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The map label for this gene is cca [H]

Identifier: 78357015

GI number: 78357015

Start: 2015901

End: 2018597

Strand: Direct

Name: cca [H]

Synonym: Dde_1972

Alternate gene names: 78357015

Gene position: 2015901-2018597 (Clockwise)

Preceding gene: 78357014

Following gene: 78357016

Centisome position: 54.04

GC content: 55.21

Gene sequence:

>2697_bases
ATGACGACTTCACCCCCTCTTATAGCCGCCCCCGTGGTTGTGACCGCCCATGCCAATGCCGATTTTGACGCGCTGGCTTC
CATTGTCGCCGCAGGCAGACTATACCCCGGTGCCACACTGCTTTTCCCCGGCAGCCAGGAACGCACGCTGAGACACTTCT
TCATCAAAAGCGCCACCTATCTTTTCAACTTCCGGCAGGCAAAGGACATTGATGTAACCAGCGTTGAAAAGCTGGTCATA
GTCGACACCCGCCAGCACTCAAGGGTGCCTCATGTTCATGCGATACTTGAACGTCCGGAAGTTGAAGTACACCTTTATGA
CCACCACCCCGACACGGACGAGGACATACAGGGAAGCCTTGTGACGGTCCGCCAGTGGGGAGCCACAGCCACCATCCTGA
CCACCGAAATACAACAGGCCGGACTCGCGCTTACTCCGGATGAAGCTACCATCATCGGGCTGGGTATTTTTGAGGATACC
GGCTCCTTCACCTTTGCCTCAACCACCGAACATGACTTTGCCGCCGCAGGCTGGCTGAAAACGCAGGGAATGGACCTGAA
CACCATTTCTGAACTGCTTACCCGTGATCTGACCAGCGAACAGATATCCGCCCTTAATGAACTGCTTGAAACAGCTCACA
CGCACGACATCAACGGTGTTCCCGTTGTGATAGCCGAAGCCAGCATGGATGAATACATGGGCGACTTTGCCCTGCTGGCC
CACAAGCTGATGGATATGGAAAACATCAGCGTGCTCTTTGCTTTGGGACGCATGGAAGACAGGGTACAGATAGTGGCCCG
CGCACGCTCTTCAGACGTTGATGTGGGAGCCATATGCTCCGGTTTCGGCGGTGGCGGACACACATACGCAGCTTCAGCAT
CCGTCAAATTCAAAACACTTTCTCAAGTAAAGGATGAACTTTTTACGCTTCTCTATTCCGCGGTCAACAAACAGCTGACT
GTCGGCTCCATCATGTCTGCGCCGGTCATTGCCGAACCGGAAACGGCAACCATTGCCAAAGCGGCGGAAACCATGCAGCG
CTTCGGCCTGAAGGCCGTGCCGGTGGTGATGCCGGGTACCGCACGCTGTACGGGCTACATAGAATACCAGACCGCCACCC
GCGCAGTCGCTCACGGGCTGGGCGCAGCCCCCGTGACCGACTACATGAACCGCCATGTGCATACTGCATCGCCCGAAGAC
GACCTGCACACCATGATGGAAATAATAGTAGGTCAGCGTCAGCGTCTGGTGCCCGTGGTGGACGAAAACGGCAACGCCAT
CGGAGTGATCACCCGGACGGATCTCATCAATACAATAATTGAAGAGCCCGCCCGGATTCCAGAAACGCTTCAGCCCGACA
GAAAGCGTGACCGGAACATCCGTTCGCTGATACGTGAACGGCTGCCCCGCATGCACTGCGGCTGGCTGGAAGCCGCAGGA
AAGCTGGGGGATGTTTCAGGGGTCGCTGTATACGCTGTTGGCGGCTTTATACGCGACCTGCTGCTGGGCAGGCCCAATCT
GGACCTTGATCTGGTTGTTGAAGGAGACGGGATAACATTTGCCCGCAGACTGGCAAAACAACTCAACGGACGCATACGCG
AGCACCAGAAGTTCAAAACCGCAGTTGTAATCTTTGATGACGAAAACGGTGTAGAAAGCCGGATAGATGTGGCTACGGCC
AGACTGGAATATTATGAATACCCTGCGGCCCTGCCCACTGTAGAGCTATCTTCCATCAAGATGGATCTCTTCCGGCGGGA
TTTCACCATTAACGCTCTGGCAGTTCAGCTCAATGAAAGCAGTTTCGGCAGACTGGTGGACTTTTTCGGCTCGCAGCAGG
ATATCAAAGACAAGCGTATCCGGGTACTCCATTCGCTGAGCTTCGTGGAAGACCCCACCAGGATTCTTAGAGCCATCCGC
TTTGAAAAACGATACAATTTTGCTATAGGCCCGCAGACTTCACGGCTCATCAAAAACGCTCTCGGTCTCGGACTCATGGA
AAAGTTGTCCGGTTCACGCCTGTTCCACGAACTGCGCCTTATCACTGAAGAGGCCTCCCCGCTTGCCTGCTTTAAAAGGA
TGGAAGAGTTCGACCTGTTGCGCAGTGTGCACCCGGTGCTGGCACTGAGCTATGGCAAAGAACAAATTATCGCCGACGTG
GAAAAGGTGCTCGGCTGGCACAGGCTGCTGTATCAGGACCCTGCGGCGGAAGCATGGACTGTGCATCTGCTTGCCTTGTG
CCACAATGCCAAATATGCCGACGTGCGCGATCTGCTTAAAAGACTGTATCTGCCGCGTAAGCAGAGCCGTGATTTTATGC
TGCTGCGTGAAAACACCCGTGAAGCGGCGGCCCTGCTTTCCACATGGCTGAAAAAAGACCGTTCCATGAGCGGACTTTAC
AACATCCTGCATTCTTTGCCGCCCGAAGGCCTTCTTTACATCATGGCAAGAACACGTGTGGATGAAATCCGCAAATTCGT
CTCGCATTACCTCACCAAGCTGCGGGATATCCGGACAGACATCAGCGGCGAAGACCTGCAGGCCATGGGCGGGCACCCCG
GCCCTTGCTTCGGCAAGGTGCTGAACCATGTGCTGGCAGCCAAACTGGACGGAGCAGCCCACAGCCGTAAAGAACAGCTG
GAGCTGGCCCACAGCATGCTGCGACGCTATAATTACGGCCGCGACGAACAGCAATAG

Upstream 100 bases:

>100_bases
ACCGAAATTTACACCCACGTAGAAACAGAACGCCTGCGCAGGATACACAAACAGTTCCATCCGCGCTCCAAAATCAAAAT
CTAGCGACGGTCAGAAACGT

Downstream 100 bases:

>100_bases
CAGCGCGGCACCGAGCACTAATCACTGCAAGGATTTGATTGCGTTCAACGTATTGACTGTGTAGGTAGTTTCAACACCGG
TATCATACGGCACAGGCGGC

Product: hypothetical protein

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase [H]

Number of amino acids: Translated: 898; Mature: 897

Protein sequence:

>898_residues
MTTSPPLIAAPVVVTAHANADFDALASIVAAGRLYPGATLLFPGSQERTLRHFFIKSATYLFNFRQAKDIDVTSVEKLVI
VDTRQHSRVPHVHAILERPEVEVHLYDHHPDTDEDIQGSLVTVRQWGATATILTTEIQQAGLALTPDEATIIGLGIFEDT
GSFTFASTTEHDFAAAGWLKTQGMDLNTISELLTRDLTSEQISALNELLETAHTHDINGVPVVIAEASMDEYMGDFALLA
HKLMDMENISVLFALGRMEDRVQIVARARSSDVDVGAICSGFGGGGHTYAASASVKFKTLSQVKDELFTLLYSAVNKQLT
VGSIMSAPVIAEPETATIAKAAETMQRFGLKAVPVVMPGTARCTGYIEYQTATRAVAHGLGAAPVTDYMNRHVHTASPED
DLHTMMEIIVGQRQRLVPVVDENGNAIGVITRTDLINTIIEEPARIPETLQPDRKRDRNIRSLIRERLPRMHCGWLEAAG
KLGDVSGVAVYAVGGFIRDLLLGRPNLDLDLVVEGDGITFARRLAKQLNGRIREHQKFKTAVVIFDDENGVESRIDVATA
RLEYYEYPAALPTVELSSIKMDLFRRDFTINALAVQLNESSFGRLVDFFGSQQDIKDKRIRVLHSLSFVEDPTRILRAIR
FEKRYNFAIGPQTSRLIKNALGLGLMEKLSGSRLFHELRLITEEASPLACFKRMEEFDLLRSVHPVLALSYGKEQIIADV
EKVLGWHRLLYQDPAAEAWTVHLLALCHNAKYADVRDLLKRLYLPRKQSRDFMLLRENTREAAALLSTWLKKDRSMSGLY
NILHSLPPEGLLYIMARTRVDEIRKFVSHYLTKLRDIRTDISGEDLQAMGGHPGPCFGKVLNHVLAAKLDGAAHSRKEQL
ELAHSMLRRYNYGRDEQQ

Sequences:

>Translated_898_residues
MTTSPPLIAAPVVVTAHANADFDALASIVAAGRLYPGATLLFPGSQERTLRHFFIKSATYLFNFRQAKDIDVTSVEKLVI
VDTRQHSRVPHVHAILERPEVEVHLYDHHPDTDEDIQGSLVTVRQWGATATILTTEIQQAGLALTPDEATIIGLGIFEDT
GSFTFASTTEHDFAAAGWLKTQGMDLNTISELLTRDLTSEQISALNELLETAHTHDINGVPVVIAEASMDEYMGDFALLA
HKLMDMENISVLFALGRMEDRVQIVARARSSDVDVGAICSGFGGGGHTYAASASVKFKTLSQVKDELFTLLYSAVNKQLT
VGSIMSAPVIAEPETATIAKAAETMQRFGLKAVPVVMPGTARCTGYIEYQTATRAVAHGLGAAPVTDYMNRHVHTASPED
DLHTMMEIIVGQRQRLVPVVDENGNAIGVITRTDLINTIIEEPARIPETLQPDRKRDRNIRSLIRERLPRMHCGWLEAAG
KLGDVSGVAVYAVGGFIRDLLLGRPNLDLDLVVEGDGITFARRLAKQLNGRIREHQKFKTAVVIFDDENGVESRIDVATA
RLEYYEYPAALPTVELSSIKMDLFRRDFTINALAVQLNESSFGRLVDFFGSQQDIKDKRIRVLHSLSFVEDPTRILRAIR
FEKRYNFAIGPQTSRLIKNALGLGLMEKLSGSRLFHELRLITEEASPLACFKRMEEFDLLRSVHPVLALSYGKEQIIADV
EKVLGWHRLLYQDPAAEAWTVHLLALCHNAKYADVRDLLKRLYLPRKQSRDFMLLRENTREAAALLSTWLKKDRSMSGLY
NILHSLPPEGLLYIMARTRVDEIRKFVSHYLTKLRDIRTDISGEDLQAMGGHPGPCFGKVLNHVLAAKLDGAAHSRKEQL
ELAHSMLRRYNYGRDEQQ
>Mature_897_residues
TTSPPLIAAPVVVTAHANADFDALASIVAAGRLYPGATLLFPGSQERTLRHFFIKSATYLFNFRQAKDIDVTSVEKLVIV
DTRQHSRVPHVHAILERPEVEVHLYDHHPDTDEDIQGSLVTVRQWGATATILTTEIQQAGLALTPDEATIIGLGIFEDTG
SFTFASTTEHDFAAAGWLKTQGMDLNTISELLTRDLTSEQISALNELLETAHTHDINGVPVVIAEASMDEYMGDFALLAH
KLMDMENISVLFALGRMEDRVQIVARARSSDVDVGAICSGFGGGGHTYAASASVKFKTLSQVKDELFTLLYSAVNKQLTV
GSIMSAPVIAEPETATIAKAAETMQRFGLKAVPVVMPGTARCTGYIEYQTATRAVAHGLGAAPVTDYMNRHVHTASPEDD
LHTMMEIIVGQRQRLVPVVDENGNAIGVITRTDLINTIIEEPARIPETLQPDRKRDRNIRSLIRERLPRMHCGWLEAAGK
LGDVSGVAVYAVGGFIRDLLLGRPNLDLDLVVEGDGITFARRLAKQLNGRIREHQKFKTAVVIFDDENGVESRIDVATAR
LEYYEYPAALPTVELSSIKMDLFRRDFTINALAVQLNESSFGRLVDFFGSQQDIKDKRIRVLHSLSFVEDPTRILRAIRF
EKRYNFAIGPQTSRLIKNALGLGLMEKLSGSRLFHELRLITEEASPLACFKRMEEFDLLRSVHPVLALSYGKEQIIADVE
KVLGWHRLLYQDPAAEAWTVHLLALCHNAKYADVRDLLKRLYLPRKQSRDFMLLRENTREAAALLSTWLKKDRSMSGLYN
ILHSLPPEGLLYIMARTRVDEIRKFVSHYLTKLRDIRTDISGEDLQAMGGHPGPCFGKVLNHVLAAKLDGAAHSRKEQLE
LAHSMLRRYNYGRDEQQ

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081691, Length=255, Percent_Identity=27.843137254902, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1789436, Length=235, Percent_Identity=29.3617021276596, Blast_Score=73, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6321016, Length=222, Percent_Identity=27.9279279279279, Blast_Score=72, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 100068; Mature: 99937

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTSPPLIAAPVVVTAHANADFDALASIVAAGRLYPGATLLFPGSQERTLRHFFIKSATY
CCCCCCCEEECEEEEEECCCCHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHH
LFNFRQAKDIDVTSVEKLVIVDTRQHSRVPHVHAILERPEVEVHLYDHHPDTDEDIQGSL
HEECCCCCCCCHHCCCEEEEEECCCCCCCCHHHHHHCCCCEEEEEECCCCCCCCCCCCCE
VTVRQWGATATILTTEIQQAGLALTPDEATIIGLGIFEDTGSFTFASTTEHDFAAAGWLK
EEEEECCCEEEEEEHHHHHCCCEECCCCCEEEEEEEEECCCCEEEECCCCCCHHHHCCCC
TQGMDLNTISELLTRDLTSEQISALNELLETAHTHDINGVPVVIAEASMDEYMGDFALLA
CCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHH
HKLMDMENISVLFALGRMEDRVQIVARARSSDVDVGAICSGFGGGGHTYAASASVKFKTL
HHHHCCCCCHHEEEHHCCHHHHHHHHHHCCCCCCHHHHHCCCCCCCCEEEECCCEEHHHH
SQVKDELFTLLYSAVNKQLTVGSIMSAPVIAEPETATIAKAAETMQRFGLKAVPVVMPGT
HHHHHHHHHHHHHHHCCCEEEHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCC
ARCTGYIEYQTATRAVAHGLGAAPVTDYMNRHVHTASPEDDLHTMMEIIVGQRQRLVPVV
CCEEEEEEEHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCEEE
DENGNAIGVITRTDLINTIIEEPARIPETLQPDRKRDRNIRSLIRERLPRMHCGWLEAAG
ECCCCEEEEEEHHHHHHHHHHCHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHCC
KLGDVSGVAVYAVGGFIRDLLLGRPNLDLDLVVEGDGITFARRLAKQLNGRIREHQKFKT
CCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHCCHHHHHHCEEE
AVVIFDDENGVESRIDVATARLEYYEYPAALPTVELSSIKMDLFRRDFTINALAVQLNES
EEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHCCCEEEEEEEEECCC
SFGRLVDFFGSQQDIKDKRIRVLHSLSFVEDPTRILRAIRFEKRYNFAIGPQTSRLIKNA
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCEEECCCHHHHHHHH
LGLGLMEKLSGSRLFHELRLITEEASPLACFKRMEEFDLLRSVHPVLALSYGKEQIIADV
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
EKVLGWHRLLYQDPAAEAWTVHLLALCHNAKYADVRDLLKRLYLPRKQSRDFMLLRENTR
HHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCEEEEECCHH
EAAALLSTWLKKDRSMSGLYNILHSLPPEGLLYIMARTRVDEIRKFVSHYLTKLRDIRTD
HHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCC
ISGEDLQAMGGHPGPCFGKVLNHVLAAKLDGAAHSRKEQLELAHSMLRRYNYGRDEQQ
CCCCCHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TTSPPLIAAPVVVTAHANADFDALASIVAAGRLYPGATLLFPGSQERTLRHFFIKSATY
CCCCCCEEECEEEEEECCCCHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHH
LFNFRQAKDIDVTSVEKLVIVDTRQHSRVPHVHAILERPEVEVHLYDHHPDTDEDIQGSL
HEECCCCCCCCHHCCCEEEEEECCCCCCCCHHHHHHCCCCEEEEEECCCCCCCCCCCCCE
VTVRQWGATATILTTEIQQAGLALTPDEATIIGLGIFEDTGSFTFASTTEHDFAAAGWLK
EEEEECCCEEEEEEHHHHHCCCEECCCCCEEEEEEEEECCCCEEEECCCCCCHHHHCCCC
TQGMDLNTISELLTRDLTSEQISALNELLETAHTHDINGVPVVIAEASMDEYMGDFALLA
CCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHH
HKLMDMENISVLFALGRMEDRVQIVARARSSDVDVGAICSGFGGGGHTYAASASVKFKTL
HHHHCCCCCHHEEEHHCCHHHHHHHHHHCCCCCCHHHHHCCCCCCCCEEEECCCEEHHHH
SQVKDELFTLLYSAVNKQLTVGSIMSAPVIAEPETATIAKAAETMQRFGLKAVPVVMPGT
HHHHHHHHHHHHHHHCCCEEEHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCC
ARCTGYIEYQTATRAVAHGLGAAPVTDYMNRHVHTASPEDDLHTMMEIIVGQRQRLVPVV
CCEEEEEEEHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCEEE
DENGNAIGVITRTDLINTIIEEPARIPETLQPDRKRDRNIRSLIRERLPRMHCGWLEAAG
ECCCCEEEEEEHHHHHHHHHHCHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHCC
KLGDVSGVAVYAVGGFIRDLLLGRPNLDLDLVVEGDGITFARRLAKQLNGRIREHQKFKT
CCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHCCHHHHHHCEEE
AVVIFDDENGVESRIDVATARLEYYEYPAALPTVELSSIKMDLFRRDFTINALAVQLNES
EEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHCCCEEEEEEEEECCC
SFGRLVDFFGSQQDIKDKRIRVLHSLSFVEDPTRILRAIRFEKRYNFAIGPQTSRLIKNA
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCEEECCCHHHHHHHH
LGLGLMEKLSGSRLFHELRLITEEASPLACFKRMEEFDLLRSVHPVLALSYGKEQIIADV
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
EKVLGWHRLLYQDPAAEAWTVHLLALCHNAKYADVRDLLKRLYLPRKQSRDFMLLRENTR
HHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCEEEEECCHH
EAAALLSTWLKKDRSMSGLYNILHSLPPEGLLYIMARTRVDEIRKFVSHYLTKLRDIRTD
HHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCC
ISGEDLQAMGGHPGPCFGKVLNHVLAAKLDGAAHSRKEQLELAHSMLRRYNYGRDEQQ
CCCCCHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA