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The map label for this gene is nylA [H]

Identifier: 78355565

GI number: 78355565

Start: 525303

End: 526799

Strand: Reverse

Name: nylA [H]

Synonym: Dde_0518

Alternate gene names: 78355565

Gene position: 526799-525303 (Counterclockwise)

Preceding gene: 78355566

Following gene: 78355563

Centisome position: 14.12

GC content: 63.66

Gene sequence:

>1497_bases
ATGACCACGCAGTCAGACACCCCGCCATATGCCGCCCTTTCCATGCAGGAACTGGGAACACTGGATGCCGTGGCCCTTCG
GGGGCTTATCCGCTCCGGCCAGATTACGCCGGACGAAGTGCTGCATACCGTATTTCAAGCCATGGAGGCACTGAATCCGG
CCATCAACGCCGTGGTGCAGCCCATGAAGACGCAGGCATATGCCGAACTGCGGCATATTGATCCGGAAGCGGCTCTCTAC
GGAGTACCGTATCTGCTCAAAGACCTGGGCCCGTCATACGCGGGTGTGCCCACCCGCATGGCGTGCATGGCGCTGAAAGA
CGGCTTTTTTCCCGGCGAAGACAGCCACCTGACACACCGCTTCCGCACGCTGGGCATGGTGCCGGCAGGCAAAAGCGCAA
CGCCGGAATTCGGCCTGACAGCCACCACGGACAGTGTGCTGCACGGCCAGACACGCAACCCGTGGGATGTGACCCGTTCT
CCGGGCGGTTCCAGCGGTGGTTCAGCTTCTGCCGTGGCAGCCGGTATCGTACCCGTTGCACATGGCAACGACGGGGGCGG
CTCCATACGCATTCCTGCTGCAGCCTGCGGTCTGGTGGGCTTCAAATGCAGCCGGGGTCTGCTTTCATGGGCGCCGGAGC
TGGGAGAATGCTGGGGCGCGCTTGCCACCAACGGCGTGCTGACACGCACCGTACGCGACAGTGCGGCAGCCCTTGACGGG
CTGTGCGGCACAGGGCTGGGCGACCCGTATCCTTTTCCGCGGCCGCCGCTGCCGTTCAGTACCGAAATACGCAGAGCAAC
CGGACGGCTGAAGATAGGATACGCCCTGCAGTGGCCCGCCGGAGCGGCCCCCGCGTCCGAAGACTGCCGCAATGCGGTCA
TGCACACGGCCCGTCTGCTGGAAGGCATGGGACACCATGTGGAAGAAGCAGCTCCCGACCTGCACTGCGACGAAGTGGCC
GACATTTTCATGACCATTGTCGCCGCACAGGTGATAGTCACGCTGGGCCCGCTCATTTCGCCCGCAGGCATGCCGGATGA
ACGCCATGCCGAAAAACTGACCCGCCTTCTGTGGGAAAAAGGGCAGCGCGAAACCGGCGCACACTATGCGGGTGCACTCA
ACCGCATGCACCTGCTGGGAAGGGCCATGGCCCGCTTTTTCGAGCGCTACGACGTGCTGCTGACGCCTGTCATGACCACG
CCCCCCGCAAAGCTGGGCGCATATTCCCTGCAGCAGATGGAGCTGGACGACTATGCCGCCATGCTGGGAACATTCATTCC
CTATACGCCGCCCTTCAACATGGCCGGTTCACCCGCCGTATCGCTGCCGCTGTATGCCACTGAACACGGTCTGCCCGTGG
GTGTTCAGCTGGGCGCAGGCACAGGCCGCGACGGGCTGCTGTTCCGTCTGGCTGCCCGGCTGGAGGAGGCCGCTCCGTGG
CACACACGCGTTCCCGCCGTGCATGTTTCACGGCTGGAAACCACAGGCAGACACTAA

Upstream 100 bases:

>100_bases
CCTTCCGCAGGAGGGCTTCCCTTTTTATATGCCGCAGCCATTGTTAATTATTTAAAAACGCGTATCATGATTCCGGTGAC
AATATAACCGGAGGTTTACC

Downstream 100 bases:

>100_bases
CCCCGCAGCACACGGCAAAACAAGAAAAGCCGCCCCTGCTCTGCAGCGGGCGGCTTCTCATCCGGACGGAGTGCGCAATG
CTATTCGCCGTTCAGCTCTG

Product: 6-aminohexanoate-cyclic-dimer hydrolase

Products: NA

Alternate protein names: Nylon oligomers-degrading enzyme EI [H]

Number of amino acids: Translated: 498; Mature: 497

Protein sequence:

>498_residues
MTTQSDTPPYAALSMQELGTLDAVALRGLIRSGQITPDEVLHTVFQAMEALNPAINAVVQPMKTQAYAELRHIDPEAALY
GVPYLLKDLGPSYAGVPTRMACMALKDGFFPGEDSHLTHRFRTLGMVPAGKSATPEFGLTATTDSVLHGQTRNPWDVTRS
PGGSSGGSASAVAAGIVPVAHGNDGGGSIRIPAAACGLVGFKCSRGLLSWAPELGECWGALATNGVLTRTVRDSAAALDG
LCGTGLGDPYPFPRPPLPFSTEIRRATGRLKIGYALQWPAGAAPASEDCRNAVMHTARLLEGMGHHVEEAAPDLHCDEVA
DIFMTIVAAQVIVTLGPLISPAGMPDERHAEKLTRLLWEKGQRETGAHYAGALNRMHLLGRAMARFFERYDVLLTPVMTT
PPAKLGAYSLQQMELDDYAAMLGTFIPYTPPFNMAGSPAVSLPLYATEHGLPVGVQLGAGTGRDGLLFRLAARLEEAAPW
HTRVPAVHVSRLETTGRH

Sequences:

>Translated_498_residues
MTTQSDTPPYAALSMQELGTLDAVALRGLIRSGQITPDEVLHTVFQAMEALNPAINAVVQPMKTQAYAELRHIDPEAALY
GVPYLLKDLGPSYAGVPTRMACMALKDGFFPGEDSHLTHRFRTLGMVPAGKSATPEFGLTATTDSVLHGQTRNPWDVTRS
PGGSSGGSASAVAAGIVPVAHGNDGGGSIRIPAAACGLVGFKCSRGLLSWAPELGECWGALATNGVLTRTVRDSAAALDG
LCGTGLGDPYPFPRPPLPFSTEIRRATGRLKIGYALQWPAGAAPASEDCRNAVMHTARLLEGMGHHVEEAAPDLHCDEVA
DIFMTIVAAQVIVTLGPLISPAGMPDERHAEKLTRLLWEKGQRETGAHYAGALNRMHLLGRAMARFFERYDVLLTPVMTT
PPAKLGAYSLQQMELDDYAAMLGTFIPYTPPFNMAGSPAVSLPLYATEHGLPVGVQLGAGTGRDGLLFRLAARLEEAAPW
HTRVPAVHVSRLETTGRH
>Mature_497_residues
TTQSDTPPYAALSMQELGTLDAVALRGLIRSGQITPDEVLHTVFQAMEALNPAINAVVQPMKTQAYAELRHIDPEAALYG
VPYLLKDLGPSYAGVPTRMACMALKDGFFPGEDSHLTHRFRTLGMVPAGKSATPEFGLTATTDSVLHGQTRNPWDVTRSP
GGSSGGSASAVAAGIVPVAHGNDGGGSIRIPAAACGLVGFKCSRGLLSWAPELGECWGALATNGVLTRTVRDSAAALDGL
CGTGLGDPYPFPRPPLPFSTEIRRATGRLKIGYALQWPAGAAPASEDCRNAVMHTARLLEGMGHHVEEAAPDLHCDEVAD
IFMTIVAAQVIVTLGPLISPAGMPDERHAEKLTRLLWEKGQRETGAHYAGALNRMHLLGRAMARFFERYDVLLTPVMTTP
PAKLGAYSLQQMELDDYAAMLGTFIPYTPPFNMAGSPAVSLPLYATEHGLPVGVQLGAGTGRDGLLFRLAARLEEAAPWH
TRVPAVHVSRLETTGRH

Specific function: Specifically catalyzes the hydrolysis of 6-aminohexanoic acid cyclic dimer (1,8-diazacyclotetradecane-2,9-dione) to form the linear dimer 6-aminohexanoyl-6-aminohexanoic acid. Is inactive on 6-aminohexanoic acid oligomers (degree of polymerization 2 to 6)

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=518, Percent_Identity=24.7104247104247, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI195972892, Length=243, Percent_Identity=25.9259259259259, Blast_Score=80, Evalue=4e-15,
Organism=Homo sapiens, GI166795287, Length=278, Percent_Identity=29.8561151079137, Blast_Score=79, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17538252, Length=314, Percent_Identity=28.0254777070064, Blast_Score=97, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17556264, Length=339, Percent_Identity=27.7286135693215, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI71990152, Length=543, Percent_Identity=26.7034990791897, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17538254, Length=351, Percent_Identity=27.9202279202279, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17556276, Length=321, Percent_Identity=26.1682242990654, Blast_Score=72, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17556278, Length=321, Percent_Identity=26.1682242990654, Blast_Score=72, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI32565337, Length=358, Percent_Identity=25.9776536312849, Blast_Score=69, Evalue=6e-12,
Organism=Drosophila melanogaster, GI161078093, Length=505, Percent_Identity=25.3465346534653, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24648113, Length=495, Percent_Identity=31.1111111111111, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24644968, Length=509, Percent_Identity=25.5402750491159, Blast_Score=105, Evalue=8e-23,
Organism=Drosophila melanogaster, GI45550774, Length=500, Percent_Identity=24.8, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24648435, Length=496, Percent_Identity=24.7983870967742, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24648437, Length=496, Percent_Identity=24.7983870967742, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI21356731, Length=523, Percent_Identity=23.7093690248566, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24652985, Length=201, Percent_Identity=29.3532338308458, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19922090, Length=201, Percent_Identity=29.3532338308458, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24652981, Length=201, Percent_Identity=29.3532338308458, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24652983, Length=201, Percent_Identity=29.3532338308458, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24648441, Length=426, Percent_Identity=23.7089201877934, Blast_Score=70, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24648439, Length=426, Percent_Identity=23.7089201877934, Blast_Score=70, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: =3.5.2.12 [H]

Molecular weight: Translated: 52932; Mature: 52801

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTQSDTPPYAALSMQELGTLDAVALRGLIRSGQITPDEVLHTVFQAMEALNPAINAVVQ
CCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCHHHHHHHH
PMKTQAYAELRHIDPEAALYGVPYLLKDLGPSYAGVPTRMACMALKDGFFPGEDSHLTHR
HHHHHHHHHHHHCCHHHHHHCCHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHH
FRTLGMVPAGKSATPEFGLTATTDSVLHGQTRNPWDVTRSPGGSSGGSASAVAAGIVPVA
HHHHCCCCCCCCCCCCCCCEECCCHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHCCEEEE
HGNDGGGSIRIPAAACGLVGFKCSRGLLSWAPELGECWGALATNGVLTRTVRDSAAALDG
CCCCCCCEEECCHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHC
LCGTGLGDPYPFPRPPLPFSTEIRRATGRLKIGYALQWPAGAAPASEDCRNAVMHTARLL
HHCCCCCCCCCCCCCCCCCCHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHH
EGMGHHVEEAAPDLHCDEVADIFMTIVAAQVIVTLGPLISPAGMPDERHAEKLTRLLWEK
HHHCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHC
GQRETGAHYAGALNRMHLLGRAMARFFERYDVLLTPVMTTPPAKLGAYSLQQMELDDYAA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
MLGTFIPYTPPFNMAGSPAVSLPLYATEHGLPVGVQLGAGTGRDGLLFRLAARLEEAAPW
HHHHHCCCCCCCCCCCCCCEECCEEECCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCC
HTRVPAVHVSRLETTGRH
CCCCCHHHHHHHHHCCCC
>Mature Secondary Structure 
TTQSDTPPYAALSMQELGTLDAVALRGLIRSGQITPDEVLHTVFQAMEALNPAINAVVQ
CCCCCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCHHHHHHHH
PMKTQAYAELRHIDPEAALYGVPYLLKDLGPSYAGVPTRMACMALKDGFFPGEDSHLTHR
HHHHHHHHHHHHCCHHHHHHCCHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHH
FRTLGMVPAGKSATPEFGLTATTDSVLHGQTRNPWDVTRSPGGSSGGSASAVAAGIVPVA
HHHHCCCCCCCCCCCCCCCEECCCHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHCCEEEE
HGNDGGGSIRIPAAACGLVGFKCSRGLLSWAPELGECWGALATNGVLTRTVRDSAAALDG
CCCCCCCEEECCHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHC
LCGTGLGDPYPFPRPPLPFSTEIRRATGRLKIGYALQWPAGAAPASEDCRNAVMHTARLL
HHCCCCCCCCCCCCCCCCCCHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHH
EGMGHHVEEAAPDLHCDEVADIFMTIVAAQVIVTLGPLISPAGMPDERHAEKLTRLLWEK
HHHCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHC
GQRETGAHYAGALNRMHLLGRAMARFFERYDVLLTPVMTTPPAKLGAYSLQQMELDDYAA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
MLGTFIPYTPPFNMAGSPAVSLPLYATEHGLPVGVQLGAGTGRDGLLFRLAARLEEAAPW
HHHHHCCCCCCCCCCCCCCEECCEEECCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCC
HTRVPAVHVSRLETTGRH
CCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2722746 [H]