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The map label for this gene is mcrA [H]

Identifier: 78356262

GI number: 78356262

Start: 1241709

End: 1242491

Strand: Direct

Name: mcrA [H]

Synonym: Dde_1215

Alternate gene names: 78356262

Gene position: 1241709-1242491 (Clockwise)

Preceding gene: 78356261

Following gene: 78356263

Centisome position: 33.29

GC content: 62.84

Gene sequence:

>783_bases
ATGGTGCGCCGCACCGCCGCAATCTTGCTGCGGTTCTGCGCGGCGCGCTTTTTGTCAGATGAACGACGCCCCGCGGCATT
ATACCGCGGGGCGTTTTTATGCATATGCCCCATGCGGCCGCCGCGGATGCATCACTGCCGGCCGTGGCCGTCAAATCCGG
AGAAGAATAGTATGAAACAGGCCATACTGCAGGCCATTGATGACTTTGCACGCGGGGTGCGCCCTGCGTTTTATAAAACG
CCCAGACGCTGGTATCTGGTACATCGGGGCAGCCTGTATCCGGCCAAGGCCATATGGGCCATGGCCACGGGACAAGGTAG
CGCGGGCAGCTTCAACACCCGTGACGCACGCGCCGGACTGGCAGAGGCCGGTTTTGCTGTGCGGGCTTTTGAACCGGAAT
CCGTGCAGTGGCCCGATGAAGATGTGCGCCTTGCCATCAGGCAGCTGCAGCAGGCTGCGGATACAGACCCCGGCGATGAT
GCGGCGGAAACTCTTTGCCCGCCTGGCATGACCGTGCGCGAACAGGCTGTCTTCCGGCGCAGTCCCGTGGTCGCTGCCAG
AGTGCTGCTGCGTGCCGGTGGCTTCTGCGAATGCTGCGGGGCCCCCGCACCGTTTGTCAGCCGTGCAGACAACCTGCCTT
ATCTTGAGGTGCATCACGTCCGGCCGCTGGCAGAGGGCGGCGCGGATACTCCGGCCAACTGCATGGCGCTGTGCCCCAAC
TGCCACAGGCAGTTTCATTACGGCATCAAGCCGCCGGTATGTGCATTATCATCGTCGCGCTGA

Upstream 100 bases:

>100_bases
ACTTTGTGCCCGAAGCTGAAGGCAGGCATATCGACGCGTGCATCGCCGAAGAGCGCAAGCATCTGAAGCAGCTTATGGCA
ATGCTGCGCAAATAGACGTC

Downstream 100 bases:

>100_bases
GGGAACGGCTTTGATGTAGTGCAATCTTAGCCGTACTGTCATTCTGTTGTCACCTTCCGGGGACATATCCTTGCCAGCCA
ATGCGGCAGTGCCGTTTTTT

Product: HNH nuclease

Products: NA

Alternate protein names: EcoKMcrA [H]

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MVRRTAAILLRFCAARFLSDERRPAALYRGAFLCICPMRPPRMHHCRPWPSNPEKNSMKQAILQAIDDFARGVRPAFYKT
PRRWYLVHRGSLYPAKAIWAMATGQGSAGSFNTRDARAGLAEAGFAVRAFEPESVQWPDEDVRLAIRQLQQAADTDPGDD
AAETLCPPGMTVREQAVFRRSPVVAARVLLRAGGFCECCGAPAPFVSRADNLPYLEVHHVRPLAEGGADTPANCMALCPN
CHRQFHYGIKPPVCALSSSR

Sequences:

>Translated_260_residues
MVRRTAAILLRFCAARFLSDERRPAALYRGAFLCICPMRPPRMHHCRPWPSNPEKNSMKQAILQAIDDFARGVRPAFYKT
PRRWYLVHRGSLYPAKAIWAMATGQGSAGSFNTRDARAGLAEAGFAVRAFEPESVQWPDEDVRLAIRQLQQAADTDPGDD
AAETLCPPGMTVREQAVFRRSPVVAARVLLRAGGFCECCGAPAPFVSRADNLPYLEVHHVRPLAEGGADTPANCMALCPN
CHRQFHYGIKPPVCALSSSR
>Mature_260_residues
MVRRTAAILLRFCAARFLSDERRPAALYRGAFLCICPMRPPRMHHCRPWPSNPEKNSMKQAILQAIDDFARGVRPAFYKT
PRRWYLVHRGSLYPAKAIWAMATGQGSAGSFNTRDARAGLAEAGFAVRAFEPESVQWPDEDVRLAIRQLQQAADTDPGDD
AAETLCPPGMTVREQAVFRRSPVVAARVLLRAGGFCECCGAPAPFVSRADNLPYLEVHHVRPLAEGGADTPANCMALCPN
CHRQFHYGIKPPVCALSSSR

Specific function: Restriction of 5-methyl and 5-hydroxymethylcytosines at the specific DNA sequence C(me)CGG [H]

COG id: COG1403

COG function: function code V; Restriction endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787406, Length=75, Percent_Identity=58.6666666666667, Blast_Score=97, Evalue=7e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002711
- InterPro:   IPR003615 [H]

Pfam domain/function: PF01844 HNH [H]

EC number: 3.1.21.- [C]

Molecular weight: Translated: 28732; Mature: 28732

Theoretical pI: Translated: 9.10; Mature: 9.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
7.3 %Cys+Met (Translated Protein)
4.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
7.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRRTAAILLRFCAARFLSDERRPAALYRGAFLCICPMRPPRMHHCRPWPSNPEKNSMKQ
CCHHHHHHHHHHHHHHHHCCCCCCHHHHCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHH
AILQAIDDFARGVRPAFYKTPRRWYLVHRGSLYPAKAIWAMATGQGSAGSFNTRDARAGL
HHHHHHHHHHHCCCHHHHHCCCEEEEEECCCCCCHHHHHEEECCCCCCCCCCCCHHHCCH
AEAGFAVRAFEPESVQWPDEDVRLAIRQLQQAADTDPGDDAAETLCPPGMTVREQAVFRR
HHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCHHHHHHHHH
SPVVAARVLLRAGGFCECCGAPAPFVSRADNLPYLEVHHVRPLAEGGADTPANCMALCPN
CHHHHHHHHHHCCCCHHHCCCCCHHHHHCCCCCEEEEECCCHHHCCCCCCCHHHHHHCHH
CHRQFHYGIKPPVCALSSSR
HHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MVRRTAAILLRFCAARFLSDERRPAALYRGAFLCICPMRPPRMHHCRPWPSNPEKNSMKQ
CCHHHHHHHHHHHHHHHHCCCCCCHHHHCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHH
AILQAIDDFARGVRPAFYKTPRRWYLVHRGSLYPAKAIWAMATGQGSAGSFNTRDARAGL
HHHHHHHHHHHCCCHHHHHCCCEEEEEECCCCCCHHHHHEEECCCCCCCCCCCCHHHCCH
AEAGFAVRAFEPESVQWPDEDVRLAIRQLQQAADTDPGDDAAETLCPPGMTVREQAVFRR
HHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCHHHHHHHHH
SPVVAARVLLRAGGFCECCGAPAPFVSRADNLPYLEVHHVRPLAEGGADTPANCMALCPN
CHHHHHHHHHHCCCCHHHCCCCCHHHHHCCCCCEEEEECCCHHHCCCCCCCHHHHHHCHH
CHRQFHYGIKPPVCALSSSR
HHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1938927; 8905232; 9278503 [H]