Click here to switch to the map view.

The map label for this gene is hisJ [H]

Identifier: 78357349

GI number: 78357349

Start: 2323411

End: 2324151

Strand: Direct

Name: hisJ [H]

Synonym: Dde_2306

Alternate gene names: 78357349

Gene position: 2323411-2324151 (Clockwise)

Preceding gene: 78357348

Following gene: 78357350

Centisome position: 62.29

GC content: 54.12

Gene sequence:

>741_bases
ATGCTGAAAAAAATTTGTCTGGCCATGCTCGCCGTCATGATGACAGCCAACGTGGCACTTGCGGAACGGACCATTGTTTT
TGCCCATGACGCCACCTGGCCGCCCATGGAATACATGAGCGCCGACAAAAAGGTTGTGGGCTATGCCGTTGACTACATGG
ACGCGGTAGCCAAGGAAGCCGGCTTTACGGCCGAGCACCGCAATGTGGCGTGGGACGGCATTTTTGCCGGTCTTGCCAAC
GGCGAATATGATGCCATCGCCTCTTCCGTTTCAATCACCGAACAGCGCAAACGCGCCATGGACTTCAGCATCCCCTACTT
TGAAGTCAAACAGGCAGTGGTACTGAACAAGTCCGTCGAAGCCAAATCGTTTGCCGACCTCAAGGGCAAAACCCTCGGCG
CGCAGATTGGCACCACCGGCTACTTTGCCATCAAAAAAGCAGAAGGCGTCACCCCCCGCTCTTTTGACGAAATCGGCCTT
GCCATGGAAGCGCTGTACAACGGCAACATAGACGGTGTGGTCTGCGATGACCCTGTGGCCGCCAACTACGCCCTGCAGCA
GGAAGAATACGCCAAGCGCCTGAAAATCGCCTTCATCGTTGAAGCCGAAGAAAAAGAATACTACGGCGTGGCTGTAAACA
AAGGAAACTCCGAAGTGCTGGCCCTCATCGACAAGGGCATAAAAGCCGTGAAGGAAAAAGGCATAGAAGACCAGCTGCGC
AAAAAGTGGATAGGTCAATAG

Upstream 100 bases:

>100_bases
TGCAGCCATACTCCTGCCCGTGCACATTGACAACCGGCTGCAAAATGAGCCATTATACATTTTTGTCTACTCCGGCAGAA
CACACAAAAGGAGAATGAGG

Downstream 100 bases:

>100_bases
CCGTACGGAAGTCGAATCAACGGGACTGGTGCATCCTGCACCGGTCCTTTCATTTTGCAAGCACACGGATACACTATCAT
GGCTGATCAAAAAGTCCGCA

Product: extracellular solute-binding protein

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: HBP [H]

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MLKKICLAMLAVMMTANVALAERTIVFAHDATWPPMEYMSADKKVVGYAVDYMDAVAKEAGFTAEHRNVAWDGIFAGLAN
GEYDAIASSVSITEQRKRAMDFSIPYFEVKQAVVLNKSVEAKSFADLKGKTLGAQIGTTGYFAIKKAEGVTPRSFDEIGL
AMEALYNGNIDGVVCDDPVAANYALQQEEYAKRLKIAFIVEAEEKEYYGVAVNKGNSEVLALIDKGIKAVKEKGIEDQLR
KKWIGQ

Sequences:

>Translated_246_residues
MLKKICLAMLAVMMTANVALAERTIVFAHDATWPPMEYMSADKKVVGYAVDYMDAVAKEAGFTAEHRNVAWDGIFAGLAN
GEYDAIASSVSITEQRKRAMDFSIPYFEVKQAVVLNKSVEAKSFADLKGKTLGAQIGTTGYFAIKKAEGVTPRSFDEIGL
AMEALYNGNIDGVVCDDPVAANYALQQEEYAKRLKIAFIVEAEEKEYYGVAVNKGNSEVLALIDKGIKAVKEKGIEDQLR
KKWIGQ
>Mature_246_residues
MLKKICLAMLAVMMTANVALAERTIVFAHDATWPPMEYMSADKKVVGYAVDYMDAVAKEAGFTAEHRNVAWDGIFAGLAN
GEYDAIASSVSITEQRKRAMDFSIPYFEVKQAVVLNKSVEAKSFADLKGKTLGAQIGTTGYFAIKKAEGVTPRSFDEIGL
AMEALYNGNIDGVVCDDPVAANYALQQEEYAKRLKIAFIVEAEEKEYYGVAVNKGNSEVLALIDKGIKAVKEKGIEDQLR
KKWIGQ

Specific function: Component of the high-affinity histidine permease, a binding-protein-dependent transport system. The other components are proteins hisQ, hisM, and hisP [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787085, Length=250, Percent_Identity=31.6, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1787088, Length=248, Percent_Identity=29.8387096774194, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1788228, Length=225, Percent_Identity=28.4444444444444, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI1787031, Length=245, Percent_Identity=28.9795918367347, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1788649, Length=250, Percent_Identity=24.4, Blast_Score=61, Evalue=6e-11,

Paralogues:

None

Copy number: 1180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 26970; Mature: 26970

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKKICLAMLAVMMTANVALAERTIVFAHDATWPPMEYMSADKKVVGYAVDYMDAVAKEA
CHHHHHHHHHHHHHHCCCEEHEEEEEEEECCCCCCHHHHCCCCHHHHHHHHHHHHHHHHC
GFTAEHRNVAWDGIFAGLANGEYDAIASSVSITEQRKRAMDFSIPYFEVKQAVVLNKSVE
CCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCC
AKSFADLKGKTLGAQIGTTGYFAIKKAEGVTPRSFDEIGLAMEALYNGNIDGVVCDDPVA
HHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCC
ANYALQQEEYAKRLKIAFIVEAEEKEYYGVAVNKGNSEVLALIDKGIKAVKEKGIEDQLR
CCHHHHHHHHHHHEEEEEEEEECCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCHHHHH
KKWIGQ
HHHCCC
>Mature Secondary Structure
MLKKICLAMLAVMMTANVALAERTIVFAHDATWPPMEYMSADKKVVGYAVDYMDAVAKEA
CHHHHHHHHHHHHHHCCCEEHEEEEEEEECCCCCCHHHHCCCCHHHHHHHHHHHHHHHHC
GFTAEHRNVAWDGIFAGLANGEYDAIASSVSITEQRKRAMDFSIPYFEVKQAVVLNKSVE
CCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCC
AKSFADLKGKTLGAQIGTTGYFAIKKAEGVTPRSFDEIGLAMEALYNGNIDGVVCDDPVA
HHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCC
ANYALQQEEYAKRLKIAFIVEAEEKEYYGVAVNKGNSEVLALIDKGIKAVKEKGIEDQLR
CCHHHHHHHHHHHEEEEEEEEECCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCHHHHH
KKWIGQ
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1448614 [H]