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The map label for this gene is livK [C]

Identifier: 78355759

GI number: 78355759

Start: 730242

End: 731684

Strand: Reverse

Name: livK [C]

Synonym: Dde_0712

Alternate gene names: 78355759

Gene position: 731684-730242 (Counterclockwise)

Preceding gene: 78355767

Following gene: 78355758

Centisome position: 19.61

GC content: 62.23

Gene sequence:

>1443_bases
GTGCCCGCCCCTGACCGCCGGTGTGGCACATATGCCGCAGACGCTGCCGTCAGCAGATGCCGCGCCCGCCAACGCCATAC
TTTCCGCGGAGGTCCCGTGACGTTTTTTCCGGCCCTGTCCTCTGTATCCCGCCCGCACAACACCCGCGTCCGCGGCGCAT
TATCCTTTGTACTGACTGTGCTGCTGGTGCTGCTGCCTGCGGCAGGCTGCGACCGCACGCGGGATACCGCGCAAGAAAGC
CGCTCCCGCACAGGCATCTCTGACACGGAAATACGGTTCGGCTCATCGCTGCCCCTTACCGGCCATGCCAGCTATCTGGG
CATACAGACCATGCGCGGCGCACTCAGCTATATTAAATACATAAATGAAAACGGTGGCATACACGGGCGACGCATTGTTC
TTGATGTACGGGACGACGGGTACGACCCGCCCCGCTGTCTTGCCAACACCCAGAACCTGCTCATAGAAGGCGATGTGTTC
GGCCTGTTCTGCTATGTGGGCACCCCCACCACCGTGCGTGTGCTGCCGCTTATCGAAGAGGCCCGCATCCCGCTGCTGGG
CATGTTCACAGGAGCCAACAGACTGCGCCATCCGTTCAGCCGCTATGTCATCAATGTGCGCGCCTCGTATTATCAGGAAA
CGGAAGCCGCGGTGCGGCATCTGGTACAGGATCTCGGCTTTACGCGCATAGCCGTGTTCTACCAGTACGACGATTACGGT
TTTGACGGACTTACGGGTACGGAACTGGCCCTGCGTGAGCTGGGGCTTGCGCCGGTGGCACGCGGATCATACCAGCGGGG
CACACTGGATGTGAAAGAAGGACTGAACCGCATACTGGCTGCTGATCCTCAGGCAGTTGTCATGGTGGGAACCTACGAGC
CGTGCGCCCGTTTCATCCGCATGGCCGCAGATGCCGGCATGCGCGCCGTGTTCCACACGCTTTCATTTGCCGGTGCCGAA
GAACTGGCCCGCCTGCTGCACGGCGACCACCGCAATCCCGTGACCATGTCACAGGTCGTCCCTCCCCCGTCCGCCCCGCA
GACACCGGAACTCATGGCCGACGCACGGCAATACACCGAACTCATGCGCCGCTATTATCCGCAGGACACGCCCTCCATCA
TCGGGCTGGAAGGATATGTCAACGCGCGCGTGCTGGTTGAAGCGCTGCGCCTTGCCGGACGCGGACTGACGCGCGAATCG
TTCATCGACGCCGTGGAATCGCTGCATGCGTTTCCGCTGGGCGGACGCAGCCGCGTAACATTCAGCAATACGGACCATCA
GGGCATGGATCAGGTTTACTTCACCCTGCTGCGCAACGGTGAGTTTGTGCTCATCAAGGACTTTGCTCCGGTAAGGGACC
TGCTGCCCCCTGCCGGCGGGCGGCCGGCCGCATCGGCACCGGACACGGCCCGGCAGCACAATGCCCGCGGAGCACAGTCA
TGA

Upstream 100 bases:

>100_bases
ATCAGGTGTCTGCATTGTACCAAACACCCGACGCCGCAGCCGCAGCAAGCGGCCCCTTGCCCTGAAGCCGCAAGGCGGGG
TAAGTCTGTCCGCATACGCA

Downstream 100 bases:

>100_bases
ACCGCCTGCTGGAAAAATTTTCGGCGCTGAGCCTGCAGAACAAATTCTTTTTTTCCATTCTCGCGGTCATCATGATCATC
AGCGGCACCATTGCGCTGCT

Product: putative lipoprotein

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: None

Number of amino acids: Translated: 480; Mature: 479

Protein sequence:

>480_residues
MPAPDRRCGTYAADAAVSRCRARQRHTFRGGPVTFFPALSSVSRPHNTRVRGALSFVLTVLLVLLPAAGCDRTRDTAQES
RSRTGISDTEIRFGSSLPLTGHASYLGIQTMRGALSYIKYINENGGIHGRRIVLDVRDDGYDPPRCLANTQNLLIEGDVF
GLFCYVGTPTTVRVLPLIEEARIPLLGMFTGANRLRHPFSRYVINVRASYYQETEAAVRHLVQDLGFTRIAVFYQYDDYG
FDGLTGTELALRELGLAPVARGSYQRGTLDVKEGLNRILAADPQAVVMVGTYEPCARFIRMAADAGMRAVFHTLSFAGAE
ELARLLHGDHRNPVTMSQVVPPPSAPQTPELMADARQYTELMRRYYPQDTPSIIGLEGYVNARVLVEALRLAGRGLTRES
FIDAVESLHAFPLGGRSRVTFSNTDHQGMDQVYFTLLRNGEFVLIKDFAPVRDLLPPAGGRPAASAPDTARQHNARGAQS

Sequences:

>Translated_480_residues
MPAPDRRCGTYAADAAVSRCRARQRHTFRGGPVTFFPALSSVSRPHNTRVRGALSFVLTVLLVLLPAAGCDRTRDTAQES
RSRTGISDTEIRFGSSLPLTGHASYLGIQTMRGALSYIKYINENGGIHGRRIVLDVRDDGYDPPRCLANTQNLLIEGDVF
GLFCYVGTPTTVRVLPLIEEARIPLLGMFTGANRLRHPFSRYVINVRASYYQETEAAVRHLVQDLGFTRIAVFYQYDDYG
FDGLTGTELALRELGLAPVARGSYQRGTLDVKEGLNRILAADPQAVVMVGTYEPCARFIRMAADAGMRAVFHTLSFAGAE
ELARLLHGDHRNPVTMSQVVPPPSAPQTPELMADARQYTELMRRYYPQDTPSIIGLEGYVNARVLVEALRLAGRGLTRES
FIDAVESLHAFPLGGRSRVTFSNTDHQGMDQVYFTLLRNGEFVLIKDFAPVRDLLPPAGGRPAASAPDTARQHNARGAQS
>Mature_479_residues
PAPDRRCGTYAADAAVSRCRARQRHTFRGGPVTFFPALSSVSRPHNTRVRGALSFVLTVLLVLLPAAGCDRTRDTAQESR
SRTGISDTEIRFGSSLPLTGHASYLGIQTMRGALSYIKYINENGGIHGRRIVLDVRDDGYDPPRCLANTQNLLIEGDVFG
LFCYVGTPTTVRVLPLIEEARIPLLGMFTGANRLRHPFSRYVINVRASYYQETEAAVRHLVQDLGFTRIAVFYQYDDYGF
DGLTGTELALRELGLAPVARGSYQRGTLDVKEGLNRILAADPQAVVMVGTYEPCARFIRMAADAGMRAVFHTLSFAGAEE
LARLLHGDHRNPVTMSQVVPPPSAPQTPELMADARQYTELMRRYYPQDTPSIIGLEGYVNARVLVEALRLAGRGLTRESF
IDAVESLHAFPLGGRSRVTFSNTDHQGMDQVYFTLLRNGEFVLIKDFAPVRDLLPPAGGRPAASAPDTARQHNARGAQS

Specific function: This Protein Is A Component Of The Leucine-Specific Transport System, Which Is One Of The Two Periplasmic Binding Protein-Dependent Transport Systems Of The High-Affinity Transport Of The Branched-Chain Amino Acids In E.Coli. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 52829; Mature: 52698

Theoretical pI: Translated: 9.05; Mature: 9.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAPDRRCGTYAADAAVSRCRARQRHTFRGGPVTFFPALSSVSRPHNTRVRGALSFVLTV
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHCCCCCCHHHHHHHHHHHHH
LLVLLPAAGCDRTRDTAQESRSRTGISDTEIRFGSSLPLTGHASYLGIQTMRGALSYIKY
HHHHHCCCCCCCCHHHHHHHHHCCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHH
INENGGIHGRRIVLDVRDDGYDPPRCLANTQNLLIEGDVFGLFCYVGTPTTVRVLPLIEE
HHCCCCCCCEEEEEEECCCCCCCHHHHCCCCCEEEECCEEEEEEEECCCCEEEEEEHHHH
ARIPLLGMFTGANRLRHPFSRYVINVRASYYQETEAAVRHLVQDLGFTRIAVFYQYDDYG
CCCCEEEEECCCHHHHCHHHHHEEHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCC
FDGLTGTELALRELGLAPVARGSYQRGTLDVKEGLNRILAADPQAVVMVGTYEPCARFIR
CCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEECHHHHHHHHH
MAADAGMRAVFHTLSFAGAEELARLLHGDHRNPVTMSQVVPPPSAPQTPELMADARQYTE
HHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCEEHHHCCCCCCCCCCHHHHHHHHHHHH
LMRRYYPQDTPSIIGLEGYVNARVLVEALRLAGRGLTRESFIDAVESLHAFPLGGRSRVT
HHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCEEE
FSNTDHQGMDQVYFTLLRNGEFVLIKDFAPVRDLLPPAGGRPAASAPDTARQHNARGAQS
ECCCCCCHHHHHHHHHHCCCCEEEEECCCCHHHHCCCCCCCCCCCCCCHHHHHCCCCCCC
>Mature Secondary Structure 
PAPDRRCGTYAADAAVSRCRARQRHTFRGGPVTFFPALSSVSRPHNTRVRGALSFVLTV
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHCCCCCCHHHHHHHHHHHHH
LLVLLPAAGCDRTRDTAQESRSRTGISDTEIRFGSSLPLTGHASYLGIQTMRGALSYIKY
HHHHHCCCCCCCCHHHHHHHHHCCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHH
INENGGIHGRRIVLDVRDDGYDPPRCLANTQNLLIEGDVFGLFCYVGTPTTVRVLPLIEE
HHCCCCCCCEEEEEEECCCCCCCHHHHCCCCCEEEECCEEEEEEEECCCCEEEEEEHHHH
ARIPLLGMFTGANRLRHPFSRYVINVRASYYQETEAAVRHLVQDLGFTRIAVFYQYDDYG
CCCCEEEEECCCHHHHCHHHHHEEHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCC
FDGLTGTELALRELGLAPVARGSYQRGTLDVKEGLNRILAADPQAVVMVGTYEPCARFIR
CCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEECHHHHHHHHH
MAADAGMRAVFHTLSFAGAEELARLLHGDHRNPVTMSQVVPPPSAPQTPELMADARQYTE
HHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCEEHHHCCCCCCCCCCHHHHHHHHHHHH
LMRRYYPQDTPSIIGLEGYVNARVLVEALRLAGRGLTRESFIDAVESLHAFPLGGRSRVT
HHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCEEE
FSNTDHQGMDQVYFTLLRNGEFVLIKDFAPVRDLLPPAGGRPAASAPDTARQHNARGAQS
ECCCCCCHHHHHHHHHHCCCCEEEEECCCCHHHHCCCCCCCCCCCCCCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA