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The map label for this gene is rlmL [H]

Identifier: 78356533

GI number: 78356533

Start: 1509843

End: 1511024

Strand: Direct

Name: rlmL [H]

Synonym: Dde_1488

Alternate gene names: 78356533

Gene position: 1509843-1511024 (Clockwise)

Preceding gene: 78356532

Following gene: 78356536

Centisome position: 40.48

GC content: 58.97

Gene sequence:

>1182_bases
ATGGCTGATTTTTCACATAAAAGCACTGTGCTTGTAACGTGTCCGCTGGCCGCTTCGGCCTTGCTGGCGACGGAACTGAA
GGCGCTGGGTTTTACGCCCCGCAATGAACTGCCCGCCGGTGTGGAGGTGCAGGCCGACCTGAATGACTGCATGCGGATGA
ACCTGCACCTGCGCACGGCCAACAGGGTGCTGTATCAGGTAGCCGCGTTTCGTGCCCGTGATGCCGATGCGCTGTACAGG
GCGGCTTCGCGCATTCACTGGGAACTGTTTATCGCCCCTGAAGGGTATGTGAGCATAGCGTCTTCCGTTGACAACCCCAC
CATACGTGACAGCCGTTTTGCCAATCAGCGGCTGAAGGATGCGGTGGTGGACAGGATACGGAGCCGTACGGGCAGACGCC
CCGATTCGGGACCGCTGCAGACGGGGGTAGTGCTGTTTCTGCACTGGCATGATGACTGGTGCCGGCTGTATGTCGATACC
TCCGGCGAGCCGCTTTCACGCAGGGGATACAGAAAAAATCCGTGGAAGGCCCCCATGCAGGAAACTCTGGCGGCCGCTGT
TCTGATGGCTGCCGGCTGGCCGGGCGGTGGGCATCTGATCAACCCCATGTGCGGTTCGGGTACTCTGGCTGTGGAAGCCG
TGCTCATGGCCATGAACTCCGCACCGGGGCTGCTGCGTGATTCTTTCTGCTTCATGCATCTGCCCGGTTATAAACCGGAG
ATATGGGACGCCCTGCTTGACAGGGCGGAAGCGCAGGAAACAGGAGAGATACCGGGGCGGGTCATTGCAACCGACATTGT
GCCCGAGGCGGTAAAGGCTTCGCAGGAAAACGCGGTACGGGCCGGTGTTGCCGGTTTTATCGAGTTCGGCGTCTGTGATT
TTACGCAGACCGACATTCCTCAGCCGTCTGAAGGGCGCGATGTGATCATCATGAATCCGGAGTACGGCGCCCGTCTCGGA
GATGCTGCGCAGCTGGAGGGTGTCTACCGGGCTGTGGGTGATTTTTTCAAACAGCGCTGCGGCGGATACACGGGGTATGT
TTTTACCGGCAACATGAAACTGGCCGGTAACGTGGGACTGCGCACTTCGCGCCGCATTCCTTTTCACAGTGCCAAAATAG
AATGCCGCCTGCTGGAATACGAGTTGTATTCCGGCAGCAGAAAGCATGCGGACCATCAGTGA

Upstream 100 bases:

>100_bases
TCACGACTATGCCGCACTGAAGCAGGACCTGCTGCGCAAGGCCGCAGCGCTTGCCGCGAGCGTGGCGTGACGGAGCAATA
CGTAAGGAATTTTGCAGCTG

Downstream 100 bases:

>100_bases
CGCCGCAGGGGTACTGCCGCGCAACCGCCATAAAAAAGAGGCTGCCACGTCATACGCGGCAGCCTCTTTTTTTATGCAAG
ACGGTCTGTTACGCCCCGAA

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL [H]

Number of amino acids: Translated: 393; Mature: 392

Protein sequence:

>393_residues
MADFSHKSTVLVTCPLAASALLATELKALGFTPRNELPAGVEVQADLNDCMRMNLHLRTANRVLYQVAAFRARDADALYR
AASRIHWELFIAPEGYVSIASSVDNPTIRDSRFANQRLKDAVVDRIRSRTGRRPDSGPLQTGVVLFLHWHDDWCRLYVDT
SGEPLSRRGYRKNPWKAPMQETLAAAVLMAAGWPGGGHLINPMCGSGTLAVEAVLMAMNSAPGLLRDSFCFMHLPGYKPE
IWDALLDRAEAQETGEIPGRVIATDIVPEAVKASQENAVRAGVAGFIEFGVCDFTQTDIPQPSEGRDVIIMNPEYGARLG
DAAQLEGVYRAVGDFFKQRCGGYTGYVFTGNMKLAGNVGLRTSRRIPFHSAKIECRLLEYELYSGSRKHADHQ

Sequences:

>Translated_393_residues
MADFSHKSTVLVTCPLAASALLATELKALGFTPRNELPAGVEVQADLNDCMRMNLHLRTANRVLYQVAAFRARDADALYR
AASRIHWELFIAPEGYVSIASSVDNPTIRDSRFANQRLKDAVVDRIRSRTGRRPDSGPLQTGVVLFLHWHDDWCRLYVDT
SGEPLSRRGYRKNPWKAPMQETLAAAVLMAAGWPGGGHLINPMCGSGTLAVEAVLMAMNSAPGLLRDSFCFMHLPGYKPE
IWDALLDRAEAQETGEIPGRVIATDIVPEAVKASQENAVRAGVAGFIEFGVCDFTQTDIPQPSEGRDVIIMNPEYGARLG
DAAQLEGVYRAVGDFFKQRCGGYTGYVFTGNMKLAGNVGLRTSRRIPFHSAKIECRLLEYELYSGSRKHADHQ
>Mature_392_residues
ADFSHKSTVLVTCPLAASALLATELKALGFTPRNELPAGVEVQADLNDCMRMNLHLRTANRVLYQVAAFRARDADALYRA
ASRIHWELFIAPEGYVSIASSVDNPTIRDSRFANQRLKDAVVDRIRSRTGRRPDSGPLQTGVVLFLHWHDDWCRLYVDTS
GEPLSRRGYRKNPWKAPMQETLAAAVLMAAGWPGGGHLINPMCGSGTLAVEAVLMAMNSAPGLLRDSFCFMHLPGYKPEI
WDALLDRAEAQETGEIPGRVIATDIVPEAVKASQENAVRAGVAGFIEFGVCDFTQTDIPQPSEGRDVIIMNPEYGARLGD
AAQLEGVYRAVGDFFKQRCGGYTGYVFTGNMKLAGNVGLRTSRRIPFHSAKIECRLLEYELYSGSRKHADHQ

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA [H]

COG id: COG0116

COG function: function code L; Predicted N6-adenine-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1787181, Length=348, Percent_Identity=28.448275862069, Blast_Score=155, Evalue=6e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114 [H]

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020 [H]

EC number: =2.1.1.173 [H]

Molecular weight: Translated: 43286; Mature: 43155

Theoretical pI: Translated: 7.52; Mature: 7.52

Prosite motif: PS51165 THUMP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADFSHKSTVLVTCPLAASALLATELKALGFTPRNELPAGVEVQADLNDCMRMNLHLRTA
CCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECHHHHHEEEEEEEHH
NRVLYQVAAFRARDADALYRAASRIHWELFIAPEGYVSIASSVDNPTIRDSRFANQRLKD
HHHHHHHHHHHCCCHHHHHHHHHCEEEEEEECCCCHHHHHHCCCCCCCCCHHHHHHHHHH
AVVDRIRSRTGRRPDSGPLQTGVVLFLHWHDDWCRLYVDTSGEPLSRRGYRKNPWKAPMQ
HHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCEEEEEECCCCCCHHHCCCCCCCCCCCHH
ETLAAAVLMAAGWPGGGHLINPMCGSGTLAVEAVLMAMNSAPGLLRDSFCFMHLPGYKPE
HHHHHHHHHHCCCCCCCCEECCCCCCCHHHHHHHHHHHCCCCCCHHCCEEEEECCCCCHH
IWDALLDRAEAQETGEIPGRVIATDIVPEAVKASQENAVRAGVAGFIEFGVCDFTQTDIP
HHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCC
QPSEGRDVIIMNPEYGARLGDAAQLEGVYRAVGDFFKQRCGGYTGYVFTGNMKLAGNVGL
CCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEEECCEEEECCCCC
RTSRRIPFHSAKIECRLLEYELYSGSRKHADHQ
CCCCCCCCCCCEEEEEEEEEEECCCCCCCCCCC
>Mature Secondary Structure 
ADFSHKSTVLVTCPLAASALLATELKALGFTPRNELPAGVEVQADLNDCMRMNLHLRTA
CCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECHHHHHEEEEEEEHH
NRVLYQVAAFRARDADALYRAASRIHWELFIAPEGYVSIASSVDNPTIRDSRFANQRLKD
HHHHHHHHHHHCCCHHHHHHHHHCEEEEEEECCCCHHHHHHCCCCCCCCCHHHHHHHHHH
AVVDRIRSRTGRRPDSGPLQTGVVLFLHWHDDWCRLYVDTSGEPLSRRGYRKNPWKAPMQ
HHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCEEEEEECCCCCCHHHCCCCCCCCCCCHH
ETLAAAVLMAAGWPGGGHLINPMCGSGTLAVEAVLMAMNSAPGLLRDSFCFMHLPGYKPE
HHHHHHHHHHCCCCCCCCEECCCCCCCHHHHHHHHHHHCCCCCCHHCCEEEEECCCCCHH
IWDALLDRAEAQETGEIPGRVIATDIVPEAVKASQENAVRAGVAGFIEFGVCDFTQTDIP
HHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCC
QPSEGRDVIIMNPEYGARLGDAAQLEGVYRAVGDFFKQRCGGYTGYVFTGNMKLAGNVGL
CCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEEECCEEEECCCCC
RTSRRIPFHSAKIECRLLEYELYSGSRKHADHQ
CCCCCCCCCCCEEEEEEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA