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The map label for this gene is nadE [H]

Identifier: 78357134

GI number: 78357134

Start: 2138561

End: 2140213

Strand: Reverse

Name: nadE [H]

Synonym: Dde_2091

Alternate gene names: 78357134

Gene position: 2140213-2138561 (Counterclockwise)

Preceding gene: 78357135

Following gene: 78357130

Centisome position: 57.37

GC content: 60.8

Gene sequence:

>1653_bases
ATGCGTATCGCTCTGTTACAACTGAACCCCGTGGTGGGTGATCTTGCAGCCAATGCGTCTGCCATTGCCGGTGCGGTGCG
AAGCGCCAGTGCAGGGGGTGCCCGGCTGTGCGTGACTTCCGAGCTGGCTTTGTGCGGGTATCCCCCCCGCGATCTTCTGC
TGATGGACGAATTTATCTCCGCCTGCCGCGGCCATCTGGACAGGCTGGCGCAGGAGCTTGCGGATTGCTGTCCGGTGCTG
GTCGGTGCTCCCGTGGCAGCAGCCGGCGATTATCCCGATACCGTGCGCAATGCGGCAGTGCTGCTGCGTGACGGCGTTGC
GGAGGTTGCAGGCTCCAAGGTGCTGCTGCCCACGTATGATGTGTTCGACGAACGCCGGTATTTCACCCCGGGGCACCGGT
GCGGTCTGGTCACCGTGGACGGATACCGTGTGGGCGTAAGCATCTGCGAAGATATATGGAATGACAAGGAGTACTGGGGA
GAGCACAGACGCTATCCCCATGATCCCGTGGCGGAACTCGCCTCGGAAGGTGTGGACCTCTTGGTGAACCTTTCGGCTTC
TCCGTTTTCGCTGGGCAAGCAGGCGCAGCGGGAGTCCATGCTGGGTGCCGTGGCGGCGCGTATTGGTGTTCCCGTGGTGT
ATGTGAATCAGGTCGGCGGTAATGACGATCTGATTTTTGCGGGCAAAAGTATGGTGATGGGCGCCGACGGCACGTTGCTG
GCCCGCGCTGCGGGGTTTGTTCCCGAGGTGTTGCTGGCTGATGCCTGCGCCGCGTCGGCCGGTGCTGTCGCACCTGACGC
TCCGGAGCCCGAGGCCCAGATATGGCAGGCTCTGGTGCTGGGTACGCGCGACTATGCTGCCAAATGCGGCTTTAAAGGTG
CGGTGCTGGGGCTTTCCGGTGGTATTGATTCGGCGCTGGTCGCCTGCATCGCCGCCGAAGCACTGGGGCCGGAAAATGTC
CTCGGCGTGATGATGCCTTCTCCGTATTCCAGCAGAGGCAGTGTGGATGATTCGGTTGCACTGGTGCAAAATCTGGGCAT
ACGCTCGCGCGTTGTGCCCATTGAACCGCTGATGCGCTCGTTTGATGCTGCTCTTTCTGAATCTTTTGCAGGGCTGGATG
CCGATGTGACAGAAGAAAATATCCAGTCGCGTATCCGTGGCAATATTCTTATGGCCATGTCCAACAAATTCGGCAAAGTG
CTGCTGACCACAGGTAACAAGTCCGAGCTGGCCGTGGGGTACTGCACCATTTACGGCGACATGGCAGGTGGGCTCGGCGT
GATAGCCGACCTGCCCAAAACCATGGTGTACGCCGTGTGCCGTTGGCTTAACAGCGTCCGCGGTTTTGAAGTGATTCCCC
GCGCTGTTCTGGAAAAGGCTCCTTCTGCGGAGCTGCGGCCCGACCAGAAGGACGTAGATTCGCTTCCTGACTATGCCGTT
CTGGATGATGTGCTGGAACGGCATATCGTGCGGAAGCAGGGGATGGCCGCCATTGTGGCGGCGGGACACCATCCTGAAAC
GGTACGCCGTATCATCGGGCTTGTCCGGAGGGCGGAATTCAAACGGCGTCAGGCCCCGCCCGGCCTCAAGGTCACTGACA
GGGCGTTCGGTTCGGGCTGGCGCATGCCGGTGGCGGCCAGATATGCTTGCTGA

Upstream 100 bases:

>100_bases
CCATTCGCGTGAAACCTTTGCAGCAGGGGCTATCCGTGCCGCAAAGTGGCTGGCGCAGAACAGACCCGGTAAACTGTATA
CCATGTCGGACATGCTGTAG

Downstream 100 bases:

>100_bases
GCTTGTGCTGCTTTTATCCCCGCTCTTTTCCGGAGCGGGGTTTTTTATTATTCGGTGCTGAACGGCATGCGCATTTCCAT
ACCGAGCAGGGAAGAATTAC

Product: NH(3)-dependent NAD(+) synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 550; Mature: 550

Protein sequence:

>550_residues
MRIALLQLNPVVGDLAANASAIAGAVRSASAGGARLCVTSELALCGYPPRDLLLMDEFISACRGHLDRLAQELADCCPVL
VGAPVAAAGDYPDTVRNAAVLLRDGVAEVAGSKVLLPTYDVFDERRYFTPGHRCGLVTVDGYRVGVSICEDIWNDKEYWG
EHRRYPHDPVAELASEGVDLLVNLSASPFSLGKQAQRESMLGAVAARIGVPVVYVNQVGGNDDLIFAGKSMVMGADGTLL
ARAAGFVPEVLLADACAASAGAVAPDAPEPEAQIWQALVLGTRDYAAKCGFKGAVLGLSGGIDSALVACIAAEALGPENV
LGVMMPSPYSSRGSVDDSVALVQNLGIRSRVVPIEPLMRSFDAALSESFAGLDADVTEENIQSRIRGNILMAMSNKFGKV
LLTTGNKSELAVGYCTIYGDMAGGLGVIADLPKTMVYAVCRWLNSVRGFEVIPRAVLEKAPSAELRPDQKDVDSLPDYAV
LDDVLERHIVRKQGMAAIVAAGHHPETVRRIIGLVRRAEFKRRQAPPGLKVTDRAFGSGWRMPVAARYAC

Sequences:

>Translated_550_residues
MRIALLQLNPVVGDLAANASAIAGAVRSASAGGARLCVTSELALCGYPPRDLLLMDEFISACRGHLDRLAQELADCCPVL
VGAPVAAAGDYPDTVRNAAVLLRDGVAEVAGSKVLLPTYDVFDERRYFTPGHRCGLVTVDGYRVGVSICEDIWNDKEYWG
EHRRYPHDPVAELASEGVDLLVNLSASPFSLGKQAQRESMLGAVAARIGVPVVYVNQVGGNDDLIFAGKSMVMGADGTLL
ARAAGFVPEVLLADACAASAGAVAPDAPEPEAQIWQALVLGTRDYAAKCGFKGAVLGLSGGIDSALVACIAAEALGPENV
LGVMMPSPYSSRGSVDDSVALVQNLGIRSRVVPIEPLMRSFDAALSESFAGLDADVTEENIQSRIRGNILMAMSNKFGKV
LLTTGNKSELAVGYCTIYGDMAGGLGVIADLPKTMVYAVCRWLNSVRGFEVIPRAVLEKAPSAELRPDQKDVDSLPDYAV
LDDVLERHIVRKQGMAAIVAAGHHPETVRRIIGLVRRAEFKRRQAPPGLKVTDRAFGSGWRMPVAARYAC
>Mature_550_residues
MRIALLQLNPVVGDLAANASAIAGAVRSASAGGARLCVTSELALCGYPPRDLLLMDEFISACRGHLDRLAQELADCCPVL
VGAPVAAAGDYPDTVRNAAVLLRDGVAEVAGSKVLLPTYDVFDERRYFTPGHRCGLVTVDGYRVGVSICEDIWNDKEYWG
EHRRYPHDPVAELASEGVDLLVNLSASPFSLGKQAQRESMLGAVAARIGVPVVYVNQVGGNDDLIFAGKSMVMGADGTLL
ARAAGFVPEVLLADACAASAGAVAPDAPEPEAQIWQALVLGTRDYAAKCGFKGAVLGLSGGIDSALVACIAAEALGPENV
LGVMMPSPYSSRGSVDDSVALVQNLGIRSRVVPIEPLMRSFDAALSESFAGLDADVTEENIQSRIRGNILMAMSNKFGKV
LLTTGNKSELAVGYCTIYGDMAGGLGVIADLPKTMVYAVCRWLNSVRGFEVIPRAVLEKAPSAELRPDQKDVDSLPDYAV
LDDVLERHIVRKQGMAAIVAAGHHPETVRRIIGLVRRAEFKRRQAPPGLKVTDRAFGSGWRMPVAARYAC

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

None

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 58287; Mature: 58287

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIALLQLNPVVGDLAANASAIAGAVRSASAGGARLCVTSELALCGYPPRDLLLMDEFIS
CEEEEEECCCHHHHHHCCHHHHHHHHHHCCCCCCEEEEECCHHHCCCCCHHHHHHHHHHH
ACRGHLDRLAQELADCCPVLVGAPVAAAGDYPDTVRNAAVLLRDGVAEVAGSKVLLPTYD
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCHHHHCCCEEEECCHH
VFDERRYFTPGHRCGLVTVDGYRVGVSICEDIWNDKEYWGEHRRYPHDPVAELASEGVDL
HHCCCCCCCCCCCEEEEEECCEEHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHCCCEE
LVNLSASPFSLGKQAQRESMLGAVAARIGVPVVYVNQVGGNDDLIFAGKSMVMGADGTLL
EEECCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEECCCEEECCCCHHH
ARAAGFVPEVLLADACAASAGAVAPDAPEPEAQIWQALVLGTRDYAAKCGFKGAVLGLSG
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHCCCCCEEEEECC
GIDSALVACIAAEALGPENVLGVMMPSPYSSRGSVDDSVALVQNLGIRSRVVPIEPLMRS
CHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHH
FDAALSESFAGLDADVTEENIQSRIRGNILMAMSNKFGKVLLTTGNKSELAVGYCTIYGD
HHHHHHHHHCCCCCCCCHHHHHHHHCCCEEEEECCCCCEEEEEECCCCCEEEEEEEEEEC
MAGGLGVIADLPKTMVYAVCRWLNSVRGFEVIPRAVLEKAPSAELRPDQKDVDSLPDYAV
CCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCHHHHHCCCCHHH
LDDVLERHIVRKQGMAAIVAAGHHPETVRRIIGLVRRAEFKRRQAPPGLKVTDRAFGSGW
HHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHCCCCC
RMPVAARYAC
CCCCCCCCCC
>Mature Secondary Structure
MRIALLQLNPVVGDLAANASAIAGAVRSASAGGARLCVTSELALCGYPPRDLLLMDEFIS
CEEEEEECCCHHHHHHCCHHHHHHHHHHCCCCCCEEEEECCHHHCCCCCHHHHHHHHHHH
ACRGHLDRLAQELADCCPVLVGAPVAAAGDYPDTVRNAAVLLRDGVAEVAGSKVLLPTYD
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCHHHHCCCEEEECCHH
VFDERRYFTPGHRCGLVTVDGYRVGVSICEDIWNDKEYWGEHRRYPHDPVAELASEGVDL
HHCCCCCCCCCCCEEEEEECCEEHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHCCCEE
LVNLSASPFSLGKQAQRESMLGAVAARIGVPVVYVNQVGGNDDLIFAGKSMVMGADGTLL
EEECCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEECCCEEECCCCHHH
ARAAGFVPEVLLADACAASAGAVAPDAPEPEAQIWQALVLGTRDYAAKCGFKGAVLGLSG
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHCCCCCEEEEECC
GIDSALVACIAAEALGPENVLGVMMPSPYSSRGSVDDSVALVQNLGIRSRVVPIEPLMRS
CHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHH
FDAALSESFAGLDADVTEENIQSRIRGNILMAMSNKFGKVLLTTGNKSELAVGYCTIYGD
HHHHHHHHHCCCCCCCCHHHHHHHHCCCEEEEECCCCCEEEEEECCCCCEEEEEEEEEEC
MAGGLGVIADLPKTMVYAVCRWLNSVRGFEVIPRAVLEKAPSAELRPDQKDVDSLPDYAV
CCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCHHHHHCCCCHHH
LDDVLERHIVRKQGMAAIVAAGHHPETVRRIIGLVRRAEFKRRQAPPGLKVTDRAFGSGW
HHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHCCCCC
RMPVAARYAC
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]