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The map label for this gene is aat

Identifier: 78357141

GI number: 78357141

Start: 2147717

End: 2148463

Strand: Reverse

Name: aat

Synonym: Dde_2098

Alternate gene names: 78357141

Gene position: 2148463-2147717 (Counterclockwise)

Preceding gene: 78357142

Following gene: 78357139

Centisome position: 57.6

GC content: 60.51

Gene sequence:

>747_bases
ATGCACCTTTACGCACTGTCTGAAAAGCTGGAGTTTCCCGACCCGCAGACGGCATCGTCGGAAGGGCTGCTGGCCATAGG
GGGCGACCTCAGTGTCGCCCGGCTGGTCACTGCCTACGCAAACGGTATTTTTCCGTGGTACGATGATGACACCCCCATCC
TGTGGTGGTCGCCCGACCCGCGGTGTGTGTTGTATCCTTCGCAGCTGCATGTGCCTGCAAGCCTGCGCAGGCGTATCAAC
TCCGGGCAGTTTGAAGTGACGCTCGATACCGCTTTTGAGGCGGTTATACATGCCTGTGCCGACGCTGCGCGTCCCGGGCA
GAGCGGAACATGGATTGTAGATGAGATGATAGACGCCTATATGAATCTGCACGAGGCCGGTGTGGCGCATTCTGTGGAGG
TATGGAGCCGCGGTGCGGGTGATGGGCGCGTGCTGGCCGGCGGGCTGTACGGTGTAGCGTTGGGTGGCGTTTTTTACGGC
GAGTCAATGTTCTACCGCCGCACTGATGCTTCAAAGGTGGCGGTGGTGTGGCTTGTGCGCCTGCTTGAATTCTGGGGATA
CCGCGTTGTGGATTGTCAGCAGACTACGACACATATGCTGCGCTTTGGCGCGCAGGAGGTGTCCCGGGCATGTTTTCTGG
AAGAGCTTGACCGCGCGCTGCGGATGCCTTTGCGGGCGGGCAGGTGGCAGATACCCGATGGATTCCGCCCGTTGCGGCGC
GCGACGCTGCCGGATGAGAATCAGTGA

Upstream 100 bases:

>100_bases
TCCGCAAGCCGGCAGCGCACGGAGCGGATGATGACCTCGGGCTCACGTTCAGATTCGAGACTTCCGCCTTGTAGCGGTAC
GGACCCGGGCAGGAGTCTGC

Downstream 100 bases:

>100_bases
GCTGTAACGCCCGCCGGTATGCCGGCGGGCGTTTTTGATGTGAAAAGGATCAGATGTTTTCGCCCACCAGAGGTTCATGC
TGGGCCAGATCATAAAAGCC

Product: leucyl/phenylalanyl-tRNA--protein transferase

Products: NA

Alternate protein names: L/F-transferase; Leucyltransferase; Phenyalanyltransferase

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MHLYALSEKLEFPDPQTASSEGLLAIGGDLSVARLVTAYANGIFPWYDDDTPILWWSPDPRCVLYPSQLHVPASLRRRIN
SGQFEVTLDTAFEAVIHACADAARPGQSGTWIVDEMIDAYMNLHEAGVAHSVEVWSRGAGDGRVLAGGLYGVALGGVFYG
ESMFYRRTDASKVAVVWLVRLLEFWGYRVVDCQQTTTHMLRFGAQEVSRACFLEELDRALRMPLRAGRWQIPDGFRPLRR
ATLPDENQ

Sequences:

>Translated_248_residues
MHLYALSEKLEFPDPQTASSEGLLAIGGDLSVARLVTAYANGIFPWYDDDTPILWWSPDPRCVLYPSQLHVPASLRRRIN
SGQFEVTLDTAFEAVIHACADAARPGQSGTWIVDEMIDAYMNLHEAGVAHSVEVWSRGAGDGRVLAGGLYGVALGGVFYG
ESMFYRRTDASKVAVVWLVRLLEFWGYRVVDCQQTTTHMLRFGAQEVSRACFLEELDRALRMPLRAGRWQIPDGFRPLRR
ATLPDENQ
>Mature_248_residues
MHLYALSEKLEFPDPQTASSEGLLAIGGDLSVARLVTAYANGIFPWYDDDTPILWWSPDPRCVLYPSQLHVPASLRRRIN
SGQFEVTLDTAFEAVIHACADAARPGQSGTWIVDEMIDAYMNLHEAGVAHSVEVWSRGAGDGRVLAGGLYGVALGGVFYG
ESMFYRRTDASKVAVVWLVRLLEFWGYRVVDCQQTTTHMLRFGAQEVSRACFLEELDRALRMPLRAGRWQIPDGFRPLRR
ATLPDENQ

Specific function: Functions in the N-end rule pathway of protein degradation where it conjugates Leu, Phe and, less efficiently, Met from aminoacyl-tRNAs to the N-termini of proteins containing an N-terminal arginine or lysine

COG id: COG2360

COG function: function code O; Leu/Phe-tRNA-protein transferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the L/F-transferase family

Homologues:

Organism=Escherichia coli, GI1787111, Length=240, Percent_Identity=45, Blast_Score=181, Evalue=4e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LFTR_DESDG (Q30ZK1)

Other databases:

- EMBL:   CP000112
- RefSeq:   YP_388590.1
- HSSP:   P0A8P1
- ProteinModelPortal:   Q30ZK1
- SMR:   Q30ZK1
- STRING:   Q30ZK1
- GeneID:   3757106
- GenomeReviews:   CP000112_GR
- KEGG:   dde:Dde_2098
- NMPDR:   fig|207559.3.peg.2246
- eggNOG:   COG2360
- HOGENOM:   HBG485363
- OMA:   GEPILWW
- ProtClustDB:   PRK00301
- BioCyc:   DDES207559:DDE_2098-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00688
- InterPro:   IPR016181
- InterPro:   IPR004616
- TIGRFAMs:   TIGR00667

Pfam domain/function: PF03588 Leu_Phe_trans; SSF55729 Acyl_CoA_acyltransferase

EC number: =2.3.2.6

Molecular weight: Translated: 27736; Mature: 27736

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHLYALSEKLEFPDPQTASSEGLLAIGGDLSVARLVTAYANGIFPWYDDDTPILWWSPDP
CCEEECCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHCCCCCCCCCCCCEEEECCCC
RCVLYPSQLHVPASLRRRINSGQFEVTLDTAFEAVIHACADAARPGQSGTWIVDEMIDAY
CEEEECCCCCCCHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
MNLHEAGVAHSVEVWSRGAGDGRVLAGGLYGVALGGVFYGESMFYRRTDASKVAVVWLVR
HHHHHHCCHHHHHHHHCCCCCCCEEECHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHH
LLEFWGYRVVDCQQTTTHMLRFGAQEVSRACFLEELDRALRMPLRAGRWQIPDGFRPLRR
HHHHHCCEEEECHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCHHHHH
ATLPDENQ
CCCCCCCC
>Mature Secondary Structure
MHLYALSEKLEFPDPQTASSEGLLAIGGDLSVARLVTAYANGIFPWYDDDTPILWWSPDP
CCEEECCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHCCCCCCCCCCCCEEEECCCC
RCVLYPSQLHVPASLRRRINSGQFEVTLDTAFEAVIHACADAARPGQSGTWIVDEMIDAY
CEEEECCCCCCCHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
MNLHEAGVAHSVEVWSRGAGDGRVLAGGLYGVALGGVFYGESMFYRRTDASKVAVVWLVR
HHHHHHCCHHHHHHHHCCCCCCCEEECHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHH
LLEFWGYRVVDCQQTTTHMLRFGAQEVSRACFLEELDRALRMPLRAGRWQIPDGFRPLRR
HHHHHCCEEEECHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCHHHHH
ATLPDENQ
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA