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The map label for this gene is phoA [H]

Identifier: 78356263

GI number: 78356263

Start: 1242622

End: 1244106

Strand: Direct

Name: phoA [H]

Synonym: Dde_1216

Alternate gene names: 78356263

Gene position: 1242622-1244106 (Clockwise)

Preceding gene: 78356262

Following gene: 78356264

Centisome position: 33.31

GC content: 54.68

Gene sequence:

>1485_bases
ATGCGTCCGTCTTGTTTTCAGCGTTCCGTAAGTGTTGTCTGTATGCTGATGACGCTGCTGGTCTGCGCGCACAGCGCAGC
TGCGGCAGAGTCTTTTGCAAAGAAACCCGCAAAATATGTTTTTCTGTTTATCGGTGACGGCATGGGCCTGCCGCAAAAGC
AGGCCACAGAAGCGTTTACAGGCCGTCAGCTGGTTCTCGATTCTTTTCCGGTGCACGGCATAACCACCACCCCCGCCGCC
AACAGGTTCATTGTGGATTCCGCTGCTGCGGCCACGGCCATGAGTACCGGTCAGCTGACAGATGTGGGCATGATAGGTAT
GGCGCCGGACAAGACAAAAGTGAAGACCATTGCCGAAATGGCCCGTGAAAAGGGCATGAAAGTGGGCATTGTTTCTTCTG
TCTCCATTGATCATGCAACTCCGGCTGCGTTCTACGCTCACGAGGAAAGCCGCAACCTGTACCATTACATCGACCATGCA
CTGGCTTCGTCCGGATTTGACTACTTTGCCGGAGGAGGCATCAAGGACCCCGCGGGCAAGCGTAAAGGCGTGGAACCGCG
CGGCAATGCCATGGATGCCATTAAAAACGCCGGATACAAAGTGGTGACGGACCGCGACGAGTTTTTGAAGCTGAACCGCA
AAGACGGAAAGGTGTTTGCGTACAACAGCTGGCTGCAGGATTCCGGTGCGCTGCCCTATGCCATGGACAGCCGCCCGGGC
AAAGATATCACTCTGGCAGAATTCACCGCCAAGGGCATAGACCTGCTGGATAACAGCAAGGGCTTTTTTATGATGGTTGA
GGGAGGTAAAATTGACTGGGCCTGCCATGCCAACGATGCCAAAGCAGCCATTATGGATACTGTGGCCTTTGACGAGGCCG
TGGCCGAGGCTGTCAGATTTTACAAAAAGCATCCCGACGAGACCCTGATTGTTGTGACGGGCGACCACGAATGCGGCGGC
ATGACTCTGGGATTTGCCGGTACGGCATATGAGTCTGATTTTGACGTGCTGAAAAAACAGTATGTCTCGTTTCAGTATTT
TGCCGATGATGTGCTGAAGCTGTTTAAAAAAGAGCATCAGGGTTCGCTGGCGTTCAATGACCTGAAGCCCACCATCACAC
ATTACTTCGGGCTGCAGTTTGCAGGCGACCATGCAGACCCCATGTATGTGCAGCCCCACGAGCTTGCCCAGCTGCAGGAT
GCGTTCGTCAAGTCTGTGGCGGGCGATTCCATGAAGCTGAACGATCCTGTTTCCGTCAACCTGTACGGCGGATACGATCC
TCTCATTGTCACTATAACCCACCTGCTCAATAACAAGGCCGGGCTGGCGTGGACATCCTTCTCGCATACAGCGGTTCCCG
TGGGAACAACCGCCGTGGGCCGCGGTGCGGACCTTTTCGGCGGATCGTATCACCAGACGGCCATTGCCAAAAAGATCATG
ACGGCCATGGGTGTGAAGCCCCATGTCTATGTGGCGGCCAACTGA

Upstream 100 bases:

>100_bases
CCGTACTGTCATTCTGTTGTCACCTTCCGGGGACATATCCTTGCCAGCCAATGCGGCAGTGCCGTTTTTTTTTATTTGTT
GTCAGGCAAGGAGAATTCAC

Downstream 100 bases:

>100_bases
TCATCTGACTGTTGCAGAAACAAGGGCGGCGCGGCCTGCGGGCTGCGCCGCTGTTTTACATGCCGTGCGCGGCTTATGTG
GAGAGAACGCTATGCGCAGC

Product: alkaline phosphatase

Products: NA

Alternate protein names: Alkaline phosphatase IV; APase IV [H]

Number of amino acids: Translated: 494; Mature: 494

Protein sequence:

>494_residues
MRPSCFQRSVSVVCMLMTLLVCAHSAAAAESFAKKPAKYVFLFIGDGMGLPQKQATEAFTGRQLVLDSFPVHGITTTPAA
NRFIVDSAAAATAMSTGQLTDVGMIGMAPDKTKVKTIAEMAREKGMKVGIVSSVSIDHATPAAFYAHEESRNLYHYIDHA
LASSGFDYFAGGGIKDPAGKRKGVEPRGNAMDAIKNAGYKVVTDRDEFLKLNRKDGKVFAYNSWLQDSGALPYAMDSRPG
KDITLAEFTAKGIDLLDNSKGFFMMVEGGKIDWACHANDAKAAIMDTVAFDEAVAEAVRFYKKHPDETLIVVTGDHECGG
MTLGFAGTAYESDFDVLKKQYVSFQYFADDVLKLFKKEHQGSLAFNDLKPTITHYFGLQFAGDHADPMYVQPHELAQLQD
AFVKSVAGDSMKLNDPVSVNLYGGYDPLIVTITHLLNNKAGLAWTSFSHTAVPVGTTAVGRGADLFGGSYHQTAIAKKIM
TAMGVKPHVYVAAN

Sequences:

>Translated_494_residues
MRPSCFQRSVSVVCMLMTLLVCAHSAAAAESFAKKPAKYVFLFIGDGMGLPQKQATEAFTGRQLVLDSFPVHGITTTPAA
NRFIVDSAAAATAMSTGQLTDVGMIGMAPDKTKVKTIAEMAREKGMKVGIVSSVSIDHATPAAFYAHEESRNLYHYIDHA
LASSGFDYFAGGGIKDPAGKRKGVEPRGNAMDAIKNAGYKVVTDRDEFLKLNRKDGKVFAYNSWLQDSGALPYAMDSRPG
KDITLAEFTAKGIDLLDNSKGFFMMVEGGKIDWACHANDAKAAIMDTVAFDEAVAEAVRFYKKHPDETLIVVTGDHECGG
MTLGFAGTAYESDFDVLKKQYVSFQYFADDVLKLFKKEHQGSLAFNDLKPTITHYFGLQFAGDHADPMYVQPHELAQLQD
AFVKSVAGDSMKLNDPVSVNLYGGYDPLIVTITHLLNNKAGLAWTSFSHTAVPVGTTAVGRGADLFGGSYHQTAIAKKIM
TAMGVKPHVYVAAN
>Mature_494_residues
MRPSCFQRSVSVVCMLMTLLVCAHSAAAAESFAKKPAKYVFLFIGDGMGLPQKQATEAFTGRQLVLDSFPVHGITTTPAA
NRFIVDSAAAATAMSTGQLTDVGMIGMAPDKTKVKTIAEMAREKGMKVGIVSSVSIDHATPAAFYAHEESRNLYHYIDHA
LASSGFDYFAGGGIKDPAGKRKGVEPRGNAMDAIKNAGYKVVTDRDEFLKLNRKDGKVFAYNSWLQDSGALPYAMDSRPG
KDITLAEFTAKGIDLLDNSKGFFMMVEGGKIDWACHANDAKAAIMDTVAFDEAVAEAVRFYKKHPDETLIVVTGDHECGG
MTLGFAGTAYESDFDVLKKQYVSFQYFADDVLKLFKKEHQGSLAFNDLKPTITHYFGLQFAGDHADPMYVQPHELAQLQD
AFVKSVAGDSMKLNDPVSVNLYGGYDPLIVTITHLLNNKAGLAWTSFSHTAVPVGTTAVGRGADLFGGSYHQTAIAKKIM
TAMGVKPHVYVAAN

Specific function: Unknown

COG id: COG1785

COG function: function code P; Alkaline phosphatase

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkaline phosphatase family [H]

Homologues:

Organism=Homo sapiens, GI157266292, Length=345, Percent_Identity=28.4057971014493, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI116734717, Length=334, Percent_Identity=30.2395209580838, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI294660770, Length=329, Percent_Identity=29.7872340425532, Blast_Score=106, Evalue=4e-23,
Organism=Homo sapiens, GI157266296, Length=334, Percent_Identity=27.2455089820359, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI94721246, Length=334, Percent_Identity=26.6467065868263, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI294660772, Length=281, Percent_Identity=30.2491103202847, Blast_Score=89, Evalue=1e-17,
Organism=Escherichia coli, GI48994877, Length=351, Percent_Identity=33.3333333333333, Blast_Score=125, Evalue=9e-30,
Organism=Saccharomyces cerevisiae, GI6320689, Length=360, Percent_Identity=28.6111111111111, Blast_Score=107, Evalue=4e-24,
Organism=Drosophila melanogaster, GI85725200, Length=341, Percent_Identity=29.6187683284457, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI85815833, Length=341, Percent_Identity=29.6187683284457, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI21355149, Length=346, Percent_Identity=30.0578034682081, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24651554, Length=350, Percent_Identity=30.8571428571429, Blast_Score=119, Evalue=7e-27,
Organism=Drosophila melanogaster, GI21355981, Length=355, Percent_Identity=30.1408450704225, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI19921912, Length=342, Percent_Identity=31.5789473684211, Blast_Score=115, Evalue=8e-26,
Organism=Drosophila melanogaster, GI21355151, Length=487, Percent_Identity=25.6673511293634, Blast_Score=108, Evalue=8e-24,
Organism=Drosophila melanogaster, GI24651556, Length=306, Percent_Identity=31.0457516339869, Blast_Score=103, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24657827, Length=373, Percent_Identity=27.3458445040214, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24657835, Length=391, Percent_Identity=26.5984654731458, Blast_Score=94, Evalue=2e-19,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR001952
- InterPro:   IPR018299
- InterPro:   IPR017850 [H]

Pfam domain/function: PF00245 Alk_phosphatase [H]

EC number: =3.1.3.1 [H]

Molecular weight: Translated: 53387; Mature: 53387

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: PS00123 ALKALINE_PHOSPHATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPSCFQRSVSVVCMLMTLLVCAHSAAAAESFAKKPAKYVFLFIGDGMGLPQKQATEAFT
CCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHC
GRQLVLDSFPVHGITTTPAANRFIVDSAAAATAMSTGQLTDVGMIGMAPDKTKVKTIAEM
CCEEEEECCCCCCEECCCCCCEEEEECHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHH
AREKGMKVGIVSSVSIDHATPAAFYAHEESRNLYHYIDHALASSGFDYFAGGGIKDPAGK
HHHCCCEEEEEEEEECCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCC
RKGVEPRGNAMDAIKNAGYKVVTDRDEFLKLNRKDGKVFAYNSWLQDSGALPYAMDSRPG
CCCCCCCCCHHHHHHCCCEEEEECCHHHHEEECCCCEEEEEECCCCCCCCCCEEECCCCC
KDITLAEFTAKGIDLLDNSKGFFMMVEGGKIDWACHANDAKAAIMDTVAFDEAVAEAVRF
CCEEHHHHHCCCCCEEECCCCEEEEEECCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHH
YKKHPDETLIVVTGDHECGGMTLGFAGTAYESDFDVLKKQYVSFQYFADDVLKLFKKEHQ
HHHCCCCEEEEEECCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GSLAFNDLKPTITHYFGLQFAGDHADPMYVQPHELAQLQDAFVKSVAGDSMKLNDPVSVN
CCEEECCCCHHHHHHCCCEECCCCCCCCEECHHHHHHHHHHHHHHHCCCCEECCCCEEEE
LYGGYDPLIVTITHLLNNKAGLAWTSFSHTAVPVGTTAVGRGADLFGGSYHQTAIAKKIM
EECCCCHHHHHHHHHHCCCCCEEEECCCCCEECCCCCCCCCCCHHCCCCHHHHHHHHHHH
TAMGVKPHVYVAAN
HHHCCCCEEEEEEC
>Mature Secondary Structure
MRPSCFQRSVSVVCMLMTLLVCAHSAAAAESFAKKPAKYVFLFIGDGMGLPQKQATEAFT
CCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHC
GRQLVLDSFPVHGITTTPAANRFIVDSAAAATAMSTGQLTDVGMIGMAPDKTKVKTIAEM
CCEEEEECCCCCCEECCCCCCEEEEECHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHH
AREKGMKVGIVSSVSIDHATPAAFYAHEESRNLYHYIDHALASSGFDYFAGGGIKDPAGK
HHHCCCEEEEEEEEECCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCC
RKGVEPRGNAMDAIKNAGYKVVTDRDEFLKLNRKDGKVFAYNSWLQDSGALPYAMDSRPG
CCCCCCCCCHHHHHHCCCEEEEECCHHHHEEECCCCEEEEEECCCCCCCCCCEEECCCCC
KDITLAEFTAKGIDLLDNSKGFFMMVEGGKIDWACHANDAKAAIMDTVAFDEAVAEAVRF
CCEEHHHHHCCCCCEEECCCCEEEEEECCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHH
YKKHPDETLIVVTGDHECGGMTLGFAGTAYESDFDVLKKQYVSFQYFADDVLKLFKKEHQ
HHHCCCCEEEEEECCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GSLAFNDLKPTITHYFGLQFAGDHADPMYVQPHELAQLQDAFVKSVAGDSMKLNDPVSVN
CCEEECCCCHHHHHHCCCEECCCCCCCCEECHHHHHHHHHHHHHHHCCCCEECCCCEEEE
LYGGYDPLIVTITHLLNNKAGLAWTSFSHTAVPVGTTAVGRGADLFGGSYHQTAIAKKIM
EECCCCHHHHHHHHHHCCCCCEEEECCCCCEECCCCCCCCCCCHHCCCCHHHHHHHHHHH
TAMGVKPHVYVAAN
HHHCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8113174; 9579061; 9384377; 1898729; 2125017; 2105301; 8144469 [H]