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The map label for this gene is cca [H]

Identifier: 78357126

GI number: 78357126

Start: 2130032

End: 2131138

Strand: Direct

Name: cca [H]

Synonym: Dde_2083

Alternate gene names: 78357126

Gene position: 2130032-2131138 (Clockwise)

Preceding gene: 78357122

Following gene: 78357127

Centisome position: 57.1

GC content: 60.07

Gene sequence:

>1107_bases
ATGGTACCGGAACCTATGCAGACATTCCTTGTAGGCGGTGCGGTCCGCGACATGCTGCTGGGCAGAACACCCCGCGAATA
CGATTATGTATTTTCCGGGTCGCTTGAAGAGTTTCTGCTGCGGCATAAAACAGCCCGCAGGGCCGGTACACAGTTTTCCA
TAGCCATCTACAACGGGCTGGAATTCGCCCCCATGCGCGGAAAGACCATACATGAGGATCTGGCTTCGCGCGACTTCACC
GTCAATGCCATGGCGCTTGACGCCGACGGCAGACTGTTTGCCCATCCTGAAGCCCTGCGGGATCTGGCACTGCACAGACT
GTCGCCCGCTTCATCTCATGCCCTTGCGGACGATCCGGTACGGCTGTTCAGAGCCGCAAGGTTCACCGCCACCCTGCCCG
GTTTTCACATTTCCGCAGACCTGAAGCGCGCCATGCGTGCAGAAGCCGGTTCCGGCAAAATAATGGATGCAGCTCCGGAA
CGTATCGGCAGCGAAATTCACAAAGTGCTGCACAGTGCACGGCCGGGCAACTTTCTGCGGACACTCAACGAGGCTGACTG
CCTGACCGCATGGCTGCAGGAGTTCGCCCCTGCTGAAGAGGATGCGGCCGCGCTGCACCGTACGGCAGATATCATGGACA
GAGCGGCAGGCGTGCCCATGGCTGTATGGATGGCTCTGTGCCACGATTTGAACACAGGCGCAACGCAAAGTCCGTTACCC
GCCGCAGCCCCAACGCCGGTGCGGAAACAGCCGCAGCACCATGCGGCAGCTCCGGCAGCCCGCAGATTCGCCATGCGCAT
CAAGGCCCCGAATTCCTTTGTACGCGCTGCCGAGGTGGCCGCTTCATGCCACAAAGAAGGCATGCATTACCCCGCACTTA
AGGCTGAAAGCCGTGTTTCGCTGCTCATGCGGCTGCATCTCGCCGCATTACTTGATGATTTCTTTACCTTCATCCTAGCT
GTCACCGGCAGTGACTTTCGCAGACCGGCAGCGGAAGAACTCGATATCCTGCTGCGGGTAAAACTGCCCCCGGACCAGCA
GGGACGGGGGCCCGAATCGGGACGCCTGCTGCACCGCATGCGCTGCGAGGCACTGCAGAGCCGTTGA

Upstream 100 bases:

>100_bases
TTTGAAAGAATGTACACGGGAAAGGACATTACTACCTCATTCATACACGGAAAAAACACCCTGCGCGCAACGCCGCGGCC
CTTTTGTGCCGCGGCGATAT

Downstream 100 bases:

>100_bases
ACAGAGTGCTGCCCCGGCGCAATGCCGCCTCCGTACGCTGCCAAAGACAGCAATACCCCAAACGGCAGGTACCGGTCCGC
AGAAAAGCCAGCTGAAAAGA

Product: tRNA nucleotidyltransferase

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase [H]

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MVPEPMQTFLVGGAVRDMLLGRTPREYDYVFSGSLEEFLLRHKTARRAGTQFSIAIYNGLEFAPMRGKTIHEDLASRDFT
VNAMALDADGRLFAHPEALRDLALHRLSPASSHALADDPVRLFRAARFTATLPGFHISADLKRAMRAEAGSGKIMDAAPE
RIGSEIHKVLHSARPGNFLRTLNEADCLTAWLQEFAPAEEDAAALHRTADIMDRAAGVPMAVWMALCHDLNTGATQSPLP
AAAPTPVRKQPQHHAAAPAARRFAMRIKAPNSFVRAAEVAASCHKEGMHYPALKAESRVSLLMRLHLAALLDDFFTFILA
VTGSDFRRPAAEELDILLRVKLPPDQQGRGPESGRLLHRMRCEALQSR

Sequences:

>Translated_368_residues
MVPEPMQTFLVGGAVRDMLLGRTPREYDYVFSGSLEEFLLRHKTARRAGTQFSIAIYNGLEFAPMRGKTIHEDLASRDFT
VNAMALDADGRLFAHPEALRDLALHRLSPASSHALADDPVRLFRAARFTATLPGFHISADLKRAMRAEAGSGKIMDAAPE
RIGSEIHKVLHSARPGNFLRTLNEADCLTAWLQEFAPAEEDAAALHRTADIMDRAAGVPMAVWMALCHDLNTGATQSPLP
AAAPTPVRKQPQHHAAAPAARRFAMRIKAPNSFVRAAEVAASCHKEGMHYPALKAESRVSLLMRLHLAALLDDFFTFILA
VTGSDFRRPAAEELDILLRVKLPPDQQGRGPESGRLLHRMRCEALQSR
>Mature_368_residues
MVPEPMQTFLVGGAVRDMLLGRTPREYDYVFSGSLEEFLLRHKTARRAGTQFSIAIYNGLEFAPMRGKTIHEDLASRDFT
VNAMALDADGRLFAHPEALRDLALHRLSPASSHALADDPVRLFRAARFTATLPGFHISADLKRAMRAEAGSGKIMDAAPE
RIGSEIHKVLHSARPGNFLRTLNEADCLTAWLQEFAPAEEDAAALHRTADIMDRAAGVPMAVWMALCHDLNTGATQSPLP
AAAPTPVRKQPQHHAAAPAARRFAMRIKAPNSFVRAAEVAASCHKEGMHYPALKAESRVSLLMRLHLAALLDDFFTFILA
VTGSDFRRPAAEELDILLRVKLPPDQQGRGPESGRLLHRMRCEALQSR

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789436, Length=336, Percent_Identity=28.2738095238095, Blast_Score=103, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 40500; Mature: 40500

Theoretical pI: Translated: 8.95; Mature: 8.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVPEPMQTFLVGGAVRDMLLGRTPREYDYVFSGSLEEFLLRHKTARRAGTQFSIAIYNGL
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCEECCCHHHHHHHHHHHHHCCCEEEEEEECCC
EFAPMRGKTIHEDLASRDFTVNAMALDADGRLFAHPEALRDLALHRLSPASSHALADDPV
EECCCCCCHHHHHHHCCCCEEEEEEECCCCCEEECHHHHHHHHHHHCCCCCCCCCCCCHH
RLFRAARFTATLPGFHISADLKRAMRAEAGSGKIMDAAPERIGSEIHKVLHSARPGNFLR
HHHHHHHHHHCCCCCEEHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHH
TLNEADCLTAWLQEFAPAEEDAAALHRTADIMDRAAGVPMAVWMALCHDLNTGATQSPLP
HCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCC
AAAPTPVRKQPQHHAAAPAARRFAMRIKAPNSFVRAAEVAASCHKEGMHYPALKAESRVS
CCCCCCCCCCCCHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
LLMRLHLAALLDDFFTFILAVTGSDFRRPAAEELDILLRVKLPPDQQGRGPESGRLLHRM
HHHHHHHHHHHHHHHHHHHHHCCCHHCCCCHHCCCEEEEEECCCCCCCCCCCHHHHHHHH
RCEALQSR
HHHHHHCC
>Mature Secondary Structure
MVPEPMQTFLVGGAVRDMLLGRTPREYDYVFSGSLEEFLLRHKTARRAGTQFSIAIYNGL
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCEECCCHHHHHHHHHHHHHCCCEEEEEEECCC
EFAPMRGKTIHEDLASRDFTVNAMALDADGRLFAHPEALRDLALHRLSPASSHALADDPV
EECCCCCCHHHHHHHCCCCEEEEEEECCCCCEEECHHHHHHHHHHHCCCCCCCCCCCCHH
RLFRAARFTATLPGFHISADLKRAMRAEAGSGKIMDAAPERIGSEIHKVLHSARPGNFLR
HHHHHHHHHHCCCCCEEHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHH
TLNEADCLTAWLQEFAPAEEDAAALHRTADIMDRAAGVPMAVWMALCHDLNTGATQSPLP
HCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCC
AAAPTPVRKQPQHHAAAPAARRFAMRIKAPNSFVRAAEVAASCHKEGMHYPALKAESRVS
CCCCCCCCCCCCHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
LLMRLHLAALLDDFFTFILAVTGSDFRRPAAEELDILLRVKLPPDQQGRGPESGRLLHRM
HHHHHHHHHHHHHHHHHHHHHCCCHHCCCCHHCCCEEEEEECCCCCCCCCCCHHHHHHHH
RCEALQSR
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA