Click here to switch to the map view.

The map label for this gene is trmJ [H]

Identifier: 78356021

GI number: 78356021

Start: 1002413

End: 1003174

Strand: Direct

Name: trmJ [H]

Synonym: Dde_0974

Alternate gene names: 78356021

Gene position: 1002413-1003174 (Clockwise)

Preceding gene: 78356020

Following gene: 78356024

Centisome position: 26.87

GC content: 57.22

Gene sequence:

>762_bases
ATGATGCTTGAAAATGTCGCCGTGGTGCTTGTGGAGCCCCGGTTTCCTGAAAATATAGGTATGGTGGCCCGCGCCTGCGC
CAATATGGGCGTAAACGAACTGGTGGTGGTGGCCCCCGAAAGGTGGAACCCCGAAAAATCTGTTCCGCTGGCAACGCCCA
AGGGGGTGCCGGTGCTGGAATCTGTGCGGCACGAAGATACGCTGGCCGCGGCGCTGGCACCATATACGCTGGTTCTGGGC
ACCACTGCCAGAACAGGCGGATGGCGGCGTCAGATATTGTCGCCTGAAACAGCCGCACCCGTGGTGCAGCAGGAAGTGGC
CGCAGGCGGCAGGGTGGCCATTGTTTTCGGACGTGAAGACAGAGGCCTGACCAACGAGGAGACGGAAGTCTGTTCGCGCC
TTGTGACCATTCCTACCAGCGAGGCAAGCTCCCTTAACCTTGCGCAGGCTGTGCTCATTCTGCTGTATGAATGTTTCAAG
CTGTCTGATGCCAGGCCTTTTCACAAATCGGCGGGGGCACTTTCGCGGGTTGTCACGCATGAAGAGCAGGAAATCCTGTT
CGCGGCATTGCAGGATACTCTGCTGGACATTGACTATCTGCGCGAGGATAATCCGGACTATTTCATGCTGCCCGTGCGAC
GTTTTATGCAGAAAGCTTCGCTGCGCAGACATGAATTTGATATGCTGATGGGCATATGCAGACAGGTCAGGCGGCTTGCC
CGTGTGGCAGCCGAAAAAGAAGACCGCGACGCATCAGCATAG

Upstream 100 bases:

>100_bases
CCGCCGGCAGGCACGTTGCGTCCCGGTTGTGAAACGCATATGAGTACGGCGCGGGCGGCAATGCGGCCCGCGCCTTTTTA
TTTTTGCCGGAGGATAGCGC

Downstream 100 bases:

>100_bases
GTTCCGCAGCGGCGGGACAGATTTTTTCCGGCTCCGGCACCATCGGTGCCGGATTTTTTGTATGTATTCTGAGCGTGCTT
CAGATTTTGCGGCCGGTGGC

Product: RNA methyltransferase

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MMLENVAVVLVEPRFPENIGMVARACANMGVNELVVVAPERWNPEKSVPLATPKGVPVLESVRHEDTLAAALAPYTLVLG
TTARTGGWRRQILSPETAAPVVQQEVAAGGRVAIVFGREDRGLTNEETEVCSRLVTIPTSEASSLNLAQAVLILLYECFK
LSDARPFHKSAGALSRVVTHEEQEILFAALQDTLLDIDYLREDNPDYFMLPVRRFMQKASLRRHEFDMLMGICRQVRRLA
RVAAEKEDRDASA

Sequences:

>Translated_253_residues
MMLENVAVVLVEPRFPENIGMVARACANMGVNELVVVAPERWNPEKSVPLATPKGVPVLESVRHEDTLAAALAPYTLVLG
TTARTGGWRRQILSPETAAPVVQQEVAAGGRVAIVFGREDRGLTNEETEVCSRLVTIPTSEASSLNLAQAVLILLYECFK
LSDARPFHKSAGALSRVVTHEEQEILFAALQDTLLDIDYLREDNPDYFMLPVRRFMQKASLRRHEFDMLMGICRQVRRLA
RVAAEKEDRDASA
>Mature_253_residues
MMLENVAVVLVEPRFPENIGMVARACANMGVNELVVVAPERWNPEKSVPLATPKGVPVLESVRHEDTLAAALAPYTLVLG
TTARTGGWRRQILSPETAAPVVQQEVAAGGRVAIVFGREDRGLTNEETEVCSRLVTIPTSEASSLNLAQAVLILLYECFK
LSDARPFHKSAGALSRVVTHEEQEILFAALQDTLLDIDYLREDNPDYFMLPVRRFMQKASLRRHEFDMLMGICRQVRRLA
RVAAEKEDRDASA

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=246, Percent_Identity=34.9593495934959, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1790865, Length=157, Percent_Identity=31.8471337579618, Blast_Score=80, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 28053; Mature: 28053

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMLENVAVVLVEPRFPENIGMVARACANMGVNELVVVAPERWNPEKSVPLATPKGVPVLE
CCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCHHHH
SVRHEDTLAAALAPYTLVLGTTARTGGWRRQILSPETAAPVVQQEVAAGGRVAIVFGRED
HHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHCCCCHHHHHHHHHHCCCCEEEEEECCCC
RGLTNEETEVCSRLVTIPTSEASSLNLAQAVLILLYECFKLSDARPFHKSAGALSRVVTH
CCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHC
EEQEILFAALQDTLLDIDYLREDNPDYFMLPVRRFMQKASLRRHEFDMLMGICRQVRRLA
CHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RVAAEKEDRDASA
HHHHCCCCCCCCC
>Mature Secondary Structure
MMLENVAVVLVEPRFPENIGMVARACANMGVNELVVVAPERWNPEKSVPLATPKGVPVLE
CCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCHHHH
SVRHEDTLAAALAPYTLVLGTTARTGGWRRQILSPETAAPVVQQEVAAGGRVAIVFGRED
HHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHCCCCHHHHHHHHHHCCCCEEEEEECCCC
RGLTNEETEVCSRLVTIPTSEASSLNLAQAVLILLYECFKLSDARPFHKSAGALSRVVTH
CCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHC
EEQEILFAALQDTLLDIDYLREDNPDYFMLPVRRFMQKASLRRHEFDMLMGICRQVRRLA
CHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RVAAEKEDRDASA
HHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA