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The map label for this gene is fdhB [H]

Identifier: 78355859

GI number: 78355859

Start: 832336

End: 833064

Strand: Reverse

Name: fdhB [H]

Synonym: Dde_0812

Alternate gene names: 78355859

Gene position: 833064-832336 (Counterclockwise)

Preceding gene: 78355860

Following gene: 78355858

Centisome position: 22.33

GC content: 57.75

Gene sequence:

>729_bases
ATGCAAAAAAGCTTTCTTATAGACATGTCACGCTGCACCGCCTGCCGCGGCTGCCAGATGGCCTGCAAGCAATGGCATAA
GTTTCCCGGCACCAGAACAGTGCAGCGGGGCTCTTTCCAGAATCCGGAAGACCTTTCAACCACCACCTACAAACTTGTCC
GCTTCAGCGAAGCACAGGTGAACGGCAAGCTGGCATGGCTGTTTTTTCCCGAACAGTGCCGCCACTGTATCGTTCCGCCG
TGTAAAGACGTGGCCGACGCCTATGTGGAAGGGGCCATACTGCGCGACCCCGACACGCAGGCGGTGCTGTATACCGACCT
GACGCGCAGACTCACCCCCGAACAAAAAGCGGACGTGCATTCCGCCTGCCCGTACAACATTCCCCGCTGGGACGATGAAA
CAGGCATCATGGCCAAATGCGACATGTGCTATGACAGAGTAATCAACGGCCTGAAACCGGCCTGTGTGCAGACCTGTCCC
ACCGGCACCATGAACTTCGGCAACAGGGACGAGATGCTTGAACTGGCACAGCAGCGGCTTGAGGCAGCCAAAAAGAAATA
CCCCGCCGCACGGCTGGTCGACCCGGACTACACCAATGTAATCTTCCTCGCCGTGGACGACCCCAAGCTCTATTACGAGT
ATCTGGAAGCGGATGCCTCCGGCGTACTGCCCATGGACAGACGGCACTTTTTTGCCATGCTGCGCCCCGGCGTACGCAGA
CAGGGATAA

Upstream 100 bases:

>100_bases
CTCCAACCTGCTTACAGCGGCCGTGGGCGACGCCAATACCGGCATTCCCGAAACCAAGGTCTTCATGGCCAATGTGCGCA
AGGCGTAAAGGAGTTACAGC

Downstream 100 bases:

>100_bases
CATCCGGCGGTGCAACCGCAGTTGCAGGGGACAGGCGGAAAACGCCTGTCCCCTCTTCTGTCTGAAGTACCGTACCGTTC
CGCCCGTGTCGCGCGGCTTG

Product: formate dehydrogenase subunit beta

Products: CO2; NADH2 [C]

Alternate protein names: FDH subunit beta; Formate dehydrogenase small subunit [H]

Number of amino acids: Translated: 242; Mature: 242

Protein sequence:

>242_residues
MQKSFLIDMSRCTACRGCQMACKQWHKFPGTRTVQRGSFQNPEDLSTTTYKLVRFSEAQVNGKLAWLFFPEQCRHCIVPP
CKDVADAYVEGAILRDPDTQAVLYTDLTRRLTPEQKADVHSACPYNIPRWDDETGIMAKCDMCYDRVINGLKPACVQTCP
TGTMNFGNRDEMLELAQQRLEAAKKKYPAARLVDPDYTNVIFLAVDDPKLYYEYLEADASGVLPMDRRHFFAMLRPGVRR
QG

Sequences:

>Translated_242_residues
MQKSFLIDMSRCTACRGCQMACKQWHKFPGTRTVQRGSFQNPEDLSTTTYKLVRFSEAQVNGKLAWLFFPEQCRHCIVPP
CKDVADAYVEGAILRDPDTQAVLYTDLTRRLTPEQKADVHSACPYNIPRWDDETGIMAKCDMCYDRVINGLKPACVQTCP
TGTMNFGNRDEMLELAQQRLEAAKKKYPAARLVDPDYTNVIFLAVDDPKLYYEYLEADASGVLPMDRRHFFAMLRPGVRR
QG
>Mature_242_residues
MQKSFLIDMSRCTACRGCQMACKQWHKFPGTRTVQRGSFQNPEDLSTTTYKLVRFSEAQVNGKLAWLFFPEQCRHCIVPP
CKDVADAYVEGAILRDPDTQAVLYTDLTRRLTPEQKADVHSACPYNIPRWDDETGIMAKCDMCYDRVINGLKPACVQTCP
TGTMNFGNRDEMLELAQQRLEAAKKKYPAARLVDPDYTNVIFLAVDDPKLYYEYLEADASGVLPMDRRHFFAMLRPGVRR
QG

Specific function: Beta chain of the formate dehydrogenase (FDH) catalyze the reversible two-electron oxidation of formate to carbon dioxide. FDH loses activity in the presence of air, but this activity can be restored. This chain is an electron transfer unit [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787749, Length=216, Percent_Identity=37.037037037037, Blast_Score=140, Evalue=6e-35,
Organism=Escherichia coli, GI1790326, Length=216, Percent_Identity=36.5740740740741, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1787122, Length=199, Percent_Identity=30.6532663316583, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI1787872, Length=199, Percent_Identity=29.6482412060301, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1789370, Length=240, Percent_Identity=27.9166666666667, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI2367345, Length=197, Percent_Identity=28.9340101522843, Blast_Score=61, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 27639; Mature: 27639

Theoretical pI: Translated: 7.80; Mature: 7.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
8.7 %Cys+Met (Translated Protein)
5.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
8.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKSFLIDMSRCTACRGCQMACKQWHKFPGTRTVQRGSFQNPEDLSTTTYKLVRFSEAQV
CCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHEECCCCC
NGKLAWLFFPEQCRHCIVPPCKDVADAYVEGAILRDPDTQAVLYTDLTRRLTPEQKADVH
CCEEEEEECHHHCCCCCCCCHHHHHHHHHCCCEEECCCCCEEEEEHHHHHCCCHHHCCCC
SACPYNIPRWDDETGIMAKCDMCYDRVINGLKPACVQTCPTGTMNFGNRDEMLELAQQRL
CCCCCCCCCCCCCCCCEEHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHH
EAAKKKYPAARLVDPDYTNVIFLAVDDPKLYYEYLEADASGVLPMDRRHFFAMLRPGVRR
HHHHHHCCCCCCCCCCCCCEEEEEECCHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCC
QG
CC
>Mature Secondary Structure
MQKSFLIDMSRCTACRGCQMACKQWHKFPGTRTVQRGSFQNPEDLSTTTYKLVRFSEAQV
CCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHEECCCCC
NGKLAWLFFPEQCRHCIVPPCKDVADAYVEGAILRDPDTQAVLYTDLTRRLTPEQKADVH
CCEEEEEECHHHCCCCCCCCHHHHHHHHHCCCEEECCCCCEEEEEHHHHHCCCHHHCCCC
SACPYNIPRWDDETGIMAKCDMCYDRVINGLKPACVQTCPTGTMNFGNRDEMLELAQQRL
CCCCCCCCCCCCCCCCEEHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHH
EAAKKKYPAARLVDPDYTNVIFLAVDDPKLYYEYLEADASGVLPMDRRHFFAMLRPGVRR
HHHHHHCCCCCCCCCCCCCEEEEEECCHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCC
QG
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD [C]

Specific reaction: formate + NAD = CO2 + NADH2 [C]

General reaction: Oxidoreductases; Acting on the aldehyde or oxo group of donors [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA