Click here to switch to the map view.

The map label for this gene is yiaO [H]

Identifier: 78356322

GI number: 78356322

Start: 1294265

End: 1295275

Strand: Reverse

Name: yiaO [H]

Synonym: Dde_1275

Alternate gene names: 78356322

Gene position: 1295275-1294265 (Counterclockwise)

Preceding gene: 78356327

Following gene: 78356321

Centisome position: 34.72

GC content: 56.08

Gene sequence:

>1011_bases
ATGAAACACCTATTGAAAGCAGGCGCACTGGTGGCGCTGGCGTGCATCGTGACCCTGACGGCAGGCGCACAGGCGCATGC
GGCAAAGCGTATCAACATCCGTCTGGCGCACCCCATGGCGCCGGGCAACAATGTGACGGTAGGCTACGAAAAATTTAAGG
AACTGGTTGCAGAAAAAAGCAACGGCCGTGTGCGCATCCAGCTGTTCGGCAACTGCATGCTGGGCAGCGACCGTGTGACC
ATGGAAGCCGCACAGCGCGGCACGCTGGAAATGGCTTCCAGTTCTTCACCCAACATGGCCAACTTTTCCAAACAGTGGAT
GGTGTTTGACCTGCCCTACATCACCAGCCCCGAGCATCAGCAAAAGCTGTACAAAGCCATCGATGACGGCGAACTGGGCA
AAAAGCTGGATGAAATAGCCGCCTCCATCGGTCTGAAGCCCATCATGTACAGCGAATATGGTTACCGCAATTTTGTAACC
ACTAAAAAGCCCATCAAAACCGCTGACGATCTGAAAAACCTTAAGGTGCGTACCACCGACTCACCCATTGAAGTGGCCGT
GGCTGCCGCGCTGGGCATGGCTCCCACCCCCATTTCGTGGGGCGAGACCTACACGGCTCTGCAGCAGGGCACCGTGGACG
GCGAAGGCAACACATTCTCGCTGCTCAACGACGCCAAGCACACCGAAGTGCTGAAGTACGCAATAGATTCTGCCCACAAC
TACAGCATGCACCTGCTGATGATGAACAAAGCCTACTACGACAGCCTGCCTGCCAATGTGCAGCAGATTCTGACAGAAGC
AGGCCGCGAAGCACTGACCTATCAGCGCTCCATCACCAGCGAGCTTGAAAAGAAAGCGGAAGACGCATTCATCGAACAGG
GCATCACGGTGACCAGACTGAGCCCCGAAGAGCGCGCCAAGCTGGTCGAACGCACCCGCCCCGTCTGGGACAAGTTCAAA
GACGACATTCCTGCCGAACTGATCAAACTGGTGCAGGAAACCCAGCAGTAA

Upstream 100 bases:

>100_bases
GCGCCGCTTTTGCGGTTTTGGCACATTACCTGCTCAGTAAGGGACGAAAGGCAGCTGCTAACAACACACTCGGGAAGAAC
CTCAAGACCAAGGAAGAACC

Downstream 100 bases:

>100_bases
ACAGAAACCGGGGCAGGGGGCCTGCCCGCTTCCTGCCCCGGAAAGCCGCTTTTCCGACAACGCAACCAAGAGGTCATAAT
GTCAGATCCCAACGTCACAG

Product: TRAP dicarboxylate transporter subunit DctP

Products: NA

Alternate protein names: 2,3-DKG-binding protein; Extracytoplasmic solute receptor protein yiaO [H]

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MKHLLKAGALVALACIVTLTAGAQAHAAKRINIRLAHPMAPGNNVTVGYEKFKELVAEKSNGRVRIQLFGNCMLGSDRVT
MEAAQRGTLEMASSSSPNMANFSKQWMVFDLPYITSPEHQQKLYKAIDDGELGKKLDEIAASIGLKPIMYSEYGYRNFVT
TKKPIKTADDLKNLKVRTTDSPIEVAVAAALGMAPTPISWGETYTALQQGTVDGEGNTFSLLNDAKHTEVLKYAIDSAHN
YSMHLLMMNKAYYDSLPANVQQILTEAGREALTYQRSITSELEKKAEDAFIEQGITVTRLSPEERAKLVERTRPVWDKFK
DDIPAELIKLVQETQQ

Sequences:

>Translated_336_residues
MKHLLKAGALVALACIVTLTAGAQAHAAKRINIRLAHPMAPGNNVTVGYEKFKELVAEKSNGRVRIQLFGNCMLGSDRVT
MEAAQRGTLEMASSSSPNMANFSKQWMVFDLPYITSPEHQQKLYKAIDDGELGKKLDEIAASIGLKPIMYSEYGYRNFVT
TKKPIKTADDLKNLKVRTTDSPIEVAVAAALGMAPTPISWGETYTALQQGTVDGEGNTFSLLNDAKHTEVLKYAIDSAHN
YSMHLLMMNKAYYDSLPANVQQILTEAGREALTYQRSITSELEKKAEDAFIEQGITVTRLSPEERAKLVERTRPVWDKFK
DDIPAELIKLVQETQQ
>Mature_336_residues
MKHLLKAGALVALACIVTLTAGAQAHAAKRINIRLAHPMAPGNNVTVGYEKFKELVAEKSNGRVRIQLFGNCMLGSDRVT
MEAAQRGTLEMASSSSPNMANFSKQWMVFDLPYITSPEHQQKLYKAIDDGELGKKLDEIAASIGLKPIMYSEYGYRNFVT
TKKPIKTADDLKNLKVRTTDSPIEVAVAAALGMAPTPISWGETYTALQQGTVDGEGNTFSLLNDAKHTEVLKYAIDSAHN
YSMHLLMMNKAYYDSLPANVQQILTEAGREALTYQRSITSELEKKAEDAFIEQGITVTRLSPEERAKLVERTRPVWDKFK
DDIPAELIKLVQETQQ

Specific function: Part of the tripartite ATP-independent periplasmic (TRAP) transport system yiaMNO involved in the uptake of 2,3- diketo-L-gulonate. This protein specifically binds 2,3-diketo-L- gulonate. Is not able to bind either L-ascorbate or dehydroascorbate [H]

COG id: COG1638

COG function: function code G; TRAP-type C4-dicarboxylate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 7 family [H]

Homologues:

Organism=Escherichia coli, GI1790004, Length=330, Percent_Identity=30.3030303030303, Blast_Score=160, Evalue=1e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018389
- InterPro:   IPR004682 [H]

Pfam domain/function: PF03480 SBP_bac_7 [H]

EC number: NA

Molecular weight: Translated: 37241; Mature: 37241

Theoretical pI: Translated: 7.69; Mature: 7.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHLLKAGALVALACIVTLTAGAQAHAAKRINIRLAHPMAPGNNVTVGYEKFKELVAEKS
CCHHHHHHHHHHHHHHHHHHCCCHHHHEEEEEEEEECCCCCCCCEEECHHHHHHHHHCCC
NGRVRIQLFGNCMLGSDRVTMEAAQRGTLEMASSSSPNMANFSKQWMVFDLPYITSPEHQ
CCEEEEEEEECEEECCCCHHHHHHHCCCEEECCCCCCCCCCCCCCEEEEECCCCCCCHHH
QKLYKAIDDGELGKKLDEIAASIGLKPIMYSEYGYRNFVTTKKPIKTADDLKNLKVRTTD
HHHHHHCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCHHHHHCCEEEECC
SPIEVAVAAALGMAPTPISWGETYTALQQGTVDGEGNTFSLLNDAKHTEVLKYAIDSAHN
CCHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHCCCC
YSMHLLMMNKAYYDSLPANVQQILTEAGREALTYQRSITSELEKKAEDAFIEQGITVTRL
CEEEEEEECHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEC
SPEERAKLVERTRPVWDKFKDDIPAELIKLVQETQQ
CHHHHHHHHHHHCHHHHHHHCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MKHLLKAGALVALACIVTLTAGAQAHAAKRINIRLAHPMAPGNNVTVGYEKFKELVAEKS
CCHHHHHHHHHHHHHHHHHHCCCHHHHEEEEEEEEECCCCCCCCEEECHHHHHHHHHCCC
NGRVRIQLFGNCMLGSDRVTMEAAQRGTLEMASSSSPNMANFSKQWMVFDLPYITSPEHQ
CCEEEEEEEECEEECCCCHHHHHHHCCCEEECCCCCCCCCCCCCCEEEEECCCCCCCHHH
QKLYKAIDDGELGKKLDEIAASIGLKPIMYSEYGYRNFVTTKKPIKTADDLKNLKVRTTD
HHHHHHCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCHHHHHCCEEEECC
SPIEVAVAAALGMAPTPISWGETYTALQQGTVDGEGNTFSLLNDAKHTEVLKYAIDSAHN
CCHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHCCCC
YSMHLLMMNKAYYDSLPANVQQILTEAGREALTYQRSITSELEKKAEDAFIEQGITVTRL
CEEEEEEECHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEC
SPEERAKLVERTRPVWDKFKDDIPAELIKLVQETQQ
CHHHHHHHHHHHCHHHHHHHCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503 [H]