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The map label for this gene is pyk1 [H]

Identifier: 78356079

GI number: 78356079

Start: 1058438

End: 1059850

Strand: Reverse

Name: pyk1 [H]

Synonym: Dde_1032

Alternate gene names: 78356079

Gene position: 1059850-1058438 (Counterclockwise)

Preceding gene: 78356096

Following gene: 78356078

Centisome position: 28.41

GC content: 59.38

Gene sequence:

>1413_bases
ATGAGAACGAAAATAATCGCAACAGTGGGCCCTGCCTCTTCGGGCCGGGAAACCCTCTCACGGCTGATTCAGGCCGGAGT
ACGCATATTCCGGCTCAATTTTTCACATGGTGACGCCTCGCAGTTTGTTGATTTGATTGCTCTCATGCGACAGCTGGAGG
CGGAATACCAGACACCCATAACCCTGCTGCAGGATCTTTCCGGCCCTAAAATCCGTATAGGTGAACTGCCGGACGGCGCC
CTGACGCTGGCAAAGGACGACCGTATCCTGCTGGGCATTCCCGGCAAGGCGGCGGCAGACGGATTACCCTTCGTTCCGTT
CACGCACAAGACAATTCTTGACGGCATGGAACCGGGCGACAGGGTGGTGCTGAGCGACGGCGGACTGCAGTTCCATGTGG
AAAGCAAGGTCGGCCCCGATCTGTTCTGTCTGGTGGCAAACAACAGCGGTATCATAACCTCGCGCAAGGGCCTTGCCCTG
CCGGGCAAGCCTATCCACCTGGCCGCCCTGACCGAAAAAGACAAAAAAGATCTGCAGGACGGGCTGCGCATGGGCGTGGA
TGCCGTGGCGCTTTCGTTTGTGCAGACGCCCGAAGATGTGGCCGAAGCCAAGGCACTCATCAAGGCGGCAGGCCGCAACG
TGCCTGTAATTGCCAAACTGGAACGGCGCAACGCCGTGGACAGGCTGGAAGAAATTCTGGAAGTGACAGACATTGTCATG
GTGGCCCGCGGCGATCTGGGTGTGGAATGCCCCCTGCCCGAGCTGCCCGCCATGCAGAAGCGCATCATCCGCGCCTGCAA
CCGCAAGGCAAAACCGGTTATCGTTGCCACGCAGATGCTGCTTTCCATGGTCAACAGCCCCAGCCCCACCCGCGCCGAAA
CCACTGACGTGGCCAACGCGGTGCTGGACGGTGCCGACTGCGTGATGCTGTCGGAAGAAACGGCCATGGGCAACTTCCCT
GTGGAGACGGTTCAGTTCATGCGGCAGATAGTCCGCGCGGCCGAAGAGCTGCATCTGGAGCAGTCTTCTGTGCTGCCCCC
GCTTGATGAGCAGAAGACACCTGATTTTCTGGCCTATTCCGCATGTCTGCTGGCCGACAAGACCTGCGCGGGTGCCATCG
TGGCACACAGCATGTCCGGCAGCGCGGCACGGGTGCTATCCGCCCGCAGACCCCGTCAGACCATCCATGCGCTTACTCCC
GATGACGAAGCGCTGCGCTTCCTCAACTTTTCATGGGGCGTATGGCCCCACAAGGTTGATGAAACACTGCCGGGTCATCT
TGAGCGTGCCGAACTCTTTGTGGACAACGCGCCGCAGTTTGAAGACGGGGCCGAAGTGGTGATAACAGCAGGACAGCCCA
AGCCCGGTGAAAAACCCCGCGGCACCAACCTGGTAAAAATATACAGAAAATAG

Upstream 100 bases:

>100_bases
GGCGTGTGTCAATGAGTGCGCGTGATTTTTACAGAAAAGAGAACGGCAGAATGAATCAAGACGCTTGACAAGCGCTGGCA
ATTGAAAAAGAATCGGCCTT

Downstream 100 bases:

>100_bases
AACATGAGCGCGGTAAAATCGACCATACCTGACAATATTTCGGAAGAATATTATCAGATCAGTATCGAGATTCTGAACAG
CTTCCCGAAGTACAGGCCGC

Product: pyruvate kinase

Products: NA

Alternate protein names: PK 1 [H]

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MRTKIIATVGPASSGRETLSRLIQAGVRIFRLNFSHGDASQFVDLIALMRQLEAEYQTPITLLQDLSGPKIRIGELPDGA
LTLAKDDRILLGIPGKAAADGLPFVPFTHKTILDGMEPGDRVVLSDGGLQFHVESKVGPDLFCLVANNSGIITSRKGLAL
PGKPIHLAALTEKDKKDLQDGLRMGVDAVALSFVQTPEDVAEAKALIKAAGRNVPVIAKLERRNAVDRLEEILEVTDIVM
VARGDLGVECPLPELPAMQKRIIRACNRKAKPVIVATQMLLSMVNSPSPTRAETTDVANAVLDGADCVMLSEETAMGNFP
VETVQFMRQIVRAAEELHLEQSSVLPPLDEQKTPDFLAYSACLLADKTCAGAIVAHSMSGSAARVLSARRPRQTIHALTP
DDEALRFLNFSWGVWPHKVDETLPGHLERAELFVDNAPQFEDGAEVVITAGQPKPGEKPRGTNLVKIYRK

Sequences:

>Translated_470_residues
MRTKIIATVGPASSGRETLSRLIQAGVRIFRLNFSHGDASQFVDLIALMRQLEAEYQTPITLLQDLSGPKIRIGELPDGA
LTLAKDDRILLGIPGKAAADGLPFVPFTHKTILDGMEPGDRVVLSDGGLQFHVESKVGPDLFCLVANNSGIITSRKGLAL
PGKPIHLAALTEKDKKDLQDGLRMGVDAVALSFVQTPEDVAEAKALIKAAGRNVPVIAKLERRNAVDRLEEILEVTDIVM
VARGDLGVECPLPELPAMQKRIIRACNRKAKPVIVATQMLLSMVNSPSPTRAETTDVANAVLDGADCVMLSEETAMGNFP
VETVQFMRQIVRAAEELHLEQSSVLPPLDEQKTPDFLAYSACLLADKTCAGAIVAHSMSGSAARVLSARRPRQTIHALTP
DDEALRFLNFSWGVWPHKVDETLPGHLERAELFVDNAPQFEDGAEVVITAGQPKPGEKPRGTNLVKIYRK
>Mature_470_residues
MRTKIIATVGPASSGRETLSRLIQAGVRIFRLNFSHGDASQFVDLIALMRQLEAEYQTPITLLQDLSGPKIRIGELPDGA
LTLAKDDRILLGIPGKAAADGLPFVPFTHKTILDGMEPGDRVVLSDGGLQFHVESKVGPDLFCLVANNSGIITSRKGLAL
PGKPIHLAALTEKDKKDLQDGLRMGVDAVALSFVQTPEDVAEAKALIKAAGRNVPVIAKLERRNAVDRLEEILEVTDIVM
VARGDLGVECPLPELPAMQKRIIRACNRKAKPVIVATQMLLSMVNSPSPTRAETTDVANAVLDGADCVMLSEETAMGNFP
VETVQFMRQIVRAAEELHLEQSSVLPPLDEQKTPDFLAYSACLLADKTCAGAIVAHSMSGSAARVLSARRPRQTIHALTP
DDEALRFLNFSWGVWPHKVDETLPGHLERAELFVDNAPQFEDGAEVVITAGQPKPGEKPRGTNLVKIYRK

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI32967597, Length=429, Percent_Identity=40.7925407925408, Blast_Score=288, Evalue=6e-78,
Organism=Homo sapiens, GI10835121, Length=429, Percent_Identity=40.7925407925408, Blast_Score=288, Evalue=6e-78,
Organism=Homo sapiens, GI33286418, Length=436, Percent_Identity=37.8440366972477, Blast_Score=269, Evalue=4e-72,
Organism=Homo sapiens, GI33286422, Length=435, Percent_Identity=36.551724137931, Blast_Score=258, Evalue=7e-69,
Organism=Homo sapiens, GI33286420, Length=435, Percent_Identity=36.551724137931, Blast_Score=258, Evalue=7e-69,
Organism=Homo sapiens, GI310128732, Length=293, Percent_Identity=35.1535836177474, Blast_Score=166, Evalue=4e-41,
Organism=Homo sapiens, GI310128730, Length=293, Percent_Identity=35.1535836177474, Blast_Score=166, Evalue=4e-41,
Organism=Homo sapiens, GI310128736, Length=244, Percent_Identity=31.1475409836066, Blast_Score=119, Evalue=7e-27,
Organism=Homo sapiens, GI310128734, Length=244, Percent_Identity=31.1475409836066, Blast_Score=119, Evalue=7e-27,
Organism=Homo sapiens, GI310128738, Length=222, Percent_Identity=28.8288288288288, Blast_Score=98, Evalue=2e-20,
Organism=Escherichia coli, GI1787965, Length=421, Percent_Identity=37.29216152019, Blast_Score=258, Evalue=7e-70,
Organism=Escherichia coli, GI1788160, Length=430, Percent_Identity=36.7441860465116, Blast_Score=232, Evalue=5e-62,
Organism=Caenorhabditis elegans, GI17544584, Length=423, Percent_Identity=36.4066193853428, Blast_Score=257, Evalue=7e-69,
Organism=Caenorhabditis elegans, GI17506829, Length=425, Percent_Identity=37.8823529411765, Blast_Score=235, Evalue=4e-62,
Organism=Caenorhabditis elegans, GI71984413, Length=425, Percent_Identity=37.8823529411765, Blast_Score=234, Evalue=5e-62,
Organism=Caenorhabditis elegans, GI71984406, Length=425, Percent_Identity=37.8823529411765, Blast_Score=234, Evalue=6e-62,
Organism=Caenorhabditis elegans, GI17506831, Length=425, Percent_Identity=37.8823529411765, Blast_Score=234, Evalue=6e-62,
Organism=Saccharomyces cerevisiae, GI6319279, Length=433, Percent_Identity=39.4919168591224, Blast_Score=268, Evalue=2e-72,
Organism=Saccharomyces cerevisiae, GI6324923, Length=427, Percent_Identity=38.8758782201405, Blast_Score=254, Evalue=1e-68,
Organism=Drosophila melanogaster, GI24648964, Length=425, Percent_Identity=38.5882352941176, Blast_Score=256, Evalue=2e-68,
Organism=Drosophila melanogaster, GI28571814, Length=425, Percent_Identity=38.5882352941176, Blast_Score=256, Evalue=3e-68,
Organism=Drosophila melanogaster, GI24648966, Length=421, Percent_Identity=36.3420427553444, Blast_Score=221, Evalue=1e-57,
Organism=Drosophila melanogaster, GI24581235, Length=427, Percent_Identity=35.5971896955504, Blast_Score=218, Evalue=7e-57,
Organism=Drosophila melanogaster, GI24646914, Length=276, Percent_Identity=35.5072463768116, Blast_Score=142, Evalue=4e-34,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 50906; Mature: 50906

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTKIIATVGPASSGRETLSRLIQAGVRIFRLNFSHGDASQFVDLIALMRQLEAEYQTPI
CCCEEEEEECCCCCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCHH
TLLQDLSGPKIRIGELPDGALTLAKDDRILLGIPGKAAADGLPFVPFTHKTILDGMEPGD
HHHHCCCCCEEEECCCCCCCEEEECCCEEEEECCCCHHCCCCCCCCCCHHHHHCCCCCCC
RVVLSDGGLQFHVESKVGPDLFCLVANNSGIITSRKGLALPGKPIHLAALTEKDKKDLQD
EEEEECCCEEEEECCCCCCCEEEEEECCCCEEECCCCCCCCCCCEEEEEECCHHHHHHHH
GLRMGVDAVALSFVQTPEDVAEAKALIKAAGRNVPVIAKLERRNAVDRLEEILEVTDIVM
HHHHCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHE
VARGDLGVECPLPELPAMQKRIIRACNRKAKPVIVATQMLLSMVNSPSPTRAETTDVANA
EECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHH
VLDGADCVMLSEETAMGNFPVETVQFMRQIVRAAEELHLEQSSVLPPLDEQKTPDFLAYS
HHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
ACLLADKTCAGAIVAHSMSGSAARVLSARRPRQTIHALTPDDEALRFLNFSWGVWPHKVD
HHHHHCCCHHHHHHEECCCCCHHHHHHHCCCHHHHEECCCCHHHHHEEECCCCCCCCHHC
ETLPGHLERAELFVDNAPQFEDGAEVVITAGQPKPGEKPRGTNLVKIYRK
CCCCCCHHHHHHEECCCCCCCCCCCEEEECCCCCCCCCCCCCCEEEEEEC
>Mature Secondary Structure
MRTKIIATVGPASSGRETLSRLIQAGVRIFRLNFSHGDASQFVDLIALMRQLEAEYQTPI
CCCEEEEEECCCCCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCHH
TLLQDLSGPKIRIGELPDGALTLAKDDRILLGIPGKAAADGLPFVPFTHKTILDGMEPGD
HHHHCCCCCEEEECCCCCCCEEEECCCEEEEECCCCHHCCCCCCCCCCHHHHHCCCCCCC
RVVLSDGGLQFHVESKVGPDLFCLVANNSGIITSRKGLALPGKPIHLAALTEKDKKDLQD
EEEEECCCEEEEECCCCCCCEEEEEECCCCEEECCCCCCCCCCCEEEEEECCHHHHHHHH
GLRMGVDAVALSFVQTPEDVAEAKALIKAAGRNVPVIAKLERRNAVDRLEEILEVTDIVM
HHHHCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHE
VARGDLGVECPLPELPAMQKRIIRACNRKAKPVIVATQMLLSMVNSPSPTRAETTDVANA
EECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHH
VLDGADCVMLSEETAMGNFPVETVQFMRQIVRAAEELHLEQSSVLPPLDEQKTPDFLAYS
HHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
ACLLADKTCAGAIVAHSMSGSAARVLSARRPRQTIHALTPDDEALRFLNFSWGVWPHKVD
HHHHHCCCHHHHHHEECCCCCHHHHHHHCCCHHHHEECCCCHHHHHEEECCCCCCCCHHC
ETLPGHLERAELFVDNAPQFEDGAEVVITAGQPKPGEKPRGTNLVKIYRK
CCCCCCHHHHHHEECCCCCCCCCCCEEEECCCCCCCCCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]