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The map label for this gene is dapF

Identifier: 78356841

GI number: 78356841

Start: 1848213

End: 1849064

Strand: Reverse

Name: dapF

Synonym: Dde_1798

Alternate gene names: 78356841

Gene position: 1849064-1848213 (Counterclockwise)

Preceding gene: 78356842

Following gene: 78356840

Centisome position: 49.57

GC content: 56.1

Gene sequence:

>852_bases
ATGACGCGGACACCGCGTGCATCCGTGCCTTTTTACAAGATGCAGGGATGTGGAAACGATTTCGTATTCATTGACAACAG
AGAGCTGAAGCTGCCTGTTGCCCTTATGGAAGACTGGGCGCGCAAAATCTGCAGGCGTTCTTTTTCCGTCGGTGCGGACG
GCCTTGTTTTTCTGGAAGAAGCTCCGGACATTCCCGGTGTTGACTACCGGTGGCATTTTTATAATGCCGATGGATCCCGT
GCTGAAATGTGCGGCAACGCTTCACGTTGTGCCGCGCTTCTTGCTTTCCGTACCGGCTTTGCCGGTGCCGAACATGTGCT
GGGAACCGATGCGGGACCGGTACGTGCCAGAGTGGATGTTGCCGCAGGCATGGTTAAAGTGGAACTGACTCCGCCAAAAG
AGCTTAGTCTTAACAATGTGCTGCGTTTTGGCGATGAGACACACGAAATTCATTTCGTCAATACAGGGGTGCCGCATGCC
GTCTGGCTGGACGCAGATGCAGCGGCACTGGACGTACGGGCGCTGGGAGCCTCTTTGCGCTATCATGAGCGTTTCGCACC
TGCCGGCACCAATGCTAACTTTGTCACCGTGATTGATCAGCACAACATTTTGCTGCGCACCTATGAGCGCGGCGTGGAAG
ACGAAACCTATGCATGCGGTACCGGCGCGGCCGCAGGGGCTGTTATCACCCATGCGTTGGGGCTTACCGGCCCGCAGGTG
ACAGTGCGCAGTTCCGGCGGCGAACTGCTGGGCATATCGCTGGAAGACGGCTCGGTGTTTCTTGAAGGAAAGGCCCTGCT
CGTCTACACAGGCGAAATGCAGCCCGAGTCACTGGGTATTTCACTAGATTAG

Upstream 100 bases:

>100_bases
GCATTGTGGTGCGTGAAGTCTGCCGCCGGAGAGGTACCGCGACAGACCGAATGCTCTGGTGATTGCGGGCACATAACGGC
AACCTCAGGAGAATGATCTC

Downstream 100 bases:

>100_bases
TTTCAACGGCCGGTTTTTCCGGCCATTTGCTTTTGCAAACACAGGAGAAGCGTATGCAGTTTTCCGGAGCACTGACCGCC
ATCGTCACCCCCCTCAAAAA

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase [H]

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MTRTPRASVPFYKMQGCGNDFVFIDNRELKLPVALMEDWARKICRRSFSVGADGLVFLEEAPDIPGVDYRWHFYNADGSR
AEMCGNASRCAALLAFRTGFAGAEHVLGTDAGPVRARVDVAAGMVKVELTPPKELSLNNVLRFGDETHEIHFVNTGVPHA
VWLDADAAALDVRALGASLRYHERFAPAGTNANFVTVIDQHNILLRTYERGVEDETYACGTGAAAGAVITHALGLTGPQV
TVRSSGGELLGISLEDGSVFLEGKALLVYTGEMQPESLGISLD

Sequences:

>Translated_283_residues
MTRTPRASVPFYKMQGCGNDFVFIDNRELKLPVALMEDWARKICRRSFSVGADGLVFLEEAPDIPGVDYRWHFYNADGSR
AEMCGNASRCAALLAFRTGFAGAEHVLGTDAGPVRARVDVAAGMVKVELTPPKELSLNNVLRFGDETHEIHFVNTGVPHA
VWLDADAAALDVRALGASLRYHERFAPAGTNANFVTVIDQHNILLRTYERGVEDETYACGTGAAAGAVITHALGLTGPQV
TVRSSGGELLGISLEDGSVFLEGKALLVYTGEMQPESLGISLD
>Mature_282_residues
TRTPRASVPFYKMQGCGNDFVFIDNRELKLPVALMEDWARKICRRSFSVGADGLVFLEEAPDIPGVDYRWHFYNADGSRA
EMCGNASRCAALLAFRTGFAGAEHVLGTDAGPVRARVDVAAGMVKVELTPPKELSLNNVLRFGDETHEIHFVNTGVPHAV
WLDADAAALDVRALGASLRYHERFAPAGTNANFVTVIDQHNILLRTYERGVEDETYACGTGAAAGAVITHALGLTGPQVT
VRSSGGELLGISLEDGSVFLEGKALLVYTGEMQPESLGISLD

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family [H]

Homologues:

Organism=Escherichia coli, GI87082334, Length=273, Percent_Identity=30.4029304029304, Blast_Score=111, Evalue=4e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001653
- InterPro:   IPR018510 [H]

Pfam domain/function: PF01678 DAP_epimerase [H]

EC number: =5.1.1.7 [H]

Molecular weight: Translated: 30484; Mature: 30353

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRTPRASVPFYKMQGCGNDFVFIDNRELKLPVALMEDWARKICRRSFSVGADGLVFLEE
CCCCCCCCCCEEEECCCCCCEEEEECCCEEEHHHHHHHHHHHHHHHHCCCCCCCEEEEEC
APDIPGVDYRWHFYNADGSRAEMCGNASRCAALLAFRTGFAGAEHVLGTDAGPVRARVDV
CCCCCCCCEEEEEECCCCCHHHHCCCHHHHHHHHHHHCCCCCCCHHCCCCCCCCEEEEEE
AAGMVKVELTPPKELSLNNVLRFGDETHEIHFVNTGVPHAVWLDADAAALDVRALGASLR
ECCEEEEEECCCCCCCHHHHHHCCCCCEEEEEEECCCCEEEEECCCCCEEEHHHHCCHHH
YHERFAPAGTNANFVTVIDQHNILLRTYERGVEDETYACGTGAAAGAVITHALGLTGPQV
HHHHCCCCCCCCCEEEEEECCCEEEEEHHCCCCCCCEECCCCCHHHHHHHHHHCCCCCEE
TVRSSGGELLGISLEDGSVFLEGKALLVYTGEMQPESLGISLD
EEECCCCCEEEEEECCCCEEEECCEEEEEECCCCCHHCCCCCC
>Mature Secondary Structure 
TRTPRASVPFYKMQGCGNDFVFIDNRELKLPVALMEDWARKICRRSFSVGADGLVFLEE
CCCCCCCCCEEEECCCCCCEEEEECCCEEEHHHHHHHHHHHHHHHHCCCCCCCEEEEEC
APDIPGVDYRWHFYNADGSRAEMCGNASRCAALLAFRTGFAGAEHVLGTDAGPVRARVDV
CCCCCCCCEEEEEECCCCCHHHHCCCHHHHHHHHHHHCCCCCCCHHCCCCCCCCEEEEEE
AAGMVKVELTPPKELSLNNVLRFGDETHEIHFVNTGVPHAVWLDADAAALDVRALGASLR
ECCEEEEEECCCCCCCHHHHHHCCCCCEEEEEEECCCCEEEEECCCCCEEEHHHHCCHHH
YHERFAPAGTNANFVTVIDQHNILLRTYERGVEDETYACGTGAAAGAVITHALGLTGPQV
HHHHCCCCCCCCCEEEEEECCCEEEEEHHCCCCCCCEECCCCCHHHHHHHHHHCCCCCEE
TVRSSGGELLGISLEDGSVFLEGKALLVYTGEMQPESLGISLD
EEECCCCCEEEEEECCCCEEEECCEEEEEECCCCCHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]