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The map label for this gene is dapL

Identifier: 78357013

GI number: 78357013

Start: 2013568

End: 2014734

Strand: Reverse

Name: dapL

Synonym: Dde_1970

Alternate gene names: 78357013

Gene position: 2014734-2013568 (Counterclockwise)

Preceding gene: 78357025

Following gene: 78357012

Centisome position: 54.01

GC content: 54.41

Gene sequence:

>1167_bases
ATGACCCAGTTTACACTCGCTGACCGGCTTGCGACGCTTCCTCCCTATCTTTTCGCACAGATAGACAAGGTTAAAGCGGA
AGTTGCCGCCCGGGGCGTGGATATCATCAGCCTTGGCATAGGCGACCCCGATATGCCCACACCGGACTTCGTCATCGAAG
CCCTGAAGAAGGCGGCTGAAAAACCGGCAAATCATCAGTACCCCTCGTATACCGGTATGCTGGCTTTCCGTCAGGAAGTG
GCCAACTGGTACAAGCGGCGGTATGCGGTGGAACTTGACCCGAAAACCGAAGTGCTGACGCTTATCGGTTCCAAGGAAGG
CATTGCGCATTTTCCCACAGCCTTTGTCAATCCGGGGGATCTGGTACTGGTGTGCCCGCCTTGCTACCCCGTGTACGCCA
TTGCTTCCCGCTTTATGGGTGGGGTGGTGCAGGAACTTCCGCTGTTGGAAGAAAATGATTTCCTGCCCGATCTCGATGCC
GTTGATGAAGCCACATGGGAAAAAGCCCGCTGCATTTTTGTGAACTATCCCAATAACCCCACTGCAGCTATGGCACCGCG
CAGCTTTTTTGAAAAGCTGATCGGCATTGCCAGAAAGCATAATGTTATTGTGGTGCACGATGCCGCTTACACCGAGATGT
ATTACAACGAGAACAACCGGCCTCTTTCCATTATGGAAATTCCCGGTGCCATGGATGTGGCCATAGAGTTCAACTCGCTT
TCCAAGCCCTACAACATGACGGGCTGGCGCATCGCCATGGCAGTGGGTAATGCCTCTCTGGTGGCCGGTCTGGGCAAAGT
CAAGGAAAACATGGACTCGGGCGCTTTTCAGGCCGTTCAGGAGGCCGCCATCGTGGCCTTGCGCGACGGTGACGCTTTTC
TTGCAGAAATCCGTGACATTTACCGCAAGCGTCGCGATACCGTTATTGCCGCATTGAATAAAATCGGCATTACCTGCCGT
GTGCCCGAAGCCTCACTTTATGTTTGGGCCAGAGTGCCCGAAGGTTATACCTCTTCGGACTTTGTGACCCGCGTGTTGCA
GGAGACCGGCGTGGTGATGACACCCGGCAACGGTTTTGGTGCGGCAGGCGAAGGGTATTTCCGTATTTCTCTTACAGTCA
ATGACGAGCGACTTGAGGAGGCCGTATCCCGAATCGCCAGCCTGTAA

Upstream 100 bases:

>100_bases
AGCCGGAACGCCCGGACAGTTACGGCATGAACAGAAAACAGGGCTTATGGTAGAGCCTTGAGATACCGGCGGGGATGAAA
ATCCCAAGGAGACAACACGA

Downstream 100 bases:

>100_bases
CCGTTTATATCTGCCTTGGCTCCAATCAAGGCGATACAGATGCAAATCTGGAAAGGGCAGTGGCTGCCATTGCAGCCCTG
TCCGGCCTTACGGTGGAAAA

Product: LL-diaminopimelate aminotransferase

Products: NA

Alternate protein names: DAP-AT; DAP-aminotransferase; LL-DAP-aminotransferase

Number of amino acids: Translated: 388; Mature: 387

Protein sequence:

>388_residues
MTQFTLADRLATLPPYLFAQIDKVKAEVAARGVDIISLGIGDPDMPTPDFVIEALKKAAEKPANHQYPSYTGMLAFRQEV
ANWYKRRYAVELDPKTEVLTLIGSKEGIAHFPTAFVNPGDLVLVCPPCYPVYAIASRFMGGVVQELPLLEENDFLPDLDA
VDEATWEKARCIFVNYPNNPTAAMAPRSFFEKLIGIARKHNVIVVHDAAYTEMYYNENNRPLSIMEIPGAMDVAIEFNSL
SKPYNMTGWRIAMAVGNASLVAGLGKVKENMDSGAFQAVQEAAIVALRDGDAFLAEIRDIYRKRRDTVIAALNKIGITCR
VPEASLYVWARVPEGYTSSDFVTRVLQETGVVMTPGNGFGAAGEGYFRISLTVNDERLEEAVSRIASL

Sequences:

>Translated_388_residues
MTQFTLADRLATLPPYLFAQIDKVKAEVAARGVDIISLGIGDPDMPTPDFVIEALKKAAEKPANHQYPSYTGMLAFRQEV
ANWYKRRYAVELDPKTEVLTLIGSKEGIAHFPTAFVNPGDLVLVCPPCYPVYAIASRFMGGVVQELPLLEENDFLPDLDA
VDEATWEKARCIFVNYPNNPTAAMAPRSFFEKLIGIARKHNVIVVHDAAYTEMYYNENNRPLSIMEIPGAMDVAIEFNSL
SKPYNMTGWRIAMAVGNASLVAGLGKVKENMDSGAFQAVQEAAIVALRDGDAFLAEIRDIYRKRRDTVIAALNKIGITCR
VPEASLYVWARVPEGYTSSDFVTRVLQETGVVMTPGNGFGAAGEGYFRISLTVNDERLEEAVSRIASL
>Mature_387_residues
TQFTLADRLATLPPYLFAQIDKVKAEVAARGVDIISLGIGDPDMPTPDFVIEALKKAAEKPANHQYPSYTGMLAFRQEVA
NWYKRRYAVELDPKTEVLTLIGSKEGIAHFPTAFVNPGDLVLVCPPCYPVYAIASRFMGGVVQELPLLEENDFLPDLDAV
DEATWEKARCIFVNYPNNPTAAMAPRSFFEKLIGIARKHNVIVVHDAAYTEMYYNENNRPLSIMEIPGAMDVAIEFNSLS
KPYNMTGWRIAMAVGNASLVAGLGKVKENMDSGAFQAVQEAAIVALRDGDAFLAEIRDIYRKRRDTVIAALNKIGITCRV
PEASLYVWARVPEGYTSSDFVTRVLQETGVVMTPGNGFGAAGEGYFRISLTVNDERLEEAVSRIASL

Specific function: Involved in the synthesis of meso-diaminopimelate (m-DAP or DL-DAP), required for both lysine and peptidoglycan biosynthesis. Catalyzes the direct conversion of tetrahydrodipicolinate to LL-diaminopimelate, a reaction that requires three enzymes in E.coli

COG id: COG0436

COG function: function code E; Aspartate/tyrosine/aromatic aminotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. LL-diaminopimelate aminotransferase subfamily

Homologues:

Organism=Homo sapiens, GI56713256, Length=346, Percent_Identity=23.121387283237, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI56713254, Length=346, Percent_Identity=23.121387283237, Blast_Score=112, Evalue=7e-25,
Organism=Homo sapiens, GI95147551, Length=340, Percent_Identity=22.6470588235294, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI169881279, Length=340, Percent_Identity=22.6470588235294, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI4507369, Length=372, Percent_Identity=26.8817204301075, Blast_Score=102, Evalue=6e-22,
Organism=Homo sapiens, GI169881281, Length=329, Percent_Identity=20.9726443768997, Blast_Score=79, Evalue=5e-15,
Organism=Escherichia coli, GI1788722, Length=389, Percent_Identity=39.3316195372751, Blast_Score=310, Evalue=1e-85,
Organism=Escherichia coli, GI1786816, Length=384, Percent_Identity=26.0416666666667, Blast_Score=124, Evalue=7e-30,
Organism=Escherichia coli, GI1788627, Length=374, Percent_Identity=25.1336898395722, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI1788332, Length=289, Percent_Identity=27.681660899654, Blast_Score=76, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI71994472, Length=413, Percent_Identity=28.3292978208232, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI71994476, Length=420, Percent_Identity=28.0952380952381, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17567369, Length=316, Percent_Identity=24.6835443037975, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17567663, Length=369, Percent_Identity=23.5772357723577, Blast_Score=89, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6322401, Length=391, Percent_Identity=24.2966751918159, Blast_Score=112, Evalue=1e-25,
Organism=Drosophila melanogaster, GI28573069, Length=421, Percent_Identity=23.0403800475059, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24646114, Length=421, Percent_Identity=23.0403800475059, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI28573067, Length=421, Percent_Identity=23.0403800475059, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI28573065, Length=421, Percent_Identity=23.0403800475059, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI18859735, Length=372, Percent_Identity=23.1182795698925, Blast_Score=79, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPAT_DESDG (Q30ZX9)

Other databases:

- EMBL:   CP000112
- RefSeq:   YP_388462.1
- ProteinModelPortal:   Q30ZX9
- SMR:   Q30ZX9
- STRING:   Q30ZX9
- GeneID:   3756976
- GenomeReviews:   CP000112_GR
- KEGG:   dde:Dde_1970
- NMPDR:   fig|207559.3.peg.737
- eggNOG:   COG0436
- HOGENOM:   HBG645860
- ProtClustDB:   PRK09276
- BioCyc:   DDES207559:DDE_1970-MONOMER
- HAMAP:   MF_01642
- InterPro:   IPR004839
- InterPro:   IPR019881
- InterPro:   IPR004838
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- TIGRFAMs:   TIGR03540

Pfam domain/function: PF00155 Aminotran_1_2; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.6.1.83

Molecular weight: Translated: 42773; Mature: 42642

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS00105 AA_TRANSFER_CLASS_1

Important sites: BINDING 41-41 BINDING 67-67 BINDING 129-129 BINDING 179-179 BINDING 179-179 BINDING 207-207 BINDING 210-210 BINDING 239-239 BINDING 241-241 BINDING 250-250 BINDING 368-368

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQFTLADRLATLPPYLFAQIDKVKAEVAARGVDIISLGIGDPDMPTPDFVIEALKKAAE
CCCCCHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHC
KPANHQYPSYTGMLAFRQEVANWYKRRYAVELDPKTEVLTLIGSKEGIAHFPTAFVNPGD
CCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEECCCCEEEEEECCCCCCCCCCCCCCCCCC
LVLVCPPCYPVYAIASRFMGGVVQELPLLEENDFLPDLDAVDEATWEKARCIFVNYPNNP
EEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCEEEEEECCCCC
TAAMAPRSFFEKLIGIARKHNVIVVHDAAYTEMYYNENNRPLSIMEIPGAMDVAIEFNSL
CHHCCCHHHHHHHHHHHHHCCEEEEECCCEEEEEECCCCCEEEEEECCCCEEEEEEECCC
SKPYNMTGWRIAMAVGNASLVAGLGKVKENMDSGAFQAVQEAAIVALRDGDAFLAEIRDI
CCCCCCCCEEEEEEECCHHHHHHHHHHHHCCCHHHHHHHHHHEEEEEECCCHHHHHHHHH
YRKRRDTVIAALNKIGITCRVPEASLYVWARVPEGYTSSDFVTRVLQETGVVMTPGNGFG
HHHHHHHHHHHHHHCCCEEECCCCCEEEEEECCCCCCCHHHHHHHHHHCCEEEECCCCCC
AAGEGYFRISLTVNDERLEEAVSRIASL
CCCCCEEEEEEEECHHHHHHHHHHHHCC
>Mature Secondary Structure 
TQFTLADRLATLPPYLFAQIDKVKAEVAARGVDIISLGIGDPDMPTPDFVIEALKKAAE
CCCCHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHC
KPANHQYPSYTGMLAFRQEVANWYKRRYAVELDPKTEVLTLIGSKEGIAHFPTAFVNPGD
CCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEECCCCEEEEEECCCCCCCCCCCCCCCCCC
LVLVCPPCYPVYAIASRFMGGVVQELPLLEENDFLPDLDAVDEATWEKARCIFVNYPNNP
EEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCEEEEEECCCCC
TAAMAPRSFFEKLIGIARKHNVIVVHDAAYTEMYYNENNRPLSIMEIPGAMDVAIEFNSL
CHHCCCHHHHHHHHHHHHHCCEEEEECCCEEEEEECCCCCEEEEEECCCCEEEEEEECCC
SKPYNMTGWRIAMAVGNASLVAGLGKVKENMDSGAFQAVQEAAIVALRDGDAFLAEIRDI
CCCCCCCCEEEEEEECCHHHHHHHHHHHHCCCHHHHHHHHHHEEEEEECCCHHHHHHHHH
YRKRRDTVIAALNKIGITCRVPEASLYVWARVPEGYTSSDFVTRVLQETGVVMTPGNGFG
HHHHHHHHHHHHHHCCCEEECCCCCEEEEEECCCCCCCHHHHHHHHHHCCEEEECCCCCC
AAGEGYFRISLTVNDERLEEAVSRIASL
CCCCCEEEEEEEECHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA