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The map label for this gene is trmD

Identifier: 78356143

GI number: 78356143

Start: 1125049

End: 1126326

Strand: Reverse

Name: trmD

Synonym: Dde_1096

Alternate gene names: 78356143

Gene position: 1126326-1125049 (Counterclockwise)

Preceding gene: 78356144

Following gene: 78356142

Centisome position: 30.19

GC content: 58.14

Gene sequence:

>1278_bases
ATGCGTTTCAACATCGTCACTCTGTTTCCGGAGTATTTCGATTCCCCGCTGGCGTGCGGCCTTATGGGCAAAGCACGAGA
ATCCGGTCTGGTGGAAGTCGGCTTTGTAAACCCGCGCGACCACACCACCGACAGGCACCGCACAGTGGACGACCGTCCGT
ATGGCGGCGGCCCCGGCATGGTGATGATGCCAGGCCCGCTGGCCGCGGCACTGCGCAGCATTCCCCGCCCCGGCAGAATA
CTGCTTATGGCGCCCAAGGGTCGTCCTTTCACGCAGGAACTGGCCCGCGAACTGGCAACCGGTGACAATCTGACAATTAT
CTGCGGCCGGTATGAAGGCATAGACGCCCGTCTGGAATCGGTATTTCCCATCGAGCCGGTATCCATGGGCGATTTTGTGC
TCAACGGGGGCGAAACTGCCGCCATGGCCGTTATGGAAGCCACCGGCAGACTGGTGCCCGGATACATGGGGCATGAAGAA
TCAGGCGAAGAAGAAAGCTTTTCCTCCGGCCTGCTGGAATATCCGCACTATACGCGGCCCGAAATTTTTGAAGGGCACGA
AGTGCCGGAGATTCTGCGCTCCGGTGACCACGGGCGCATTGCCGCATGGCGGCGCGAGCAGGCCCTGAAAACAACTTTGC
TCAACAGACCAGAAATTTTGCGCGATGCGCAAATAGATTATAACGATTTAAAAATACTACGAAAAACAGACAGAGAAAGA
CCGGGAAGAAATCTGTACTGCGCGCTGGTACATTATCCCGTCGTAAACAAAGAAAATAAATCTGTCGCCGTTTCTTTGAC
AAACCTCGATATTCACGATATAGGCCGCTGTTCGTGCACGTTTGGTCTGGGTGGTTACTACATCACCACTCCCATTGAAG
ACCAGCGTCGACTGCTTGACGGTCTGCTCCGGCACTGGACGGACGGACCCGGCGTAAGCGCCAACCCCGACAGGGGCACG
GCACTGCGTCTTGTCCGGGGTGTTTCCACGGTAGAGGAAGCCATCGGAGATATCGAGCAGCGCACAGGGCAACGGCCGAC
GGTCATCGCCACCAGCGCCCGCGGCGCAGGCGATACCGGATTCGAATCGGTCCGCCGCATGCTGCGTGAAAAACCAGTGC
TGCTTCTGTTCGGCACAGCTCACGGGCTGGCTCCGGATGTGCTGGAACGCTGCGACGGCATACTGCGGCCGATACGGTCA
CTTGATGGGTACAACCATCTATCGGTGCGTACAGCAGCCGCGATTATTGTGGACAGGGTGCTGGGCGACGCCCTGTAG

Upstream 100 bases:

>100_bases
CCGCCCGGCCTGCTGGAGATTTATCTTTCCGGCAGCTGATGCCGTGCGGCGCTGTCTGCGGACAGCCGGGCCGACGCGGC
ATCACAAAGGCAAAAAAGGC

Downstream 100 bases:

>100_bases
GGGCTGACCACGGCAGTGCCGAAATGTTTCAAACCAGGACGCAAACTCCCTATTGCGGATAAATCAGGTTCCAAGGGGGA
CACGAATATGAATATCATTA

Product: tRNA (Guanine37-N1) methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MRFNIVTLFPEYFDSPLACGLMGKARESGLVEVGFVNPRDHTTDRHRTVDDRPYGGGPGMVMMPGPLAAALRSIPRPGRI
LLMAPKGRPFTQELARELATGDNLTIICGRYEGIDARLESVFPIEPVSMGDFVLNGGETAAMAVMEATGRLVPGYMGHEE
SGEEESFSSGLLEYPHYTRPEIFEGHEVPEILRSGDHGRIAAWRREQALKTTLLNRPEILRDAQIDYNDLKILRKTDRER
PGRNLYCALVHYPVVNKENKSVAVSLTNLDIHDIGRCSCTFGLGGYYITTPIEDQRRLLDGLLRHWTDGPGVSANPDRGT
ALRLVRGVSTVEEAIGDIEQRTGQRPTVIATSARGAGDTGFESVRRMLREKPVLLLFGTAHGLAPDVLERCDGILRPIRS
LDGYNHLSVRTAAAIIVDRVLGDAL

Sequences:

>Translated_425_residues
MRFNIVTLFPEYFDSPLACGLMGKARESGLVEVGFVNPRDHTTDRHRTVDDRPYGGGPGMVMMPGPLAAALRSIPRPGRI
LLMAPKGRPFTQELARELATGDNLTIICGRYEGIDARLESVFPIEPVSMGDFVLNGGETAAMAVMEATGRLVPGYMGHEE
SGEEESFSSGLLEYPHYTRPEIFEGHEVPEILRSGDHGRIAAWRREQALKTTLLNRPEILRDAQIDYNDLKILRKTDRER
PGRNLYCALVHYPVVNKENKSVAVSLTNLDIHDIGRCSCTFGLGGYYITTPIEDQRRLLDGLLRHWTDGPGVSANPDRGT
ALRLVRGVSTVEEAIGDIEQRTGQRPTVIATSARGAGDTGFESVRRMLREKPVLLLFGTAHGLAPDVLERCDGILRPIRS
LDGYNHLSVRTAAAIIVDRVLGDAL
>Mature_425_residues
MRFNIVTLFPEYFDSPLACGLMGKARESGLVEVGFVNPRDHTTDRHRTVDDRPYGGGPGMVMMPGPLAAALRSIPRPGRI
LLMAPKGRPFTQELARELATGDNLTIICGRYEGIDARLESVFPIEPVSMGDFVLNGGETAAMAVMEATGRLVPGYMGHEE
SGEEESFSSGLLEYPHYTRPEIFEGHEVPEILRSGDHGRIAAWRREQALKTTLLNRPEILRDAQIDYNDLKILRKTDRER
PGRNLYCALVHYPVVNKENKSVAVSLTNLDIHDIGRCSCTFGLGGYYITTPIEDQRRLLDGLLRHWTDGPGVSANPDRGT
ALRLVRGVSTVEEAIGDIEQRTGQRPTVIATSARGAGDTGFESVRRMLREKPVLLLFGTAHGLAPDVLERCDGILRPIRS
LDGYNHLSVRTAAAIIVDRVLGDAL

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=243, Percent_Identity=45.2674897119342, Blast_Score=216, Evalue=3e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_DESDG (Q313J9)

Other databases:

- EMBL:   CP000112
- RefSeq:   YP_387592.1
- ProteinModelPortal:   Q313J9
- SMR:   Q313J9
- STRING:   Q313J9
- GeneID:   3755445
- GenomeReviews:   CP000112_GR
- KEGG:   dde:Dde_1096
- NMPDR:   fig|207559.3.peg.925
- eggNOG:   COG0336
- HOGENOM:   HBG457782
- OMA:   TIICGRY
- ProtClustDB:   CLSK704885
- BioCyc:   DDES207559:DDE_1096-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR019230
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF09936 DUF2168; PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 46820; Mature: 46820

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: NA

Important sites: BINDING 109-109

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFNIVTLFPEYFDSPLACGLMGKARESGLVEVGFVNPRDHTTDRHRTVDDRPYGGGPGM
CCEEEEEECHHHCCCCHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCE
VMMPGPLAAALRSIPRPGRILLMAPKGRPFTQELARELATGDNLTIICGRYEGIDARLES
EEECCHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCEEEEEECCCCCHHHHHH
VFPIEPVSMGDFVLNGGETAAMAVMEATGRLVPGYMGHEESGEEESFSSGLLEYPHYTRP
CCCCCCCCCCCEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCC
EIFEGHEVPEILRSGDHGRIAAWRREQALKTTLLNRPEILRDAQIDYNDLKILRKTDRER
CCCCCCCHHHHHHCCCCCCEEHHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHCCC
PGRNLYCALVHYPVVNKENKSVAVSLTNLDIHDIGRCSCTFGLGGYYITTPIEDQRRLLD
CCCCEEEEEEEECEECCCCCEEEEEEECCCHHHCCCEEEEECCCCEEEECCCHHHHHHHH
GLLRHWTDGPGVSANPDRGTALRLVRGVSTVEEAIGDIEQRTGQRPTVIATSARGAGDTG
HHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCC
FESVRRMLREKPVLLLFGTAHGLAPDVLERCDGILRPIRSLDGYNHLSVRTAAAIIVDRV
HHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
LGDAL
HHCCC
>Mature Secondary Structure
MRFNIVTLFPEYFDSPLACGLMGKARESGLVEVGFVNPRDHTTDRHRTVDDRPYGGGPGM
CCEEEEEECHHHCCCCHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCE
VMMPGPLAAALRSIPRPGRILLMAPKGRPFTQELARELATGDNLTIICGRYEGIDARLES
EEECCHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCEEEEEECCCCCHHHHHH
VFPIEPVSMGDFVLNGGETAAMAVMEATGRLVPGYMGHEESGEEESFSSGLLEYPHYTRP
CCCCCCCCCCCEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCC
EIFEGHEVPEILRSGDHGRIAAWRREQALKTTLLNRPEILRDAQIDYNDLKILRKTDRER
CCCCCCCHHHHHHCCCCCCEEHHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHCCC
PGRNLYCALVHYPVVNKENKSVAVSLTNLDIHDIGRCSCTFGLGGYYITTPIEDQRRLLD
CCCCEEEEEEEECEECCCCCEEEEEEECCCHHHCCCEEEEECCCCEEEECCCHHHHHHHH
GLLRHWTDGPGVSANPDRGTALRLVRGVSTVEEAIGDIEQRTGQRPTVIATSARGAGDTG
HHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCC
FESVRRMLREKPVLLLFGTAHGLAPDVLERCDGILRPIRSLDGYNHLSVRTAAAIIVDRV
HHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
LGDAL
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA