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The map label for this gene is punA [H]

Identifier: 78356580

GI number: 78356580

Start: 1556251

End: 1557081

Strand: Reverse

Name: punA [H]

Synonym: Dde_1537

Alternate gene names: 78356580

Gene position: 1557081-1556251 (Counterclockwise)

Preceding gene: 78356589

Following gene: 78356578

Centisome position: 41.74

GC content: 59.21

Gene sequence:

>831_bases
ATGCAAAACCCGAAAAAAGTACAGAACGCTTCGGAATTTCTGAAAAAAAAGTTATCAGGGCACCCCGACCCCAAAGTTGG
AATCGTTCTGGGCACCGGCCTTGGCGGACTGGTTGATGCCGTCAGCATTCATACCGTGATTGATTACGGTGAGATTCCCG
ATTTTCCGCGTTCCACAGTCGCTTCGCATCAGGGCAGATTCATAGCCGGCAGCATCGGCAGCACCCCCGTGCTGCTCCAG
CAGGGCCGCTGCCACCTGTATGAAGGCTATTCAGCAGGTGACGTATGCACCGGTGTGCGCACCATGGCCGCATGCGGAGC
TGACACCCTGATAATCACCAACGCGGCAGGCGCTCTGAATCCCGCATGGTCCGCGGGTGATCTCATGGCCATAACCGACC
ACATAAATTTTACGGGACAAAGCCCGCTGACCGGCCCGAACAATGACCTGTGGGGACCGCGCTTTCCTGACATGAGTGCC
CCCTACGACGCACAACTTATCTGTGCAGCCATGCAAAAGGCTTCGGAGCTGGGCATCCGTCTGGAACGCGGCGTCTACGC
CGGAGTGCGCGGCCCGCAGATGGAAACCCCCGCGGAGACCCGCATGTACAAAGCGGCAGGAGCGGATGCCGTGGGCATGT
CCACAGTGCTCGAAGTTATCGCCGCCCGCCACATGGGTCTGAAAGTGCTGGGCGTTTCATGCCTGACCAACAAAAACCTG
CCGGACTGCATGCAGGAGGCGCCTCTGGAGGAAGTCATCCGCGTGGCCAGTCTTGCCGGAAACAGGCTGGCAAAGCTCAT
TGCCGCGGTTACCGCCGACCTGCCTGCCTGA

Upstream 100 bases:

>100_bases
GGAAGACCGGCCATGAAGGCGCAGGCCATCCATGAACCGATCGCAGAAGTATGATCGCGTCCCTTTTCTACACTCAATCA
ATACAATATTCTGGAGAGTT

Downstream 100 bases:

>100_bases
CAGACACGCGTTTTCTGAAAAACCGAAGCTCCGGCACCGGTGCATGTGCACCGGTGCCGGAGCATTTTTTGTCAGCAAAA
ATGCGGGCAGACAACAGCCC

Product: purine nucleoside phosphorylase

Products: NA

Alternate protein names: Inosine phosphorylase; Purine nucleoside phosphorylase I; PNP I; PU-NPase I [H]

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MQNPKKVQNASEFLKKKLSGHPDPKVGIVLGTGLGGLVDAVSIHTVIDYGEIPDFPRSTVASHQGRFIAGSIGSTPVLLQ
QGRCHLYEGYSAGDVCTGVRTMAACGADTLIITNAAGALNPAWSAGDLMAITDHINFTGQSPLTGPNNDLWGPRFPDMSA
PYDAQLICAAMQKASELGIRLERGVYAGVRGPQMETPAETRMYKAAGADAVGMSTVLEVIAARHMGLKVLGVSCLTNKNL
PDCMQEAPLEEVIRVASLAGNRLAKLIAAVTADLPA

Sequences:

>Translated_276_residues
MQNPKKVQNASEFLKKKLSGHPDPKVGIVLGTGLGGLVDAVSIHTVIDYGEIPDFPRSTVASHQGRFIAGSIGSTPVLLQ
QGRCHLYEGYSAGDVCTGVRTMAACGADTLIITNAAGALNPAWSAGDLMAITDHINFTGQSPLTGPNNDLWGPRFPDMSA
PYDAQLICAAMQKASELGIRLERGVYAGVRGPQMETPAETRMYKAAGADAVGMSTVLEVIAARHMGLKVLGVSCLTNKNL
PDCMQEAPLEEVIRVASLAGNRLAKLIAAVTADLPA
>Mature_276_residues
MQNPKKVQNASEFLKKKLSGHPDPKVGIVLGTGLGGLVDAVSIHTVIDYGEIPDFPRSTVASHQGRFIAGSIGSTPVLLQ
QGRCHLYEGYSAGDVCTGVRTMAACGADTLIITNAAGALNPAWSAGDLMAITDHINFTGQSPLTGPNNDLWGPRFPDMSA
PYDAQLICAAMQKASELGIRLERGVYAGVRGPQMETPAETRMYKAAGADAVGMSTVLEVIAARHMGLKVLGVSCLTNKNL
PDCMQEAPLEEVIRVASLAGNRLAKLIAAVTADLPA

Specific function: Cleavage of guanosine or inosine to respective bases and sugar-1-phosphate molecules [H]

COG id: COG0005

COG function: function code F; Purine nucleoside phosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP/MTAP phosphorylase family [H]

Homologues:

Organism=Homo sapiens, GI157168362, Length=275, Percent_Identity=44.3636363636364, Blast_Score=232, Evalue=2e-61,
Organism=Escherichia coli, GI1788746, Length=218, Percent_Identity=42.6605504587156, Blast_Score=176, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI17541190, Length=255, Percent_Identity=40.7843137254902, Blast_Score=174, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6323238, Length=282, Percent_Identity=40.4255319148936, Blast_Score=179, Evalue=4e-46,
Organism=Drosophila melanogaster, GI24656090, Length=260, Percent_Identity=41.1538461538462, Blast_Score=202, Evalue=3e-52,
Organism=Drosophila melanogaster, GI24656093, Length=260, Percent_Identity=41.1538461538462, Blast_Score=201, Evalue=3e-52,
Organism=Drosophila melanogaster, GI45552887, Length=260, Percent_Identity=41.1538461538462, Blast_Score=201, Evalue=4e-52,
Organism=Drosophila melanogaster, GI45552885, Length=260, Percent_Identity=41.1538461538462, Blast_Score=201, Evalue=5e-52,
Organism=Drosophila melanogaster, GI24762376, Length=223, Percent_Identity=35.8744394618834, Blast_Score=133, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011268
- InterPro:   IPR000845
- InterPro:   IPR011270
- InterPro:   IPR001369
- InterPro:   IPR018099 [H]

Pfam domain/function: PF01048 PNP_UDP_1 [H]

EC number: =2.4.2.1 [H]

Molecular weight: Translated: 28937; Mature: 28937

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNPKKVQNASEFLKKKLSGHPDPKVGIVLGTGLGGLVDAVSIHTVIDYGEIPDFPRSTV
CCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHEECCCCCCCCCHHH
ASHQGRFIAGSIGSTPVLLQQGRCHLYEGYSAGDVCTGVRTMAACGADTLIITNAAGALN
HHCCCCEEECCCCCCCEEEECCCEEEECCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCC
PAWSAGDLMAITDHINFTGQSPLTGPNNDLWGPRFPDMSAPYDAQLICAAMQKASELGIR
CCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCEE
LERGVYAGVRGPQMETPAETRMYKAAGADAVGMSTVLEVIAARHMGLKVLGVSCLTNKNL
EECCEEECCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCEEEEEEHHHCCCCC
PDCMQEAPLEEVIRVASLAGNRLAKLIAAVTADLPA
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MQNPKKVQNASEFLKKKLSGHPDPKVGIVLGTGLGGLVDAVSIHTVIDYGEIPDFPRSTV
CCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHEECCCCCCCCCHHH
ASHQGRFIAGSIGSTPVLLQQGRCHLYEGYSAGDVCTGVRTMAACGADTLIITNAAGALN
HHCCCCEEECCCCCCCEEEECCCEEEECCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCC
PAWSAGDLMAITDHINFTGQSPLTGPNNDLWGPRFPDMSAPYDAQLICAAMQKASELGIR
CCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCEE
LERGVYAGVRGPQMETPAETRMYKAAGADAVGMSTVLEVIAARHMGLKVLGVSCLTNKNL
EECCEEECCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCEEEEEEHHHCCCCC
PDCMQEAPLEEVIRVASLAGNRLAKLIAAVTADLPA
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10537218; 8969508; 9384377; 1629150 [H]