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The map label for this gene is mdtE [H]

Identifier: 78355718

GI number: 78355718

Start: 687937

End: 689235

Strand: Reverse

Name: mdtE [H]

Synonym: Dde_0671

Alternate gene names: 78355718

Gene position: 689235-687937 (Counterclockwise)

Preceding gene: 78355723

Following gene: 78355717

Centisome position: 18.48

GC content: 57.81

Gene sequence:

>1299_bases
ATGAATAGCGGGTTGCGGCACATTCCGGAACCGCGTTGCGCCAGCCCGGCAGACACCGCCCCGCCGTGTTGTGCAGGCCC
GTTGTGCTATGTTATATCGTCTTATTGTTGCTGCCTGTTTCCATGTTTTTTTCCCAAAGGAGTTTACCCCATGACTCTGA
CCCGACGCTCTGCGTTTTTTCATCTGCCTGTGTTCACAGCCGTCATCCTGTGTATGGTAATGCTTACCGGCTGTGCCGAA
GAACCGCAGGAACAGGGGCCGCCACCTGTCAAGCCGGTAAAGGTGCTTACAGTGGGCAGTAAAGACAAAGGGGTCAGGCG
GGTGTTTCCCGGCAAGGTTGTTGCCGGTGAAAAGGTGGAGCTCGGCTTCAGAGTGGCCGGACAGCTGACCCGCTTTCCCG
TCAAGGAAAGCCAGCATGTGGAACAGGGCCAGACCGTGGCCGAACTGGACAAAAGCGACTTCATCACCAAAGTGCGCAAC
ATAGAATCGCAGCTGGGAGGGGCCAGAGCATCGCTCAATGAGGCCACGCTCAACTTTAAGCGCATGGAAACGCTGCTCGG
ACAGGACACCATATCAAAGGCGGACTACGACAAGGCACGGGCCTCGATGGACAATGCCAACGCCAAGGTGCTTTCGCTGA
CCCAGCAGCTCAAGCAGGCCACGCAGGATCTGGAATACACAACCCTGCGGGCGCCTTTTTCCGGCGTCATCGCCAAAAAA
TACGTAAAGAATTACGAGCTGGTGCAGGCCCAGCAACCCGTTCTGAAGCTCGAAAACACCGACCGGCTTGATGTGGAGGT
GGAAGTCCCCGAATTTGTCATTGCCCAGTTGCGGCATCAGGACAGAAAAAACATGCCTGCGCCTGTCGCCCGCTTTTCTG
CCTTTCCTGGGCGGTCTTTCGAGCTGAGCCTCAAAGAATACCAGACATCTGCCAATCCGCAGACCCAGACCTATACCGTC
ACACTGACCATGCAGAGCCCCGACGACATACGGCTGCTGCCGGGCATGACCGCCGACGTGGAGGGCACCCTGCCCTTCGG
CGGCGACATCGATGCCACATCGCTGCCGGTTGCCGCCGTGGTGGGCGGCGAAGACGACCGCACCTACATATGGGTGCTGG
ACAAAGAAAGCATGACAGTATCACGCCGCGCCGTGGTTACAGGGGCCATACATGAAGACAAGGTGGCCATCACTCAGGGC
CTTGAACCCGGCATGACGGTTGTAGCCGCCGGAGCAAACTATCTGCACGAAGGACAGAAAGTACGCATCCTCAACGGCAA
GATCGGGGGCGGGCAATGA

Upstream 100 bases:

>100_bases
ATTGACAATATTACATATCATAATGAGAATGTCCACCCTCTGTTTTTTCCGCACTGAATCATGGCTTATTATAATCATCG
TCAGTCCGCTGTGATTGTGC

Downstream 100 bases:

>100_bases
ACATTGCGGAATTCTGCATCGGCAAGCGCACGGTAAGTCTTGTACTTACGCTGGCGCTGACCGTGGGCGGCATACTGGCC
TATATGAAGATGGGCAGGCT

Product: secretion protein HlyD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MNSGLRHIPEPRCASPADTAPPCCAGPLCYVISSYCCCLFPCFFPKGVYPMTLTRRSAFFHLPVFTAVILCMVMLTGCAE
EPQEQGPPPVKPVKVLTVGSKDKGVRRVFPGKVVAGEKVELGFRVAGQLTRFPVKESQHVEQGQTVAELDKSDFITKVRN
IESQLGGARASLNEATLNFKRMETLLGQDTISKADYDKARASMDNANAKVLSLTQQLKQATQDLEYTTLRAPFSGVIAKK
YVKNYELVQAQQPVLKLENTDRLDVEVEVPEFVIAQLRHQDRKNMPAPVARFSAFPGRSFELSLKEYQTSANPQTQTYTV
TLTMQSPDDIRLLPGMTADVEGTLPFGGDIDATSLPVAAVVGGEDDRTYIWVLDKESMTVSRRAVVTGAIHEDKVAITQG
LEPGMTVVAAGANYLHEGQKVRILNGKIGGGQ

Sequences:

>Translated_432_residues
MNSGLRHIPEPRCASPADTAPPCCAGPLCYVISSYCCCLFPCFFPKGVYPMTLTRRSAFFHLPVFTAVILCMVMLTGCAE
EPQEQGPPPVKPVKVLTVGSKDKGVRRVFPGKVVAGEKVELGFRVAGQLTRFPVKESQHVEQGQTVAELDKSDFITKVRN
IESQLGGARASLNEATLNFKRMETLLGQDTISKADYDKARASMDNANAKVLSLTQQLKQATQDLEYTTLRAPFSGVIAKK
YVKNYELVQAQQPVLKLENTDRLDVEVEVPEFVIAQLRHQDRKNMPAPVARFSAFPGRSFELSLKEYQTSANPQTQTYTV
TLTMQSPDDIRLLPGMTADVEGTLPFGGDIDATSLPVAAVVGGEDDRTYIWVLDKESMTVSRRAVVTGAIHEDKVAITQG
LEPGMTVVAAGANYLHEGQKVRILNGKIGGGQ
>Mature_432_residues
MNSGLRHIPEPRCASPADTAPPCCAGPLCYVISSYCCCLFPCFFPKGVYPMTLTRRSAFFHLPVFTAVILCMVMLTGCAE
EPQEQGPPPVKPVKVLTVGSKDKGVRRVFPGKVVAGEKVELGFRVAGQLTRFPVKESQHVEQGQTVAELDKSDFITKVRN
IESQLGGARASLNEATLNFKRMETLLGQDTISKADYDKARASMDNANAKVLSLTQQLKQATQDLEYTTLRAPFSGVIAKK
YVKNYELVQAQQPVLKLENTDRLDVEVEVPEFVIAQLRHQDRKNMPAPVARFSAFPGRSFELSLKEYQTSANPQTQTYTV
TLTMQSPDDIRLLPGMTADVEGTLPFGGDIDATSLPVAAVVGGEDDRTYIWVLDKESMTVSRRAVVTGAIHEDKVAITQG
LEPGMTVVAAGANYLHEGQKVRILNGKIGGGQ

Specific function: Part of a multidrug resistance efflux system that confers resistance to compounds such as rhodamine 6G, erythromycin, doxorubicin, ethidium bromide, TPP, SDS, deoxycholate, crystal violet and benzalkonium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789929, Length=372, Percent_Identity=26.0752688172043, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI87082036, Length=344, Percent_Identity=24.4186046511628, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1786668, Length=369, Percent_Identity=27.10027100271, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1789665, Length=381, Percent_Identity=24.9343832020997, Blast_Score=76, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 47184; Mature: 47184

Theoretical pI: Translated: 7.65; Mature: 7.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSGLRHIPEPRCASPADTAPPCCAGPLCYVISSYCCCLFPCFFPKGVYPMTLTRRSAFF
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEE
HLPVFTAVILCMVMLTGCAEEPQEQGPPPVKPVKVLTVGSKDKGVRRVFPGKVVAGEKVE
ECHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCEEEEEECCCCCCCEEECCCCEECCCEEE
LGFRVAGQLTRFPVKESQHVEQGQTVAELDKSDFITKVRNIESQLGGARASLNEATLNFK
ECEEECCHHHCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHCHHHHHHHHH
RMETLLGQDTISKADYDKARASMDNANAKVLSLTQQLKQATQDLEYTTLRAPFSGVIAKK
HHHHHHCHHHHCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHH
YVKNYELVQAQQPVLKLENTDRLDVEVEVPEFVIAQLRHQDRKNMPAPVARFSAFPGRSF
HHHCHHHHHHCCCCEEECCCCCEEEEEECHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCE
ELSLKEYQTSANPQTQTYTVTLTMQSPDDIRLLPGMTADVEGTLPFGGDIDATSLPVAAV
EEEHHHHHCCCCCCCEEEEEEEEECCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCEEEE
VGGEDDRTYIWVLDKESMTVSRRAVVTGAIHEDKVAITQGLEPGMTVVAAGANYLHEGQK
ECCCCCCEEEEEECCCCCEEHHHEEEEECCCCCCCHHHCCCCCCCEEEECCCHHHHCCCE
VRILNGKIGGGQ
EEEEECCCCCCC
>Mature Secondary Structure
MNSGLRHIPEPRCASPADTAPPCCAGPLCYVISSYCCCLFPCFFPKGVYPMTLTRRSAFF
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEE
HLPVFTAVILCMVMLTGCAEEPQEQGPPPVKPVKVLTVGSKDKGVRRVFPGKVVAGEKVE
ECHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCEEEEEECCCCCCCEEECCCCEECCCEEE
LGFRVAGQLTRFPVKESQHVEQGQTVAELDKSDFITKVRNIESQLGGARASLNEATLNFK
ECEEECCHHHCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHCHHHHHHHHH
RMETLLGQDTISKADYDKARASMDNANAKVLSLTQQLKQATQDLEYTTLRAPFSGVIAKK
HHHHHHCHHHHCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHH
YVKNYELVQAQQPVLKLENTDRLDVEVEVPEFVIAQLRHQDRKNMPAPVARFSAFPGRSF
HHHCHHHHHHCCCCEEECCCCCEEEEEECHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCE
ELSLKEYQTSANPQTQTYTVTLTMQSPDDIRLLPGMTADVEGTLPFGGDIDATSLPVAAV
EEEHHHHHCCCCCCCEEEEEEEEECCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCEEEE
VGGEDDRTYIWVLDKESMTVSRRAVVTGAIHEDKVAITQGLEPGMTVVAAGANYLHEGQK
ECCCCCCEEEEEECCCCCEEHHHEEEEECCCCCCCHHHCCCCCCCEEEECCCHHHHCCCE
VRILNGKIGGGQ
EEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]