, GI71996771, Length=316, Percent_Identity=27.5316455696203, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21356207, Length=498, Percent_Identity=31.1244979919679, Blast_Score=236, Evalue=4e-62,
Organism=Drosophila melanogaster, GI19922432, Length=465, Percent_Identity=28.3870967741935, Blast_Score=132, Evalue=5e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_DESDG (Q30YS1)

Other databases:

- EMBL:   CP000112
- RefSeq:   YP_388870.1
- ProteinModelPortal:   Q30YS1
- SMR:   Q30YS1
- STRING:   Q30YS1
- GeneID:   3757394
- GenomeReviews:   CP000112_GR
- KEGG:   dde:Dde_2378
- NMPDR:   fig|207559.3.peg.2736
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   SYHHLPQ
- ProtClustDB:   PRK14337
- BioCyc:   DDES207559:DDE_2378-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 51244; Mature: 51244

Theoretical pI: Translated: 7.27; Mature: 7.27

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHERTFHIMTFGCQMNVNDSDWLARALEARGFTQVPEHEAAIYIINTCSVRDKPEQKVYS
CCCCEEEEEEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCHHHHHHH
LLGRIRRETKNRRNVTVCVGGCVAQQIGKGFFKRFSQVRLVFGTDGAASAPQAIERLVQE
HHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHEEEEEECCCCCCCHHHHHHHHHC
PHARISLLDFSEEFPERDAGWENGEVPVSAYVNIMQGCNNFCAYCIVPYTRGRQKSRSSA
CCCEEEEEECHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHEEEEEEECCCCCCCCCHHH
AVLDECRTLVGNGAREITLLGQNVNSYGLDPHGDGTTFARLLHDVAAIPGLERLRFMTPH
HHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHCCCCC
PKDIAGEVIEAFGALKNLCPRVHLPLQSGSDRVLKAMGRKYDMARYMDIVTRLKAVRPDI
CHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHCCCC
QITSDLIVGFPGETEADFEQTLEAMRTVPFVQSFSFIYSDRPGTRAEMLPGKLSREEKTA
EEECCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCHHHHHH
RLVRLQEVQNEYSEAALQAMVGKTVMVLFESPSPKSAAGSGTDAQNAAEESGRTASSWQG
HHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCHHHHHHHCCCCCCCCCC
RDEHGFILNVHLPAPADLYGKIMPVTVTAARKHSLTGEPAGESC
CCCCCEEEEEECCCCHHHHCCEEEEEEEEHHHCCCCCCCCCCCC
>Mature Secondary Structure
MHERTFHIMTFGCQMNVNDSDWLARALEARGFTQVPEHEAAIYIINTCSVRDKPEQKVYS
CCCCEEEEEEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCHHHHHHH
LLGRIRRETKNRRNVTVCVGGCVAQQIGKGFFKRFSQVRLVFGTDGAASAPQAIERLVQE
HHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHEEEEEECCCCCCCHHHHHHHHHC
PHARISLLDFSEEFPERDAGWENGEVPVSAYVNIMQGCNNFCAYCIVPYTRGRQKSRSSA
CCCEEEEEECHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHEEEEEEECCCCCCCCCHHH
AVLDECRTLVGNGAREITLLGQNVNSYGLDPHGDGTTFARLLHDVAAIPGLERLRFMTPH
HHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHCCCCC
PKDIAGEVIEAFGALKNLCPRVHLPLQSGSDRVLKAMGRKYDMARYMDIVTRLKAVRPDI
CHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHCCCC
QITSDLIVGFPGETEADFEQTLEAMRTVPFVQSFSFIYSDRPGTRAEMLPGKLSREEKTA
EEECCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCHHHHHH
RLVRLQEVQNEYSEAALQAMVGKTVMVLFESPSPKSAAGSGTDAQNAAEESGRTASSWQG
HHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCHHHHHHHCCCCCCCCCC
RDEHGFILNVHLPAPADLYGKIMPVTVTAARKHSLTGEPAGESC
CCCCCEEEEEECCCCHHHHCCEEEEEEEEHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA