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The map label for this gene is thiM

Identifier: 78356423

GI number: 78356423

Start: 1397108

End: 1397908

Strand: Direct

Name: thiM

Synonym: Dde_1378

Alternate gene names: 78356423

Gene position: 1397108-1397908 (Clockwise)

Preceding gene: 78356422

Following gene: 78356424

Centisome position: 37.45

GC content: 60.92

Gene sequence:

>801_bases
ATGAGCGAACATAGCTTCGTCTGGGACGCGGTACAGCGCGTCCGTACCGGTGCTCCGCTGGTGCATAATATAACCAACTA
TGTAGTGATGAATACCACCGCAAACGCGCTGCTGGCCGCCGGTGCATCCCCCGTCATGGCTCATGCGCGGGAAGAGGTGG
AAGAACTGGCCGGTCTGTGCTCTTCTCTGGTGCTGAATATCGGCACCCTGAGCAAACCGTGGATAGAGTCCATGTTCGCA
GCCGGACGCATTGCGCAGTCGCGCGGTGTGCCTGTGGTGCTCGATCCCGTGGGGGCTGGGGCGTCTTCGCTGCGTACATG
GACAAGCCTTGATCTGCTGCGCGAGCTCAATATAAGCGTACTGCGCGGTAATGCCTCTGAAATTCTGGCACTTTCCGGTG
CCGGATCACGCACGAAAGGGGTGGACAGCATACATCAGGCTGCCGATGCCGCCGATGCCGCACGTGAACTTGCCGGTAAG
TTCGGTTGCGTGGTTGTTGTCAGCGGGGCGGAAGATCTGATTACCGACGGTTCCAGCGACGTGCTGGTGCGCGGCGGACA
CGACATGATGCCCCGTATTACCGGCATGGGCTGCACGGCCACTGCTCTGGTGGCGGCGCATGCGGCTGTTGCCGGCAGTG
TGCTGGACGGCGCGGCCGCAGGCATGGGAGTTATGGCCGTGGCGGGTACCATGGCCTCGCGCAAGGCCAAAGGGCCGGGC
AGTTTTCAGATGCACTTTATTGATGCGCTGTACGGCATGCAGTCATTTGATGTCGAAGCCGGCGTGGAGATTGTGCGGTG
A

Upstream 100 bases:

>100_bases
CGCATCCTGCAATACTTTTTAGAGCATGCAATGGACGTGACCGGCTGTCCGCGCTACAGTGAGTGCGTGAAAACAGTGAT
AAATAAGGAGAAGATTTTCA

Downstream 100 bases:

>100_bases
CCCGTATGCAGCCGGATTATTCGGTTTATCTGGTCACTGACCGTACGCTGTGTGTCAGTGGTACGCTGGAAGATGTGGTG
GCCCGTGCCGTGCAGGGCGG

Product: hydroxyethylthiazole kinase

Products: NA

Alternate protein names: 4-methyl-5-beta-hydroxyethylthiazole kinase; TH kinase; Thz kinase

Number of amino acids: Translated: 266; Mature: 265

Protein sequence:

>266_residues
MSEHSFVWDAVQRVRTGAPLVHNITNYVVMNTTANALLAAGASPVMAHAREEVEELAGLCSSLVLNIGTLSKPWIESMFA
AGRIAQSRGVPVVLDPVGAGASSLRTWTSLDLLRELNISVLRGNASEILALSGAGSRTKGVDSIHQAADAADAARELAGK
FGCVVVVSGAEDLITDGSSDVLVRGGHDMMPRITGMGCTATALVAAHAAVAGSVLDGAAAGMGVMAVAGTMASRKAKGPG
SFQMHFIDALYGMQSFDVEAGVEIVR

Sequences:

>Translated_266_residues
MSEHSFVWDAVQRVRTGAPLVHNITNYVVMNTTANALLAAGASPVMAHAREEVEELAGLCSSLVLNIGTLSKPWIESMFA
AGRIAQSRGVPVVLDPVGAGASSLRTWTSLDLLRELNISVLRGNASEILALSGAGSRTKGVDSIHQAADAADAARELAGK
FGCVVVVSGAEDLITDGSSDVLVRGGHDMMPRITGMGCTATALVAAHAAVAGSVLDGAAAGMGVMAVAGTMASRKAKGPG
SFQMHFIDALYGMQSFDVEAGVEIVR
>Mature_265_residues
SEHSFVWDAVQRVRTGAPLVHNITNYVVMNTTANALLAAGASPVMAHAREEVEELAGLCSSLVLNIGTLSKPWIESMFAA
GRIAQSRGVPVVLDPVGAGASSLRTWTSLDLLRELNISVLRGNASEILALSGAGSRTKGVDSIHQAADAADAARELAGKF
GCVVVVSGAEDLITDGSSDVLVRGGHDMMPRITGMGCTATALVAAHAAVAGSVLDGAAAGMGVMAVAGTMASRKAKGPGS
FQMHFIDALYGMQSFDVEAGVEIVR

Specific function: Thiamine biosynthesis. [C]

COG id: COG2145

COG function: function code H; Hydroxyethylthiazole kinase, sugar kinase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Thz kinase family

Homologues:

Organism=Escherichia coli, GI1788421, Length=244, Percent_Identity=41.8032786885246, Blast_Score=168, Evalue=4e-43,
Organism=Saccharomyces cerevisiae, GI6325042, Length=275, Percent_Identity=30.9090909090909, Blast_Score=106, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIM_DESDG (Q312G9)

Other databases:

- EMBL:   CP000112
- RefSeq:   YP_387872.1
- ProteinModelPortal:   Q312G9
- SMR:   Q312G9
- STRING:   Q312G9
- GeneID:   3756165
- GenomeReviews:   CP000112_GR
- KEGG:   dde:Dde_1378
- eggNOG:   COG2145
- HOGENOM:   HBG351126
- OMA:   AIRGNAG
- ProtClustDB:   PRK09355
- BioCyc:   DDES207559:DDE_1378-MONOMER
- HAMAP:   MF_00228
- InterPro:   IPR000417
- PANTHER:   PTHR20857:SF14
- PIRSF:   PIRSF000513
- PRINTS:   PR01099
- TIGRFAMs:   TIGR00694

Pfam domain/function: PF02110 HK

EC number: =2.7.1.50

Molecular weight: Translated: 27302; Mature: 27170

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: NA

Important sites: BINDING 46-46 BINDING 122-122 BINDING 168-168 BINDING 195-195

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEHSFVWDAVQRVRTGAPLVHNITNYVVMNTTANALLAAGASPVMAHAREEVEELAGLC
CCCCHHHHHHHHHHHCCCCHHHHHHCEEEECCCHHHHHHCCCCHHHHHHHHHHHHHHHHH
SSLVLNIGTLSKPWIESMFAAGRIAQSRGVPVVLDPVGAGASSLRTWTSLDLLRELNISV
HHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCEE
LRGNASEILALSGAGSRTKGVDSIHQAADAADAARELAGKFGCVVVVSGAEDLITDGSSD
EECCHHHEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHCCCCCC
VLVRGGHDMMPRITGMGCTATALVAAHAAVAGSVLDGAAAGMGVMAVAGTMASRKAKGPG
EEEECCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCC
SFQMHFIDALYGMQSFDVEAGVEIVR
CHHHHHHHHHHCCHHCCHHCCCEECC
>Mature Secondary Structure 
SEHSFVWDAVQRVRTGAPLVHNITNYVVMNTTANALLAAGASPVMAHAREEVEELAGLC
CCCHHHHHHHHHHHCCCCHHHHHHCEEEECCCHHHHHHCCCCHHHHHHHHHHHHHHHHH
SSLVLNIGTLSKPWIESMFAAGRIAQSRGVPVVLDPVGAGASSLRTWTSLDLLRELNISV
HHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCEE
LRGNASEILALSGAGSRTKGVDSIHQAADAADAARELAGKFGCVVVVSGAEDLITDGSSD
EECCHHHEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHCCCCCC
VLVRGGHDMMPRITGMGCTATALVAAHAAVAGSVLDGAAAGMGVMAVAGTMASRKAKGPG
EEEECCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCC
SFQMHFIDALYGMQSFDVEAGVEIVR
CHHHHHHHHHHCCHHCCHHCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA