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The map label for this gene is thiE

Identifier: 78356424

GI number: 78356424

Start: 1397914

End: 1398561

Strand: Direct

Name: thiE

Synonym: Dde_1379

Alternate gene names: 78356424

Gene position: 1397914-1398561 (Clockwise)

Preceding gene: 78356423

Following gene: 78356425

Centisome position: 37.48

GC content: 63.12

Gene sequence:

>648_bases
ATGCAGCCGGATTATTCGGTTTATCTGGTCACTGACCGTACGCTGTGTGTCAGTGGTACGCTGGAAGATGTGGTGGCCCG
TGCCGTGCAGGGCGGCGTTACCATGGTGCAGCTGCGCGAAAAGCAGCTTTGTACCCGTGAGTTTGTCGAACTTGGCAGGG
CGCTGCGGGCGCTGCTGGCCCCCCGCGGGGTTCCGCTGCTTGTCAACGACCGGGTGGATGTGGCTCTGGCCTGCGGGGCC
GACGGGGTGCATATCGGGCAGAGCGATATCCGTTATGCTGATGCACGCAGGCTCATGGGGGCCGGTGCCGTCATCGGCGT
GTCGGTGGAATCCGTTGAACAGGCTGCGGAAGCGGAACGTTGCGGCGCCGACTATATGGGCGTGAGCCCCGTATTTGCCA
CCCCCACAAAAACAGACACTTCCGCGCCGTGGGGGCTGGAAGGGCTGCGCGGTCTGCGGCGGCGGTTTTCTTTGCCGCTG
GTGGCCATAGGCGGTATCGGTCCGGATAATGCGGCTGAGGTTTTTGAAGCGGGAGCAGACGGCATTGCTGTTGTTTCTGC
CATTTGCGCCAGTCCGGACCCGCAGCAGGCGGCAGCAACACTGCGTGCCGTTGCCCGCGGAAAAAGGCGTGCGGCAGCCG
TTTTATGA

Upstream 100 bases:

>100_bases
CCAAAGGGCCGGGCAGTTTTCAGATGCACTTTATTGATGCGCTGTACGGCATGCAGTCATTTGATGTCGAAGCCGGCGTG
GAGATTGTGCGGTGACCCGT

Downstream 100 bases:

>100_bases
TCCGGCGCAGTGCTGACCGGCAGTTGCATCCGTAAACAGGGATAACAGTAAACTGGGAGGCTGTATGCTTATTGCAGTGG
CGGCGGACGGAAAAACTCTG

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 215; Mature: 215

Protein sequence:

>215_residues
MQPDYSVYLVTDRTLCVSGTLEDVVARAVQGGVTMVQLREKQLCTREFVELGRALRALLAPRGVPLLVNDRVDVALACGA
DGVHIGQSDIRYADARRLMGAGAVIGVSVESVEQAAEAERCGADYMGVSPVFATPTKTDTSAPWGLEGLRGLRRRFSLPL
VAIGGIGPDNAAEVFEAGADGIAVVSAICASPDPQQAAATLRAVARGKRRAAAVL

Sequences:

>Translated_215_residues
MQPDYSVYLVTDRTLCVSGTLEDVVARAVQGGVTMVQLREKQLCTREFVELGRALRALLAPRGVPLLVNDRVDVALACGA
DGVHIGQSDIRYADARRLMGAGAVIGVSVESVEQAAEAERCGADYMGVSPVFATPTKTDTSAPWGLEGLRGLRRRFSLPL
VAIGGIGPDNAAEVFEAGADGIAVVSAICASPDPQQAAATLRAVARGKRRAAAVL
>Mature_215_residues
MQPDYSVYLVTDRTLCVSGTLEDVVARAVQGGVTMVQLREKQLCTREFVELGRALRALLAPRGVPLLVNDRVDVALACGA
DGVHIGQSDIRYADARRLMGAGAVIGVSVESVEQAAEAERCGADYMGVSPVFATPTKTDTSAPWGLEGLRGLRRRFSLPL
VAIGGIGPDNAAEVFEAGADGIAVVSAICASPDPQQAAATLRAVARGKRRAAAVL

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=189, Percent_Identity=35.4497354497354, Blast_Score=79, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6325042, Length=226, Percent_Identity=37.1681415929204, Blast_Score=125, Evalue=5e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 22459; Mature: 22459

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPDYSVYLVTDRTLCVSGTLEDVVARAVQGGVTMVQLREKQLCTREFVELGRALRALLA
CCCCEEEEEEECCEEEECCCHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHC
PRGVPLLVNDRVDVALACGADGVHIGQSDIRYADARRLMGAGAVIGVSVESVEQAAEAER
CCCCEEEEECCCCEEEEECCCCCEECHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHH
CGADYMGVSPVFATPTKTDTSAPWGLEGLRGLRRRFSLPLVAIGGIGPDNAAEVFEAGAD
CCCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHCCCC
GIAVVSAICASPDPQQAAATLRAVARGKRRAAAVL
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQPDYSVYLVTDRTLCVSGTLEDVVARAVQGGVTMVQLREKQLCTREFVELGRALRALLA
CCCCEEEEEEECCEEEECCCHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHC
PRGVPLLVNDRVDVALACGADGVHIGQSDIRYADARRLMGAGAVIGVSVESVEQAAEAER
CCCCEEEEECCCCEEEEECCCCCEECHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHH
CGADYMGVSPVFATPTKTDTSAPWGLEGLRGLRRRFSLPLVAIGGIGPDNAAEVFEAGAD
CCCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHCCCC
GIAVVSAICASPDPQQAAATLRAVARGKRRAAAVL
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA