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The map label for this gene is hupD [H]

Identifier: 78357182

GI number: 78357182

Start: 2187470

End: 2187967

Strand: Direct

Name: hupD [H]

Synonym: Dde_2139

Alternate gene names: 78357182

Gene position: 2187470-2187967 (Clockwise)

Preceding gene: 78357181

Following gene: 78357183

Centisome position: 58.64

GC content: 54.82

Gene sequence:

>498_bases
ATGTGTATGGAGCAGACAAAACGCATACTTGTTCTGGGCGTCGGCAACATATTGTACACCGACGAAGGGCTGGGAGTCCG
CGCAGTGGAATCCCTCATGGACCGTTATGAGTTTTCAGACAACATTACTCTTATGGATGGCGGCACACTGGGCATCAAGC
TTATGGACGCCATCATGAGCTGCGAACAGCTGATTGTGGTTGACGCCGTACTGGGCGGCGATGCTCCGGGCTCAGTCTAC
CGGCTGACCGGCGAAGACCTGCGCAAAAGTCTCGGCTTCAATGATTCCATGCATCAGACCGACCTTGTGGATACGCTTAT
ATACTGCGAAATTGCGGGCAACCGGCCGCAGGCAGTTGTCATAGGCATGGAGCCGTATGACTACCAGTCCATGGGAACGG
AGCTTTCAGCCACTGTGGCAGAACGCCTGCCCGCAATGTGCGAGATTGTGCTCAAAGAGGTTTCCGATCATGGCGGCAGG
TGGTCCGCCAGAACATAA

Upstream 100 bases:

>100_bases
AACGAGGTGCATAAGTTCAAAGTGCTGTAATTTTGCAGCGAAACAGCAAAATGAGAGGGAGCCCGGCCTTGCGCTGGACT
CCCTTTTTTCCCTATAGTGG

Downstream 100 bases:

>100_bases
CAGAAGGGGGAAGACCGCATGTGTCTGGCAATTCCTGCGGAAGTGAACGAGCTGCTTGACGGAGACATGGCCAAGTGCCG
TGTGGGCAAAAGCGACACCT

Product: hydrogenase expression/formation protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 165; Mature: 165

Protein sequence:

>165_residues
MCMEQTKRILVLGVGNILYTDEGLGVRAVESLMDRYEFSDNITLMDGGTLGIKLMDAIMSCEQLIVVDAVLGGDAPGSVY
RLTGEDLRKSLGFNDSMHQTDLVDTLIYCEIAGNRPQAVVIGMEPYDYQSMGTELSATVAERLPAMCEIVLKEVSDHGGR
WSART

Sequences:

>Translated_165_residues
MCMEQTKRILVLGVGNILYTDEGLGVRAVESLMDRYEFSDNITLMDGGTLGIKLMDAIMSCEQLIVVDAVLGGDAPGSVY
RLTGEDLRKSLGFNDSMHQTDLVDTLIYCEIAGNRPQAVVIGMEPYDYQSMGTELSATVAERLPAMCEIVLKEVSDHGGR
WSART
>Mature_165_residues
MCMEQTKRILVLGVGNILYTDEGLGVRAVESLMDRYEFSDNITLMDGGTLGIKLMDAIMSCEQLIVVDAVLGGDAPGSVY
RLTGEDLRKSLGFNDSMHQTDLVDTLIYCEIAGNRPQAVVIGMEPYDYQSMGTELSATVAERLPAMCEIVLKEVSDHGGR
WSART

Specific function: Not known. Could be involved in the processing of hydrogenase [H]

COG id: COG0680

COG function: function code C; Ni,Fe-hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A31 family [H]

Homologues:

Organism=Escherichia coli, GI1787209, Length=140, Percent_Identity=32.8571428571429, Blast_Score=90, Evalue=9e-20,
Organism=Escherichia coli, GI1789367, Length=153, Percent_Identity=35.9477124183007, Blast_Score=84, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004419
- InterPro:   IPR000671 [H]

Pfam domain/function: PF01750 HycI [H]

EC number: 3.4.24.- [C]

Molecular weight: Translated: 18045; Mature: 18045

Theoretical pI: Translated: 4.19; Mature: 4.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
6.1 %Met     (Translated Protein)
8.5 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
6.1 %Met     (Mature Protein)
8.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCMEQTKRILVLGVGNILYTDEGLGVRAVESLMDRYEFSDNITLMDGGTLGIKLMDAIMS
CCCHHCCEEEEEECCCEEEECCCCCHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHH
CEQLIVVDAVLGGDAPGSVYRLTGEDLRKSLGFNDSMHQTDLVDTLIYCEIAGNRPQAVV
HHHHHHHHHHHCCCCCCCEEEECCHHHHHHCCCCCCCHHHHHHHHHEEEEECCCCCCEEE
IGMEPYDYQSMGTELSATVAERLPAMCEIVLKEVSDHGGRWSART
EECCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MCMEQTKRILVLGVGNILYTDEGLGVRAVESLMDRYEFSDNITLMDGGTLGIKLMDAIMS
CCCHHCCEEEEEECCCEEEECCCCCHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHH
CEQLIVVDAVLGGDAPGSVYRLTGEDLRKSLGFNDSMHQTDLVDTLIYCEIAGNRPQAVV
HHHHHHHHHHHCCCCCCCEEEECCHHHHHHCCCCCCCHHHHHHHHHEEEEECCCCCCEEE
IGMEPYDYQSMGTELSATVAERLPAMCEIVLKEVSDHGGRWSART
EECCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Ni [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1597428 [H]