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The map label for this gene is pcaR [H]

Identifier: 78356589

GI number: 78356589

Start: 1564706

End: 1565488

Strand: Reverse

Name: pcaR [H]

Synonym: Dde_1546

Alternate gene names: 78356589

Gene position: 1565488-1564706 (Counterclockwise)

Preceding gene: 78356607

Following gene: 78356580

Centisome position: 41.97

GC content: 54.66

Gene sequence:

>783_bases
ATGGCAGAAGAACCGGCCGTTACTGACAGACGCCCTGACCCGCTTTTTGTACATTCACTTCAACGGGCTCTGATGGTTCT
GGAGGCCTTCGATTCCACTGCCCGCAATCTGAGTCTGTCGGACCTGAGCCGCAAGACAGGGCTGAACAAAAGCGCTGTTC
AGCGCTTTCTCTTTACATGGGAGGCCCTTGGCTATCTGGAAAAAGACACAGATACCAAGCGTTATTCTTTAAGTCCAAAG
GTAATGTCGCTCAGCTATCATTATTTGAAGGACAAACGACTTGTGGAAATTGCCACACCGGTGCTGCTGGAAATGCGTAA
AAGAACAGGATGCGCCAGTTATCTGGGTATTCTGTACGAGCAAGACGTTATGTATCTTATACGCCTGCCCCAGCGGTTCA
TATTGCTTGAAAGCACGCTGGCGGGCAGAAGGGTGCCCGCATTCTGCGGCGGCAGGGCTCTGCTTTCGTGTCTGCCGCAA
AACAGCGCCCGCAGCCTGATAGAACAGAGCATCAGAACGCAGCTGACGCCGTTCACCATCACTGACAGCGAGGCAAACAT
GCGGGCCGTACGGCAGGCGGCAGAACTGGGGTATGCGGTTTCGCGTCAGGAGCAGCTCATAGGTGAAGTTTCCGTTTCAG
CACCCGTACTATCGTCCGGCGGTACACTGCTGGCCGCTGTCTATATTTCTGCAGACAACAACGAATGGGACGACAGCCGC
ATTGCCGCCGAGCTGGCACCGGTGGTACTCAAAGCCGCATCAGACATCGGCCGCCAGTTCTGA

Upstream 100 bases:

>100_bases
TGTCAACAACAACATCAATAACGACAGTTGTGCAACCAAACAACATCATACTTCCACGCAGCACACGACAAACACAAAGC
AGCACAGCAGGGAGGCCTTC

Downstream 100 bases:

>100_bases
TGGCACGCCGCGTGCCGGTGGAAAGCTGCATAAATAGTGCGGTGCACCCGATCCCGCTGCATACCGGAGAATCATACAGA
ATACAAAAGCCCCGCAGGCT

Product: IclR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MAEEPAVTDRRPDPLFVHSLQRALMVLEAFDSTARNLSLSDLSRKTGLNKSAVQRFLFTWEALGYLEKDTDTKRYSLSPK
VMSLSYHYLKDKRLVEIATPVLLEMRKRTGCASYLGILYEQDVMYLIRLPQRFILLESTLAGRRVPAFCGGRALLSCLPQ
NSARSLIEQSIRTQLTPFTITDSEANMRAVRQAAELGYAVSRQEQLIGEVSVSAPVLSSGGTLLAAVYISADNNEWDDSR
IAAELAPVVLKAASDIGRQF

Sequences:

>Translated_260_residues
MAEEPAVTDRRPDPLFVHSLQRALMVLEAFDSTARNLSLSDLSRKTGLNKSAVQRFLFTWEALGYLEKDTDTKRYSLSPK
VMSLSYHYLKDKRLVEIATPVLLEMRKRTGCASYLGILYEQDVMYLIRLPQRFILLESTLAGRRVPAFCGGRALLSCLPQ
NSARSLIEQSIRTQLTPFTITDSEANMRAVRQAAELGYAVSRQEQLIGEVSVSAPVLSSGGTLLAAVYISADNNEWDDSR
IAAELAPVVLKAASDIGRQF
>Mature_259_residues
AEEPAVTDRRPDPLFVHSLQRALMVLEAFDSTARNLSLSDLSRKTGLNKSAVQRFLFTWEALGYLEKDTDTKRYSLSPKV
MSLSYHYLKDKRLVEIATPVLLEMRKRTGCASYLGILYEQDVMYLIRLPQRFILLESTLAGRRVPAFCGGRALLSCLPQN
SARSLIEQSIRTQLTPFTITDSEANMRAVRQAAELGYAVSRQEQLIGEVSVSAPVLSSGGTLLAAVYISADNNEWDDSRI
AAELAPVVLKAASDIGRQF

Specific function: Positive regulator of all genes within the pca regulon, pcaBDC, pcaIJ and pcaF. Also required for the chemotactic response to aromatic compounds [H]

COG id: COG1414

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 iclR-ED (iclR effector binding) domain [H]

Homologues:

Organism=Escherichia coli, GI1786468, Length=229, Percent_Identity=28.82096069869, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1788131, Length=254, Percent_Identity=27.1653543307087, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1786715, Length=241, Percent_Identity=23.2365145228216, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1789999, Length=234, Percent_Identity=25.2136752136752, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI87082359, Length=257, Percent_Identity=22.1789883268482, Blast_Score=64, Evalue=7e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012794
- InterPro:   IPR014757
- InterPro:   IPR005471
- InterPro:   IPR011991 [H]

Pfam domain/function: PF09339 HTH_IclR; PF01614 IclR [H]

EC number: NA

Molecular weight: Translated: 28922; Mature: 28790

Theoretical pI: Translated: 8.43; Mature: 8.43

Prosite motif: PS51077 HTH_ICLR ; PS51078 ICLR_ED

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEEPAVTDRRPDPLFVHSLQRALMVLEAFDSTARNLSLSDLSRKTGLNKSAVQRFLFTW
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHH
EALGYLEKDTDTKRYSLSPKVMSLSYHYLKDKRLVEIATPVLLEMRKRTGCASYLGILYE
HHHHHHHCCCCCCEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHH
QDVMYLIRLPQRFILLESTLAGRRVPAFCGGRALLSCLPQNSARSLIEQSIRTQLTPFTI
HHHHHHHHCCHHHHHHHHHHCCCCCCHHHCHHHHHHHCCCHHHHHHHHHHHHHCCCCEEE
TDSEANMRAVRQAAELGYAVSRQEQLIGEVSVSAPVLSSGGTLLAAVYISADNNEWDDSR
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEECCCCCCCHHH
IAAELAPVVLKAASDIGRQF
HHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AEEPAVTDRRPDPLFVHSLQRALMVLEAFDSTARNLSLSDLSRKTGLNKSAVQRFLFTW
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHH
EALGYLEKDTDTKRYSLSPKVMSLSYHYLKDKRLVEIATPVLLEMRKRTGCASYLGILYE
HHHHHHHCCCCCCEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHH
QDVMYLIRLPQRFILLESTLAGRRVPAFCGGRALLSCLPQNSARSLIEQSIRTQLTPFTI
HHHHHHHHCCHHHHHHHHHHCCCCCCHHHCHHHHHHHCCCHHHHHHHHHHHHHCCCCEEE
TDSEANMRAVRQAAELGYAVSRQEQLIGEVSVSAPVLSSGGTLLAAVYISADNNEWDDSR
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEECCCCCCCHHH
IAAELAPVVLKAASDIGRQF
HHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8083169 [H]