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The map label for this gene is dnaB [H]

Identifier: 78356454

GI number: 78356454

Start: 1427543

End: 1428913

Strand: Reverse

Name: dnaB [H]

Synonym: Dde_1409

Alternate gene names: 78356454

Gene position: 1428913-1427543 (Counterclockwise)

Preceding gene: 78356455

Following gene: 78356449

Centisome position: 38.31

GC content: 54.05

Gene sequence:

>1371_bases
GTGGATCAGGTTTCTGACGACCTGCTGCGTAACGTTCCCCCCCACAGTGTAGAGGCTGAACAGGCCGTGCTCGGGGGGGT
TTTTTTACGCGGACAGGTATTCCACACGCTGGTTGATATCGTTAATGAAGACGACTTTTATCTTCCCGCTCACAAGACCA
TCTTTGAATCGTTTTCCGAGCTGTACCGCCGCAACTCACCCATTGATCTCATCACTGTAGCTGAATTTCTGAAAGATCAG
GGTAAACTGGAGATGGCGGGCGGCACCGTGTATCTGGCAGAGCTGGCCGAATCGGTCATCAGCGCCGCCAACGCGGAACA
CTATGCCACGCTTGTGCGCAACAAGTCGCTGCAGCGCTCTCTCATCGGTGCCTGCAGCGATATAATCAGCAACTGCTACG
ATGCGTCACAGGACATCGAGCAGTTGCTCGACCATTCCGAATCATCCATTTTTTCCATTTCCGAAAGGACCACGGGCAAA
GTTTTCAAAAGCTCCAAGGAACTGGTCAGCAAGGTTTTTGAAGATCTGGAAAAACGGGTGGAACGCAAGGAGCTTGTCAC
CGGGGTAACCACAGGATACTTCCGGCTGGACAGCATGACGGCAGGCCTTCAGCCTTCTGACCTTATCATCATTGCGGCGC
GTCCTTCCATGGGCAAGACCGCTTTTGTGCTGAACATGGCCATGCGTGCCGCCGTCAATGCGGGAACGCCCGTCGCCATT
TATTCGCTGGAAATGTCCATGGAATCGCTCATGATGCGTATGTTATGCGCATGGGGCAAGGTTGATCTGGGCAAGCTGCG
TAAAGGATTTCTGGACGACGAGGACTGGGCAAAGCTGTATGCTGCCGCAGACGTGCTTTCACGCGCGCCCATCTACATCG
ACGATACTCCGGCCATCACCACGCTGGATCTGCGTGCCCGTACCCGCCGCCTGCATGCCGACAAAGGCGTCGGCATGGTT
GTTGTCGACTACCTGCAGCTTATGCGTTCCAGCCGCAGAACAGATTCACGCGAACTGGAAATTTCCGATATATCCCGAAA
CCTCAAAGCGCTGGCCAAGGAACTCAATATTCCCGTCATTGCGCTTTCCCAGCTCAACCGTAAAGTGGAAGAACGCACCA
ACAAGCGTCCCATGCTCTCCGACCTTCGCGAATCGGGCGCTATTGAGCAGGATGCCGACATCATCATGTTTGTCTATCGC
GACGAGGTATATAACAAACGGGATGACAACCCCAAAAAGGGGGTTGCCGAAATCATCATCGGCAAACAGCGTAACGGACC
AACCGGCATGGCAGAGCTTGCCTACATAGGGTCATACACGGCCTTTGAAGATCTGGCCGAAGTGCCTCCGCCCTCAGAAG
AATTCCAGTAA

Upstream 100 bases:

>100_bases
TCCGCAGCAGAAGAACCCGCAGTGGAAGAAGCCGCAGAATAGCACAGCCTTTCAGGGAAACGCTCCCGCCCGCACGGGCG
GGGGCGGTCCCTTCGGGCAG

Downstream 100 bases:

>100_bases
ACACAATGACGCCGCGCTCCCTGAGAAGCGCGGCGTCATTGTTTGTTCTGGCTGCGGTTGGCACCTACAGCCTGTCGGGA
TACGGCACCCCGGGCACCTT

Product: primary replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MDQVSDDLLRNVPPHSVEAEQAVLGGVFLRGQVFHTLVDIVNEDDFYLPAHKTIFESFSELYRRNSPIDLITVAEFLKDQ
GKLEMAGGTVYLAELAESVISAANAEHYATLVRNKSLQRSLIGACSDIISNCYDASQDIEQLLDHSESSIFSISERTTGK
VFKSSKELVSKVFEDLEKRVERKELVTGVTTGYFRLDSMTAGLQPSDLIIIAARPSMGKTAFVLNMAMRAAVNAGTPVAI
YSLEMSMESLMMRMLCAWGKVDLGKLRKGFLDDEDWAKLYAAADVLSRAPIYIDDTPAITTLDLRARTRRLHADKGVGMV
VVDYLQLMRSSRRTDSRELEISDISRNLKALAKELNIPVIALSQLNRKVEERTNKRPMLSDLRESGAIEQDADIIMFVYR
DEVYNKRDDNPKKGVAEIIIGKQRNGPTGMAELAYIGSYTAFEDLAEVPPPSEEFQ

Sequences:

>Translated_456_residues
MDQVSDDLLRNVPPHSVEAEQAVLGGVFLRGQVFHTLVDIVNEDDFYLPAHKTIFESFSELYRRNSPIDLITVAEFLKDQ
GKLEMAGGTVYLAELAESVISAANAEHYATLVRNKSLQRSLIGACSDIISNCYDASQDIEQLLDHSESSIFSISERTTGK
VFKSSKELVSKVFEDLEKRVERKELVTGVTTGYFRLDSMTAGLQPSDLIIIAARPSMGKTAFVLNMAMRAAVNAGTPVAI
YSLEMSMESLMMRMLCAWGKVDLGKLRKGFLDDEDWAKLYAAADVLSRAPIYIDDTPAITTLDLRARTRRLHADKGVGMV
VVDYLQLMRSSRRTDSRELEISDISRNLKALAKELNIPVIALSQLNRKVEERTNKRPMLSDLRESGAIEQDADIIMFVYR
DEVYNKRDDNPKKGVAEIIIGKQRNGPTGMAELAYIGSYTAFEDLAEVPPPSEEFQ
>Mature_456_residues
MDQVSDDLLRNVPPHSVEAEQAVLGGVFLRGQVFHTLVDIVNEDDFYLPAHKTIFESFSELYRRNSPIDLITVAEFLKDQ
GKLEMAGGTVYLAELAESVISAANAEHYATLVRNKSLQRSLIGACSDIISNCYDASQDIEQLLDHSESSIFSISERTTGK
VFKSSKELVSKVFEDLEKRVERKELVTGVTTGYFRLDSMTAGLQPSDLIIIAARPSMGKTAFVLNMAMRAAVNAGTPVAI
YSLEMSMESLMMRMLCAWGKVDLGKLRKGFLDDEDWAKLYAAADVLSRAPIYIDDTPAITTLDLRARTRRLHADKGVGMV
VVDYLQLMRSSRRTDSRELEISDISRNLKALAKELNIPVIALSQLNRKVEERTNKRPMLSDLRESGAIEQDADIIMFVYR
DEVYNKRDDNPKKGVAEIIIGKQRNGPTGMAELAYIGSYTAFEDLAEVPPPSEEFQ

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=443, Percent_Identity=48.9841986455982, Blast_Score=411, Evalue=1e-116,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 50896; Mature: 50896

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDQVSDDLLRNVPPHSVEAEQAVLGGVFLRGQVFHTLVDIVNEDDFYLPAHKTIFESFSE
CCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
LYRRNSPIDLITVAEFLKDQGKLEMAGGTVYLAELAESVISAANAEHYATLVRNKSLQRS
HHHCCCCCHHHHHHHHHHCCCCEEECCCEEHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
LIGACSDIISNCYDASQDIEQLLDHSESSIFSISERTTGKVFKSSKELVSKVFEDLEKRV
HHHHHHHHHHHHHCCHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERKELVTGVTTGYFRLDSMTAGLQPSDLIIIAARPSMGKTAFVLNMAMRAAVNAGTPVAI
HHHHHHHHHHHHHEEECCHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEE
YSLEMSMESLMMRMLCAWGKVDLGKLRKGFLDDEDWAKLYAAADVLSRAPIYIDDTPAIT
EEHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEECCCCCEE
TLDLRARTRRLHADKGVGMVVVDYLQLMRSSRRTDSRELEISDISRNLKALAKELNIPVI
EEHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHCCCCEE
ALSQLNRKVEERTNKRPMLSDLRESGAIEQDADIIMFVYRDEVYNKRDDNPKKGVAEIII
EHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCEEEEEEEHHHHCCCCCCHHHHHHHHHE
GKQRNGPTGMAELAYIGSYTAFEDLAEVPPPSEEFQ
ECCCCCCCCHHHHHHHCCHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MDQVSDDLLRNVPPHSVEAEQAVLGGVFLRGQVFHTLVDIVNEDDFYLPAHKTIFESFSE
CCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
LYRRNSPIDLITVAEFLKDQGKLEMAGGTVYLAELAESVISAANAEHYATLVRNKSLQRS
HHHCCCCCHHHHHHHHHHCCCCEEECCCEEHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
LIGACSDIISNCYDASQDIEQLLDHSESSIFSISERTTGKVFKSSKELVSKVFEDLEKRV
HHHHHHHHHHHHHCCHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERKELVTGVTTGYFRLDSMTAGLQPSDLIIIAARPSMGKTAFVLNMAMRAAVNAGTPVAI
HHHHHHHHHHHHHEEECCHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEE
YSLEMSMESLMMRMLCAWGKVDLGKLRKGFLDDEDWAKLYAAADVLSRAPIYIDDTPAIT
EEHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEECCCCCEE
TLDLRARTRRLHADKGVGMVVVDYLQLMRSSRRTDSRELEISDISRNLKALAKELNIPVI
EEHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHCCCCEE
ALSQLNRKVEERTNKRPMLSDLRESGAIEQDADIIMFVYRDEVYNKRDDNPKKGVAEIII
EHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCEEEEEEEHHHHCCCCCCHHHHHHHHHE
GKQRNGPTGMAELAYIGSYTAFEDLAEVPPPSEEFQ
ECCCCCCCCHHHHHHHCCHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]