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The map label for this gene is gcvH

Identifier: 78356736

GI number: 78356736

Start: 1746113

End: 1746496

Strand: Reverse

Name: gcvH

Synonym: Dde_1693

Alternate gene names: 78356736

Gene position: 1746496-1746113 (Counterclockwise)

Preceding gene: 78356737

Following gene: 78356735

Centisome position: 46.82

GC content: 54.69

Gene sequence:

>384_bases
ATGAATATTCCCGCAGAACTGAAGTTCTCGAAAAGTCATGAATGGGTCCGCCTTGAAGGCGATGAGGCAGTGATCGGCAT
AACCGAGTTTGCTCAGGAACAGCTGGGCGATCTTACTTTCATTGAACTGCCCGCCGCAGGCGATACCCTGACAGCAGGCG
AAGAAATGGGGTCCGTCGAGTCCGTCAAAGCCGCAAGTGAACTGTACGCACCTGTCTCCGGCGAAGTGACAGAGGTGAAC
CTGCACCTTGAAGATGCTCCCGAAGCCGTAAACGAATCGCCCTACGACAAAGGCTGGATGCTGAAGGTCAAAGTGTCTGC
CGTGGCTGGCAGTCTGCTTTCCGCAGAAGAATACGCAGCCCTCATCGAAAGCGAAGCGCACTAG

Upstream 100 bases:

>100_bases
CATAAGCGCCATGGCCGTTTTCATTTTATTTGCCTTGCCCCGCACGCGTTGTGGATGTAAAAAGCGAACCAACCACACCA
ACTTTTTCAGGAGAAGAATC

Downstream 100 bases:

>100_bases
GCCGCAAGGAGAACTTTCATGCCCTACACTCCGCACACTCCCGAAGAAATCCGCCAGATGCTGGATGTCATCGGGGTGAA
AAGCATTGATGACCTGTTTG

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 127; Mature: 127

Protein sequence:

>127_residues
MNIPAELKFSKSHEWVRLEGDEAVIGITEFAQEQLGDLTFIELPAAGDTLTAGEEMGSVESVKAASELYAPVSGEVTEVN
LHLEDAPEAVNESPYDKGWMLKVKVSAVAGSLLSAEEYAALIESEAH

Sequences:

>Translated_127_residues
MNIPAELKFSKSHEWVRLEGDEAVIGITEFAQEQLGDLTFIELPAAGDTLTAGEEMGSVESVKAASELYAPVSGEVTEVN
LHLEDAPEAVNESPYDKGWMLKVKVSAVAGSLLSAEEYAALIESEAH
>Mature_127_residues
MNIPAELKFSKSHEWVRLEGDEAVIGITEFAQEQLGDLTFIELPAAGDTLTAGEEMGSVESVKAASELYAPVSGEVTEVN
LHLEDAPEAVNESPYDKGWMLKVKVSAVAGSLLSAEEYAALIESEAH

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Homo sapiens, GI49574537, Length=119, Percent_Identity=47.0588235294118, Blast_Score=109, Evalue=5e-25,
Organism=Homo sapiens, GI89057342, Length=119, Percent_Identity=46.218487394958, Blast_Score=106, Evalue=4e-24,
Organism=Escherichia coli, GI1789271, Length=127, Percent_Identity=54.3307086614173, Blast_Score=133, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI17507493, Length=119, Percent_Identity=47.8991596638655, Blast_Score=119, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17551294, Length=112, Percent_Identity=47.3214285714286, Blast_Score=115, Evalue=8e-27,
Organism=Saccharomyces cerevisiae, GI6319272, Length=122, Percent_Identity=43.4426229508197, Blast_Score=108, Evalue=3e-25,
Organism=Drosophila melanogaster, GI17865652, Length=122, Percent_Identity=45.0819672131148, Blast_Score=109, Evalue=4e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GCSH_DESDG (Q311A6)

Other databases:

- EMBL:   CP000112
- RefSeq:   YP_388185.1
- ProteinModelPortal:   Q311A6
- SMR:   Q311A6
- STRING:   Q311A6
- GeneID:   3756691
- GenomeReviews:   CP000112_GR
- KEGG:   dde:Dde_1693
- NMPDR:   fig|207559.3.peg.1486
- eggNOG:   COG0509
- HOGENOM:   HBG693789
- OMA:   TSDHEWL
- ProtClustDB:   PRK01202
- BioCyc:   DDES207559:DDE_1693-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_00272
- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053
- PANTHER:   PTHR11715
- TIGRFAMs:   TIGR00527

Pfam domain/function: PF01597 GCV_H; SSF51230 Hybrid_motif

EC number: NA

Molecular weight: Translated: 13606; Mature: 13606

Theoretical pI: Translated: 3.91; Mature: 3.91

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIPAELKFSKSHEWVRLEGDEAVIGITEFAQEQLGDLTFIELPAAGDTLTAGEEMGSVE
CCCCCCEEECCCCCEEEEECCCEEEEHHHHHHHHHCCEEEEEECCCCCCCCCCHHCCCHH
SVKAASELYAPVSGEVTEVNLHLEDAPEAVNESPYDKGWMLKVKVSAVAGSLLSAEEYAA
HHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCCCCEEEEEEHHHHHCCHHCHHHHHH
LIESEAH
HHHCCCC
>Mature Secondary Structure
MNIPAELKFSKSHEWVRLEGDEAVIGITEFAQEQLGDLTFIELPAAGDTLTAGEEMGSVE
CCCCCCEEECCCCCEEEEECCCEEEEHHHHHHHHHCCEEEEEECCCCCCCCCCHHCCCHH
SVKAASELYAPVSGEVTEVNLHLEDAPEAVNESPYDKGWMLKVKVSAVAGSLLSAEEYAA
HHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCCCCEEEEEEHHHHHCCHHCHHHHHH
LIESEAH
HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA