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The map label for this gene is nth [H]

Identifier: 78356536

GI number: 78356536

Start: 1512538

End: 1513218

Strand: Direct

Name: nth [H]

Synonym: Dde_1491

Alternate gene names: 78356536

Gene position: 1512538-1513218 (Clockwise)

Preceding gene: 78356533

Following gene: 78356537

Centisome position: 40.55

GC content: 59.32

Gene sequence:

>681_bases
ATGAAAAAGAAAGAACGTGCGGCGGCTTTTCTGGCGCTGCTGAAAAAAAGGTATCCGGCTCCTGCCACGCATCTTGATGC
GCGTACCCCGTGGGAACTGCTGGTGGCTACTGTGCTTGCCGCGCAATGCACGGACGAAAGGGTGAATAAAGTCACCCCCG
GGCTTTTCCGGCGTTGGCCGGGCCCTGCCGAGCTTGCGCAGGCCTTGCAGGGAGAGGTGGAAGAAGTTGTGCATTCCACC
GGTTTTTACCGCAACAAGGCAAAAAATCTCATAGCCGCGGCAGATATGGTAACCCGCCTGCACGGCGGGCAGGTTCCGCG
TACCATGGATGAGCTGACGGCGCTGCCCGGGCTGGCCCGCAAAACGGCTAACATCGTGCTTTGGGGCGGATACGGCATTA
ACGAGGGGCTGGCGGTGGACACGCATGTGAAGCGTATAGCCTTCCGCATGGGGTTCACCGCATCGGATAATCCGGTTGTG
GTTGAAAAAGACCTGATGCCCCTTTTTCCCCGTGCGGAGTGGGGCGACGTGAACCACCGCATGGTGTGGTTCGGGCGGCA
TGTGTGCGATGCCCGCAAACCGTTGTGCCATGAGTGTGAAATGTTTGATTTCTGCCCGCGCAAAGGGGTGCAGGGAAAAA
AAACGGCAGCGCGTGCGTCTGCCCGCAAGGCCGGTGTCTGA

Upstream 100 bases:

>100_bases
ATTTCGGGGTGCAAAGCCCTGTGCCGCGGATTCTTTTCGGGGATATTGTATGCCCGTATGCTTTGCACAGTATGATTTTC
ATCCTCAGGTGGTCATGTAA

Downstream 100 bases:

>100_bases
TGCTTAAAAATACGTTCTGTCATCTGCCGGGAGTCGGTGCAGGCACTGAACGCAGCCTGTGGGACAAGGGTGTGCTGACC
TGGGAAGATGCGCTGGATGC

Product: endonuclease III / DNA-(apurinic or apyrimidinic site) lyase

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 226; Mature: 226

Protein sequence:

>226_residues
MKKKERAAAFLALLKKRYPAPATHLDARTPWELLVATVLAAQCTDERVNKVTPGLFRRWPGPAELAQALQGEVEEVVHST
GFYRNKAKNLIAAADMVTRLHGGQVPRTMDELTALPGLARKTANIVLWGGYGINEGLAVDTHVKRIAFRMGFTASDNPVV
VEKDLMPLFPRAEWGDVNHRMVWFGRHVCDARKPLCHECEMFDFCPRKGVQGKKTAARASARKAGV

Sequences:

>Translated_226_residues
MKKKERAAAFLALLKKRYPAPATHLDARTPWELLVATVLAAQCTDERVNKVTPGLFRRWPGPAELAQALQGEVEEVVHST
GFYRNKAKNLIAAADMVTRLHGGQVPRTMDELTALPGLARKTANIVLWGGYGINEGLAVDTHVKRIAFRMGFTASDNPVV
VEKDLMPLFPRAEWGDVNHRMVWFGRHVCDARKPLCHECEMFDFCPRKGVQGKKTAARASARKAGV
>Mature_226_residues
MKKKERAAAFLALLKKRYPAPATHLDARTPWELLVATVLAAQCTDERVNKVTPGLFRRWPGPAELAQALQGEVEEVVHST
GFYRNKAKNLIAAADMVTRLHGGQVPRTMDELTALPGLARKTANIVLWGGYGINEGLAVDTHVKRIAFRMGFTASDNPVV
VEKDLMPLFPRAEWGDVNHRMVWFGRHVCDARKPLCHECEMFDFCPRKGVQGKKTAARASARKAGV

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=206, Percent_Identity=28.6407766990291, Blast_Score=98, Evalue=5e-21,
Organism=Homo sapiens, GI115298654, Length=200, Percent_Identity=28.5, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI115298652, Length=200, Percent_Identity=28.5, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI6912520, Length=203, Percent_Identity=29.064039408867, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI190358497, Length=200, Percent_Identity=28.5, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI115298648, Length=200, Percent_Identity=28.5, Blast_Score=69, Evalue=2e-12,
Organism=Homo sapiens, GI115298650, Length=200, Percent_Identity=28.5, Blast_Score=69, Evalue=2e-12,
Organism=Escherichia coli, GI1787920, Length=206, Percent_Identity=36.4077669902913, Blast_Score=134, Evalue=3e-33,
Organism=Escherichia coli, GI1789331, Length=184, Percent_Identity=30.9782608695652, Blast_Score=69, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17554540, Length=196, Percent_Identity=30.6122448979592, Blast_Score=106, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6319304, Length=143, Percent_Identity=27.972027972028, Blast_Score=72, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6324530, Length=207, Percent_Identity=22.7053140096618, Blast_Score=70, Evalue=3e-13,
Organism=Drosophila melanogaster, GI45550361, Length=192, Percent_Identity=31.25, Blast_Score=105, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 25083; Mature: 25083

Theoretical pI: Translated: 10.33; Mature: 10.33

Prosite motif: PS00764 ENDONUCLEASE_III_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKERAAAFLALLKKRYPAPATHLDARTPWELLVATVLAAQCTDERVNKVTPGLFRRWP
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHCCC
GPAELAQALQGEVEEVVHSTGFYRNKAKNLIAAADMVTRLHGGQVPRTMDELTALPGLAR
CHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHH
KTANIVLWGGYGINEGLAVDTHVKRIAFRMGFTASDNPVVVEKDLMPLFPRAEWGDVNHR
CCCCEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCE
MVWFGRHVCDARKPLCHECEMFDFCPRKGVQGKKTAARASARKAGV
EEEHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MKKKERAAAFLALLKKRYPAPATHLDARTPWELLVATVLAAQCTDERVNKVTPGLFRRWP
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHCCC
GPAELAQALQGEVEEVVHSTGFYRNKAKNLIAAADMVTRLHGGQVPRTMDELTALPGLAR
CHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHH
KTANIVLWGGYGINEGLAVDTHVKRIAFRMGFTASDNPVVVEKDLMPLFPRAEWGDVNHR
CCCCEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCE
MVWFGRHVCDARKPLCHECEMFDFCPRKGVQGKKTAARASARKAGV
EEEHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]