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The map label for this gene is priA [H]

Identifier: 78356564

GI number: 78356564

Start: 1538932

End: 1541403

Strand: Direct

Name: priA [H]

Synonym: Dde_1519

Alternate gene names: 78356564

Gene position: 1538932-1541403 (Clockwise)

Preceding gene: 78356563

Following gene: 78356565

Centisome position: 41.26

GC content: 60.36

Gene sequence:

>2472_bases
ATGATTCTGTGCGGGCGGCGGTGTCGCCCGCTTTTTTTCGTCACTTCCGGCAACCGGCAACTCGTTATGAATGAAAATCT
GCGTATAGTTTCAGTGTCGCTTATGGCTCCGCCGTATACCACGCTGCATTACAGGCTGCCGGAGTGGCTGCCCGAAGAAA
TGTGGTGCGCGGGGTTGCGGGTGGCTGTGCCGCTGGGGGCGCGCGGTACCCTGCGTGCGGGGGTTATCGCTGCATGCGGT
TGCGCTCCGGACACCGTGCCGCAGGGCGTTGAGCTGAAGGCGGTACTCTGGCCGCTGGAGTTTGTTCCTCTGCTGGGCGA
AGAGTATCTGGAAATGGTCCGCCAGCTTGCCGTGCGCCAGATGGTGCCCCCCGGACAGATTCTGGGCATGCTGCTGCCTG
CAGGGCTGCGCAGCGCCGGTGTGCGTCTGCGCGTGCGTGCCGGAGGCAGACCCCGTACGCTGCGGCTGCGCGATATTGCA
AAAATGGCTGACTCCGAGCGCCATGTGCTGGCCTCGCACTGGAGTGCGGGCAGGGTGGAAGTGCTGGAACCGGAAACTGA
CGAGGCGGAGAATGACATATGCCATGTGACTGCAGATCCGCCGTGGCCGGTACGTCCGGCGGCCAGACGTCAGACAGAAG
TGCTCGAATATCTGTGGGAGAACGGACCTGTTTCGCGGCGACGCATGCTGCATGATTTGGGTACAGGTGCACAGACGCCT
CTGCTGACGCTGGCGGAACGGGGTCTTGTGCATGTGGGGCCGCTTGATGCAGAGCAGGCGGAAGTTTGCGGATGCGGTAT
TGACAGCGCGTGTCACACTGAGGCGTTTGAGAATCAGCTTTTTGAGCTGACTGATGCGCAGCAGGCGGCGTTGCGGGCCT
GCACTGACGCTGTGGAGTCAGACAGCGCGGAAACCCGTCTGCTCTTCGGCATCACCGGCAGCGGCAAAACCGCCGTGTAT
CTGGAACTGGCCCGGGCCTGTCTGGAAAAAGGGCTGTCCGTCATGCTGCTTGCGCCTGAAGTGGCGCTGGCCTGTAAATT
GTACCGCGATGTGCAGCTACGCTTTCCCGGTCTGCCTGCTGTGCTGTATCACGGGTACCAGTCCGCGCATGAACGGGAGC
AGCAGTTTCTCAACCTAGCGGCACAGGCGCGCGAAGGCCGTCCGGTGCTCATAGCGGGAACGCGTTCCGCTTTGTTTCTG
CCGGTGGCGTCGCTCGGGGCCGTGGTGCTTGATGAAGAACATGATGCCTCGTTCAAACAGGATGAACGGCTGTCGTATCA
GGCCAAGGAAGTGGCATGGTTTCGTGTGGCCCAGTCAAAAGGGGCGCTGGTGCTGGGGTCTGCGACTCCGGACGTGAAGA
CCTTTCACGCCGTGCGCGAAGGACACATGCCCGGCATTGTCCTCAGCGAGCGGGTGGGCGGTGGTACGCTGCCGGCCGTG
GAACTTGTTAACATCAAAGATAACGCGGTTAAAGAGACCATTCTGGCACCGCAGACGCTGACACGCATCAAAGAGACTGT
GACCCGGGGAGAGCAGGCGGTCATACTGCTCAACAGGCGGGGCTATGCTCCGTTGATGTATTGTCTGGACTGCGGCAGCG
TGGCCCGCTGTCCGGAGTGCGAGATAGGACTGACCTATCATAAGGGACGCGAACGGCTGGTATGCCACTACTGCGGACAT
TCTGTCCCGTTTCCTTCGCCATGCGCCAAGTGCAGAGGCGTTCACTATCTGCCTATGGGGGAAGGTACCGAAAAACTGGA
AGAAACTCTCTCCACCATGCTTCCCCACGGGAAAAAGGTACTGCGGCTGGACAGAGACAGCACCCGCAGGCCCGGCCGGA
TGGAGCAGATTCTGGCGGCATTTGCCAGAGAAGAGTCCAGCATTCTGGTGGGTACCCAGATGCTTTCCAAAGGGCATCAC
TTTCCCTCCGTCACGCTGGCGGTGGTGGCTGACGGCGATATCGGGCTTAATATGCCGGACTACAGAGCCACTGAAAGAAC
GTTTCAGCTGCTGGTACAGGCTGCCGGCAGAGCAGGCAGAGGAGAACGTCCCGGCAGTGTCATCATACAGACCCGCGACC
CTGCACACTACTGCTGGGATTATATACGTAACTGCGATTATGAAGGTTTTTTTGAAAAAGAACTTGAAGCAAGACGCAAA
CGCAGATATCCGCCTTTTGTCAAACTGGCGCTTATCCGTCTGAGCTATCCCATGGACTGGCCGCAGGGGCAGGACCGTGT
GAATGCGCTGGCTTCCGCAGTGCGGGCTTCCGGCCGCGAGCTGGGAGTCTCTGTTCTGGGACCTGCTCCGGCGCCGCTGC
CTGTGCTGCGGGGGCGCAGAAGGTTTCAGTGTCTGCTGAAGGCGTCCGACTGGAAGACCATCCGCATGCTCTTTCATGCC
GCCCGTCAGGGGGTGCCTGCCTCGTCCCGGCTGCGCCTGAGTCTCGACCTTGATCCCGTGAATATGCTGTAA

Upstream 100 bases:

>100_bases
CATATATTTTGCGTTGCAGGAGGAAGACCTGCGCGATGAACTGGTGGAGCGGCTGCGTCCTTTGCTTCCCTTCAAATGTT
GACGCAGGGGCGGCTTTTTT

Downstream 100 bases:

>100_bases
CCGTATAAAAGGAGTTCCGCTGTGATCTGTAGCGTAAAAATATGTTCCGGCTGTCCGTTTTATCAGCGTGGTGAGATTTC
TTACTGTCGTGCCGACGCCC

Product: replication restart DNA helicase PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 823; Mature: 823

Protein sequence:

>823_residues
MILCGRRCRPLFFVTSGNRQLVMNENLRIVSVSLMAPPYTTLHYRLPEWLPEEMWCAGLRVAVPLGARGTLRAGVIAACG
CAPDTVPQGVELKAVLWPLEFVPLLGEEYLEMVRQLAVRQMVPPGQILGMLLPAGLRSAGVRLRVRAGGRPRTLRLRDIA
KMADSERHVLASHWSAGRVEVLEPETDEAENDICHVTADPPWPVRPAARRQTEVLEYLWENGPVSRRRMLHDLGTGAQTP
LLTLAERGLVHVGPLDAEQAEVCGCGIDSACHTEAFENQLFELTDAQQAALRACTDAVESDSAETRLLFGITGSGKTAVY
LELARACLEKGLSVMLLAPEVALACKLYRDVQLRFPGLPAVLYHGYQSAHEREQQFLNLAAQAREGRPVLIAGTRSALFL
PVASLGAVVLDEEHDASFKQDERLSYQAKEVAWFRVAQSKGALVLGSATPDVKTFHAVREGHMPGIVLSERVGGGTLPAV
ELVNIKDNAVKETILAPQTLTRIKETVTRGEQAVILLNRRGYAPLMYCLDCGSVARCPECEIGLTYHKGRERLVCHYCGH
SVPFPSPCAKCRGVHYLPMGEGTEKLEETLSTMLPHGKKVLRLDRDSTRRPGRMEQILAAFAREESSILVGTQMLSKGHH
FPSVTLAVVADGDIGLNMPDYRATERTFQLLVQAAGRAGRGERPGSVIIQTRDPAHYCWDYIRNCDYEGFFEKELEARRK
RRYPPFVKLALIRLSYPMDWPQGQDRVNALASAVRASGRELGVSVLGPAPAPLPVLRGRRRFQCLLKASDWKTIRMLFHA
ARQGVPASSRLRLSLDLDPVNML

Sequences:

>Translated_823_residues
MILCGRRCRPLFFVTSGNRQLVMNENLRIVSVSLMAPPYTTLHYRLPEWLPEEMWCAGLRVAVPLGARGTLRAGVIAACG
CAPDTVPQGVELKAVLWPLEFVPLLGEEYLEMVRQLAVRQMVPPGQILGMLLPAGLRSAGVRLRVRAGGRPRTLRLRDIA
KMADSERHVLASHWSAGRVEVLEPETDEAENDICHVTADPPWPVRPAARRQTEVLEYLWENGPVSRRRMLHDLGTGAQTP
LLTLAERGLVHVGPLDAEQAEVCGCGIDSACHTEAFENQLFELTDAQQAALRACTDAVESDSAETRLLFGITGSGKTAVY
LELARACLEKGLSVMLLAPEVALACKLYRDVQLRFPGLPAVLYHGYQSAHEREQQFLNLAAQAREGRPVLIAGTRSALFL
PVASLGAVVLDEEHDASFKQDERLSYQAKEVAWFRVAQSKGALVLGSATPDVKTFHAVREGHMPGIVLSERVGGGTLPAV
ELVNIKDNAVKETILAPQTLTRIKETVTRGEQAVILLNRRGYAPLMYCLDCGSVARCPECEIGLTYHKGRERLVCHYCGH
SVPFPSPCAKCRGVHYLPMGEGTEKLEETLSTMLPHGKKVLRLDRDSTRRPGRMEQILAAFAREESSILVGTQMLSKGHH
FPSVTLAVVADGDIGLNMPDYRATERTFQLLVQAAGRAGRGERPGSVIIQTRDPAHYCWDYIRNCDYEGFFEKELEARRK
RRYPPFVKLALIRLSYPMDWPQGQDRVNALASAVRASGRELGVSVLGPAPAPLPVLRGRRRFQCLLKASDWKTIRMLFHA
ARQGVPASSRLRLSLDLDPVNML
>Mature_823_residues
MILCGRRCRPLFFVTSGNRQLVMNENLRIVSVSLMAPPYTTLHYRLPEWLPEEMWCAGLRVAVPLGARGTLRAGVIAACG
CAPDTVPQGVELKAVLWPLEFVPLLGEEYLEMVRQLAVRQMVPPGQILGMLLPAGLRSAGVRLRVRAGGRPRTLRLRDIA
KMADSERHVLASHWSAGRVEVLEPETDEAENDICHVTADPPWPVRPAARRQTEVLEYLWENGPVSRRRMLHDLGTGAQTP
LLTLAERGLVHVGPLDAEQAEVCGCGIDSACHTEAFENQLFELTDAQQAALRACTDAVESDSAETRLLFGITGSGKTAVY
LELARACLEKGLSVMLLAPEVALACKLYRDVQLRFPGLPAVLYHGYQSAHEREQQFLNLAAQAREGRPVLIAGTRSALFL
PVASLGAVVLDEEHDASFKQDERLSYQAKEVAWFRVAQSKGALVLGSATPDVKTFHAVREGHMPGIVLSERVGGGTLPAV
ELVNIKDNAVKETILAPQTLTRIKETVTRGEQAVILLNRRGYAPLMYCLDCGSVARCPECEIGLTYHKGRERLVCHYCGH
SVPFPSPCAKCRGVHYLPMGEGTEKLEETLSTMLPHGKKVLRLDRDSTRRPGRMEQILAAFAREESSILVGTQMLSKGHH
FPSVTLAVVADGDIGLNMPDYRATERTFQLLVQAAGRAGRGERPGSVIIQTRDPAHYCWDYIRNCDYEGFFEKELEARRK
RRYPPFVKLALIRLSYPMDWPQGQDRVNALASAVRASGRELGVSVLGPAPAPLPVLRGRRRFQCLLKASDWKTIRMLFHA
ARQGVPASSRLRLSLDLDPVNML

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=558, Percent_Identity=35.84229390681, Blast_Score=335, Evalue=5e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 90948; Mature: 90948

Theoretical pI: Translated: 8.35; Mature: 8.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILCGRRCRPLFFVTSGNRQLVMNENLRIVSVSLMAPPYTTLHYRLPEWLPEEMWCAGLR
CEECCCCCCEEEEEECCCEEEEEECCEEEEEEEEECCCCCEEEECCCCCCCHHHHHCCCE
VAVPLGARGTLRAGVIAACGCAPDTVPQGVELKAVLWPLEFVPLLGEEYLEMVRQLAVRQ
EEEECCCCCCHHHCHHEECCCCCCCCCCCCEEEEEECCHHHHHHCCHHHHHHHHHHHHHH
MVPPGQILGMLLPAGLRSAGVRLRVRAGGRPRTLRLRDIAKMADSERHVLASHWSAGRVE
CCCCHHHHHHHHHCCHHCCCEEEEEECCCCCCEEHHHHHHHHCCCCCEEEECCCCCCCEE
VLEPETDEAENDICHVTADPPWPVRPAARRQTEVLEYLWENGPVSRRRMLHDLGTGAQTP
EECCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCH
LLTLAERGLVHVGPLDAEQAEVCGCGIDSACHTEAFENQLFELTDAQQAALRACTDAVES
HHHHHHCCEEEECCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCC
DSAETRLLFGITGSGKTAVYLELARACLEKGLSVMLLAPEVALACKLYRDVQLRFPGLPA
CCCCEEEEEEEECCCCEEHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHEEECCCCHH
VLYHGYQSAHEREQQFLNLAAQAREGRPVLIAGTRSALFLPVASLGAVVLDEEHDASFKQ
HHHHCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEHHHHHCCEEEEECCCCCCCCC
DERLSYQAKEVAWFRVAQSKGALVLGSATPDVKTFHAVREGHMPGIVLSERVGGGTLPAV
CHHCCHHHHHHEEEEEECCCCCEEEECCCCCHHHHHHHHHCCCCCEEEECCCCCCCCCEE
ELVNIKDNAVKETILAPQTLTRIKETVTRGEQAVILLNRRGYAPLMYCLDCGSVARCPEC
EEEECCCHHHHHHHCCHHHHHHHHHHHHCCCEEEEEEECCCCCHHHEEECCCCCCCCCCC
EIGLTYHKGRERLVCHYCGHSVPFPSPCAKCRGVHYLPMGEGTEKLEETLSTMLPHGKKV
CCCCEEECCCCEEEEEECCCCCCCCCHHHHHCCCEEEECCCCHHHHHHHHHHHCCCCCEE
LRLDRDSTRRPGRMEQILAAFAREESSILVGTQMLSKGHHFPSVTLAVVADGDIGLNMPD
EEECCCCCCCCCHHHHHHHHHHCCCCCEEEEHHHHHCCCCCCCEEEEEEECCCCCCCCCC
YRATERTFQLLVQAAGRAGRGERPGSVIIQTRDPAHYCWDYIRNCDYEGFFEKELEARRK
CHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCHHHHHHHHHCCCCCCHHHHHHHHHHH
RRYPPFVKLALIRLSYPMDWPQGQDRVNALASAVRASGRELGVSVLGPAPAPLPVLRGRR
HCCCCHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHCCHHCCEEEECCCCCCCHHHCCCC
RFQCLLKASDWKTIRMLFHAARQGVPASSRLRLSLDLDPVNML
CEEEEEECCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCC
>Mature Secondary Structure
MILCGRRCRPLFFVTSGNRQLVMNENLRIVSVSLMAPPYTTLHYRLPEWLPEEMWCAGLR
CEECCCCCCEEEEEECCCEEEEEECCEEEEEEEEECCCCCEEEECCCCCCCHHHHHCCCE
VAVPLGARGTLRAGVIAACGCAPDTVPQGVELKAVLWPLEFVPLLGEEYLEMVRQLAVRQ
EEEECCCCCCHHHCHHEECCCCCCCCCCCCEEEEEECCHHHHHHCCHHHHHHHHHHHHHH
MVPPGQILGMLLPAGLRSAGVRLRVRAGGRPRTLRLRDIAKMADSERHVLASHWSAGRVE
CCCCHHHHHHHHHCCHHCCCEEEEEECCCCCCEEHHHHHHHHCCCCCEEEECCCCCCCEE
VLEPETDEAENDICHVTADPPWPVRPAARRQTEVLEYLWENGPVSRRRMLHDLGTGAQTP
EECCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCH
LLTLAERGLVHVGPLDAEQAEVCGCGIDSACHTEAFENQLFELTDAQQAALRACTDAVES
HHHHHHCCEEEECCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCC
DSAETRLLFGITGSGKTAVYLELARACLEKGLSVMLLAPEVALACKLYRDVQLRFPGLPA
CCCCEEEEEEEECCCCEEHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHEEECCCCHH
VLYHGYQSAHEREQQFLNLAAQAREGRPVLIAGTRSALFLPVASLGAVVLDEEHDASFKQ
HHHHCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEHHHHHCCEEEEECCCCCCCCC
DERLSYQAKEVAWFRVAQSKGALVLGSATPDVKTFHAVREGHMPGIVLSERVGGGTLPAV
CHHCCHHHHHHEEEEEECCCCCEEEECCCCCHHHHHHHHHCCCCCEEEECCCCCCCCCEE
ELVNIKDNAVKETILAPQTLTRIKETVTRGEQAVILLNRRGYAPLMYCLDCGSVARCPEC
EEEECCCHHHHHHHCCHHHHHHHHHHHHCCCEEEEEEECCCCCHHHEEECCCCCCCCCCC
EIGLTYHKGRERLVCHYCGHSVPFPSPCAKCRGVHYLPMGEGTEKLEETLSTMLPHGKKV
CCCCEEECCCCEEEEEECCCCCCCCCHHHHHCCCEEEECCCCHHHHHHHHHHHCCCCCEE
LRLDRDSTRRPGRMEQILAAFAREESSILVGTQMLSKGHHFPSVTLAVVADGDIGLNMPD
EEECCCCCCCCCHHHHHHHHHHCCCCCEEEEHHHHHCCCCCCCEEEEEEECCCCCCCCCC
YRATERTFQLLVQAAGRAGRGERPGSVIIQTRDPAHYCWDYIRNCDYEGFFEKELEARRK
CHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCHHHHHHHHHCCCCCCHHHHHHHHHHH
RRYPPFVKLALIRLSYPMDWPQGQDRVNALASAVRASGRELGVSVLGPAPAPLPVLRGRR
HCCCCHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHCCHHCCEEEECCCCCCCHHHCCCC
RFQCLLKASDWKTIRMLFHAARQGVPASSRLRLSLDLDPVNML
CEEEEEECCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377; 9086272 [H]