| Definition | Desulfovibrio desulfuricans subsp. desulfuricans str. G20 chromosome, complete genome. |
|---|---|
| Accession | NC_007519 |
| Length | 3,730,232 |
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The map label for this gene is leuA2 [H]
Identifier: 78355567
GI number: 78355567
Start: 527874
End: 529688
Strand: Reverse
Name: leuA2 [H]
Synonym: Dde_0520
Alternate gene names: 78355567
Gene position: 529688-527874 (Counterclockwise)
Preceding gene: 78355568
Following gene: 78355566
Centisome position: 14.2
GC content: 55.7
Gene sequence:
>1815_bases ATGAGCCTTTTGCACCTGAACCGCCCGGTAACCCGGCTGTCGCTGCAGAACAGAGAAGATCCCGAACTGTACCGCGACAT ATTTCCCTATTCCAAAATCAGCAGAATCGCCTTTGACGATATCCACATCATGCCGCGCCCTGCCGATCCCATGTTCATCA CGGATACCACCTTCCGTGACGGACAGCAGGCCCGGCCGCCCTATACGGTAAAGCAGATTTCCGAAATTTTTGACATGCTG CACCTGCTGGGCGGCATGAGCGGCCTCATCCGCGCGTCGGAATTTTTCATGTACTCCGACAAAGACAGGCGCGCCATAGA TGCCTGCCGCGCCAAAGGGTACAAATATCCGCAGATCACAGGCTGGATACGGGCCAATGAAAATGATCTGAAGATCGCAA AATCAATGGAATTCACCGAAGTGGGCATGCTCACATCGGTTTCGGATTACCACATACATCTCAAGCTGGGACTTGACCGG CAGCAGGCCATGGAAAACTACCTGCGCGTGATCGAAAAGGCGCTTGAATGGGGCATCACCCCGCGCTGCCATTTCGAAGA CATCACCCGCGCAGACATTCACGGCTTCTGCCTGCCCTTTGCCTCGCGCCTGATGGAACTGTCGCGCCAGAGCAAGCTGC CCGTCAAAATACGTCTGTGCGACACCATGGGATACGGTGTGCCCTATCCCGGCGCCACCCTGCCCCGTTCTGTTCCCCGT GTGGTGCGGGCCTTCACCGATGAAGCGGGCGTGCCGGGAGACTGGCTGGAATGGCACGGACACAACGACTTCCACAAGGT GCTGGTCAACGGCGTCACCGCATGGCTGTATGGCTGCGGCGGTGTCAACGGCACACTGATGGGCTTTGGCGAACGCACGG GCAACTCGCCCATAGAGGCGCTGGTCATCGAATACATTTCGCTTACGGGAGAGGATGACGCGGCCAACACGCATATCATT TCCGAAATAGGCGAATACTTCGAACGCGAACTTGAGTACCGCATTCCGGACAACTATCCGTTTGTGGGCAAAGACTTTAA CGCCACAAGCGCCGGTATCCATGTGGACGGGCTGAATAAAAACGAAGAGATCTACAACATCTTCGACACCCGCAAAATAC TCAACCGTTCCGTACCCATTATCATCACCGATAAATCGGGACGCGCAGGGGTCGCATACTGGATCAACAACTACCTGCGG GTGCCTGAAGGAAGACAGGTTTCCAAAACGCATCCCGCCGTGGGGCAGATATACAACCGCATCATGCAGGCTTATGAAGG CGGCCGCACCACGTCGTTCTCCAATCAGGAAATGACTAAACTGGTCAAACGCTACCTGCCCGAGCTTTTCGTCTCCGAAT TCGATTCGCTGAAGACGGTGGCCCGGCAGCTGGCATCCACCATCATTCTCAAGCTGGCCGCCGAATGTGTCATTGTACCG CAGAAAAGCGAGTATCCCTGCCTCAGCGATTTTGTGCGGCAGTTCTCGTTCATCCAGTACCTGTACCTCACCGACGTACG CGGCACGCTGCTGGCTTCCGTCATCACCGATCCGGCTTACAACGAGCGTTATCAGGAACTGCCGCTGGGCTACGACTTCT CCAACAGGCAATGGTTCCAGCGGCCCTCGCAGACCGGCGAACTGCATGTGACCGACCTGTACCAGTCACAGTTCACGGGC AAGCTTATTCTGACCGTTTCGGCACCGGTAACCGATGAAAATGACGACATCATGGGTATCGTGGGGGCGGACATCCAGCT GGAAGAACTGCTTAACCGCAAGGCCATGCTTGAAGATAATGAAAAAGACGCCTGA
Upstream 100 bases:
>100_bases TGGCCGTAAATCTGGCCCCCCGATGCGAAAAGGCGCGGCAGCTGCACAGGCAGGCCGTAACGCTGCAAAACAATTCATCC AACAGGAGGGCGATCGACGC
Downstream 100 bases:
>100_bases TCGGCCCTGCACAGACTGAACCGGCGCAGACTGCGCCGGCGCAACGACACGGCAACTGACGGGGGCGCGGAAAACCGCGC CCTCTTTTACTTGCCCGCAA
Product: HMGL-like domain-containing protein
Products: NA
Alternate protein names: Alpha-IPM synthase 2; Alpha-isopropylmalate synthase 2 [H]
Number of amino acids: Translated: 604; Mature: 603
Protein sequence:
>604_residues MSLLHLNRPVTRLSLQNREDPELYRDIFPYSKISRIAFDDIHIMPRPADPMFITDTTFRDGQQARPPYTVKQISEIFDML HLLGGMSGLIRASEFFMYSDKDRRAIDACRAKGYKYPQITGWIRANENDLKIAKSMEFTEVGMLTSVSDYHIHLKLGLDR QQAMENYLRVIEKALEWGITPRCHFEDITRADIHGFCLPFASRLMELSRQSKLPVKIRLCDTMGYGVPYPGATLPRSVPR VVRAFTDEAGVPGDWLEWHGHNDFHKVLVNGVTAWLYGCGGVNGTLMGFGERTGNSPIEALVIEYISLTGEDDAANTHII SEIGEYFERELEYRIPDNYPFVGKDFNATSAGIHVDGLNKNEEIYNIFDTRKILNRSVPIIITDKSGRAGVAYWINNYLR VPEGRQVSKTHPAVGQIYNRIMQAYEGGRTTSFSNQEMTKLVKRYLPELFVSEFDSLKTVARQLASTIILKLAAECVIVP QKSEYPCLSDFVRQFSFIQYLYLTDVRGTLLASVITDPAYNERYQELPLGYDFSNRQWFQRPSQTGELHVTDLYQSQFTG KLILTVSAPVTDENDDIMGIVGADIQLEELLNRKAMLEDNEKDA
Sequences:
>Translated_604_residues MSLLHLNRPVTRLSLQNREDPELYRDIFPYSKISRIAFDDIHIMPRPADPMFITDTTFRDGQQARPPYTVKQISEIFDML HLLGGMSGLIRASEFFMYSDKDRRAIDACRAKGYKYPQITGWIRANENDLKIAKSMEFTEVGMLTSVSDYHIHLKLGLDR QQAMENYLRVIEKALEWGITPRCHFEDITRADIHGFCLPFASRLMELSRQSKLPVKIRLCDTMGYGVPYPGATLPRSVPR VVRAFTDEAGVPGDWLEWHGHNDFHKVLVNGVTAWLYGCGGVNGTLMGFGERTGNSPIEALVIEYISLTGEDDAANTHII SEIGEYFERELEYRIPDNYPFVGKDFNATSAGIHVDGLNKNEEIYNIFDTRKILNRSVPIIITDKSGRAGVAYWINNYLR VPEGRQVSKTHPAVGQIYNRIMQAYEGGRTTSFSNQEMTKLVKRYLPELFVSEFDSLKTVARQLASTIILKLAAECVIVP QKSEYPCLSDFVRQFSFIQYLYLTDVRGTLLASVITDPAYNERYQELPLGYDFSNRQWFQRPSQTGELHVTDLYQSQFTG KLILTVSAPVTDENDDIMGIVGADIQLEELLNRKAMLEDNEKDA >Mature_603_residues SLLHLNRPVTRLSLQNREDPELYRDIFPYSKISRIAFDDIHIMPRPADPMFITDTTFRDGQQARPPYTVKQISEIFDMLH LLGGMSGLIRASEFFMYSDKDRRAIDACRAKGYKYPQITGWIRANENDLKIAKSMEFTEVGMLTSVSDYHIHLKLGLDRQ QAMENYLRVIEKALEWGITPRCHFEDITRADIHGFCLPFASRLMELSRQSKLPVKIRLCDTMGYGVPYPGATLPRSVPRV VRAFTDEAGVPGDWLEWHGHNDFHKVLVNGVTAWLYGCGGVNGTLMGFGERTGNSPIEALVIEYISLTGEDDAANTHIIS EIGEYFERELEYRIPDNYPFVGKDFNATSAGIHVDGLNKNEEIYNIFDTRKILNRSVPIIITDKSGRAGVAYWINNYLRV PEGRQVSKTHPAVGQIYNRIMQAYEGGRTTSFSNQEMTKLVKRYLPELFVSEFDSLKTVARQLASTIILKLAAECVIVPQ KSEYPCLSDFVRQFSFIQYLYLTDVRGTLLASVITDPAYNERYQELPLGYDFSNRQWFQRPSQTGELHVTDLYQSQFTGK LILTVSAPVTDENDDIMGIVGADIQLEELLNRKAMLEDNEKDA
Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]
COG id: COG0119
COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]
Homologues:
Organism=Escherichia coli, GI1786261, Length=361, Percent_Identity=25.4847645429363, Blast_Score=89, Evalue=1e-18, Organism=Saccharomyces cerevisiae, GI6320071, Length=338, Percent_Identity=24.8520710059172, Blast_Score=67, Evalue=7e-12, Organism=Saccharomyces cerevisiae, GI6320019, Length=324, Percent_Identity=25, Blast_Score=67, Evalue=1e-11,
Paralogues:
None
Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR002034 - InterPro: IPR013785 - InterPro: IPR013477 - InterPro: IPR000891 [H]
Pfam domain/function: PF00682 HMGL-like [H]
EC number: =2.3.3.13 [H]
Molecular weight: Translated: 68850; Mature: 68719
Theoretical pI: Translated: 5.79; Mature: 5.79
Prosite motif: PS50991 PYR_CT
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.2 %Cys (Translated Protein) 2.6 %Met (Translated Protein) 3.8 %Cys+Met (Translated Protein) 1.2 %Cys (Mature Protein) 2.5 %Met (Mature Protein) 3.6 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MSLLHLNRPVTRLSLQNREDPELYRDIFPYSKISRIAFDDIHIMPRPADPMFITDTTFRD CCCEECCCCHHHHCCCCCCCHHHHHHHCCHHHHHHHHHCCEEECCCCCCCEEEECCCCCC GQQARPPYTVKQISEIFDMLHLLGGMSGLIRASEFFMYSDKDRRAIDACRAKGYKYPQIT CCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHEEECCCCHHHHHHHHHCCCCCCCEE GWIRANENDLKIAKSMEFTEVGMLTSVSDYHIHLKLGLDRQQAMENYLRVIEKALEWGIT EEEEECCCCHHHHHCCCHHHHHHHEECCCEEEEEEECCCHHHHHHHHHHHHHHHHHHCCC PRCHFEDITRADIHGFCLPFASRLMELSRQSKLPVKIRLCDTMGYGVPYPGATLPRSVPR CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCHHHHH VVRAFTDEAGVPGDWLEWHGHNDFHKVLVNGVTAWLYGCGGVNGTLMGFGERTGNSPIEA HHHHHHHCCCCCCCHHEECCCCHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCHHHH LVIEYISLTGEDDAANTHIISEIGEYFERELEYRIPDNYPFVGKDFNATSAGIHVDGLNK HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCCC NEEIYNIFDTRKILNRSVPIIITDKSGRAGVAYWINNYLRVPEGRQVSKTHPAVGQIYNR CCHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHCEEECCCCCCCCCCCCHHHHHHHH IMQAYEGGRTTSFSNQEMTKLVKRYLPELFVSEFDSLKTVARQLASTIILKLAAECVIVP HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEC QKSEYPCLSDFVRQFSFIQYLYLTDVRGTLLASVITDPAYNERYQELPLGYDFSNRQWFQ CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCHHHH RPSQTGELHVTDLYQSQFTGKLILTVSAPVTDENDDIMGIVGADIQLEELLNRKAMLEDN CCCCCCCEEEEEEHHHCCCCEEEEEEECCCCCCCCCEEEEECCCEEHHHHHCHHHHCCCC EKDA CCCC >Mature Secondary Structure SLLHLNRPVTRLSLQNREDPELYRDIFPYSKISRIAFDDIHIMPRPADPMFITDTTFRD CCEECCCCHHHHCCCCCCCHHHHHHHCCHHHHHHHHHCCEEECCCCCCCEEEECCCCCC GQQARPPYTVKQISEIFDMLHLLGGMSGLIRASEFFMYSDKDRRAIDACRAKGYKYPQIT CCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHEEECCCCHHHHHHHHHCCCCCCCEE GWIRANENDLKIAKSMEFTEVGMLTSVSDYHIHLKLGLDRQQAMENYLRVIEKALEWGIT EEEEECCCCHHHHHCCCHHHHHHHEECCCEEEEEEECCCHHHHHHHHHHHHHHHHHHCCC PRCHFEDITRADIHGFCLPFASRLMELSRQSKLPVKIRLCDTMGYGVPYPGATLPRSVPR CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCHHHHH VVRAFTDEAGVPGDWLEWHGHNDFHKVLVNGVTAWLYGCGGVNGTLMGFGERTGNSPIEA HHHHHHHCCCCCCCHHEECCCCHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCHHHH LVIEYISLTGEDDAANTHIISEIGEYFERELEYRIPDNYPFVGKDFNATSAGIHVDGLNK HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCCC NEEIYNIFDTRKILNRSVPIIITDKSGRAGVAYWINNYLRVPEGRQVSKTHPAVGQIYNR CCHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHCEEECCCCCCCCCCCCHHHHHHHH IMQAYEGGRTTSFSNQEMTKLVKRYLPELFVSEFDSLKTVARQLASTIILKLAAECVIVP HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEC QKSEYPCLSDFVRQFSFIQYLYLTDVRGTLLASVITDPAYNERYQELPLGYDFSNRQWFQ CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCHHHH RPSQTGELHVTDLYQSQFTGKLILTVSAPVTDENDDIMGIVGADIQLEELLNRKAMLEDN CCCCCCCEEEEEEHHHCCCCEEEEEEECCCCCCCCCEEEEECCCEEHHHHHCHHHHCCCC EKDA CCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 11997336 [H]